His-Tag Antibodies

The His-tag is a series of six to nine histadine residues generally fused to either the carboxy or amino terminus of the targeted protein in transfected cells.  The small size of the His-tag, even compared with other common epitope tags, makes it less likely to obstruct the target protein’s structure or function and more suitable to use under denaturing conditions. The string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions, allowing for the simple purification and detection of His-tagged proteins. His-Tag antibodies provide another dependable method for the detection and purification of tagged target proteins without a protein-specific antibody or probe. Pierce His-Tag antibodies reliably detect recombinant His-tagged proteins, and each antibody is validated for use in many applications.

Related Links:  
Epitope Tag Control Antibodies
His-Tagged Proteins
His-Tagged Protein Purification Resins and Kits
 

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Antibody (10)
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Product Description Host Target Species Applications Imgs / Refs Size Product # Price
6x-His Epitope Tag Antibody (HIS.H8) Mouse N/A, Tag WB, IF, IP, ELISA 59 100 µg MA1-21315 $149.00
6x-His Epitope Tag Antibody (HIS.H8) Mouse Tag WB, IF, IP, ELISA 40 1 mg MA1-21315-1MG $899.00
6x-His Epitope Tag Antibody, Biotin conjugate (HIS.H8) Mouse Tag WB, IF, IP, ELISA 50 50 µL MA1-21315-BTIN $149.00
6X-His Epitope Tag Antibody, DyLight 488 conjugate (HIS.H8) Mouse Tag IF 10 50 µL MA1-21315-D488 $149.00
6x-His Epitope Tag Antibody, DyLight 550 conjugate (HIS.H8) Mouse Tag IF 10 50 µL MA1-21315-D550 $149.00
6x-His Epitope Tag Antibody, DyLight 650 conjugate (HIS.H8) Mouse Tag IF 10 50 µL MA1-21315-D650 $149.00
6x-His Epitope Tag Antibody, DyLight 680 conjugate (HIS.H8) Mouse Tag WB, IF 20 50 µL MA1-21315-D680 $149.00
6x-His Epitope Tag Antibody Rabbit Tag WB 10 200 µg PA1-983B $230.00
6x-His Epitope Tag Antibody Chicken Tag WB, ICC, ELISA 00 200 ug PA1-9531 $255.00
6x-His Epitope Tag Antibody, HRP conjugate Goat Tag WB, IF, ICC, ELISA 00 100 µl PA1-23024 $525.00
Product Images
Filter :
  • IF using MA1-21315
    Immunofluorescence with anti-6x-His Epitope Tag Monoclonal Antibody [HIS.H8] (MA1-21315)

    Immunofluorescence with anti-6x-His Epitope Tag Monoclonal Antibody [HIS.H8] (MA1-21315)

    LEFT: untransfected control; RIGHT: anti-His (in red) on His-tagged fusion proteins in HEK293 cells. Both counterstained with DAPI (in blue)
     
  • WB using MA1-21315
    Western Blot with anti-6x-His Epitope Tag Monoclonal Antibody [HIS.H8] (MA1-21315)

    Western Blot with anti-6x-His Epitope Tag Monoclonal Antibody [HIS.H8] (MA1-21315)

    Western blot analysis of recombinant Treponema denticola major outer sheath His-tagged protein fragments (rMSP, rMSP-C, rMSP-V, rMSP-N) purified from E. coli was performed by loading 5ug of per well onto an SDS-PAGE gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5% milk in TBST for 1 hour. The membrane was probed with a 6X-His Epitope Tag Antibody (Product # MA1-21315) at a dilution of 1:2000 overnight at 4C, washed in TBST, and probed with an HRP-conjugated donkey anti-mouse IgG secondary antibody at a dilution of 1:15,000 for 1 hour at room temperature. Detection was performed using a chemiluminescent substrate. Data courtesy of the Innovators Program.
     
  • WB using MA1-21315
    Western Blot with anti-6x-His Epitope Tag Monoclonal Antibody [HIS.H8] (MA1-21315)

    Western Blot with anti-6x-His Epitope Tag Monoclonal Antibody [HIS.H8] (MA1-21315)

    Comparison between anti-His tag (HIS.H8 / EH158) mAb with 2 different vendor Abs, probed against a standard ladder containing five different His-tagged proteins. All Ab dilutions are 1:2000 (0.5ug/mL)
     
  • WB using MA1-21315
    Western Blot with anti-6x-His Epitope Tag Monoclonal Antibody [HIS.H8] (MA1-21315)

    Western Blot with anti-6x-His Epitope Tag Monoclonal Antibody [HIS.H8] (MA1-21315)

    Standard ladder containing five different His-tagged proteins; untransfected control (1), HEK293 cells transfected with His-tagged protein vector (2)
     
  • WB using MA1-21315
    Western Blot with anti-6x-His Epitope Tag Monoclonal Antibody [HIS.H8] (MA1-21315)

    Western Blot with anti-6x-His Epitope Tag Monoclonal Antibody [HIS.H8] (MA1-21315)

    Western blot analysis of His-tagged protein in E. coli lysates. Membranes containing total cell lysates from uninduced E. coli (-) or induced E. coli (+) treated with 1mM Isopropyl beta-D-1-thiogalactopyranoside (IPTG) to induce expression of His-tagged IQ domain (IQD) protein were blocked with 5% non-fat milk in PBS-T and probed with a 6X-His Epitope Tag Antibody (Product # MA1-21315) at a dilution of 1:1400 followed by a donkey anti-mouse IgG-HRP secondary antibody. Blots were developed with SuperSignal West Dura (Product # 34076). His-tagged Repressor of Gibberellin (RGA) was used as a positive control. Data courtesy of the Innovators Program.
     
  • IF using MA1-21315-1MG
    Immunofluorescence with anti-6x-His Epitope Tag Monoclonal Antibody [HIS.H8] (MA1-21315-1MG)

    Immunofluorescence with anti-6x-His Epitope Tag Monoclonal Antibody [HIS.H8] (MA1-21315-1MG)

    LEFT: untransfected control; RIGHT: anti-His (in red) on His-tagged fusion proteins in HEK293 cells. Both counterstained with DAPI (in blue)
     
  • WB using MA1-21315-1MG
    Western Blot with anti-6x-His Epitope Tag Monoclonal Antibody [HIS.H8] (MA1-21315-1MG)

    Western Blot with anti-6x-His Epitope Tag Monoclonal Antibody [HIS.H8] (MA1-21315-1MG)

    Standard ladder containing five different His-tagged proteins; untransfected control (1), HEK293 cells transfected with His-tagged protein vector (2)
     
  • WB using MA1-21315-1MG
    Western Blot with anti-6x-His Epitope Tag Monoclonal Antibody [HIS.H8] (MA1-21315-1MG)

    Western Blot with anti-6x-His Epitope Tag Monoclonal Antibody [HIS.H8] (MA1-21315-1MG)

    Comparison between anti-His tag (HIS.H8 / EH158) mAb with 2 different vendor Abs, probed against a standard ladder containing five different His-tagged proteins. All Ab dilutions are 1:2000 (0.5ug/mL)
     
  • WB using MA1-21315-1MG
    Western Blot with anti-6x-His Epitope Tag Monoclonal Antibody [HIS.H8] (MA1-21315-1MG)

    Western Blot with anti-6x-His Epitope Tag Monoclonal Antibody [HIS.H8] (MA1-21315-1MG)

    Western blot analysis of His-tagged protein in E. coli lysates. Membranes containing total cell lysates from uninduced E. coli (-) or induced E. coli (+) treated with 1mM Isopropyl beta-D-1-thiogalactopyranoside (IPTG) to induce expression of His-tagged IQ domain (IQD) protein were blocked with 5% non-fat milk in PBS-T and probed with a 6X-His Epitope Tag Antibody (Product # MA1-21315) at a dilution of 1:1400 followed by a donkey anti-mouse IgG-HRP secondary antibody. Blots were developed with SuperSignal West Dura (Product # 34076). His-tagged Repressor of Gibberellin (RGA) was used as a positive control. Data courtesy of the Innovators Program.
     
  • IF using MA1-21315-BTIN
    Immunofluorescence with anti-6x-His Epitope Tag Monoclonal Antibody [HIS.H8], Biotin conjugate (MA1-21315-BTIN)

    Immunofluorescence with anti-6x-His Epitope Tag Monoclonal Antibody [HIS.H8], Biotin conjugate (MA1-21315-BTIN)

    LEFT: untransfected control; RIGHT: anti-His (in red) on His-tagged fusion proteins in HEK293 cells. Both counterstained with DAPI (in blue).
     
  • WB using MA1-21315-BTIN
    Western Blot with anti-6x-His Epitope Tag Monoclonal Antibody [HIS.H8], Biotin conjugate (MA1-21315-BTIN)

    Western Blot with anti-6x-His Epitope Tag Monoclonal Antibody [HIS.H8], Biotin conjugate (MA1-21315-BTIN)

    Western blot analysis of 6x-His Epitope Tag was performed by loading various amounts of E. coli lysate containing a multi-epitope tagged protein per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a biotinylated 6x-His Epitope Tag monoclonal antibody (Product # MA1-21315-BTIN) at a dilution of 1:1000 for 1 hour at room temperature, followed by Streptavidin-HRP (Product # 21126) at a dilution of 1:20,000 for 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34078).
     
  • WB using MA1-21315-BTIN
    Western Blot with anti-6x-His Epitope Tag Monoclonal Antibody [HIS.H8], Biotin conjugate (MA1-21315-BTIN)

    Western Blot with anti-6x-His Epitope Tag Monoclonal Antibody [HIS.H8], Biotin conjugate (MA1-21315-BTIN)

    Comparison between anti-His tag (HIS.H8 / EH158) mAb with 2 different vendor Abs, probed against a standard ladder containing five different His-tagged proteins. All Ab dilutions are 1:2000 (0.5ug/mL).
     
  • WB using MA1-21315-BTIN
    Western Blot with anti-6x-His Epitope Tag Monoclonal Antibody [HIS.H8], Biotin conjugate (MA1-21315-BTIN)

    Western Blot with anti-6x-His Epitope Tag Monoclonal Antibody [HIS.H8], Biotin conjugate (MA1-21315-BTIN)

    Standard ladder containing five different His-tagged proteins; untransfected control (1), HEK293 cells transfected with His-tagged protein vector (2).
     
  • WB using MA1-21315-BTIN
    Western Blot with anti-6x-His Epitope Tag Monoclonal Antibody [HIS.H8], Biotin conjugate (MA1-21315-BTIN)

    Western Blot with anti-6x-His Epitope Tag Monoclonal Antibody [HIS.H8], Biotin conjugate (MA1-21315-BTIN)

    Western blot analysis of His-tagged protein in E. coli lysates. Membranes containing total cell lysates from uninduced E. coli (-) or induced E. coli (+) treated with 1mM Isopropyl beta-D-1-thiogalactopyranoside (IPTG) to induce expression of His-tagged IQ domain (IQD) protein were blocked with 5% non-fat milk in PBS-T and probed with a 6X-His Epitope Tag Antibody (Product # MA1-21315) at a dilution of 1:1400 followed by a donkey anti-mouse IgG-HRP secondary antibody. Blots were developed with SuperSignal West Dura (Product # 34076). His-tagged Repressor of Gibberellin (RGA) was used as a positive control. Data courtesy of the Innovators Program.
     
  • IF using MA1-21315-D488
    Immunofluorescence with anti-6X-His Epitope Tag Monoclonal Antibody [HIS.H8], DyLight 488 conjugate (MA1-21315-D488)

    Immunofluorescence with anti-6X-His Epitope Tag Monoclonal Antibody [HIS.H8], DyLight 488 conjugate (MA1-21315-D488)

    Immunofluorescent analysis of HeLa cells transfected with a construct containing a 6x-His Epitope Tag. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a DyLight 488-conjugated 6x-His Epitope Tag monoclonal antibody (Product # MA1-21315-D488) at a dilution of 1:25 for at least 1 hour at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan or ToxInsight Instrument at 20X magnification.
     
  • IF using MA1-21315-D550
    Immunofluorescence with anti-6x-His Epitope Tag Monoclonal Antibody [HIS.H8], DyLight 550 conjugate (MA1-21315-D550)

    Immunofluorescence with anti-6x-His Epitope Tag Monoclonal Antibody [HIS.H8], DyLight 550 conjugate (MA1-21315-D550)

    Immunofluorescent analysis of HeLa cells transfected with a construct containing a 6x-His Epitope Tag. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a DyLight 550-conjugated 6x-His Epitope Tag monoclonal antibody (Product # MA1-21315-D550) at a dilution of 1:25 for at least 1 hour at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan or ToxInsight Instrument at 20X magnification.
     
  • IF using MA1-21315-D650
    Immunofluorescence with anti-6x-His Epitope Tag Monoclonal Antibody [HIS.H8], DyLight 650 conjugate (MA1-21315-D650)

    Immunofluorescence with anti-6x-His Epitope Tag Monoclonal Antibody [HIS.H8], DyLight 650 conjugate (MA1-21315-D650)

    Immunofluorescent analysis of HeLa cells transfected with a construct containing a 6x-His Epitope Tag. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a DyLight 650-conjugated 6x-His Epitope Tag monoclonal antibody (Product # MA1-21315-D650) at a dilution of 1:25 for at least 1 hour at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan or ToxInsight Instrument at 20X magnification.
     
  • IF using MA1-21315-D680
    Immunofluorescence with anti-6x-His Epitope Tag Monoclonal Antibody [HIS.H8], DyLight 680 conjugate (MA1-21315-D680)

    Immunofluorescence with anti-6x-His Epitope Tag Monoclonal Antibody [HIS.H8], DyLight 680 conjugate (MA1-21315-D680)

    Immunofluorescent analysis of HeLa cells transfected with a construct containing a 6x-His Epitope Tag. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a DyLight 680-conjugated 6x-His Epitope Tag monoclonal antibody (Product # MA1-21315-D680) at a dilution of 1:25 for at least 1 hour at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan or ToxInsight Instrument at 20X magnification.
     
  • WB using MA1-21315-D680
    Western Blot with anti-6x-His Epitope Tag Monoclonal Antibody [HIS.H8], DyLight 680 conjugate (MA1-21315-D680)

    Western Blot with anti-6x-His Epitope Tag Monoclonal Antibody [HIS.H8], DyLight 680 conjugate (MA1-21315-D680)

    Western blot analysis of 6x-His Epitope Tag was performed by loading various amounts of E. coli lysate containing a multi-epitope tagged protein per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a DyLight 680-conjugated 6x-His Epitope Tag monoclonal antibody (Product # MA1-21315-D680) at a dilution of 1:1000 for 1 hour at room temperature on a rocking platform and washed in TBS-0.1% Tween-20. Detection was performed using the LI-COR Odyssey.
     
  • WB using PA1-983B
    Western Blot with anti-6x-His Epitope Tag Polyclonal Antibody (PA1-983B)

    Western Blot with anti-6x-His Epitope Tag Polyclonal Antibody (PA1-983B)

    Western blot analysis of His-tagged GFP was performed by loading 100ng of purified recombinant His-GFP and 7ul of Molecular Weight Protein Ladder per well onto a 4-12% Bis-Tris polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with an anti-6X-His polyclonal antibody (Product # PA1983B) at a dilution of 1:250 overnight at 4°C on a rocking platform, washed in TBS-0.1%Tween-20, and probed with a goat anti-rabbit IgG-HRP secondary antibody (Product # 32460) at a dilution of 1:150,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075).
     
 
 

PubMed References
Western Blot
  Species / Dilution Summary
  N/A / 1:500
MA1-21315 was used in western blot to study the P2X receptor ion channel ion access pathway

J Gen Physiol. 2011 Jun;137(6):579-90.
"Ion access pathway to the transmembrane pore in P2X receptor channels."
Author(s): Kawate T, Robertson JL, Li M, Silberberg SD, Swartz KJ
Number of Citations: 19
(See PubMed article )
  N/A / 1:2500
MA1-21315 was used in western blot to study the ability of Escherchia coli YmdB to regulate biofilm formation independently of ribonuclease III

BMC Microbiol. 2013 Nov 24;13():266.
"Escherichia coli YmdB regulates biofilm formation independently of its role as an RNase III modulator."
Author(s): Kim T, Lee J, Kim KS
Number of Citations: 0
(See PubMed article )
  N/A / 1:1000
MA1-21315 was used in western blot to characterize SUMO cycle components in Hydra

Genesis. 2013 Sep;51(9):619-29.
"The Hydra small ubiquitin-like modifier."
Author(s): Khan U, Mehere P, Deivasigamani S, Ratnaparkhi GS
Number of Citations: 0
(See PubMed article )
  N/A / 0
MA1-21315 was used in western blot to express and purify the nucleocapsid protein of Schmallenberg virus, and raise a monclonal antibody against it

Protein Expr Purif. 2013 Nov;92(1):1-8.
"Expression and purification of the nucleocapsid protein of Schmallenberg virus, and preparation and characterization of a monoclonal antibody against this protein."
Author(s): Zhang Y, Wu S, Wang J, Wernike K, Lv J, Feng C, Zhang J, Wang C, Deng J, Yuan X, Lin X
Number of Citations: 0
(See PubMed article )
  N/A / 0
MA1-21315 was used in western blot to establish a process for producing HIV-1 capsid protein p24 in E.coli

BMC Biotechnol. 2013 Dec 4;13():107.
"Production and purification of polymerization-competent HIV-1 capsid protein p24 (CA) in NiCo21(DE3) Escherichia coli."
Author(s): Teow SY, Mualif SA, Omar TC, Wei CY, Yusoff NM, Ali SA
Number of Citations: 0
(See PubMed article )
  N/A / 0
MA1-21315 was used in western blot to study whether the recombinant C-terminal olfactomedin domain of myocilin affects outflow facility in porcine eyes

Invest Ophthalmol Vis Sci. 2003 May;44(5):1953-61.
"Perfusion with the olfactomedin domain of myocilin does not affect outflow facility."
Author(s): Goldwich A, Ethier CR, Chan DW, Tamm ER
Number of Citations: 8
(See PubMed article )
  N/A / 0
MA1-21315 was used in western blot to study Mcm10 homocomplex assembly

J Biol Chem. 2003 Sep 19;278(38):36051-8.
"A novel zinc finger is required for Mcm10 homocomplex assembly."
Author(s): Cook CR, Kung G, Peterson FC, Volkman BF, Lei M
Number of Citations: 13
(See PubMed article )
  N/A / 1:1000
MA1-21315 was used in western blot to study the interaction between SUMO and vestigial and its role in fly wing development

Mech Dev. 2005 Oct;122(10):1130-7.
"SUMO enhances vestigial function during wing morphogenesis."
Author(s): Takanaka Y, Courey AJ
Number of Citations: 3
(See PubMed article )
  N/A / 0
MA1-21315 was used in western blot to biochemically characterize diacylglycerol kinase AtDGK7 of Arabadopsis thaliana

J Biol Chem. 2005 Oct 14;280(41):34888-99.
"Arabidopsis AtDGK7, the smallest member of plant diacylglycerol kinases (DGKs), displays unique biochemical features and saturates at low substrate concentration: the DGK inhibitor R59022 differentially affects AtDGK2 and AtDGK7 activity in vitro and alters plant growth and development."
Author(s): Gómez-Merino FC, Arana-Ceballos FA, Trejo-Téllez LI, Skirycz A, Brearley CA, Dörmann P, Mueller-Roeber B
Number of Citations: 15
(See PubMed article )
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