DyLight Fluor Secondary Antibodies and Controls

Thermo Scientific DyLight Fluor Secondary Antibodies are high-performance fluorescent conjugates for use as secondary molecular probes in fluorescence microscopy, flow cytometry, Western blotting, ELISA, high-content screening and other array platforms. DyLight Fluor Conjugated Antibodies are especially useful for multiplex experiments in which primary antibodies from two or more host species are used simultaneously. They are designed to reduce nonspecific background staining and offer high specificity, low background and increased assay sensitivity.

Thermo Scientific Pierce secondary antibodies are available as whole IgG or the F(ab')2 fragment. Each of these antibodies is affinity purified using its target antigen (the primary antibody class, subclass, and fragment) coupled to an agarose support. This affinity purification eliminates nonspecific antibodies, resulting in high specificity and low background signal. Conjugated antibodies are affinity purified before labeling. Selected secondary antibodies have been further purified by adsorbing the antigen-purified antibody to an affinity column containing immobilized serum proteins of selected species. This additional processing minimizes antibody cross-reactivity to the serum proteins of those selected species. These antibodies are designated as Cross Adsorbed.

Related Links:
DyLight Conjugated Phalloidins
DAPI Nuclear Counterstain
Hoechst 33342 Fluorescent Stain
DRAQ5 Fluoescent Probe
Whole Cell Fluorescent Stains

Use the filter search on the left to find your desired DyLight-conjugated secondary antibody by host, DyLight fluor conjugate, class, type, target species, clone, or application.
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Secondary Antibody (176)
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[Ms] Mouse (41)
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Product Description Host Target Species Applications Imgs / Refs Size Product # Price
Goat anti-Mouse IgG (H+L) Secondary Antibody, DyLight 488 conjugate Goat Ms WB, IF, ICC, IHC, FACS, IP 00 1 ml 35502
Goat anti-Mouse IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 488 conjugate Goat Ms WB, IF, ICC, IHC, FACS, IP 00 500 ul 35503
Goat anti-Mouse IgG (H+L) Secondary Antibody, DyLight 594 conjugate Goat Ms WB, IF, ICC, IHC, FACS, IP 00 1 ml 35510
Goat anti-Mouse IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 594 conjugate Goat Ms WB, IF, ICC, IHC, FACS, IP 00 500 ul 35511
Goat anti-Mouse IgG (H+L) Secondary Antibody, DyLight 633 conjugate Goat Ms WB, IF, ICC, IHC, FACS, IP 00 1 ml 35512
Goat anti-Mouse IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 633 conjugate Goat Ms WB, IF, ICC, IHC, FACS, IP 00 500 ul 35513
Goat anti-Mouse IgG (H+L) Secondary Antibody, DyLight 680 conjugate Goat Ms WB, IF, ICC, IHC, FACS, IP 00 1 ml 35518
Goat anti-Mouse IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 680 conjugate Goat Ms WB, IF, ICC, IHC, FACS, IP 00 500 ul 35519
Goat anti-Rabbit IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 405 conjugate Goat Rb WB, IF, ICC, IHC, FACS, IP 00 500 ul 35550
Goat anti-Rabbit IgG (H+L) Secondary Antibody, DyLight 405 conjugate Goat Rb WB, IF, ICC, IHC, FACS, IP 00 1 ml 35551
Goat anti-Rabbit IgG (H+L) Secondary Antibody, DyLight 488 conjugate Goat Rt, Rb WB, IF, ICC, IHC, FACS, IP 01 1 ml 35552
Goat anti-Rabbit IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 488 conjugate Goat Rb WB, IF, ICC, IHC, FACS, IP 00 500 ul 35553
Goat anti-Rabbit IgG (H+L) Secondary Antibody, DyLight 594 conjugate Goat Rb WB, IF, ICC, IHC, FACS, IP 00 1 ml 35560
Goat anti-Rabbit IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 594 conjugate Goat Rb WB, IF, ICC, IHC, FACS, IP 00 500 ul 35561
Goat anti-Rabbit IgG (H+L) Secondary Antibody, DyLight 633 conjugate Goat Rb WB, IF, ICC, IHC, FACS, IP 00 1 ml 35562
Goat anti-Rabbit IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 633 conjugate Goat Rb WB, IF, ICC, IHC, FACS, IP 00 500 ul 35563
Goat anti-Rabbit IgG (H+L) Secondary Antibody, DyLight 680 conjugate Goat Rb WB, IF, ICC, IHC, FACS, IP 00 1 ml 35568
Goat anti-Rabbit IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 680 conjugate Goat Rb WB, IF, ICC, IHC, FACS, IP 00 500 ul 35569
Goat anti-Rabbit IgG (H+L) Secondary Antibody, DyLight 350 conjugate Goat Rb WB, IF, ICC, IHC, FACS, IP 00 1 ml 62270
Goat anti-Mouse IgG (H+L) Secondary Antibody, DyLight 350 conjugate Goat Ms WB, IF, ICC, IHC, FACS, IP 00 1 ml 62271
Goat anti-Rabbit IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 350 conjugate Goat Rb WB, IF, ICC, IHC, FACS, IP 00 500 ul 62272
Goat anti-Mouse IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 350 conjugate Goat Ms WB, IF, ICC, IHC, FACS, IP 00 500 ul 62273
Goat anti-Mouse IgG (H+L) Secondary Antibody, DyLight 550 conjugate Goat Ms WB, IF, ICC, IHC, FACS, IP 00 1 ml 84540
Goat anti-Rabbit IgG (H+L) Secondary Antibody, DyLight 550 conjugate Goat Rb WB, IF, ICC, IHC, FACS, IP 00 1 ml 84541
Streptavidin Protein, DyLight 550 conjugate IF 00 1 ml 84542
Goat anti-Mouse IgG (H+L) Secondary Antibody, DyLight 650 conjugate Goat Ms WB, IF, ICC, IHC, FACS, IP 00 1 ml 84545
Goat anti-Rabbit IgG (H+L) Secondary Antibody, DyLight 650 conjugate Goat Rb WB, IF, ICC, IHC, FACS, IP 00 1 ml 84546
Streptavidin Protein, DyLight 650 conjugate IF 00 1 ml 84547
NeutrAvidin Protein, DyLight 550 conjugate IF 00 1 ml 84606
NeutrAvidin Protein, DyLight 650 conjugate IF 00 1 ml 84607
Goat anti-Rat IgM Cross Adsorbed Secondary Antibody, DyLight 350 conjugate Goat Rt WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10009
Goat anti-Rat IgM Cross Adsorbed Secondary Antibody, DyLight 488 conjugate Goat Rt WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10010
Goat anti-Rat IgM Cross Adsorbed Secondary Antibody, DyLight 550 conjugate Goat Rt WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10011
Goat anti-Rat IgM Cross Adsorbed Secondary Antibody, DyLight 594 conjugate Goat Rt WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10012
Goat anti-Rat IgM Cross Adsorbed Secondary Antibody, DyLight 650 conjugate Goat Rt WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10013
Goat anti-Rat IgM Cross Adsorbed Secondary Antibody, DyLight 680 conjugate Goat Rt WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10014
Goat anti-Rat IgM Cross Adsorbed Secondary Antibody, DyLight 755 conjugate Goat Rt WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10015
Goat anti-Rat IgM Cross Adsorbed Secondary Antibody, DyLight 800 conjugate Goat Rt WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10016
Goat anti-Rat IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 350 conjugate Goat Rt WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10017
Goat anti-Rat IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 488 conjugate Goat Rt WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10018
Goat anti-Rat IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 550 conjugate Goat Rt WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10019
Goat anti-Rat IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 594 conjugate Goat Rt WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10020
Goat anti-Rat IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 650 conjugate Goat Rt WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10021
Goat anti-Rat IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 680 conjugate Goat Rt WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10022
Goat anti-Rat IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 755 conjugate Goat Rt WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10023
Goat anti-Rat IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 800 conjugate Goat Rt WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10024
Donkey anti-Rat IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 350 conjugate Donkey Rt WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10025
Donkey anti-Rat IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 488 conjugate Donkey Rt WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10026
Donkey anti-Rat IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 550 conjugate Donkey Rt WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10027
Donkey anti-Rat IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 594 conjugate Donkey Rt WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10028
Donkey anti-Rat IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 650 conjugate Donkey Rt WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10029
Donkey anti-Rat IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 680 conjugate Donkey Rt WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10030
Donkey anti-Rat IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 755 conjugate Donkey Rt WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10031
Donkey anti-Rat IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 800 conjugate Donkey Rt WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10032
Goat anti-Rabbit IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 550 conjugate Goat Rb WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10033
Goat anti-Rabbit IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 650 conjugate Goat Rb WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10034
Goat anti-Rabbit IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 755 conjugate Goat Rb WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10035
Goat anti-Rabbit IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 800 conjugate Goat Rb WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10036
Donkey anti-Rabbit IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 350 conjugate Donkey Rb WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10037
Donkey anti-Rabbit IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 488 conjugate Donkey Rb WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10038
Donkey anti-Rabbit IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 550 conjugate Donkey Rb WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10039
Donkey anti-Rabbit IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 594 conjugate Donkey Rb WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10040
Donkey anti-Rabbit IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 650 conjugate Donkey Rb WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10041
Donkey anti-Rabbit IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 680 conjugate Donkey Rb WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10042
Donkey anti-Rabbit IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 755 conjugate Donkey Rb WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10043
Donkey anti-Rabbit IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 800 conjugate Donkey Rb WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10044
Rabbit anti-Sheep IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 350 conjugate Rabbit Ov WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10053
Rabbit anti-Sheep IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 488 conjugate Rabbit Ov WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10054
Rabbit anti-Sheep IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 550 conjugate Rabbit Ov WB, IF, ICC, IHC, FACS, IP 00 500 µg SA5-10055
Rabbit anti-Sheep IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 594 conjugate Rabbit Ov WB, IF, ICC, IHC, FACS, IP 00 500 µg SA5-10056
Rabbit anti-Sheep IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 650 conjugate Rabbit Ov WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10057
Rabbit anti-Sheep IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 680 conjugate Rabbit Ov WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10058
Rabbit anti-Sheep IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 755 conjugate Rabbit Ov WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10059
Rabbit anti-Sheep IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 800 conjugate Rabbit Ov WB, IF, ICC, IHC, FACS, IP 00 500 µg SA5-10060
Rabbit anti-Donkey IgG (H+L) Secondary Antibody, DyLight 350 conjugate Rabbit Do WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10061
Rabbit anti-Donkey IgG (H+L) Secondary Antibody, DyLight 488 conjugate Rabbit Do WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10062
Rabbit anti-Donkey IgG (H+L) Secondary Antibody, DyLight 550 conjugate Rabbit Do WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10063
Rabbit anti-Donkey IgG (H+L) Secondary Antibody, DyLight 594 conjugate Rabbit Do WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10064
Rabbit anti-Donkey IgG (H+L) Secondary Antibody, DyLight 650 conjugate Rabbit Do WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10065
Rabbit anti-Donkey IgG (H+L) Secondary Antibody, DyLight 680 conjugate Rabbit Do WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10066
Rabbit anti-Donkey IgG (H+L) Secondary Antibody, DyLight 755 conjugate Rabbit Do WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10067
Rabbit anti-Donkey IgG (H+L) Secondary Antibody, DyLight 800 conjugate Rabbit Do WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10068
Goat anti-Chicken IgY (H+L) Cross Adsorbed Secondary Antibody, DyLight 350 conjugate Goat Ck WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10069
Goat anti-Chicken IgY (H+L) Cross Adsorbed Secondary Antibody, DyLight 488 conjugate Goat Ck WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10070
Goat anti-Chicken IgY (H+L) Cross Adsorbed Secondary Antibody, DyLight 550 conjugate Goat Ck WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10071
Goat anti-Chicken IgY (H+L) Cross Adsorbed Secondary Antibody, DyLight 594 conjugate Goat Ck WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10072
Goat anti-Chicken IgY (H+L) Cross Adsorbed Secondary Antibody, DyLight 650 conjugate Goat Ck WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10073
Goat anti-Chicken IgY (H+L) Cross Adsorbed Secondary Antibody, DyLight 680 conjugate Goat Ck WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10074
Goat anti-Chicken IgY (H+L) Cross Adsorbed Secondary Antibody, DyLight 755 conjugate Goat Ck WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10075
Goat anti-Chicken IgY (H+L) Cross Adsorbed Secondary Antibody, DyLight 800 conjugate Goat Ck WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10076
Rabbit anti-Goat IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 350 conjugate Rabbit Gt WB, IF, ICC, IHC, FACS, IP 00 500 µg SA5-10077
Rabbit anti-Goat IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 488 conjugate Rabbit Gt WB, IF, ICC, IHC, FACS, IP 00 500 µg SA5-10078
Rabbit anti-Goat IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 550 conjugate Rabbit Gt WB, IF, ICC, IHC, FACS, IP 00 500 µg SA5-10079
Rabbit anti-Goat IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 594 conjugate Rabbit Gt WB, IF, ICC, IHC, FACS, IP 00 500 µg SA5-10080
Rabbit anti-Goat IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 650 conjugate Rabbit Gt WB, IF, ICC, IHC, FACS, IP 00 500 µg SA5-10081
Rabbit anti-Goat IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 680 conjugate Rabbit Gt WB, IF, ICC, IHC, FACS, IP 00 500 µg SA5-10082
Rabbit anti-Goat IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 755 conjugate Rabbit Gt WB, IF, ICC, IHC, FACS, IP 00 500 µg SA5-10083
Rabbit anti-Goat IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 800 conjugate Rabbit Gt WB, IF, ICC, IHC, FACS, IP 00 500 µg SA5-10084
Donkey anti-Goat IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 350 conjugate Donkey Gt WB, IF, ICC, IHC, FACS, IP 00 500 µg SA5-10085
Donkey anti-Goat IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 488 conjugate Donkey Gt WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10086
Donkey anti-Goat IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 550 conjugate Donkey Gt WB, IF, ICC, IHC, FACS, IP 00 500 µg SA5-10087
Donkey anti-Goat IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 594 conjugate Donkey Gt WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10088
Donkey anti-Goat IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 650 conjugate Donkey Gt WB, IF, ICC, IHC, FACS, IP 00 500 µg SA5-10089
Donkey anti-Goat IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 680 conjugate Donkey Gt WB, IF, ICC, IHC, FACS 00 500 µg SA5-10090
Donkey anti-Goat IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 755 conjugate Donkey Gt WB, IF, ICC, IHC, FACS, IP 00 500 µg SA5-10091
Donkey anti-Goat IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 800 conjugate Donkey Gt WB, IF, ICC, IHC, FACS, IP 00 500 µg SA5-10092
Goat anti-Guinea Pig IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 350 conjugate Goat GP WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10093
Goat anti-Guinea Pig IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 488 conjugate Goat GP WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10094
Goat anti-Guinea Pig IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 550 conjugate Goat GP WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10095
Goat anti-Guinea Pig IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 594 conjugate Goat GP WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10096
Goat anti-Guinea Pig IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 650 conjugate Goat GP WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10097
Goat anti-Guinea Pig IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 680 conjugate Goat GP WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10098
Goat anti-Guinea Pig IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 755 conjugate Goat GP WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10099
Goat anti-Guinea Pig IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 800 conjugate Goat GP WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10100
Goat anti-Human IgM Cross Adsorbed Secondary Antibody, DyLight 350 conjugate Goat Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10101
Goat anti-Human IgM Cross Adsorbed Secondary Antibody, DyLight 488 conjugate Goat Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10102
Goat anti-Human IgM Cross Adsorbed Secondary Antibody, DyLight 550 conjugate Goat Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10103
Goat anti-Human IgM Cross Adsorbed Secondary Antibody, DyLight 594 conjugate Goat Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10104
Goat anti-Human IgM Cross Adsorbed Secondary Antibody, DyLight 650 conjugate Goat Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10105
Goat anti-Human IgM Cross Adsorbed Secondary Antibody, DyLight 680 conjugate Goat Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10106
Goat anti-Human IgM Cross Adsorbed Secondary Antibody, DyLight 755 conjugate Goat Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10107
Goat anti-Human IgM Cross Adsorbed Secondary Antibody, DyLight 800 conjugate Goat Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10108
Rabbit anti-Human IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 350 conjugate Rabbit Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10109
Rabbit anti-Human IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 488 conjugate Rabbit Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10110
Rabbit anti-Human IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 550 conjugate Rabbit Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10111
Rabbit anti-Human IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 594 conjugate Rabbit Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10112
Rabbit anti-Human IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 650 conjugate Rabbit Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10113
Rabbit anti-Human IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 680 conjugate Rabbit Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10114
Rabbit anti-Human IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 755 conjugate Rabbit Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10115
Rabbit anti-Human IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 800 conjugate Rabbit Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10116
Goat anti-Human IgG (H+L), Cross Adsorbed Secondary Antibody, DyLight 350 conjugate Goat Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10117
Goat anti-Human IgG (H+L), Cross Adsorbed Secondary Antibody, DyLight 550 conjugate Goat Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10119
Goat anti-Human IgG (H+L), Cross Adsorbed Secondary Antibody, DyLight 680 conjugate Goat Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10122
Goat anti-Human IgG (H+L), Cross Adsorbed Secondary Antibody, DyLight 800 conjugate Goat Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10124
Donkey anti-Human IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 350 conjugate Donkey Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10125
Donkey anti-Human IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 488 conjugate Donkey Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10126
Donkey anti-Human IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 550 conjugate Donkey Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10127
Donkey anti-Human IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 594 conjugate Donkey Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10128
Donkey anti-Human IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 650 conjugate Donkey Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10129
Donkey anti-Human IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 680 conjugate Donkey Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10130
Donkey anti-Human IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 755 conjugate Donkey Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10131
Donkey anti-Human IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 800 conjugate Donkey Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10132
Goat anti-Human IgG Fc Cross Adsorbed Secondary Antibody, DyLight 350 conjugate Goat Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10133
Goat anti-Human IgG Fc Cross Adsorbed Secondary Antibody, DyLight 488 conjugate Goat Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10134
Goat anti-Human IgG Fc Cross Adsorbed Secondary Antibody, DyLight 550 conjugate Goat Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10135
Goat anti-Human IgG Fc Cross Adsorbed Secondary Antibody, DyLight 594 conjugate Goat Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10136
Goat anti-Human IgG Fc Cross Adsorbed Secondary Antibody, DyLight 650 conjugate Goat Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10137
Goat anti-Human IgG Fc Cross Adsorbed Secondary Antibody, DyLight 680 conjugate Goat Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10138
Goat anti-Human IgG Fc Cross Adsorbed Secondary Antibody, DyLight 755 conjugate Goat Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10139
Goat anti-Human IgG Fc Cross Adsorbed Secondary Antibody, DyLight 800 conjugate Goat Hu WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10140
Goat anti-Mouse IgM Cross Adsorbed Secondary Antibody, DyLight 350 conjugate Goat Ms WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10149
Goat anti-Mouse IgM Cross Adsorbed Secondary Antibody, DyLight 488 conjugate Goat Ms WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10150
Goat anti-Mouse IgM Cross Adsorbed Secondary Antibody, DyLight 550 conjugate Goat Ms WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10151
Goat anti-Mouse IgM Cross Adsorbed Secondary Antibody, DyLight 594 conjugate Goat Ms WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10152
Goat anti-Mouse IgM Cross Adsorbed Secondary Antibody, DyLight 650 conjugate Goat Ms WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10153
Goat anti-Mouse IgM Cross Adsorbed Secondary Antibody, DyLight 680 conjugate Goat Ms WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10154
Goat anti-Mouse IgM Cross Adsorbed Secondary Antibody, DyLight 755 conjugate Goat Ms WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10155
Goat anti-Mouse IgM Cross Adsorbed Secondary Antibody, DyLight 800 conjugate Goat Ms WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10156
Rabbit anti-Mouse IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 350 conjugate Rabbit Ms WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10157
Rabbit anti-Mouse IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 488 conjugate Rabbit Ms WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10158
Rabbit anti-Mouse IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 550 conjugate Rabbit Ms WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10159
Rabbit anti-Mouse IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 594 conjugate Rabbit Ms WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10160
Rabbit anti-Mouse IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 650 conjugate Rabbit Ms WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10161
Rabbit anti-Mouse IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 680 conjugate Rabbit Ms WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10162
Rabbit anti-Mouse IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 755 conjugate Rabbit Ms WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10163
Rabbit anti-Mouse IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 800 conjugate Rabbit Ms WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10164
Donkey anti-Mouse IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 350 conjugate Donkey Ms WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10165
Donkey anti-Mouse IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 488 conjugate Donkey Ms WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10166
Donkey anti-Mouse IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 550 conjugate Donkey Ms WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10167
Donkey anti-Mouse IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 594 conjugate Donkey Ms WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10168
Donkey anti-Mouse IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 650 conjugate Donkey Ms WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10169
Donkey anti-Mouse IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 680 conjugate Donkey Ms WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10170
Donkey anti-Mouse IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 755 conjugate Donkey Ms WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10171
Donkey anti-Mouse IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 800 conjugate Donkey Ms WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10172
Goat anti-Mouse IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 550 conjugate Goat Ms WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10173
Goat anti-Mouse IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 650 conjugate Goat Ms WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10174
Goat anti-Mouse IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 755 conjugate Goat Ms WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10175
Goat anti-Mouse IgG (H+L) Cross Adsorbed Secondary Antibody, DyLight 800 conjugate Goat Ms WB, IF, ICC, IHC, FACS, IP 00 500 ug SA5-10176
Goat anti-Mouse IgG (H&L) Secondary Antibody, DyLight 800 4X PEG conjugate Goat Ms WB, IF, IHC, IP 00 1 ml SA5-35521
Goat anti-Rabbit IgG (H&L) Secondary Antibody, DyLight 800 4X PEG conjugate Goat Rb WB, IF, IHC, IP 10 1 ml SA5-35571
Streptavidin Protein, DyLight 405 conjugate WB, IF, ICC, IHC, IP, ELISA 00 1 ml 21831
Streptavidin Protein, DyLight 488 conjugate WB, IF, ICC, IHC, IP, ELISA 00 1 ml 21832
Streptavidin Protein, DyLight 594 conjugate WB, IF, ICC, IHC, IP, ELISA 00 1 ml 21842
Streptavidin Protein, DyLight 633 conjugate WB, IF, ICC, IHC, IP, ELISA 00 1 ml 21844
Streptavidin Protein, DyLight 680 conjugate WB, IF, ICC, IHC, IP, ELISA 00 1 ml 21848
Streptavidin Protein, DyLight 800 conjugate WB, IF, ICC, IHC, IP, ELISA 00 1 ml 21851
NeutrAvidin Protein, DyLight 405 conjugate WB, IF, ICC, IHC, IP 00 1 ml 22831
NeutrAvidin Protein, DyLight 488 conjugate WB, IF, ICC, IHC, IP, ELISA 00 1 ml 22832
NeutrAvidin Protein, DyLight 594 conjugate WB, IF, ICC, IHC, IP, ELISA 00 1 ml 22842
NeutrAvidin Protein, DyLight 633 conjugate WB, IF, ICC, IHC, IP, ELISA 00 1 ml 22844
NeutrAvidin Protein, DyLight 680 conjugate WB, IF, ICC, IHC, IP, ELISA 00 1 ml 22848
Neutravidin Protein, DyLight 800 conjugate WB, IF, ICC, IHC, IP, ELISA 00 1 ml 22853
Phalloidin Control , DyLight 350 conjugate Many IF, ICC, IHC 50 300 units 21830
Phalloidin Control , DyLight 488 conjugate Many IF, ICC, IHC 50 300 units 21833
Phalloidin Control , DyLight 554 conjugate Many IF, ICC, IHC 20 300 units 21834
Phalloidin Control , DyLight 550 conjugate Many IF, ICC, IHC 80 300 units 21835
Phalloidin Control , DyLight 594 conjugate Many IF, ICC, IHC 20 300 units 21836
Phalloidin Control , DyLight 650 conjugate Many IF, ICC, IHC 70 300 units 21838
Phalloidin Control , DyLight 680 conjugate Many IF, ICC, IHC 40 300 units 21839
Phalloidin Control , DyLight 633 conjugate Many IF, ICC, IHC 50 300 units 21840
Product Specific Information
DyLight Fluorophore Highlights:

1. Bright fluorescence – intense emission provides superior sensitivity and requires less conjugate. 2. Narrow emission spectra – negligible bleed-through between fluorophore channels enables multi-color detection. 3. Excellent photostability – exceptional resistance to photobleaching enables fluorescence imaging under the most demanding conditions (e.g., structured illumination, 4Pi microscopy). 4. Buffer stability – conjugated fluorophores are completely stable at pH 4-9. 5. Instrument compatibility – excitation and emission spectra correspond with filter sets and laser settings of all popular fluorescence instrumentation.
Product Images
Filter :
  • IF using 21830
    Immunofluorescence with anti-Phalloidin  Control, DyLight 350 conjugate (21830)

    Immunofluorescence with anti-Phalloidin Control, DyLight 350 conjugate (21830)

    Immunofluorescent analysis of Phalloidin (blue) and alpha-Tubulin (green) in NIH 3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with an alpha-Tubulin monoclonal antibody (Product # MA1-19162) at a dilution of 1:1000 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 350 Phalloidin (Product # 21830) at a dilution of 1:120 (2.5units/ml final concentration) and nuclei (red) were stained with DRAQ5 (Product # 62254) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
     
  • IF using 21830
    Immunofluorescence with anti-Phalloidin  Control, DyLight 350 conjugate (21830)

    Immunofluorescence with anti-Phalloidin Control, DyLight 350 conjugate (21830)

    Immunofluorescent analysis of Phalloidin (blue) and Calreticulin (green) in A431 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS + 0.1% Triton X-100 (Product # 37525) for 30 minutes at room temperature. Cells were probed with a Calreticulin polyclonal antibody (Product # PA3-900) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with Dylight 488 goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with Dylight 350 Phalloidin (Product # 21830) at a dilution of 1:120 (2.5units/ml final concentration) and nuclei (red) were stained with DRAQ5 (Product # 62254) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
     
  • IF using 21830
    Immunofluorescence with anti-Phalloidin  Control, DyLight 350 conjugate (21830)

    Immunofluorescence with anti-Phalloidin Control, DyLight 350 conjugate (21830)

    Immunofluorescent analysis of Phalloidin (blue) and EGFR (green) in A431 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS + 0.1% Triton X-100 (Product # 37525) for 30 minutes at room temperature. Cells were probed with an EGFR monoclonal antibody (Product # MA5-12880) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with Dylight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with Dylight 350 Phalloidin (Product # 21830) at a dilution of 1:120 (2.5units/ml final concentration) and nuclei (red) were stained with DRAQ5 (Product # 62254) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
     
  • IF using 21830
    Immunofluorescence with anti-Phalloidin  Control, DyLight 350 conjugate (21830)

    Immunofluorescence with anti-Phalloidin Control, DyLight 350 conjugate (21830)

    Immunofluorescent analysis of Phalloidin (blue) and PDI (green) in NIH 3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS + 0.1% Triton X-100 (Product # 37525) for 30 minutes at room temperature. Cells were probed with a PDI monoclonal antibody (Product # MA3-019) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with Dylight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with Dylight 350 Phalloidin (Product # 21830) at a dilution of 1:120 (2.5units/ml final concentration) and nuclei (red) were stained with DRAQ5 (Product # 62254) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
     
  • IF using 21830
    Immunofluorescence with anti-Phalloidin  Control, DyLight 350 conjugate (21830)

    Immunofluorescence with anti-Phalloidin Control, DyLight 350 conjugate (21830)

    Immunofluorescent analysis of Phalloidin in U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 2% BSA in PBS (Product # 37525) containing 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with DyLight 350 Phalloidin (Product # 21830) or Alexa Fluor® 350 Phalloidin, each diluted to a final concentration of 5 units/ml in PBS, for 30 minutes. Nuclei were stained with DRAQ5 dye (Product # 62254). Images were taken on a Zeiss Axio Observer Z1 microscope with a 20X objective.
     
  • IF using 21833
    Immunofluorescence with anti-Phalloidin  Control, DyLight 488 conjugate (21833)

    Immunofluorescence with anti-Phalloidin Control, DyLight 488 conjugate (21833)

    Immunofluorescent analysis of Phalloidin (green) in primary cultured cortical neurons. Primary cortical neurons were isolated and cultured using the Primary Neuron Isolation Kit (Product # 88280). Neurons were grown in a 35mm glass bottom culture dish at a density of 500,000 cells per well. At day 21, neurons were fixed with 4% paraformaldehyde and probed with Dylight 488 Phalloidin (Product # 21833) at a dilution of 1:500 in HBSS (Product # 88284) for 10-15 minutes at room temperature. Cells were washed twice with HBSS, and visualized by fluorescence microscopy. Images were taken at 40X magnification on a Carl Zeiss microscope (AxioVision Rel. 4.7). Note: Representative Phalloidin-labeled dendritic segments studded with mature dendritic spines from primary cultured cortical neurons are shown.
     
  • IF using 21833
    Immunofluorescence with anti-Phalloidin  Control, DyLight 488 conjugate (21833)

    Immunofluorescence with anti-Phalloidin Control, DyLight 488 conjugate (21833)

    Immunofluorescent analysis of Phalloidin (green) and Calreticulin (red) in U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a Calreticulin polyclonal antibody (Product # PA3-900) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 633 goat anti-rabbit IgG secondary antibody (Product # 35562) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 488 Phalloidin (Product # 21833) at a dilution of 1:300 (1 unit/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Thermo Scientific ArrayScan VTI at 20X magnification.
     
  • IF using 21833
    Immunofluorescence with anti-Phalloidin  Control, DyLight 488 conjugate (21833)

    Immunofluorescence with anti-Phalloidin Control, DyLight 488 conjugate (21833)

    Immunofluorescent analysis of Phalloidin in U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 2% BSA in PBS (Product # 37525) containing 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with DyLight 488 Phalloidin (Product # 21833) or Alexa Fluor® 488 Phalloidin, each diluted to a final concentration of 1 unit/ml in PBS, for 30 minutes. Nuclei were stained with Hoeschst dye (Product # 62249). Images were taken on a Zeiss Axio Observer Z1 microscope with a 20X objective.
     
  • IF using 21833
    Immunofluorescence with anti-Phalloidin  Control, DyLight 488 conjugate (21833)

    Immunofluorescence with anti-Phalloidin Control, DyLight 488 conjugate (21833)

    Immunofluorescent analysis of Phalloidin (green) and PDI (red) in U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a PDI monoclonal antibody (Product # MA3-019) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 550 goat anti-mouse IgG secondary antibody (Product # 84540) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 488 Phalloidin (Product # 21833) at a dilution of 1:300 (1 unit/ml final concentration) for 30 minutes. Images were taken on a Thermo Scientific ArrayScan VTI at 20X magnification.
     
  • IF using 21833
    Immunofluorescence with anti-Phalloidin  Control, DyLight 488 conjugate (21833)

    Immunofluorescence with anti-Phalloidin Control, DyLight 488 conjugate (21833)

    Immunofluorescent analysis of Phalloidin (green) and TGN38 (purple) in U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a TGN38 monoclonal antibody (Product # MA3-063) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 680 goat anti-mouse IgG secondary antibody (Product # 35518) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 488 Phalloidin (Product # 21833) at a dilution of 1:300 (1 unit/ml final concentration) for 30 minutes. Images were taken on a Thermo Scientific ArrayScan VTI at 20X magnification.
     
  • IF using 21834
    Immunofluorescence with anti-Phalloidin  Control, DyLight 554 conjugate (21834)

    Immunofluorescence with anti-Phalloidin Control, DyLight 554 conjugate (21834)

    Immunofluorescent analysis of Calreticulin (green) U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a Calreticulin polyclonal antibody (Product # PA3-900) at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:250 for 30 minutes at room temperature. Actin filaments (red) were stained with DyLight 554 Phalloidin (Product # 21834) at a dilution of 1:300 (1unit/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1ug/mL. Images were taken on a Thermo Scientific ArrayScan VTI at 20X magnification.
     
  • IF using 21834
    Immunofluorescence with anti-Phalloidin  Control, DyLight 554 conjugate (21834)

    Immunofluorescence with anti-Phalloidin Control, DyLight 554 conjugate (21834)

    Immunofluorescent analysis of Phalloidin in U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 2% BSA in PBS (Product # 37525) containing 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with DyLight 554 Phalloidin (Product # 21834) or Alexa Fluor® 555 Phalloidin, each diluted to a final concentration of 1 unit/ml in PBS, for 30 minutes. Nuclei were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Zeiss Axio Observer Z1 microscope with a 20X objective.
     
  • IF using 21835
    Immunofluorescence with anti-Phalloidin  Control, DyLight 550 conjugate (21835)

    Immunofluorescence with anti-Phalloidin Control, DyLight 550 conjugate (21835)

    Immunofluorescent analysis of Phalloidin (orange) and alpha-Tubulin (green) in NIH 3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with an alpha-Tubulin monoclonal antibody (Product # MA1-19162) at a dilution of 1:1000 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 550 Phalloidin (Product # 21835) at a dilution of 1:120 (2.5 units/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
     
  • IF using 21835
    Immunofluorescence with anti-Phalloidin  Control, DyLight 550 conjugate (21835)

    Immunofluorescence with anti-Phalloidin Control, DyLight 550 conjugate (21835)

    Immunofluorescent analysis of Phalloidin (orange) and Calreticulin (green) in A431 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a Calreticulin polyclonal antibody (Product # PA3-900) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 550 Phalloidin (Product # 21835) at a dilution of 1:120 (2.5 units/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
     
  • IF using 21835
    Immunofluorescence with anti-Phalloidin  Control, DyLight 550 conjugate (21835)

    Immunofluorescence with anti-Phalloidin Control, DyLight 550 conjugate (21835)

    Immunofluorescent analysis of Phalloidin (orange) and Cytochrome c (green) in NIH 3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a Cytochrome c monoclonal antibody (Product # MA5-11823) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 550 Phalloidin (Product # 21835) at a dilution of 1:120 (2.5 units/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
     
  • IF using 21835
    Immunofluorescence with anti-Phalloidin  Control, DyLight 550 conjugate (21835)

    Immunofluorescence with anti-Phalloidin Control, DyLight 550 conjugate (21835)

    Immunofluorescent analysis of Phalloidin (orange) and EGFR (green) in A431 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with an EGFR monoclonal antibody (Product # MA5-12880) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 550 Phalloidin (Product # 21835) at a dilution of 1:120 (2.5 units/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
     
  • IF using 21835
    Immunofluorescence with anti-Phalloidin  Control, DyLight 550 conjugate (21835)

    Immunofluorescence with anti-Phalloidin Control, DyLight 550 conjugate (21835)

    Immunofluorescent analysis of Phalloidin (orange) and Ezrin (green) in A431 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with an Ezrin monoclonal antibody (Product # MA5-13862) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 550 Phalloidin (Product # 21835) at a dilution of 1:120 (2.5 units/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
     
  • IF using 21835
    Immunofluorescence with anti-Phalloidin  Control, DyLight 550 conjugate (21835)

    Immunofluorescence with anti-Phalloidin Control, DyLight 550 conjugate (21835)

    Immunofluorescent analysis of Phalloidin (orange) and HDAC2 (green) in A431 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with an HDAC2 polyclonal antibody (Product # PA1-861) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 550 Phalloidin (Product # 21835) at a dilution of 1:120 (2.5 units/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
     
  • IF using 21835
    Immunofluorescence with anti-Phalloidin  Control, DyLight 550 conjugate (21835)

    Immunofluorescence with anti-Phalloidin Control, DyLight 550 conjugate (21835)

    Immunofluorescent analysis of Phalloidin (orange) and PDI (green) in NIH 3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a PDI monoclonal antibody (Product # MA3-019) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 550 Phalloidin (Product # 21835) at a dilution of 1:120 (2.5 units/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
     
  • IF using 21835
    Immunofluorescence with anti-Phalloidin  Control, DyLight 550 conjugate (21835)

    Immunofluorescence with anti-Phalloidin Control, DyLight 550 conjugate (21835)

    Immunofluorescent analysis of Phalloidin in U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 2% BSA in PBS (Product # 37525) containing 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with DyLight 550 Phalloidin (Product # 21835) or Alexa Fluor® 555 Phalloidin, each diluted to a final concentration of 1 unit/ml in PBS, for 30 minutes. Nuclei were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Zeiss Axio Observer Z1 microscope with a 20X objective.
     
  • IF using 21836
    Immunofluorescence with anti-Phalloidin  Control, DyLight 594 conjugate (21836)

    Immunofluorescence with anti-Phalloidin Control, DyLight 594 conjugate (21836)

    Immunofluorescent analysis of Phalloidin (red) and NFkB (green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a NFkB polyclonal antibody for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 594 Phalloidin (Product # 21836) at a dilution of 1:300 (1 unit/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62254) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
     
  • IF using 21836
    Immunofluorescence with anti-Phalloidin  Control, DyLight 594 conjugate (21836)

    Immunofluorescence with anti-Phalloidin Control, DyLight 594 conjugate (21836)

    Immunofluorescent analysis of Phalloidin in U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 2% BSA in PBS (Product # 37525) containing 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with DyLight 594 Phalloidin (Product # 21836) or Alexa Fluor® 594 Phalloidin, each diluted to a final concentration of 1 unit/ml in PBS, for 30 minutes. Nuclei were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Zeiss Axio Observer Z1 microscope with a 20X objective.
     
  • IF using 21838
    Immunofluorescence with anti-Phalloidin  Control, DyLight 650 conjugate (21838)

    Immunofluorescence with anti-Phalloidin Control, DyLight 650 conjugate (21838)

    Immunofluorescent analysis of Phalloidin (purple) and alpha-Tubulin (green) in NIH 3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with an alpha-Tubulin monoclonal antibody (Product # MA1-19162) at a dilution of 1:1000 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 650 Phalloidin (Product # 21838) at a dilution of 1:120 (2.5 units/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
     
  • IF using 21838
    Immunofluorescence with anti-Phalloidin  Control, DyLight 650 conjugate (21838)

    Immunofluorescence with anti-Phalloidin Control, DyLight 650 conjugate (21838)

    Immunofluorescent analysis of Phalloidin (purple) and Calreticulin (green) in A431 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a Calreticulin polyclonal antibody (Product # PA3-900) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 650 Phalloidin (Product # 21838) at a dilution of 1:120 (2.5 units/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
     
  • IF using 21838
    Immunofluorescence with anti-Phalloidin  Control, DyLight 650 conjugate (21838)

    Immunofluorescence with anti-Phalloidin Control, DyLight 650 conjugate (21838)

    Immunofluorescent analysis of Phalloidin (purple) and Cytochrome c (green) in NIH 3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a Cytochrome c monoclonal antibody (Product # MA5-11823) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 650 Phalloidin (Product # 21838) at a dilution of 1:120 (2.5 units/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
     
  • IF using 21838
    Immunofluorescence with anti-Phalloidin  Control, DyLight 650 conjugate (21838)

    Immunofluorescence with anti-Phalloidin Control, DyLight 650 conjugate (21838)

    Immunofluorescent analysis of Phalloidin (purple) and EGFR (green) in A431 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with an EGFR monoclonal antibody (Product # MA5-12880) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 650 Phalloidin (Product # 21838) at a dilution of 1:120 (2.5 units/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
     
  • IF using 21838
    Immunofluorescence with anti-Phalloidin  Control, DyLight 650 conjugate (21838)

    Immunofluorescence with anti-Phalloidin Control, DyLight 650 conjugate (21838)

    Immunofluorescent analysis of Phalloidin (purple) and HDAC2 (green) in A431 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a HDAC2 polyclonal antibody (Product # PA1-861) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 650 Phalloidin (Product # 21838) at a dilution of 1:120 (2.5 units/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
     
  • IF using 21838
    Immunofluorescence with anti-Phalloidin  Control, DyLight 650 conjugate (21838)

    Immunofluorescence with anti-Phalloidin Control, DyLight 650 conjugate (21838)

    Immunofluorescent analysis of Phalloidin (purple) and PDI (green) in NIH 3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a PDI monoclonal antibody (Product # MA3-019) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 650 Phalloidin (Product # 21838) at a dilution of 1:120 (2.5 units/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
     
  • IF using 21838
    Immunofluorescence with anti-Phalloidin  Control, DyLight 650 conjugate (21838)

    Immunofluorescence with anti-Phalloidin Control, DyLight 650 conjugate (21838)

    Immunofluorescent analysis of Phalloidin in U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 2% BSA in PBS (Product # 37525) containing 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with DyLight 650 Phalloidin (Product # 21838) or Alexa Fluor® 647 Phalloidin, each diluted to a final concentration of 2.5 units/ml in PBS, for 30 minutes. Nuclei were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Zeiss Axio Observer Z1 microscope with a 20X objective.
     
  • IF using 21839
    Immunofluorescence with anti-Phalloidin  Control, DyLight 680 conjugate (21839)

    Immunofluorescence with anti-Phalloidin Control, DyLight 680 conjugate (21839)

    Immunofluorescent analysis of Phalloidin (dark red) and alpha-Tubulin (green) in NIH 3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with an alpha-Tubulin monoclonal antibody (Product # MA1-19162) at a dilution of 1:1000 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 680 Phalloidin (Product # 21839) at a dilution of 1:120 (2.5 units/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
     
  • IF using 21839
    Immunofluorescence with anti-Phalloidin  Control, DyLight 680 conjugate (21839)

    Immunofluorescence with anti-Phalloidin Control, DyLight 680 conjugate (21839)

    Immunofluorescent analysis of Phalloidin (dark red) and Cytochrome c (green) in NIH 3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a Cytochrome c monoclonal antibody (Product # MA5-11823) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 680 Phalloidin (Product # 21839) at a dilution of 1:120 (2.5 units/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
     
  • IF using 21839
    Immunofluorescence with anti-Phalloidin  Control, DyLight 680 conjugate (21839)

    Immunofluorescence with anti-Phalloidin Control, DyLight 680 conjugate (21839)

    Immunofluorescent analysis of Phalloidin (dark red) and Ezrin (green) in A431 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with an Ezrin monoclonal antibody (Product # MA5-13862) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 680 Phalloidin (Product # 21839) at a dilution of 1:120 (2.5 units/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
     
  • IF using 21839
    Immunofluorescence with anti-Phalloidin  Control, DyLight 680 conjugate (21839)

    Immunofluorescence with anti-Phalloidin Control, DyLight 680 conjugate (21839)

    Immunofluorescent analysis of Phalloidin in U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 2% BSA in PBS (Product # 37525) containing 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with DyLight 680 Phalloidin (Product # 21839) or Alexa Fluor® 680 Phalloidin, each diluted to a final concentration of 2.5 units/ml in PBS, for 30 minutes. Nuclei were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Zeiss Axio Observer Z1 microscope with a 20X objective.
     
  • IF using 21840
    Immunofluorescence with anti-Phalloidin  Control, DyLight 633 conjugate (21840)

    Immunofluorescence with anti-Phalloidin Control, DyLight 633 conjugate (21840)

    Immunofluorescent analysis of Phalloidin (magenta) and alpha-Tubulin (green) in NIH 3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with an alpha-Tubulin monoclonal antibody (Product # MA1-19162) at a dilution of 1:1000 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 633 Phalloidin (Product # 21840) at a dilution of 1:120 (2.5units/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
     
  • IF using 21840
    Immunofluorescence with anti-Phalloidin  Control, DyLight 633 conjugate (21840)

    Immunofluorescence with anti-Phalloidin Control, DyLight 633 conjugate (21840)

    Immunofluorescent analysis of Phalloidin (magenta) and Cytochrome c (green) in NIH 3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a Cytochrome c monoclonal antibody (Product # MA5-11823) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 633 Phalloidin (Product # 21840) at a dilution of 1:120 (2.5units/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
     
  • IF using 21840
    Immunofluorescence with anti-Phalloidin  Control, DyLight 633 conjugate (21840)

    Immunofluorescence with anti-Phalloidin Control, DyLight 633 conjugate (21840)

    Immunofluorescent analysis of Phalloidin (magenta) and Ezrin (green) in A431 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with an Ezrin monoclonal antibody (Product # MA5-13862) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 633 Phalloidin (Product # 21840) at a dilution of 1:120 (2.5units/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
     
  • IF using 21840
    Immunofluorescence with anti-Phalloidin  Control, DyLight 633 conjugate (21840)

    Immunofluorescence with anti-Phalloidin Control, DyLight 633 conjugate (21840)

    Immunofluorescent analysis of Phalloidin (magenta) and HDAC2 (green) in A431 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with an HDAC2 polyclonal antibody (Product # PA1-861) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 633 Phalloidin (Product # 21840) at a dilution of 1:120 (2.5units/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
     
  • IF using 21840
    Immunofluorescence with anti-Phalloidin  Control, DyLight 633 conjugate (21840)

    Immunofluorescence with anti-Phalloidin Control, DyLight 633 conjugate (21840)

    Immunofluorescent analysis of Phalloidin in U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 2% BSA in PBS (Product # 37525) containing 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with DyLight 633 Phalloidin (Product # 21840) or Alexa Fluor® 633 Phalloidin, each diluted to a final concentration of 2.5 units/ml in PBS, for 30 minutes. Nuclei were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Zeiss Axio Observer Z1 microscope with a 20X objective.
     
  • WB using SA5-35571
    Western Blot with anti-Rabbit IgG (H&L) Polyclonal Secondary Antibody, DyLight 800 4X PEG conjugate (SA5-35571)

    Western Blot with anti-Rabbit IgG (H&L) Polyclonal Secondary Antibody, DyLight 800 4X PEG conjugate (SA5-35571)

    Western blot analysis of Heat Shock Protein 90 (Hsp90) was performed by loading 2-fold serial dilutions of HeLa whole cell lysate (starting at 10ug) and 10ul of the PageRuler Prestained NIR Protein Ladder (Product # 26635) per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to Low Fluorescence PVDF Membranes (Product # 22860) and blocked with SEA BLOCK Blocking Buffer (Product # 37527) for 1 hour. Membranes were probed with an Hsp90 polyclonal antibody (Product # PA3-013) at a dilution of 1:1000 overnight at 4°C on a rocking platform, washed with TBST, and probed with either a DyLight 800-4xPEG-conjugated goat anti-rabbit IgG (Product # SA5-35571), a DyLight 800-conjugated goat anti-rabbit IgG (Product # 35571), or a CF*790-conjugated goat anti-rabbit IgG (Biotium) secondary antibody all at dilutions of 1:25,000 for 45 minutes. Blots were imaged on an infrared imaging system.
     
 
 

PubMed References
Immunocytochemistry
  Species / Dilution Summary
  Rt / Not Cited
35552 was used in immunocytochemistry to investigate the regulation of survivin stability by EGF

BMC Mol Biol. 2010 Aug 31;11():66.
"EGF regulates survivin stability through the Raf-1/ERK pathway in insulin-secreting pancreatic ?-cells."
Author(s): Wang H, Gambosova K, Cooper ZA, Holloway MP, Kassai A, Izquierdo D, Cleveland K, Boney CM, Altura RA
Number of Citations: 1
(See PubMed article )
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