DyLight Conjugated Phalloidins

The Thermo Scientific DyLight Phalloidin Conjugates bind to and fluorescently label polymerized filamentous actin (F-actin), which is commonly found in the cellular cytoskeleton networks. The phalloidin conjugates have absorption spectra ranging from 350-690nm and are useful for histology and cell biology applications requiring visualization of the F-actin cytoskeleton. In imaging applications, the conjugates are often used as a cytoplasmic counterstain (similar to DAPI or Hoechst for the nucleus) in fixed cells, permeabilized cells and cell-free experiments.

The DyLight Dyes conjugated to phalloidin are excellent alternatives to Alexa Fluor Dyes (Life Tech). The DyLight Dyes have fluorescence characteristics (e.g., brightness, photostability, pH insensitivity and water solubility) equivalent, or superior to similar Alexa Fluor Dyes. In addition, the high-fluorescence intensity of DyLight Dyes provides outstanding sensitivity and requires using less conjugate in most applications.

Related Links:
DAPI Nuclear Counterstain
Hoechst 33342 Fluorescent Stain
DRAQ5 Fluoescent Probe
Whole Cell Fluorescent Stains

Select from the filters below to narrow down the list of Phalloidin conjugates to meet your specifications. Phalloidin conjguates are validated in all applications listed. Where available, product specific references are provided in the individual datasheets along with figure legends and images showing the Phalloidin conjugate testing data by application.


Filter Results
By Type
Control (8)
By Class
By Target Species
[Many] Many (8)
By Host
By Application
IHC (8)
ICC (8)
IF (8)
By Clone
By Label
Labeled (8)
DyLight 650 (1)
DyLight 680 (1)
By PTM
Not PTM-specific (8)
Product Description Host Target Species Applications Imgs / Refs Size Product # Price
Phalloidin Control , DyLight 550 conjugate Many IF, ICC, IHC 80 300 units 21835 $360.00
Phalloidin Control , DyLight 650 conjugate Many IF, ICC, IHC 70 300 units 21838 $360.00
Phalloidin Control , DyLight 350 conjugate Many IF, ICC, IHC 50 300 units 21830 $360.00
Phalloidin Control , DyLight 633 conjugate Many IF, ICC, IHC 50 300 units 21840 $360.00
Phalloidin Control , DyLight 488 conjugate Many IF, ICC, IHC 40 300 units 21833 $360.00
Phalloidin Control , DyLight 680 conjugate Many IF, ICC, IHC 40 300 units 21839 $360.00
Phalloidin Control , DyLight 554 conjugate Many IF, ICC, IHC 20 300 units 21834 $360.00
Phalloidin Control , DyLight 594 conjugate Many IF, ICC, IHC 20 300 units 21836 $360.00
Product Specific Information

Phalloidin is a bicyclic peptide that belongs to a family of toxins isolated from the deadly Amanita phalloides “death cap” mushroom. It specifically binds in a stoichiometric ratio of approximately one phallotoxin per actin subunit in both muscle and non-muscle cells to polymerized F-actin with similar affinity for both large and small filaments. The dynamics of the actin polymerization in cells is important for a variety of cellular processes, including cell motility, cell shape, muscular contraction and cytokinesis.

Product Images
Filter :
  • IF using 21830
    Immunofluorescence with anti-Phalloidin  Control, DyLight 350 conjugate (21830)

    Immunofluorescence with anti-Phalloidin Control, DyLight 350 conjugate (21830)

    Immunofluorescent analysis of Phalloidin (blue) and alpha-Tubulin (green) in NIH 3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with an alpha-Tubulin monoclonal antibody (Product # MA1-19162) at a dilution of 1:1000 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 350 Phalloidin (Product # 21830) at a dilution of 1:120 (2.5units/ml final concentration) and nuclei (red) were stained with DRAQ5 (Product # 62254) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
     
  • IF using 21830
    Immunofluorescence with anti-Phalloidin  Control, DyLight 350 conjugate (21830)

    Immunofluorescence with anti-Phalloidin Control, DyLight 350 conjugate (21830)

    Immunofluorescent analysis of Phalloidin (blue) and Calreticulin (green) in A431 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS + 0.1% Triton X-100 (Product # 37525) for 30 minutes at room temperature. Cells were probed with a Calreticulin polyclonal antibody (Product # PA3-900) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with Dylight 488 goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with Dylight 350 Phalloidin (Product # 21830) at a dilution of 1:120 (2.5units/ml final concentration) and nuclei (red) were stained with DRAQ5 (Product # 62254) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
     
  • IF using 21830
    Immunofluorescence with anti-Phalloidin  Control, DyLight 350 conjugate (21830)

    Immunofluorescence with anti-Phalloidin Control, DyLight 350 conjugate (21830)

    Immunofluorescent analysis of Phalloidin (blue) and EGFR (green) in A431 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS + 0.1% Triton X-100 (Product # 37525) for 30 minutes at room temperature. Cells were probed with an EGFR monoclonal antibody (Product # MA5-12880) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with Dylight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with Dylight 350 Phalloidin (Product # 21830) at a dilution of 1:120 (2.5units/ml final concentration) and nuclei (red) were stained with DRAQ5 (Product # 62254) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
     
  • IF using 21830
    Immunofluorescence with anti-Phalloidin  Control, DyLight 350 conjugate (21830)

    Immunofluorescence with anti-Phalloidin Control, DyLight 350 conjugate (21830)

    Immunofluorescent analysis of Phalloidin (blue) and PDI (green) in NIH 3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS + 0.1% Triton X-100 (Product # 37525) for 30 minutes at room temperature. Cells were probed with a PDI monoclonal antibody (Product # MA3-019) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with Dylight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with Dylight 350 Phalloidin (Product # 21830) at a dilution of 1:120 (2.5units/ml final concentration) and nuclei (red) were stained with DRAQ5 (Product # 62254) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
     
  • IF using 21830
    Immunofluorescence with anti-Phalloidin  Control, DyLight 350 conjugate (21830)

    Immunofluorescence with anti-Phalloidin Control, DyLight 350 conjugate (21830)

    Immunofluorescent analysis of Phalloidin in U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 2% BSA in PBS (Product # 37525) containing 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with DyLight 350 Phalloidin (Product # 21830) or Alexa Fluor® 350 Phalloidin, each diluted to a final concentration of 5 units/ml in PBS, for 30 minutes. Nuclei were stained with DRAQ5 dye (Product # 62254). Images were taken on a Zeiss Axio Observer Z1 microscope with a 20X objective.
     
  • IF using 21833
    Immunofluorescence with anti-Phalloidin  Control, DyLight 488 conjugate (21833)

    Immunofluorescence with anti-Phalloidin Control, DyLight 488 conjugate (21833)

    Immunofluorescent analysis of Phalloidin (green) and Calreticulin (red) in U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a Calreticulin polyclonal antibody (Product # PA3-900) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 633 goat anti-rabbit IgG secondary antibody (Product # 35562) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 488 Phalloidin (Product # 21833) at a dilution of 1:300 (1 unit/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Thermo Scientific ArrayScan VTI at 20X magnification.
     
  • IF using 21833
    Immunofluorescence with anti-Phalloidin  Control, DyLight 488 conjugate (21833)

    Immunofluorescence with anti-Phalloidin Control, DyLight 488 conjugate (21833)

    Immunofluorescent analysis of Phalloidin in U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 2% BSA in PBS (Product # 37525) containing 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with DyLight 488 Phalloidin (Product # 21833) or Alexa Fluor® 488 Phalloidin, each diluted to a final concentration of 1 unit/ml in PBS, for 30 minutes. Nuclei were stained with Hoeschst dye (Product # 62249). Images were taken on a Zeiss Axio Observer Z1 microscope with a 20X objective.
     
  • IF using 21833
    Immunofluorescence with anti-Phalloidin  Control, DyLight 488 conjugate (21833)

    Immunofluorescence with anti-Phalloidin Control, DyLight 488 conjugate (21833)

    Immunofluorescent analysis of Phalloidin (green) and PDI (red) in U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a PDI monoclonal antibody (Product # MA3-019) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 550 goat anti-mouse IgG secondary antibody (Product # 84540) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 488 Phalloidin (Product # 21833) at a dilution of 1:300 (1 unit/ml final concentration) for 30 minutes. Images were taken on a Thermo Scientific ArrayScan VTI at 20X magnification.
     
  • IF using 21833
    Immunofluorescence with anti-Phalloidin  Control, DyLight 488 conjugate (21833)

    Immunofluorescence with anti-Phalloidin Control, DyLight 488 conjugate (21833)

    Immunofluorescent analysis of Phalloidin (green) and TGN38 (purple) in U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a TGN38 monoclonal antibody (Product # MA3-063) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 680 goat anti-mouse IgG secondary antibody (Product # 35518) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 488 Phalloidin (Product # 21833) at a dilution of 1:300 (1 unit/ml final concentration) for 30 minutes. Images were taken on a Thermo Scientific ArrayScan VTI at 20X magnification.
     
  • IF using 21834
    Immunofluorescence with anti-Phalloidin  Control, DyLight 554 conjugate (21834)

    Immunofluorescence with anti-Phalloidin Control, DyLight 554 conjugate (21834)

    Immunofluorescent analysis of Calreticulin (green) U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a Calreticulin polyclonal antibody (Product # PA3-900) at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:250 for 30 minutes at room temperature. Actin filaments (red) were stained with DyLight 554 Phalloidin (Product # 21834) at a dilution of 1:300 (1unit/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1ug/mL. Images were taken on a Thermo Scientific ArrayScan VTI at 20X magnification.
     
  • IF using 21834
    Immunofluorescence with anti-Phalloidin  Control, DyLight 554 conjugate (21834)

    Immunofluorescence with anti-Phalloidin Control, DyLight 554 conjugate (21834)

    Immunofluorescent analysis of Phalloidin in U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 2% BSA in PBS (Product # 37525) containing 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with DyLight 554 Phalloidin (Product # 21834) or Alexa Fluor® 555 Phalloidin, each diluted to a final concentration of 1 unit/ml in PBS, for 30 minutes. Nuclei were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Zeiss Axio Observer Z1 microscope with a 20X objective.
     
  • IF using 21835
    Immunofluorescence with anti-Phalloidin  Control, DyLight 550 conjugate (21835)

    Immunofluorescence with anti-Phalloidin Control, DyLight 550 conjugate (21835)

    Immunofluorescent analysis of Phalloidin (orange) and alpha-Tubulin (green) in NIH 3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with an alpha-Tubulin monoclonal antibody (Product # MA1-19162) at a dilution of 1:1000 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 550 Phalloidin (Product # 21835) at a dilution of 1:120 (2.5 units/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
     
  • IF using 21835
    Immunofluorescence with anti-Phalloidin  Control, DyLight 550 conjugate (21835)

    Immunofluorescence with anti-Phalloidin Control, DyLight 550 conjugate (21835)

    Immunofluorescent analysis of Phalloidin (orange) and Calreticulin (green) in A431 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a Calreticulin polyclonal antibody (Product # PA3-900) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 550 Phalloidin (Product # 21835) at a dilution of 1:120 (2.5 units/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
     
  • IF using 21835
    Immunofluorescence with anti-Phalloidin  Control, DyLight 550 conjugate (21835)

    Immunofluorescence with anti-Phalloidin Control, DyLight 550 conjugate (21835)

    Immunofluorescent analysis of Phalloidin (orange) and Cytochrome c (green) in NIH 3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a Cytochrome c monoclonal antibody (Product # MA5-11823) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 550 Phalloidin (Product # 21835) at a dilution of 1:120 (2.5 units/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
     
  • IF using 21835
    Immunofluorescence with anti-Phalloidin  Control, DyLight 550 conjugate (21835)

    Immunofluorescence with anti-Phalloidin Control, DyLight 550 conjugate (21835)

    Immunofluorescent analysis of Phalloidin (orange) and EGFR (green) in A431 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with an EGFR monoclonal antibody (Product # MA5-12880) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 550 Phalloidin (Product # 21835) at a dilution of 1:120 (2.5 units/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
     
  • IF using 21835
    Immunofluorescence with anti-Phalloidin  Control, DyLight 550 conjugate (21835)

    Immunofluorescence with anti-Phalloidin Control, DyLight 550 conjugate (21835)

    Immunofluorescent analysis of Phalloidin (orange) and Ezrin (green) in A431 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with an Ezrin monoclonal antibody (Product # MA5-13862) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 550 Phalloidin (Product # 21835) at a dilution of 1:120 (2.5 units/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
     
  • IF using 21835
    Immunofluorescence with anti-Phalloidin  Control, DyLight 550 conjugate (21835)

    Immunofluorescence with anti-Phalloidin Control, DyLight 550 conjugate (21835)

    Immunofluorescent analysis of Phalloidin (orange) and HDAC2 (green) in A431 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with an HDAC2 polyclonal antibody (Product # PA1-861) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 550 Phalloidin (Product # 21835) at a dilution of 1:120 (2.5 units/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
     
  • IF using 21835
    Immunofluorescence with anti-Phalloidin  Control, DyLight 550 conjugate (21835)

    Immunofluorescence with anti-Phalloidin Control, DyLight 550 conjugate (21835)

    Immunofluorescent analysis of Phalloidin (orange) and PDI (green) in NIH 3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a PDI monoclonal antibody (Product # MA3-019) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 550 Phalloidin (Product # 21835) at a dilution of 1:120 (2.5 units/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
     
  • IF using 21835
    Immunofluorescence with anti-Phalloidin  Control, DyLight 550 conjugate (21835)

    Immunofluorescence with anti-Phalloidin Control, DyLight 550 conjugate (21835)

    Immunofluorescent analysis of Phalloidin in U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 2% BSA in PBS (Product # 37525) containing 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with DyLight 550 Phalloidin (Product # 21835) or Alexa Fluor® 555 Phalloidin, each diluted to a final concentration of 1 unit/ml in PBS, for 30 minutes. Nuclei were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Zeiss Axio Observer Z1 microscope with a 20X objective.
     
  • IF using 21836
    Immunofluorescence with anti-Phalloidin  Control, DyLight 594 conjugate (21836)

    Immunofluorescence with anti-Phalloidin Control, DyLight 594 conjugate (21836)

    Immunofluorescent analysis of Phalloidin (red) and NFkB (green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a NFkB polyclonal antibody for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 594 Phalloidin (Product # 21836) at a dilution of 1:300 (1 unit/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62254) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
     
  • IF using 21836
    Immunofluorescence with anti-Phalloidin  Control, DyLight 594 conjugate (21836)

    Immunofluorescence with anti-Phalloidin Control, DyLight 594 conjugate (21836)

    Immunofluorescent analysis of Phalloidin in U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 2% BSA in PBS (Product # 37525) containing 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with DyLight 594 Phalloidin (Product # 21836) or Alexa Fluor® 594 Phalloidin, each diluted to a final concentration of 1 unit/ml in PBS, for 30 minutes. Nuclei were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Zeiss Axio Observer Z1 microscope with a 20X objective.
     
  • IF using 21838
    Immunofluorescence with anti-Phalloidin  Control, DyLight 650 conjugate (21838)

    Immunofluorescence with anti-Phalloidin Control, DyLight 650 conjugate (21838)

    Immunofluorescent analysis of Phalloidin (purple) and alpha-Tubulin (green) in NIH 3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with an alpha-Tubulin monoclonal antibody (Product # MA1-19162) at a dilution of 1:1000 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 650 Phalloidin (Product # 21838) at a dilution of 1:120 (2.5 units/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
     
  • IF using 21838
    Immunofluorescence with anti-Phalloidin  Control, DyLight 650 conjugate (21838)

    Immunofluorescence with anti-Phalloidin Control, DyLight 650 conjugate (21838)

    Immunofluorescent analysis of Phalloidin (purple) and Calreticulin (green) in A431 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a Calreticulin polyclonal antibody (Product # PA3-900) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 650 Phalloidin (Product # 21838) at a dilution of 1:120 (2.5 units/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
     
  • IF using 21838
    Immunofluorescence with anti-Phalloidin  Control, DyLight 650 conjugate (21838)

    Immunofluorescence with anti-Phalloidin Control, DyLight 650 conjugate (21838)

    Immunofluorescent analysis of Phalloidin (purple) and Cytochrome c (green) in NIH 3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a Cytochrome c monoclonal antibody (Product # MA5-11823) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 650 Phalloidin (Product # 21838) at a dilution of 1:120 (2.5 units/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
     
  • IF using 21838
    Immunofluorescence with anti-Phalloidin  Control, DyLight 650 conjugate (21838)

    Immunofluorescence with anti-Phalloidin Control, DyLight 650 conjugate (21838)

    Immunofluorescent analysis of Phalloidin (purple) and EGFR (green) in A431 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with an EGFR monoclonal antibody (Product # MA5-12880) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 650 Phalloidin (Product # 21838) at a dilution of 1:120 (2.5 units/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
     
  • IF using 21838
    Immunofluorescence with anti-Phalloidin  Control, DyLight 650 conjugate (21838)

    Immunofluorescence with anti-Phalloidin Control, DyLight 650 conjugate (21838)

    Immunofluorescent analysis of Phalloidin (purple) and HDAC2 (green) in A431 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a HDAC2 polyclonal antibody (Product # PA1-861) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 650 Phalloidin (Product # 21838) at a dilution of 1:120 (2.5 units/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
     
  • IF using 21838
    Immunofluorescence with anti-Phalloidin  Control, DyLight 650 conjugate (21838)

    Immunofluorescence with anti-Phalloidin Control, DyLight 650 conjugate (21838)

    Immunofluorescent analysis of Phalloidin (purple) and PDI (green) in NIH 3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a PDI monoclonal antibody (Product # MA3-019) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 650 Phalloidin (Product # 21838) at a dilution of 1:120 (2.5 units/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
     
  • IF using 21838
    Immunofluorescence with anti-Phalloidin  Control, DyLight 650 conjugate (21838)

    Immunofluorescence with anti-Phalloidin Control, DyLight 650 conjugate (21838)

    Immunofluorescent analysis of Phalloidin in U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 2% BSA in PBS (Product # 37525) containing 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with DyLight 650 Phalloidin (Product # 21838) or Alexa Fluor® 647 Phalloidin, each diluted to a final concentration of 2.5 units/ml in PBS, for 30 minutes. Nuclei were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Zeiss Axio Observer Z1 microscope with a 20X objective.
     
  • IF using 21839
    Immunofluorescence with anti-Phalloidin  Control, DyLight 680 conjugate (21839)

    Immunofluorescence with anti-Phalloidin Control, DyLight 680 conjugate (21839)

    Immunofluorescent analysis of Phalloidin (dark red) and alpha-Tubulin (green) in NIH 3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with an alpha-Tubulin monoclonal antibody (Product # MA1-19162) at a dilution of 1:1000 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 680 Phalloidin (Product # 21839) at a dilution of 1:120 (2.5 units/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
     
  • IF using 21839
    Immunofluorescence with anti-Phalloidin  Control, DyLight 680 conjugate (21839)

    Immunofluorescence with anti-Phalloidin Control, DyLight 680 conjugate (21839)

    Immunofluorescent analysis of Phalloidin (dark red) and Cytochrome c (green) in NIH 3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a Cytochrome c monoclonal antibody (Product # MA5-11823) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 680 Phalloidin (Product # 21839) at a dilution of 1:120 (2.5 units/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
     
  • IF using 21839
    Immunofluorescence with anti-Phalloidin  Control, DyLight 680 conjugate (21839)

    Immunofluorescence with anti-Phalloidin Control, DyLight 680 conjugate (21839)

    Immunofluorescent analysis of Phalloidin (dark red) and Ezrin (green) in A431 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with an Ezrin monoclonal antibody (Product # MA5-13862) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 680 Phalloidin (Product # 21839) at a dilution of 1:120 (2.5 units/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
     
  • IF using 21839
    Immunofluorescence with anti-Phalloidin  Control, DyLight 680 conjugate (21839)

    Immunofluorescence with anti-Phalloidin Control, DyLight 680 conjugate (21839)

    Immunofluorescent analysis of Phalloidin in U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 2% BSA in PBS (Product # 37525) containing 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with DyLight 680 Phalloidin (Product # 21839) or Alexa Fluor® 680 Phalloidin, each diluted to a final concentration of 2.5 units/ml in PBS, for 30 minutes. Nuclei were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Zeiss Axio Observer Z1 microscope with a 20X objective.
     
  • IF using 21840
    Immunofluorescence with anti-Phalloidin  Control, DyLight 633 conjugate (21840)

    Immunofluorescence with anti-Phalloidin Control, DyLight 633 conjugate (21840)

    Immunofluorescent analysis of Phalloidin (magenta) and alpha-Tubulin (green) in NIH 3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with an alpha-Tubulin monoclonal antibody (Product # MA1-19162) at a dilution of 1:1000 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 633 Phalloidin (Product # 21840) at a dilution of 1:120 (2.5units/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
     
  • IF using 21840
    Immunofluorescence with anti-Phalloidin  Control, DyLight 633 conjugate (21840)

    Immunofluorescence with anti-Phalloidin Control, DyLight 633 conjugate (21840)

    Immunofluorescent analysis of Phalloidin (magenta) and Cytochrome c (green) in NIH 3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a Cytochrome c monoclonal antibody (Product # MA5-11823) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 633 Phalloidin (Product # 21840) at a dilution of 1:120 (2.5units/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
     
  • IF using 21840
    Immunofluorescence with anti-Phalloidin  Control, DyLight 633 conjugate (21840)

    Immunofluorescence with anti-Phalloidin Control, DyLight 633 conjugate (21840)

    Immunofluorescent analysis of Phalloidin (magenta) and Ezrin (green) in A431 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with an Ezrin monoclonal antibody (Product # MA5-13862) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 633 Phalloidin (Product # 21840) at a dilution of 1:120 (2.5units/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
     
  • IF using 21840
    Immunofluorescence with anti-Phalloidin  Control, DyLight 633 conjugate (21840)

    Immunofluorescence with anti-Phalloidin Control, DyLight 633 conjugate (21840)

    Immunofluorescent analysis of Phalloidin (magenta) and HDAC2 (green) in A431 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with an HDAC2 polyclonal antibody (Product # PA1-861) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 633 Phalloidin (Product # 21840) at a dilution of 1:120 (2.5units/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1ug/ml for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
     
  • IF using 21840
    Immunofluorescence with anti-Phalloidin  Control, DyLight 633 conjugate (21840)

    Immunofluorescence with anti-Phalloidin Control, DyLight 633 conjugate (21840)

    Immunofluorescent analysis of Phalloidin in U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 2% BSA in PBS (Product # 37525) containing 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with DyLight 633 Phalloidin (Product # 21840) or Alexa Fluor® 633 Phalloidin, each diluted to a final concentration of 2.5 units/ml in PBS, for 30 minutes. Nuclei were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Zeiss Axio Observer Z1 microscope with a 20X objective.
     
 
 

Part of Thermo Fisher Scientific