Anti-Human Secondary Antibodies

The Thermo Scientific Pierce Anti-Human Secondary Antibodies are affinity-purified polyclonal antibodies with well-characterized specificity for human immunoglobulin classes, subclasses, and fragments. They are available as unlabeled antibodies as well as conjugates of alkaline phosphatase (AP), horseradish peroxidase (HRP), Dylight fluorescent conjugates, and biotin. Host species include goat, rabbit, mouse, chicken, cow, rat and sheep.

Thermo Scientific Pierce secondary antibodies are available as whole IgG or the F(ab')2 fragment. Each of these antibodies is affinity purified using its target antigen (the primary antibody class, subclass, and fragment) coupled to an agarose support. This affinity purification eliminates nonspecific antibodies, resulting in high specificity and low background signal. Conjugated antibodies are affinity purified before labeling.

Selected secondary antibodies have been further purified by adsorbing the antigen-purified antibody to an affinity column containing immobilized serum proteins of selected species. This additional processing minimizes antibody cross-reactivity to the serum proteins of those selected species. These antibodies are designated as Cross Adsorbed.

Use the filter search on the left to find your desired anti-human secondary antibody by host, conjugate, class, type, target species, clone, or application.
Filter Results
By Class
Polyclonal (139)
Monoclonal (52)
Control (2)
By Host
Goat (72)
Mouse (55)
Rabbit (28)
By Application
WB (124)
FACS (124)
ICC (100)
By Clone
CH2 (4)
HP6025 (2)
BE5 (2)
By Label
Labeled (123)
Not Labeled (70)
HRP (25)
Product Description Host Target Species Applications Imgs / Refs Size Product # Price
Human IgG, Whole Molecule Control, HRP conjugate Human N/A IF, ICC, IHC, BLOCK, Ctrl 00 1 mg 31465 $75.00
Human Gamma Globulin Isotype Control Human N/A FACS, IP, ChIP, Ctrl 00 10 mg 31879 $50.00
Goat anti-Human IgG (Heavy Chain) Secondary Antibody Goat Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug 31118 $185.00
Goat anti-Human IgG (H+L) Cross Adsorbed Secondary Antibody Goat Hu WB, IF, ICC, IHC, FACS, IP 00 1.5 mg 31119 $99.00
Goat anti-Human IgG F(ab')2 Secondary Antibody Goat Hu WB, IF, ICC, IHC, FACS, IP 00 2 mg 31122 $120.00
Goat anti-Human IgM Secondary Antibody Goat Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug 31124 $185.00
Goat anti-Human IgG Fc Cross Adsorbed Secondary Antibody Goat Hu WB, IF, ICC, IHC, FACS, IP, ELISA 01 1 mg 31125 $95.00
Goat anti-Human IgG, IgM, IgA (H+L) Secondary Antibody Goat Hu WB, IF, ICC, IHC, FACS, IP 00 2 mg 31128 $130.00
Goat anti-Human IgG (Kappa light chain) Secondary Antibody Goat Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug 31129 $185.00
Goat anti-Human IgG (H+L) Secondary Antibody Goat Hu WB, IF, ICC, IHC, FACS, IP 00 2 mg 31130 $99.00
Goat anti-Human IgG (Lambda light chain) Secondary Antibody Goat Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug 31131 $185.00
Goat anti-Human IgG F(ab')2 Cross Adsorbed Secondary Antibody Goat Hu WB, IF, ICC, IHC, FACS, IP 00 1.5 mg 31132 $110.00
Mouse anti-Human IgG (H+L) Cross Adsorbed Secondary Antibody Mouse Hu WB, IF, ICC, IHC, FACS, IP 00 2 mg 31135 $130.00
Goat anti-Human IgM Secondary Antibody Goat Hu WB, IF, ICC, IHC, FACS, IP 00 2 mg 31136 $110.00
Mouse anti-Human IgG (H+L) Cross Adsorbed Secondary Antibody Mouse Hu WB, IF, ICC, IHC, FACS, IP 00 1.5 mg 31137 $125.00
Goat anti-Human IgA (Alpha heavy chain) Secondary Antibody Goat Hu WB, IF, ICC, IHC, FACS, IP 00 2 mg 31140 $110.00
Rabbit anti-Human IgG Fc Secondary Antibody Rabbit Hu WB, IF, ICC, IHC, FACS, IP 00 2 mg 31142 $120.00
Rabbit anti-Human IgG (H+L) Secondary Antibody Rabbit Hu WB, IF, ICC, IHC, FACS, IP 00 2 mg 31143 $120.00
F(ab')2-Goat anti-Human IgG Fc gamma Secondary Antibody F(ab')2-Goat Hu WB, IF, ICC, IHC, FACS, IP 00 1 mg 31163 $140.00
F(ab')2-Goat anti-Human IgG (H+L) Secondary Antibody F(ab')2-Goat Hu WB, IF, ICC, IHC, FACS, IP 00 1 mg 31165 $99.00
Goat anti-Human IgG (H+L) Secondary Antibody, AP conjugate Goat Hu WB, IF, ICC, IHC, FACS, IP 00 1 ml 31310 $170.00
Goat anti-Human IgG F(ab')2 Secondary Antibody, AP conjugate Goat Hu WB, IF, ICC, IHC, FACS, IP 00 1 ml 31312 $199.00
Goat anti-Human IgA (Alpha heavy chain) Secondary Antibody, AP conjugate Goat Hu WB, IF, ICC, IHC, FACS, IP 00 1 ml 31314 $185.00
Goat anti-Human IgG, IgM, IgA (H+L) Secondary Antibody, AP conjugate Goat Hu WB, IF, ICC, IHC, FACS, IP 00 1 ml 31316 $185.00
Rabbit anti-Human IgG Fc Secondary Antibody, AP conjugate Rabbit Hu WB, IF, ICC, IHC, FACS, IP 00 1 ml 31318 $185.00
Goat anti-Human IgG (H+L) Secondary Antibody, HRP conjugate Goat Hu WB, IF, ICC, IHC, FACS, IP 00 2 ml 31410 $140.00
Goat anti-Human IgG (H+L) Cross Adsorbed Secondary Antibody, HRP conjugate Goat Hu WB, IF, ICC, IHC, FACS, IP 00 1.5 ml 31412 $140.00
Goat anti-Human IgG Fc Cross Adsorbed Secondary Antibody, HRP conjugate Goat Hu WB, IF, ICC, IHC, FACS, IP 00 1 ml 31413 $170.00
Goat anti-Human IgG F(ab')2 Cross Adsorbed Secondary Antibody, HRP conjugate Goat Hu WB, IF, ICC, IHC, FACS, IP 00 1.5 ml 31414 $180.00
Goat anti-Human IgM Secondary Antibody, HRP conjugate Goat Hu WB, IF, ICC, IHC, FACS, IP 00 2 ml 31415 $185.00
Goat anti-Human IgA (Alpha heavy chain) Secondary Antibody, HRP conjugate Goat Hu WB, IF, ICC, IHC, FACS, IP 00 2 ml 31417 $165.00
Goat anti-Human IgG, IgM, IgA (H+L) Secondary Antibody, HRP conjugate Goat Hu WB, IF, ICC, IHC, FACS, IP 00 2 ml 31418 $165.00
Mouse anti-Human IgG (H+L) Cross Adsorbed Secondary Antibody, HRP conjugate Mouse Hu WB, IF, ICC, IHC, FACS, IP 00 1.5 ml 31420 $135.00
Rabbit anti-Human IgG Fc Secondary Antibody, HRP conjugate Rabbit Hu WB, IF, ICC, IHC, FACS, IP 00 1.5 ml 31423 $185.00
Goat anti-Human IgG F(ab')2 Secondary Antibody, HRP conjugate Goat Hu WB, IF, ICC, IHC, FACS, IP 10 2 ml 31482 $170.00
Goat anti-Human IgG (H+L) Secondary Antibody, FITC conjugate Goat Hu IF, ICC, IHC, FACS, IP 00 2 mg 31529 $105.00
Goat anti-Human IgG (H+L) Cross Adsorbed Secondary Antibody, FITC conjugate Goat Hu IF, ICC, IHC, FACS, IP 00 1.5 mg 31531 $105.00
Rabbit anti-Human IgG Fc Secondary Antibody, FITC conjugate Rabbit Hu IF, ICC, IHC, FACS, IP 00 1.5 mg 31535 $125.00
F(ab')2-Goat anti-Human IgG, IgM, IgA (H+L) Secondary Antibody, FITC conjugate F(ab')2-Goat Hu IF, ICC, IHC, FACS, IP 00 1 mg 31539 $145.00
Goat anti-Human IgM Secondary Antibody, FITC conjugate Goat Hu IF, ICC, IHC, FACS, IP 00 2 mg 31575 $140.00
Goat anti-Human IgA (Alpha heavy chain) Secondary Antibody, FITC conjugate Goat Hu IF, ICC, IHC, FACS, IP 00 2 mg 31577 $110.00
Goat anti-Human IgG F(ab')2 Secondary Antibody, FITC conjugate Goat Hu IF, ICC, IHC, FACS, IP 00 2 mg 31628 $125.00
Goat anti-Human IgG (H+L) Secondary Antibody, Rhodamine conjugate Goat Hu IF, ICC, IHC, FACS, IP 00 2 mg 31656 $115.00
Goat anti-Human IgG (H+L) Cross Adsorbed Secondary Antibody, Rhodamine conjugate Goat Hu IF, ICC, IHC, FACS, IP 00 1.5 mg 31683 $115.00
Goat anti-Human IgG F(ab')2 Secondary Antibody, Rhodamine conjugate Goat Hu IF, ICC, IHC, FACS, IP 00 2 mg 31684 $120.00
Goat anti-Human IgG (H+L) Secondary Antibody, Biotin conjugate Goat Hu WB, IF, ICC, IHC, FACS, IP, ELISA 00 1.5 mg 31770 $185.00
Goat anti-Human IgG (H+L) Cross Adsorbed Secondary Antibody, Biotin conjugate Goat Hu WB, IF, ICC, IHC, FACS, IP, ELISA 00 1.5 ml 31774 $135.00
Goat anti-Human IgM Secondary Antibody, Biotin conjugate Goat Hu WB, IF, ICC, IHC, FACS, IP, ELISA 00 500 ug 31778 $185.00
Goat anti-Human IgG (Kappa light chain) Secondary Antibody, Biotin conjugate Goat Hu WB, IF, ICC, IHC, FACS, IP, ELISA 00 500 ug 31780 $185.00
Goat anti-Human IgG, IgM, IgA (H+L) Secondary Antibody, Biotin conjugate Goat Hu WB, IF, ICC, IHC, FACS, IP, ELISA 00 2 ml 31782 $170.00
Mouse anti-Human IgG (H+L) Secondary Antibody, Biotin conjugate Mouse Hu WB, IF, ICC, IHC, FACS, IP, ELISA 00 1 ml 31784 $150.00
Rabbit anti-Human IgG (H+L) Secondary Antibody, Biotin conjugate Rabbit Hu WB, IF, ICC, IHC, FACS, IP, ELISA 00 1.5 ml 31786 $135.00
Rabbit anti-Human IgG Fc Secondary Antibody, Biotin conjugate Rabbit Hu WB, IF, ICC, IHC, FACS, IP, ELISA 00 1.5 ml 31789 $165.00
Goat anti-Human IgM Cross Adsorbed Secondary Antibody, DyLight 350 conjugate Goat Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10101 $145.00
Goat anti-Human IgM Cross Adsorbed Secondary Antibody, DyLight 488 conjugate Goat Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10102 $145.00
Goat anti-Human IgM Cross Adsorbed Secondary Antibody, DyLight 550 conjugate Goat Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10103 $145.00
Goat anti-Human IgM Cross Adsorbed Secondary Antibody, DyLight 594 conjugate Goat Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10104 $145.00
Goat anti-Human IgM Cross Adsorbed Secondary Antibody, DyLight 650 conjugate Goat Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10105 $145.00
Goat anti-Human IgM Cross Adsorbed Secondary Antibody, DyLight 680 conjugate Goat Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10106 $145.00
Goat anti-Human IgM Cross Adsorbed Secondary Antibody, DyLight 755 conjugate Goat Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10107 $145.00
Goat anti-Human IgM Cross Adsorbed Secondary Antibody, DyLight 800 conjugate Goat Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10108 $145.00
Rabbit anti-Human IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 350 conjugate Rabbit Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10109 $145.00
Rabbit anti-Human IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 488 conjugate Rabbit Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10110 $145.00
Rabbit anti-Human IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 550 conjugate Rabbit Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10111 $145.00
Rabbit anti-Human IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 594 conjugate Rabbit Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10112 $145.00
Rabbit anti-Human IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 650 conjugate Rabbit Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10113 $145.00
Rabbit anti-Human IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 680 conjugate Rabbit Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10114 $145.00
Rabbit anti-Human IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 755 conjugate Rabbit Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10115 $145.00
Rabbit anti-Human IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 800 conjugate Rabbit Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10116 $145.00
Goat anti-Human IgG (H+L), Cross Adsorbed Secondary Antibody, DyLight 350 conjugate Goat Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10117 $145.00
Goat anti-Human IgG (H+L), Cross Adsorbed Secondary Antibody, DyLight 550 conjugate Goat Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10119 $145.00
Goat anti-Human IgG (H+L), Cross Adsorbed Secondary Antibody, DyLight 650 conjugate Goat Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10121 $145.00
Goat anti-Human IgG (H+L), Cross Adsorbed Secondary Antibody, DyLight 680 conjugate Goat Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10122 $145.00
Goat anti-Human IgG (H+L), Cross Adsorbed Secondary Antibody, DyLight 800 conjugate Goat Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10124 $145.00
Donkey anti-Human IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 350 conjugate Donkey Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10125 $145.00
Donkey anti-Human IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 488 conjugate Donkey Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10126 $145.00
Donkey anti-Human IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 550 conjugate Donkey Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10127 $145.00
Donkey anti-Human IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 594 conjugate Donkey Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10128 $145.00
Donkey anti-Human IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 650 conjugate Donkey Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10129 $145.00
Donkey anti-Human IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 680 conjugate Donkey Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10130 $145.00
Donkey anti-Human IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 755 conjugate Donkey Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10131 $145.00
Donkey anti-Human IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 800 conjugate Donkey Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10132 $145.00
Goat anti-Human IgG Fc Cross Adsorbed Secondary Antibody, DyLight 350 conjugate Goat Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10133 $145.00
Goat anti-Human IgG Fc Cross Adsorbed Secondary Antibody, DyLight 488 conjugate Goat Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10134 $145.00
Goat anti-Human IgG Fc Cross Adsorbed Secondary Antibody, DyLight 550 conjugate Goat Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10135 $145.00
Goat anti-Human IgG Fc Cross Adsorbed Secondary Antibody, DyLight 594 conjugate Goat Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10136 $145.00
Goat anti-Human IgG Fc Cross Adsorbed Secondary Antibody, DyLight 650 conjugate Goat Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10137 $145.00
Goat anti-Human IgG Fc Cross Adsorbed Secondary Antibody, DyLight 680 conjugate Goat Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10138 $145.00
Goat anti-Human IgG Fc Cross Adsorbed Secondary Antibody, DyLight 755 conjugate Goat Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10139 $145.00
Goat anti-Human IgG Fc Cross Adsorbed Secondary Antibody, DyLight 800 conjugate Goat Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10140 $145.00
Goat anti-Human IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 488 conjugate Goat Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10142 $145.00
Goat anti-Human IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 550 conjugate Goat Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10143 $145.00
Goat anti-Human IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 680 conjugate Goat Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10146 $145.00
Goat anti-Human IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 800 conjugate Goat Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10148 $145.00
Mouse anti-Human IgM Secondary Antibody Mouse Hu WB, IHC (P), IP, ELISA 00 100 µl LF-MA0164 $270.00
Mouse anti-Human IgA Secondary Antibody Mouse Hu WB, IHC (P), IP, ELISA 00 100 µl LF-MA0165 $270.00
Mouse anti-Human IgE Secondary Antibody, FITC conjugate Mouse Hu FACS 00 100 ug MA1-10374 $190.00
Mouse anti-Human IgE Secondary Antibody, PE conjugate Mouse Hu FACS 00 100 ug MA1-10375 $225.00
Mouse anti-Human IgG Fab Secondary Antibody, PE conjugate Mouse Hu FACS 00 100 ug MA1-10377 $225.00
Mouse anti-Human IgG Fc Secondary Antibody Mouse Hu WB, ICC, ELISA 10 100 ug MA1-10378 $125.00
Mouse anti-Human IgG Fc Secondary Antibody, FITC conjugate Mouse Hu IF, ICC 00 100 ug MA1-10379 $190.00
Mouse anti-Human IgM Fc Secondary Antibody, PE conjugate Mouse Hu FACS 00 100 ug MA1-10381 $225.00
Mouse anti-Human IgG3 (Hinge region) Secondary Antibody Mouse Hu IHC (F), FACS, ELISA 00 100 µg MA1-34436 $340.00
Mouse anti-Human IgG4 Secondary Antibody, HRP conjugate Mouse Hu IHC (F), ELISA 00 100 µg MA1-34437 $475.00
Mouse anti-Human IgG1 Secondary Antibody Mouse Hu IHC (F), ELISA 00 1 mg MA1-34581 $450.00
Mouse anti-Human IgG2 Fab Secondary Antibody Mouse Hu IHC (F), ELISA 00 100 µg MA1-34755 $340.00
Mouse anti-Human IgG, IgM, IgA Fab Secondary Antibody Mouse Hu ELISA 00 1 mg MA1-34883 $399.00
Mouse anti-Human IgG (Kappa light chain) Secondary Antibody Mouse Hu IHC (P, F) 00 1 ml MA1-35486 $350.00
Mouse anti-Human IgG4 Secondary Antibody Mouse Hu IHC (P) 00 1 ml MA1-35518 $325.00
Mouse anti-Human IgA (Heavy chain) Secondary Antibody Mouse Hu WB, IHC (F), IP, ELISA 00 200 µg MA1-80150 $316.00
Mouse anti-Human IgG (Lambda light chain) Secondary Antibody, RPE conjugate Mouse Hu FACS 00 100 Tests MA1-80937 $316.00
Mouse anti-Human IgG Fc (CH2 domain) Secondary Antibody Mouse Hu, Nhp IHC (F), FACS, ELISA 00 200 µg MA1-81864 $316.00
Mouse anti-Human IgG4 Fc Secondary Antibody, HRP conjugate Mouse Hu, Nhp IHC (F), IHC (P), ELISA 00 200 µg MA1-82201 $775.00
Mouse anti-Human IgE Secondary Antibody Mouse Hu FACS, ELISA 10 1 mg MA1-82854 $360.00
Mouse anti-Human IgG1 Fc Secondary Antibody Mouse Hu ELISA 00 500 µg MA1-83240 $230.00
Mouse anti-Human IgG2 Secondary Antibody Mouse Hu ELISA 00 500 µg MA1-83241 $199.00
Mouse anti-Human IgG3 Secondary Antibody Mouse Hu ELISA 00 500 µg MA1-83242 $199.00
Mouse anti-Human IgG (CH3 domain) Secondary Antibody Mouse Hu WB, IHC (F), IHC (P), ELISA, HA 00 100 µg MA1-90268 $375.00
Rat anti-Human IgG2a Secondary Antibody Rat Hu IHC (F), FACS, ELISA 00 100 tests MA1-90900 $199.00
Mouse anti-Human IgD Secondary Antibody Mouse Hu IHC (F), IHC (P) 00 500 µl MA1-90969 $360.00
Mouse anti-Human IgE Secondary Antibody, AP conjugate Mouse Hu WB, ELISA 00 250 µl MA1-91884 $199.00
Mouse anti-Human IgA (Alpha heavy chain) Secondary Antibody Mouse Hu WB, IHC (P), FACS, ELISA 90 500 ul MA5-11208 $185.00
Mouse anti-human IgG Secondary Antibody Mouse Hu WB 10 100 ug MA5-14755 $316.00
Rabbit anti-Human IgG Secondary Antibody, Texas Red conjugate Rabbit Hu IHC (F), IHC (P), FACS, IM 00 1 mg PA1-28585 $185.00
Rabbit anti-Human IgG Secondary Antibody, HRP conjugate Rabbit Hu WB, IHC (F), IHC (P), ELISA, DB, IM 00 1 mg PA1-28587 $199.00
Sheep anti-Human IgG Secondary Antibody, HRP conjugate Sheep Hu WB, IHC (F), IHC (P), ELISA, IM 00 1 mg PA1-28652 $185.00
Goat anti-Human IgG Secondary Antibody, TRITC conjugate Goat Hu IHC (F), IHC (P), FACS, IM 00 100 µg PA1-28826 $160.00
Goat anti-Human IgG Secondary Antibody, Texas Red conjugate Goat Hu IHC (F), IHC (P), FACS, IM 00 100 µg PA1-28827 $140.00
Goat anti-Human IgG Secondary Antibody, AP conjugate Goat Hu WB, IHC (F), IHC (P), ELISA, DB, IM 00 100 µg PA1-28830 $140.00
Rabbit anti-Human IgG Secondary Antibody, TRITC conjugate Rabbit Hu IHC (F), IHC (P), FACS, IM 00 100 µg PA1-28833 $110.00
Rabbit anti-Human IgG Secondary Antibody, Texas Red conjugate Rabbit Hu IHC (F), IHC (P), FACS, IM 00 100 µg PA1-28834 $110.00
Rabbit anti-Human IgG Secondary Antibody, AP conjugate Rabbit Hu WB, IHC (F), IHC (P), ELISA, IM 00 100 µg PA1-28837 $140.00
Rabbit anti-Human IgG Secondary Antibody, AP conjugate Rabbit Hu WB, IHC (F), IHC (P), ELISA 00 1 mg PA1-30342 $340.00
Sheep anti-Human IgG Secondary Antibody, AP conjugate Sheep Hu WB, IHC (F), IHC (P), ELISA, IM 00 1 mg PA1-30368 $340.00
Goat anti-Human IgA Secondary Antibody, HRP conjugate Goat Hu WB, IHC (P), ELISA 00 1 ml PA1-74395 $175.00
Goat anti-Human IgG (Kappa light chain) Secondary Antibody, FITC conjugate Goat Hu IF, IHC (F), FACS 00 1 mg PA1-74405 $299.00
F(ab')2-Goat anti-Human IgG (Kappa light chain) Secondary Antibody, HRP conjugate F(ab')2-Goat Hu WB, IHC (P, F), ELISA 00 1 ml PA1-74407 $350.00
F(ab')2-Goat anti-Human IgG (Kappa light chain) Secondary Antibody, RPE conjugate F(ab')2-Goat Hu FACS 00 500 µl PA1-74408 $270.00
F(ab')2-Goat anti-Human IgG Secondary Antibody, RPE conjugate F(ab')2-Goat Hu FACS 00 1 ml PA1-84341 $380.00
Goat anti-Human IgG Fc Secondary Antibody Goat Hu WB, IP, ELISA 00 2 mg PA1-85606 $230.00
Goat anti-Human IgM Secondary Antibody, Biotin conjugate Goat Hu WB, FACS, ELISA 00 1 mg PA1-86071 $299.00
Goat anti-Human IgD Secondary Antibody, RPE conjugate Goat Hu FACS 00 500 µg PA1-86073 $460.00
Goat anti-Human IgG Secondary Antibody, RPE conjugate Goat Hu FACS 00 500 µg PA1-86078 $460.00
Goat anti-Human IgA Secondary Antibody, Biotin conjugate Goat Hu ELISA 00 1 mg PA1-86082 $299.00
Sheep anti-Human IgG3 Secondary Antibody, HRP conjugate Sheep Hu IHC (F), ELISA 00 1 ml PA1-86429 $1,050.00
Rabbit anti-Human IgA (Alpha heavy chain) Secondary Antibody Rabbit Hu WB, IHC (P), FACS 30 500 ul PA5-16388 $140.00
Rabbit anti-Human IgE (Heavy chain) Secondary Antibody Rabbit Hu IHC (P), FACS 20 500 ul PA5-16396 $140.00
Rabbit anti-Human IgD (Heavy chain) Secondary Antibody Rabbit Hu WB, IHC (P) 20 500 ul PA5-16400 $140.00
Sheep anti-Human IgA Secondary Antibody Sheep Ms, Rt, Bv IF 00 2 ml PA5-16923 $75.00
Chicken anti-Human IgE Secondary Antibody Chicken Hu WB 00 100 µg PY-10111 $316.00
Chicken anti-Human IgA Secondary Antibody Chicken Hu WB, IS, ELISA 00 100 µg PY-10126 $316.00
Mouse anti-Human Ig Mix Secondary Antibody Mouse Hu ELISA 00 1 mg SA1-10648 $316.00
Mouse anti-Human IgG (Lambda light chain) Secondary Antibody Mouse Hu IHC (F), FACS 00 100 µg SA1-12586 $255.00
Mouse anti-Human IgG (Kappa light chain) Secondary Antibody Mouse Hu WB, ICC, IHC (P), FACS, IP, ELISA 00 100 µg SA1-19155 $285.00
Mouse anti-Human IgG (Lambda light chain) Secondary Antibody Mouse Hu ICC, ELISA 00 100 µg SA1-19254 $245.00
Mouse anti-Human IgG Fab Secondary Antibody Mouse Hu WB, FACS, ELISA 10 100 µg SA1-19255 $199.00
Mouse anti-Human IgM Fc Secondary Antibody Mouse Hu WB, ICC, FACS, ELISA 00 100 µg SA1-19256 $199.00
Mouse anti-Human IgM Secondary Antibody Mouse Hu ELISA 00 100 µg SA1-19257 $175.00
Mouse anti-Human IgA Secondary Antibody Mouse Hu IHC (P), ELISA 10 100 µg SA1-19258 $155.00
Mouse anti-Human IgE Secondary Antibody Mouse Hu FACS, ELISA 20 100 µg SA1-19259 $215.00
Mouse anti-Human IgE Secondary Antibody Mouse Hu ELISA 00 100 µg SA1-19260 $185.00
Mouse anti-Human IgE Secondary Antibody Mouse Hu FACS, ELISA 00 100 µg SA1-19261 $175.00
Mouse anti-Human IgM Fc Secondary Antibody, APC conjugate Mouse Hu FACS 10 100 µg SA1-19468 $345.00
Mouse anti-Human IgM Fc Secondary Antibody, FITC conjugate Mouse Hu FACS 00 100 µg SA1-19598 $335.00
Mouse anti-Human IgM Secondary Antibody Mouse Hu WB 00 1 mg SA1-26391 $399.00
Mouse anti-Human IgM Secondary Antibody Mouse Hu ELISA 00 1 mg SA1-26392 $450.00
Mouse anti-Human IgA Secondary Antibody, HRP conjugate Mouse Hu IHC (F), ELISA, RIA, DB 00 1 ml SA1-35467 $750.00
Mouse anti-Human IgG Secondary Antibody Mouse Hu IHC (F), ELISA, RIA 00 1 ml SA1-35468 $399.00
Mouse anti-Human IgG Secondary Antibody, FITC conjugate Mouse Hu IHC, ELISA 00 1 ml SA1-35469 $630.00
Mouse anti-Human IgG Secondary Antibody, HRP conjugate Mouse Hu WB, IHC (F), ELISA 00 1 ml SA1-35470 $750.00
Mouse anti-Human IgM Secondary Antibody, HRP conjugate Mouse Hu IHC (F), ELISA, RIA, DB 00 1 ml SA1-35473 $899.00
Mouse anti-Human IgM Secondary Antibody Mouse Hu ICC, IHC (P, F) 00 1 ml SA1-35480 $350.00
Rabbit anti-Human IgA Secondary Antibody, FITC conjugate Rabbit Hu IHC 00 1 ml SA1-36096 $140.00
Rabbit anti-Human IgG Secondary Antibody, FITC conjugate Rabbit Hu IHC 00 1 ml SA1-36099 $165.00
Rabbit anti-Human IgM Secondary Antibody Rabbit Hu WB, IHC, ELISA 00 0.5 ml SA1-400 $199.00
Rabbit anti-Human IgG Fc gamma Secondary Antibody Rabbit Hu ELISA, IE 00 2 mg SA1-600 $170.00
Chicken anti-Human IgG Secondary Antibody Chicken Hu WB, ELISA 00 500 µg SA1-72036 $125.00
Chicken anti-Human IgG Secondary Antibody, FITC conjugate Chicken Hu IHC 00 500 µg SA1-72037 $175.00
Chicken anti-Human IgG Secondary Antibody, Biotin conjugate Chicken Hu WB, ELISA 00 500 µg SA1-72038 $155.00
Chicken anti-Human IgG Secondary Antibody, HRP conjugate Chicken Hu WB, ELISA 00 500 µg SA1-72039 $175.00
Chicken anti-Human IgG F(ab')2 Secondary Antibody Chicken Hu WB, ELISA 00 500 µg SA1-72043 $175.00
Chicken anti-Human IgG F(ab')2 Secondary Antibody, Biotin conjugate Chicken Hu WB, ELISA 00 500 µg SA1-72044 $285.00
Chicken anti-Human IgG F(ab')2 Secondary Antibody, HRP conjugate Chicken Hu WB, ELISA 00 500 µg SA1-72045 $285.00
Chicken anti-Human IgG Fc Secondary Antibody Chicken Hu WB, ELISA 00 500 µg SA1-72046 $175.00
Chicken anti-Human IgG Fc Secondary Antibody, FITC conjugate Chicken Hu IHC 00 500 µg SA1-72047 $175.00
Chicken anti-Human IgG Fc Secondary Antibody, Biotin conjugate Chicken Hu WB, ELISA 00 500 µg SA1-72048 $235.00
Chicken anti-Human IgG Fc Secondary Antibody, HRP conjugate Chicken Hu WB, ELISA 00 500 µg SA1-72049 $235.00
Chicken anti-Human IgM Secondary Antibody Chicken Hu WB, ELISA 00 500 µg SA1-72050 $125.00
Chicken anti-Human IgM Secondary Antibody, HRP conjugate Chicken Hu WB, ELISA 00 500 µg SA1-72053 $235.00
Mouse anti-Human IgA Secondary Antibody Mouse Hu WB, ELISA 00 100 µg SA1-72510 $175.00
Mouse anti-Human IgM Secondary Antibody Mouse Hu ELISA 00 100 µg SA1-72512 $175.00
Bovine anti-Human IgG Secondary Antibody Bovine Hu WB, ELISA 00 500 ug SA1-9501 $185.00
Bovine anti-Human IgG Secondary Antibody, Biotin conjugate Bovine Hu WB, ICC, IHC, ELISA 00 500 µg SA1-9506 $185.00
Product Images
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    Western Blot with anti-Human IgG F(ab')2 Polyclonal Secondary Antibody, HRP conjugate (31482)

    Western Blot with anti-Human IgG F(ab')2 Polyclonal Secondary Antibody, HRP conjugate (31482)

    Western blot analysis of humanized mouse Fab was performed by loading 100ug of L3.6pL pancreatic cancer cell lysate spiked with 2ug of humanized Fab (left lanes) or 100ug of L3.6pL cell lysate (right lanes) per well onto an SDS-PAGE gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5% milk in PBST for 4 hours. The membrane was probed with an anti-GAPDH mouse monoclonal antibody followed by an HRP-conjugated goat anti-human IgG F(ab’)2 secondary antibody (Product # 31482) at a dilution of 1:10,000 for 1 hour and DAB substrate (Panel A). Because GAPDH was not detected with Product # 31482, the blot was rinsed with PBS and probed with an anti-mouse IgG-HRP secondary antibody diluted 1:4000 follwed by DAB substrate (Panel B). These data demonstrate reactivity of Product # 31482 with a humanized mouse Fab, but not with intact mouse IgG. Data courtesy of the Innovators Program.
     
  • WB using MA1-10378
    Western Blot with anti-Human IgG Fc Monoclonal Secondary Antibody [EM-07] (MA1-10378)

    Western Blot with anti-Human IgG Fc Monoclonal Secondary Antibody [EM-07] (MA1-10378)

    IgG kappa light chain (1), IgG lamda light chain (2) and IgG Fc fragment (3) purified from human serum were analysed by Western blotting with MEM-09 antibody against IgG kappa light chain (A) and EM-07 antibody against IgG Fc fragment (B).
     
  • ELISA using MA1-82854
    ELISA with anti-Human IgE Monoclonal Secondary Antibody [E411 (5H2)] (MA1-82854)

    ELISA with anti-Human IgE Monoclonal Secondary Antibody [E411 (5H2)] (MA1-82854)

    ELISA analysis with a human IgE monoclonal antibody (Product # MA1-82854)) as the capture antibody, human IgE (RP-86985) as the antigen, and a human IgE monoclonal antibody (Product # MA1-82854) (conjugated to HRP) as the detection antibody
     
  • ELISA using MA5-11208
    ELISA with anti-Human IgA (Alpha heavy chain) Monoclonal Secondary Antibody [GA01] (MA5-11208)

    ELISA with anti-Human IgA (Alpha heavy chain) Monoclonal Secondary Antibody [GA01] (MA5-11208)

    Direct ELISA analysis of IgA was performed by coating wells of a 96-well plate with 100ul per well of Human IgA diluted in carbonate/bicarbonate buffer (Product # 28382), starting at a concentration of 5ug/ml and serially diluting 2-fold to a concentration of 5ng/ml, overnight at 4C. Wells of the plate were washed, blocked with StartingBlock blocking buffer (Product # 37538), and incubated with 100ul per well of a mouse anti-human IgA monoclonal antibody (Product # MA5-11208) at a concentration of 1ug/ml for 1 hour at room temperature. The plate was washed, then incubated with 100ul per well of an HRP-conjugated goat anti-mouse IgG Fc secondary antibody (Product # 31439) at a dilution of 1:8000 for 1 hour at room temperature. Detection was performed using 1-Step Ultra TMB substrate (Product # 34028) for 5 minutes at room temperature in the dark. The reaction was stopped with 0.16M sulfuric acid, and absorbances were read on a spectrophotometer at 450-550nm.
     
  • ELISA using MA5-11208
    ELISA with anti-Human IgA (Alpha heavy chain) Monoclonal Secondary Antibody [GA01] (MA5-11208)

    ELISA with anti-Human IgA (Alpha heavy chain) Monoclonal Secondary Antibody [GA01] (MA5-11208)

    Direct ELISA analysis of various immunoglobulin (Ig) isotypes was performed by coating wells of a 96-well plate with 100ul per well of Human IgA, Human IgG, Human IgM, or Mouse IgA diluted in carbonate/bicarbonate buffer (Product # 28382), starting at a concentration of 300ng/ml and serially diluting 2-fold to a concentration of 0.29ng/ml, overnight at 4C. Wells of the plate were washed, blocked with StartingBlock blocking buffer (Product # 37538), and incubated with 100ul per well of a mouse anti-human IgA monoclonal antibody (Product # MA5-11208) at a concentration of 1ug/ml for 1 hour at room temperature. The plate was washed, then incubated with 100ul per well of an HRP-conjugated goat anti-mouse IgG Fc secondary antibody (Product # 31439) at a dilution of 1:8000 for 1 hour at room temperature. Detection was performed using 1-Step Ultra TMB substrate (Product # 34028) for 5 minutes at room temperature in the dark. The reaction was stopped with 0.16M sulfuric acid, and absorbances were read on a spectrophotometer at 450-550nm.
     
  • FACS using MA5-11208
    Flow Cytometry with anti-Human IgA (Alpha heavy chain) Monoclonal Secondary Antibody [GA01] (MA5-11208)

    Flow Cytometry with anti-Human IgA (Alpha heavy chain) Monoclonal Secondary Antibody [GA01] (MA5-11208)

    Flow cytometry analysis of Human IgA (alpha-Heavy Chain) in Daudi cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a Mouse anti-Human IgA (alpha-Heavy Chain) Secondary antibody (Product # MA5-11208) at a dilution of 2 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.
     
  • FACS using MA5-11208
    Flow Cytometry with anti-Human IgA (Alpha heavy chain) Monoclonal Secondary Antibody [GA01] (MA5-11208)

    Flow Cytometry with anti-Human IgA (Alpha heavy chain) Monoclonal Secondary Antibody [GA01] (MA5-11208)

    Flow cytometry analysis of Human IgA (alpha-Heavy Chain) in Ramos cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a Mouse anti-Human IgA (alpha-Heavy Chain) Secondary antibody (Product # MA5-11208) at a dilution of 2 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.
     
  • IHC using MA5-11208
    Immunohistochemistry with anti-Human IgA (Alpha heavy chain) Monoclonal Secondary Antibody [GA01] (MA5-11208)

    Immunohistochemistry with anti-Human IgA (Alpha heavy chain) Monoclonal Secondary Antibody [GA01] (MA5-11208)

    Formalin-fixed, paraffin-embedded human tonsil stained with IgA antibody using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining of IgA secreting cells.
     
  • WB using MA5-11208
    Western Blot with anti-Human IgA (Alpha heavy chain) Monoclonal Secondary Antibody [GA01] (MA5-11208)

    Western Blot with anti-Human IgA (Alpha heavy chain) Monoclonal Secondary Antibody [GA01] (MA5-11208)

    Western blot analysis of IgA was performed by loading the indicated amounts of purified Human IgA and 10ul of PageRuler Prestained Protein Ladder (Product # 26616) per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane using the G2 Fast Blotter (Product # 62288), and blocked with 5% BSA in TBST for at least 1 hour at room temperature. IgA heavy chain was detected at ~55kD using a mouse anti-human IgA monoclonal antibody (Product # MA5-11208) diluted to a concentration of 0.5ug/ml in blocking buffer, overnight at 4C overnight on a rocking platform. The blot was washed in TBST, and probed with an HRP-conjugated goat anti-mouse light chain secondary antibody at a dilution of 1:40,000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal West Pico.
     
  • WB using MA5-11208
    Western Blot with anti-Human IgA (Alpha heavy chain) Monoclonal Secondary Antibody [GA01] (MA5-11208)

    Western Blot with anti-Human IgA (Alpha heavy chain) Monoclonal Secondary Antibody [GA01] (MA5-11208)

    Western blot analysis of IgA was performed by loading 0.5ug of purified Human IgA, Human IgG, Human IgM, and Mouse IgA, and 10ul of PageRuler Prestained Protein Ladder (Product # 26616) per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to PVDF membranes using the G2 Fast Blotter (Product # 62288), and blocked with 5% BSA in TBST for at least 1 hour at room temperature. IgA heavy chain was detected at ~55kD using a mouse anti-human IgA monoclonal antibody (Product # MA5-11208) diluted to a concentration of 0.5ug/ml in blocking buffer, overnight at 4C on a rocking platform, followed by an HRP-conjugated goat anti-mouse light chain secondary antibody diluted 1:40,000 for at least 30 minutes at room temperature (panel A). To verify the presence of all isotypes, a second blot was probed with a goat anti-human IgM + IgG +IgA polyclonal antibody (Product # 31128) at a concentration of 0.5ug/ml in blocking buffer overnight at 4C on a rocking platform, followed by an HRP-conjugated mouse anti-goat IgG secondary antibody (Product # 31400) diluted 1:40,000 in blocking buffer for at least 30 minutes at room temperature (panel B). Chemiluminescent detection was performed using SuperSignal West Pico.
     
  • WB using MA5-11208
    Western Blot with anti-Human IgA (Alpha heavy chain) Monoclonal Secondary Antibody [GA01] (MA5-11208)

    Western Blot with anti-Human IgA (Alpha heavy chain) Monoclonal Secondary Antibody [GA01] (MA5-11208)

    Western blot analysis of IgA was performed by loading 25ug of various human tissue lysates, 0.5ug of Human IgA, 0.25ul of human serum, and 10ul of PageRuler Prestained Protein Ladder (Product # 26616) per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane using the G2 Fast Blotter (Product # 62288), and blocked with 5% BSA in TBST for at least 1 hour at room temperature. IgA heavy chain was detected at ~55kD using a mouse anti-human IgA monoclonal antibody (Product # MA5-11208) diluted to a concentration of 0.5ug/ml in blocking buffer, overnight at 4C on a rocking platform, followed by an HRP-conjugated goat anti-mouse light chain secondary antibody diluted 1:40,000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal West Pico. *The band at ~25kD is a result of spill over from an adjacent lane.
     
  • WB using MA5-11208
    Western Blot with anti-Human IgA (Alpha heavy chain) Monoclonal Secondary Antibody [GA01] (MA5-11208)

    Western Blot with anti-Human IgA (Alpha heavy chain) Monoclonal Secondary Antibody [GA01] (MA5-11208)

    Western blot analysis of Human IgA (Alpha heavy chain) was performed by loading 25 ug of Daudi cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a Mouse anti-Human IgA (alpha-Heavy Chain) Secondary antibody (Product # MA5-11208) at a dilution of 1:100 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~60kDa.
     
  • WB using MA5-14755
    Western Blot with anti-human IgG Monoclonal Secondary Antibody [4D2D9G8] (MA5-14755)

    Western Blot with anti-human IgG Monoclonal Secondary Antibody [4D2D9G8] (MA5-14755)

    Western blot analysis of human IgG using human IgG monoclonal Secondary antibody (Product # MA5-14755) in human serum (lane 1, 3) and plasma (lane 2, 4).
     
  • FACS using PA5-16388
    Flow Cytometry with anti-Human IgA (Alpha heavy chain) Polyclonal Secondary Antibody (PA5-16388)

    Flow Cytometry with anti-Human IgA (Alpha heavy chain) Polyclonal Secondary Antibody (PA5-16388)

    Flow cytometry analysis of Human IgA (alpha-Heavy Chain) in Ramos cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a Rabbit anti-Human IgA (alpha-Heavy Chain) Secondary antibody (Product # PA5-16388) at a dilution of 1:10 for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.
     
  • IHC using PA5-16388
    Immunohistochemistry with anti-Human IgA (Alpha heavy chain) Polyclonal Secondary Antibody (PA5-16388)

    Immunohistochemistry with anti-Human IgA (Alpha heavy chain) Polyclonal Secondary Antibody (PA5-16388)

    Formalin-fixed, paraffin-embedded human tonsil stained with IgA antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of IgAecreting cells.
     
  • WB using PA5-16388
    Western Blot with anti-Human IgA (Alpha heavy chain) Polyclonal Secondary Antibody (PA5-16388)

    Western Blot with anti-Human IgA (Alpha heavy chain) Polyclonal Secondary Antibody (PA5-16388)

    Western blot analysis of Human IgA was performed by loading 25 ug of Raji (lane 1), Ramos (lane 2) and mouse spleen (lane 3) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a Rabbit anti-Human IgA (alpha-Heavy Chain) secondary antibody (Product # PA5-16388) at a dilution of 1:200 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~50-75 kDa.
     
  • FACS using PA5-16396
    Flow Cytometry with anti-Human IgE (Heavy chain) Polyclonal Secondary Antibody (PA5-16396)

    Flow Cytometry with anti-Human IgE (Heavy chain) Polyclonal Secondary Antibody (PA5-16396)

    Flow cytometry analysis of in Ramos cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a Rabbit anti-Human IgE secondary antibody (Product # PA5-16396) at a dilution of 1:10 for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.
     
  • IHC using PA5-16396
    Immunohistochemistry with anti-Human IgE (Heavy chain) Polyclonal Secondary Antibody (PA5-16396)

    Immunohistochemistry with anti-Human IgE (Heavy chain) Polyclonal Secondary Antibody (PA5-16396)

    Formalin-fixed, paraffin-embedded human tonsil stained with IgE antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of IgEecreting cells.
     
  • IHC using PA5-16400
    Immunohistochemistry with anti-Human IgD (Heavy chain) Polyclonal Secondary Antibody (PA5-16400)

    Immunohistochemistry with anti-Human IgD (Heavy chain) Polyclonal Secondary Antibody (PA5-16400)

    Formalin-fixed, paraffin-embedded human tonsil stained with IgD antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of IgDecreting cells.
     
  • WB using PA5-16400
    Western Blot with anti-Human IgD (Heavy chain) Polyclonal Secondary Antibody (PA5-16400)

    Western Blot with anti-Human IgD (Heavy chain) Polyclonal Secondary Antibody (PA5-16400)

    Western blot analysis of Human IgD was performed by loading 25 ug of Ramos cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a Rabbit anti-Human IgD (delta-Heavy Chain) secondary antibody (Product # PA5-16400) at a dilution of 1:200 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~50-75 kDa.
     
  • WB using SA1-19255
    Western Blot with anti-Human IgG Fab Monoclonal Secondary Antibody [4A11] (SA1-19255)

    Western Blot with anti-Human IgG Fab Monoclonal Secondary Antibody [4A11] (SA1-19255)

    Western Blotting analysis of human IgG Fab fragment using anti-human IgG Fab fragment secondary antibody (SA1-19255). Lane 1: Normal human immunoglobulins Lane 2: Normal bovine immunoglobulins.
     
  • IHC (P) using SA1-19258
    Immunohistochemistry (Paraffin) with anti-Human IgA Monoclonal Secondary Antibody [AD3] (SA1-19258)

    Immunohistochemistry (Paraffin) with anti-Human IgA Monoclonal Secondary Antibody [AD3] (SA1-19258)

    Immunohistochemistry staining of human colon (paraffin sections) with anti-IgA secondary antibody (SA1-19258).
     
  • FACS using SA1-19259
    Flow Cytometry with anti-Human IgE Monoclonal Secondary Antibody [BE5] (SA1-19259)

    Flow Cytometry with anti-Human IgE Monoclonal Secondary Antibody [BE5] (SA1-19259)

  • FACS using SA1-19259
    Flow Cytometry with anti-Human IgE Monoclonal Secondary Antibody [BE5] (SA1-19259)

    Flow Cytometry with anti-Human IgE Monoclonal Secondary Antibody [BE5] (SA1-19259)

  • FACS using SA1-19468
    Flow Cytometry with anti-Human IgM Fc Monoclonal Secondary Antibody [CH2], APC conjugate (SA1-19468)

    Flow Cytometry with anti-Human IgM Fc Monoclonal Secondary Antibody [CH2], APC conjugate (SA1-19468)

    Flow Cytometric surface staining of human peripheral blood cells with anti-human IgM monoclonal (SA1-19468) APC. Cells in the lymphocyte gate were used for analysis.
     
  • FACS using SA1-19599
    Flow Cytometry with anti-Human IgE Monoclonal Secondary Antibody [BE5], FITC conjugate (SA1-19599)

    Flow Cytometry with anti-Human IgE Monoclonal Secondary Antibody [BE5], FITC conjugate (SA1-19599)

  • FACS using SA1-19599
    Flow Cytometry with anti-Human IgE Monoclonal Secondary Antibody [BE5], FITC conjugate (SA1-19599)

    Flow Cytometry with anti-Human IgE Monoclonal Secondary Antibody [BE5], FITC conjugate (SA1-19599)

 
 

PubMed References
ELISA
  Species / Dilution Summary
  Hu / Not Cited
31125 was used in ELISA to develop a strategy to tangle the hepatitis C virus hypervariability:

EMBO J. 1998 Jul 1;17(13):3521-33.
"Towards a solution for hepatitis C virus hypervariability: mimotopes of the hypervariable region 1 can induce antibodies cross-reacting with a large number of viral variants."
Author(s): Puntoriero G, Meola A, Lahm A, Zucchelli S, Ercole BB, Tafi R, Pezzanera M, Mondelli MU, Cortese R, Tramontano A, Galfre' G, Nicosia A
Number of Citations: 1
(See PubMed article )
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