Loading Control Antibodies

Thermo Scientific Pierce Loading Control Antibodies include antibodies against the most common loading and expression control proteins. Loading Control Antibodies are essential in the proper assessment of Western blots and are used to compare the amount of protein loaded in each well across the gel. These controls help determine whether sample-to-sample expression level differences of the protein of interest are due to actual protein levels in a given cell lysate or from loading variance in sample preparations. Pierce Loading Control Antibodies can also be used as a complementary antibody stain in immunofluorescence studies with your protein of interest. We now offer a our loading control antibodies to GAPDH, Beta Actin, and Beta Tubulin conjugated to Biotin, HRP, and our DyLight Dyes.

Antibody Target Unconjugated Biotin HRP DyLight 488 DyLight 550 DyLight 650 DyLight 680
GAPDH Loading Control Antibody (GA1R) Full-length GAPDH MA5-15738 MA5-15738-BTIN MA5-15738-HRP MA5-15738-D488 MA5-15738-D550 MA5-15738-D650 MA5-15738-D680
Β-Actin Loading Control Antibody (BA3R) Β-Actin N-terminal Peptide MA5-15739 MA5-15739-BTIN MA5-15739-HRP       MA5-15739-D680
Β-Tubulin Loading Control Antibody (BT7R) Β-Tubulin N-terminal Peptide MA5-16308 MA5-16308-BTIN MA5-16308-HRP MA5-16308-D488 MA5-16308-D550 MA5-16308-D650 MA5-16308-D680

In addition to our conjugated loading control antibodies, we offer antibodies to a variety of other common loading control proteins:

Other Loading Control Antibodies Target Product #
gamma Tubulin Loading Control Antibody (4D11) γ-Tubulin C-terminal Peptide MA1-850
Lamin A/C Loading Control Antibody (mab636) Lamin A/C MA3-1000
Cyclophilin B Loading Control Antibody Cyclophilin B C-terminal Peptide PA1-027A
Cyclophilin D Loading Control Antibody Cyclophilin D C-terminal Peptide PA3-022
COX IV Loading Control Antibody (K.473.4) COX IV N-terminal Peptide MA5-15078
Histone H3 Loading Control Antibody (E.960.2) Histone H3 C-terminal Peptide MA5-15150
Profilin 1 Loading Control Antibody Profilin 1 Synthetic Peptide PA5-17444
Beta-Gal Antibody Recombinant beta-galactosidase MA5-15222
cAMP Antibody (9H4C4) cAMP Molecule MA5-15393
E7 Antibody (3D6F1) E7 Fragment MA5-15439
Pan SUMO Antibody Pan SUMO PA5-11373
Actin Antibody (mAbGEa) Alpha-cardiac actin MA1-744
beta-Actin Antibody recombinant beta-actin PA1-183

All of these antibodies are in stock and ship within 24 hours of ordering. We also have a number of tag antibodies available in alternative species. For a complete list of alternates, search our website at www.thermoscientific.com/pierce-abs.

Protein-Protein Interaction Analysis

In addition, Thermo Scientific Protein Biology Portfolio includes products for protein-protein interaction for immunoprecipitation. Use the following links for overviews and product-related info:

Overview of Protein-Protein Interaction Analysis

Overview of Pull-Down Assays

Selection Guide for Immunoprecipitation Kits

Immunoprecipitation Kits Product Overview

IP Kits:

Argose Kits:

26146    Pierce Classic IP Kit
26147    Pierce Crosslink IP Kit
26148    Pierce Direct IP Kit
26149    Pierce Co-Immunoprecipitation Kit
20423    Pierce Protein A/G Plus Agarose
20501    AminoLink Plus Coupling Resin

Magnetic Kits:

88804    Pierce Protein A/G Magnetic IP/Co-IP Kit
88805    Pierce Crosslink Magnetic IP/Co-IP Kit

Tag Specific Kits:

HA IP/Co-IP Kits:

23610    Pierce HA-Tag IP/Co-IP Kit
88838    Pierce HA-Tag Magnetic IP/Co-IP Kit

c-Myc IP/Co-IP Kit:

23620    Pierce c-Myc-Tag IP/Co-IP Kit (Product # 23620)

GST Pull-Down Kit:

21516    Pierce GST Protein Interaction Pull-Down Kit (Product # 21516)
16100    Pierce Glutathione Agarose (Product # 16100)

His Pull-Down Kit:

21277    Pierce His Protein Interaction Pull-Down Kit (Product # 21277)
88221    HisPur Ni-NTA Resin (Product # 88221)
89964    HisPur Cobalt Resin (Product # 89964)

Part of Thermo Fisher Scientific