p53 Antibody (DO-7)

p53 Monoclonal Antibody for Western blot, IF, ICC, IHC (P), FACS, IP

>> See 39 other antibodies for TP53 (antigen NY-CO-13, cellular tumor antigen p53, p53 tumor suppressor, phosphoprotein p53, transformation-related protein 53, TP53)
Synonyms:
antigen NY-CO-13, cellular tumor antigen p53, p53 tumor suppressor, phosphoprotein p53, transformation-related protein 53, TP53
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Mouse / IgG2b, kappa
Class: Monoclonal
Type: Antibody
Clone: DO-7
Tested Species Reactivity: Human (Hu)
Published Species Reactivity: Human (Hu), Mouse (Ms)
Immunogen: Recombinant human wild-type p53 protein expressed in E. coli
Ordering Information
Pierce p53 Antibody (DO-7)
Product #
MA5-12557
Size
500 ul
Price
$299.00
Purchase
Add p53 Antibody (DO-7) to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:50-1:200
Immunofluorescence (IF) 1:20-1:200
Immunocytochemistry (ICC) 1:20-1:200
Immunohistochemistry (Paraffin) (IHC (P)) 1:100 - 200
Flow Cytometry (FACS) Assay Dependent
Immunoprecipitation (IP) 1:50-1:100
Published Applications Dilution
Western Blot (WB) See publications below
Immunohistochemistry (IHC) See publications below
Immunoprecipitation (IP) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 0.1mg/ml
Purification: Protein A
Storage Buffer: PBS, pH 7.4, with 0.2% BSA
Preservative: 0.09% sodium azide
Storage Conditions: 4° C
Product Specific Information
MA5-12557 targets p53 in FACS, ICC/IF, IHC (P), IP, and WB applications and shows reactivity with Human samples. This antibody is not suitable for mouse colon tissue in IHC applications.

The MA5-12557 immunogen is recombinant human wild-type p53 protein expressed in E. coli.

This antibody was orginally validated as part of a Thermo Scientific Cellomics High Content Screening Kit. The antibody sold separately may have slightly different performance and may need to be further optimized for the best results.
General Information
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons
Product Images
  • p53 Antibody (MA5-12557) in WB
    MA5-12557_WesternBlot.jpg

    Western Blot with anti-p53 Monoclonal Antibody [DO-7] (MA5-12557) (MA5-12557_WesternBlot.jpg)

    Western Blot with anti-p53 Monoclonal Antibody [DO-7] (MA5-12557)

    Western blot analysis of p53 was performed by loading 50ug of SKBR3 human breast cancer whole cell lysate per well onto a SDS-PAGE gel. Proteins were transferred to a membrane and blocked with 5% nonfat dry milk in PBS-0.1% Tween-20 for 30 minutes. The membrane was probed with a p53 monoclonal antibody (Product # MA5-12557) at a concentration of 1ug/ml overnight at 4°C on a rocking platform, washed in PBS-0.1%Tween-20, and probed with an anti-mouse IgG-HRP secondary antibody for 1 hour. Detection was performed using a chemiluminescent substrate..
  • p53 Antibody (MA5-12557) in WB
    MA5-12557_Westerblot_20140415143844.jpg

    Western Blot with anti-p53 Monoclonal Antibody [DO-7] (MA5-12557) (MA5-12557_Westerblot_20140415143844.jpg)

    Western Blot with anti-p53 Monoclonal Antibody [DO-7] (MA5-12557)

    Western blot analysis of p53 was performed by loading 25 ug of 293 (Lane 1) and A431 (Lane 2) cell lysates and a molecular weight protein ladder onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with a blocking buffer at 4ºC overnight. The membrane was probed with a p53 monoclonal antibody (Product # MA5-12557) at a dilution of 1:200 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Results show a band at 53 kDa.
  • p53 Antibody (MA5-12557) in IF
    p53-mouse_MA5-12557_Immunoflourescence.jpg

    Immunofluorescence with anti-p53 Monoclonal Antibody [DO-7] (MA5-12557) (p53-mouse_MA5-12557_Immunoflourescence.jpg)

    Immunofluorescence with anti-p53 Monoclonal Antibody [DO-7] (MA5-12557)

    Activation of ATM and p53: ATM kinase is auto-phosphorylated in response to DNA damage and is involved in DNA damage induced activation of p53 and cell cycle arrest. This is a multiplex kit, enabling the user to simultaneously measure both phospho-ATM and p53. A549 cells were treated with vehicle (DMSO) or 50 µM etoposide for 24 hrs. After staining cells with Cellomics phospho-ATM/p53 duplex activation kit, images were acquired and analyzed. Z-factor for phospho-ATM = 0.46 ± 0.11 and %COV = 11 ± 3. Z-factor for p53 = 0.44 ± 0.03 and %COV = 13 ± 1. EC50 of etoposide treatment for ATM activation is 19 µM and EC50 for p53 activation is 36 µM. Etoposide is a topoisomerase II inhibitor resulting in DNA strand break.
  • p53 Antibody (MA5-12557) in IF
    p53_Antibody_MA5-12557_Immunofluorescence_1.jpg

    Immunofluorescence with anti-p53 Monoclonal Antibody [DO-7] (MA5-12557) (p53_Antibody_MA5-12557_Immunofluorescence_1.jpg)

    Immunofluorescence with anti-p53 Monoclonal Antibody [DO-7] (MA5-12557)

    Immunofluorescent analysis of p53 (green) in HeLa cells either left untreated (left panel) or treated with 5uM Camptothecin (right panel) for 20 hours. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a p53 monoclonal antibody (Product # MA5-12557) at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin (Product # 21834) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan or ToxInsight Instrument at 20X magnification.
  • p53 Antibody (MA5-12557) in IF
    MA5-12557_IF_A431_20130724074740.jpg

    Immunofluorescence with anti-p53 Monoclonal Antibody [DO-7] (MA5-12557) (MA5-12557_IF_A431_20130724074740.jpg)

    Immunofluorescence with anti-p53 Monoclonal Antibody [DO-7] (MA5-12557)

    Immunofluorescent analysis of p53 (green) showing staining in the nucleus of A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a p53 monoclonal antibody (Product # MA5-12557) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
  • p53 Antibody (MA5-12557) in IF
    MA5-12557_IF_HT29_20130724074759.jpg

    Immunofluorescence with anti-p53 Monoclonal Antibody [DO-7] (MA5-12557) (MA5-12557_IF_HT29_20130724074759.jpg)

    Immunofluorescence with anti-p53 Monoclonal Antibody [DO-7] (MA5-12557)

    Immunofluorescent analysis of p53 (green) showing staining in the nucleus of HT29 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a p53 monoclonal antibody (Product # MA5-12557) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
  • p53 Antibody (MA5-12557) in IHC
    MA5-12557_Immunohistochemistry.jpg

    Immunohistochemistry with anti-p53 Monoclonal Antibody [DO-7] (MA5-12557) (MA5-12557_Immunohistochemistry.jpg)

    Immunohistochemistry with anti-p53 Monoclonal Antibody [DO-7] (MA5-12557)

    Immunohistochemistry was performed on formalin-fixed, paraffin-embedded human colon carcinoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer for 20 minutes at 95°C. Following antigen retrieval tissues were blocked and probed with a p53 monoclonal antibody (Product # MA5-12557) at a concentration of 1ug/ml for 30 minutes at room temp. Detection was performed using a HRP-conjugated secondary reagent followed by a colorimetric substrate.
  • p53 Antibody (MA5-12557) in IHC (P)
    MA5-12557_IHC_human colon carcinoma.jpg

    Immunohistochemistry (Paraffin) with anti-p53 Monoclonal Antibody [DO-7] (MA5-12557) (MA5-12557_IHC_human colon carcinoma.jpg)

    Immunohistochemistry (Paraffin) with anti-p53 Monoclonal Antibody [DO-7] (MA5-12557)

    Immunohistochemistry analysis of p53 showing positive staining in the nucleus and cytoplasm of paraffin-treated Human colon carcinoma (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a p53 monoclonal antibody (Product # MA5-12557) diluted by 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
  • p53 Antibody (MA5-12557) in IHC (P)
    MA5-12557_IHC_human breast carcinoma.jpg

    Immunohistochemistry (Paraffin) with anti-p53 Monoclonal Antibody [DO-7] (MA5-12557) (MA5-12557_IHC_human breast carcinoma.jpg)

    Immunohistochemistry (Paraffin) with anti-p53 Monoclonal Antibody [DO-7] (MA5-12557)

    Immunohistochemistry analysis of p53 showing positive staining in the nucleus and cytoplasm of paraffin-treated Human breast carcinoma (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a p53 monoclonal antibody (Product # MA5-12557) diluted by 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
  • p53 Antibody (MA5-12557) in FACS
    MA5-12557_FACS_CEM.jpg

    Flow Cytometry with anti-p53 Monoclonal Antibody [DO-7] (MA5-12557) (MA5-12557_FACS_CEM.jpg)

    Flow Cytometry with anti-p53 Monoclonal Antibody [DO-7] (MA5-12557)

    Flow cytometry analysis of p53 in CEM cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde, washed with PBS, and incubated with p53 monoclonal antibody (Product # MA5-12557) at a dilution of 1 ug/test for 60 min at room temperature. Cells were then blocked in a solution of 2% BSA-PBS for 30 min at room temperature, incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody, and re-suspended in PBS for FACS analysis.
  • p53 Antibody (MA5-12557) in FACS
    MA5-12557_FACS_A431.jpg

    Flow Cytometry with anti-p53 Monoclonal Antibody [DO-7] (MA5-12557) (MA5-12557_FACS_A431.jpg)

    Flow Cytometry with anti-p53 Monoclonal Antibody [DO-7] (MA5-12557)

    Flow cytometry analysis of p53 in A431 cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde, washed with PBS, and incubated with p53 monoclonal antibody (Product # MA5-12557) at a dilution of 0.5 ug/test for 60 min at room temperature. Cells were then blocked in a solution of 2% BSA-PBS for 30 min at room temperature, incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody, and re-suspended in PBS for FACS analysis.
  • p53 Antibody (MA5-12557) in FACS
    MA5-12557_FACS_BAEC.jpg

    Flow Cytometry with anti-p53 Monoclonal Antibody [DO-7] (MA5-12557) (MA5-12557_FACS_BAEC.jpg)

    Flow Cytometry with anti-p53 Monoclonal Antibody [DO-7] (MA5-12557)

    Flow cytometry analysis of p53 in BAEC cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde, washed with PBS, and incubated with p53 monoclonal antibody (Product # MA5-12557) at a dilution of 0.5 ug/test for 60 min at room temperature. Cells were then blocked in a solution of 2% BSA-PBS for 30 min at room temperature, incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody, and re-suspended in PBS for FACS analysis.
  • p53 Antibody (MA5-12557) in IP
    MA5-12557_Immunoprecipitation.jpg

    Immunoprecipitation with anti-p53 Monoclonal Antibody [DO-7] (MA5-12557) (MA5-12557_Immunoprecipitation.jpg)

    Immunoprecipitation with anti-p53 Monoclonal Antibody [DO-7] (MA5-12557)

    Immunoprecipitation of p53 using p53 Monoclonal Antibody (MA5-12557) on denatured Human MDA231 Cells.
Publications:
Western Blot
  Species / Dilution Summary
  Hu / Not Cited
MA5-12557 was used in immunoprecipitation and western blot to investigate HCCR-2 expression in human cancers and its role in tumorigenesis

Oncogene. 2003 Jul 24;22(30):4679-89.
"Identification and differential expression of novel human cervical cancer oncogene HCCR-2 in human cancers and its involvement in p53 stabilization."
Author(s): Ko J, Lee YH, Hwang SY, Lee YS, Shin SM, Hwang JH, Kim J, Kim YW, Jang SW, Ryoo ZY, Kim IK, Namkoong SE, Kim JW
Number of Citations: 1
(See PubMed article )
  Hu / 0
MA5-12557 was used in immunohistochemistry and western blot to investigate the influence of human papillomavirus 16 infection on cellular immune response to squamous cell carcinoma of the head and neck

Clin Cancer Res. 2004 Oct 15;10(20):6929-37.
"Effect of human papillomavirus-16 infection on CD8+ T-cell recognition of a wild-type sequence p53264-272 peptide in patients with squamous cell carcinoma of the head and neck."
Author(s): Sirianni N, Ha PK, Oelke M, Califano J, Gooding W, Westra W, Whiteside TL, Koch WM, Schneck JP, DeLeo A, Ferris RL
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
MA5-12557 was used in western blot to investigate the role of CDK2 in the MYCN over-expressing cancer cells

Proc Natl Acad Sci U S A. 2009 Aug 4;106(31):12968-73.
"Inactivation of CDK2 is synthetically lethal to MYCN over-expressing cancer cells."
Author(s): Molenaar JJ, Ebus ME, Geerts D, Koster J, Lamers F, Valentijn LJ, Westerhout EM, Versteeg R, Caron HN
Number of Citations: 7
(See PubMed article )
  Hu / Not Cited
MA5-12557 was used in immunohistochemistry and western blot to study the role of tumor suppressors p53 and p21(WAF1/CIP1) in mammary epithelial cell proliferaion

J Cell Sci. 2009 Aug 15;122(Pt 16):2989-95.
"Normal human mammary epithelial cells proliferate rapidly in the presence of elevated levels of the tumor suppressors p53 and p21(WAF1/CIP1)."
Author(s): Huschtscha LI, Moore JD, Noble JR, Campbell HG, Royds JA, Braithwaite AW, Reddel RR
Number of Citations: 1
(See PubMed article )
  Ms / 1:1000
MA5-12557 was used in western blot to investigate the effect of glutathione on chromosome aberrations, cell cycle kinetics, and cell cycle regulator proteins

Int J Radiat Biol. 2007 May;83(5):347-54.
"Influence of glutathione on the induction of chromosome aberrations, delay in cell cycle kinetics and cell cycle regulator proteins in irradiated mouse bone marrow cells."
Author(s): Ray S, Chatterjee A
Number of Citations: 1
(See PubMed article )
Immunohistochemistry
  Species / Dilution Summary
  Hu / 1:100
MA5-12557 was used in immunohistochemistry to investigate the correlation of p53 and Ki-67 with the recurrence and progression of superficial urothelial cell carcinomas of urinary bladder

Pathol Int. 2002 Jul;52(7):463-9.
"Impact of p53 and Ki-67 in predicting recurrence and progression of superficial (pTa and pT1) urothelial cell carcinomas of urinary bladder."
Author(s): Kilicli-Camur N, Kilicaslan I, Gulluoglu MG, Esen T, Uysal V
Number of Citations: 0
(See PubMed article )
  Hu / 1:200
MA5-12557 was used in immunohistochemistry to investigate the beta-catenin pathway in non-melanoma skin tumors

Am J Pathol. 2003 Dec;163(6):2277-87.
"Alterations of beta-catenin pathway in non-melanoma skin tumors: loss of alpha-ABC nuclear reactivity correlates with the presence of beta-catenin gene mutation."
Author(s): Doglioni C, Piccinin S, Demontis S, Cangi MG, Pecciarini L, Chiarelli C, Armellin M, Vukosavljevic T, Boiocchi M, Maestro R
Number of Citations: 1
(See PubMed article )
  Hu / 0
MA5-12557 was used in immunohistochemistry to study the molecular determinants of the response to radiotherapy in oral carcinoma

Int J Cancer. 2004 May 1;109(5):710-6.
"p53, p16 and cyclin D1: molecular determinants of radiotherapy treatment response in oral carcinoma."
Author(s): Jayasurya R, Francis G, Kannan S, Lekshminarayanan K, Nalinakumari KR, Abraham T, Abraham EK, Nair MK
Number of Citations: 2
(See PubMed article )
  Hu / 1:100
MA5-12557 was used in immunohistochemistry to report on a case of paratesticular clear cell carcinoma of the tunica vaginalis of the testis

Pathol Int. 2004 Aug;54(8):641-7.
"Clear cell adenocarcinoma of the tunica vaginalis of the testis with an adjacent uterus-like tissue."
Author(s): Tulunay O, Gögü? C, Baltaci S, Bulut S
Number of Citations: 0
(See PubMed article )
  Hu / 0
MA5-12557 was used in immunohistochemistry and western blot to investigate the influence of human papillomavirus 16 infection on cellular immune response to squamous cell carcinoma of the head and neck

Clin Cancer Res. 2004 Oct 15;10(20):6929-37.
"Effect of human papillomavirus-16 infection on CD8+ T-cell recognition of a wild-type sequence p53264-272 peptide in patients with squamous cell carcinoma of the head and neck."
Author(s): Sirianni N, Ha PK, Oelke M, Califano J, Gooding W, Westra W, Whiteside TL, Koch WM, Schneck JP, DeLeo A, Ferris RL
Number of Citations: 1
(See PubMed article )
  Hu / 0
MA5-12557 was used in immunohistochemistry to investigate the prognostic significance of p21waf1/cip1 expression in oral squamous cell carcinoma

J Oral Pathol Med. 2005 May;34(5):274-9.
"p21WAF1/CIP1 expression is a marker of poor prognosis in oral squamous cell carcinoma."
Author(s): Nemes JA, Nemes Z, Márton IJ
Number of Citations: 1
(See PubMed article )
  Hu / 1:100
MA5-12557 was used in immunohistochemistry to study the role of Ki-67, p53, estrogen and progesterone receptors in uterine leiomyosarcomas

Gynecol Oncol. 2005 Oct;99(1):36-42.
"The expression of Ki-67, p53, estrogen and progesterone receptors affecting survival in uterine leiomyosarcomas. A clinicopathologic study."
Author(s): Akhan SE, Yavuz E, Tecer A, Iyibozkurt CA, Topuz S, Tuzlali S, Bengisu E, Berkman S
Number of Citations: 5
(See PubMed article )
  Hu / 1:50
MA5-12557 was used in immunohistochemistry to evaluate the prognostic value of p53 expression in stage III non-small cell lung cancer

Anticancer Res. 2005 May-Jun;25(3c):2385-9.
"Prognostic role of p53 in stage III non-small cell lung cancer."
Author(s): Berghmans T, Mascaux C, Martin B, Ninane V, Sculier JP
Number of Citations: 1
(See PubMed article )
  Hu / 1:100
MA5-12557 was used in immunohistochemistry The report on a patient with pulmonary large cell carcinoma with rhabdoid phenotype

Ann Diagn Pathol. 2005 Aug;9(4):223-6.
"Pulmonary large cell carcinoma with rhabdoid phenotype."
Author(s): Yilmazbayhan D, Ates LE, Dilege S, Gulluoglu M, Tanju S, Kalayci G
Number of Citations: 0
(See PubMed article )
  Hu / 2 ug/ml
MA5-12557 was used in immunohistochemistry to investigate the presence and utility of p53 antibodies in patients with breast cancer

Breast Cancer Res Treat. 2005 Sep;93(2):111-5.
"The presence of serum anti-p53 antibodies from patients with invasive ductal carcinoma of breast: correlation to other clinical and biological parameters."
Author(s): Gao RJ, Bao HZ, Yang Q, Cong Q, Song JN, Wang L
Number of Citations: 1
(See PubMed article )
  Hu / 0
MA5-12557 was used in immunohistochemistry to study the role of survivin in pre-invasive lesions and non-small cell lung carcinoma

Virchows Arch. 2006 Aug;449(2):164-70.
"Survivin expression in pre-invasive lesions and non-small cell lung carcinoma."
Author(s): Akyürek N, Memi? L, Ekinci O, Köktürk N, Oztürk C
Number of Citations: 6
(See PubMed article )
  Hu / 0
MA5-12557 was used in immunohistochemistry to report on a case of myointimoma of the glans penis

Pathol Int. 2007 Mar;57(3):158-61.
"Myointimoma of the glans penis."
Author(s): Vardar E, Gunlusoy B, Arslan M, Kececi S
Number of Citations: 0
(See PubMed article )
  Hu / 0
MA5-12557 was used in immunohistochemistry to study potential prognostic markers in Hungarian breast cancer patients

Anticancer Res. 2007 Jan-Feb;27(1A):279-82.
"Prognostic factors in Hungarian breast cancer patients."
Author(s): Nádasi E, Anga B, Sándor J, Megyesi J, Kelemen D, Mottolese M, Natali PG, Hegedus G, Arany I, Ember I
Number of Citations: 0
(See PubMed article )
  Hu / 1:50
MA5-12557 was used in immunohistochemistry to report on a morphological and molecular analysis of two cases of Villoglandular adenocarcinoma of the cervix

Int J Gynecol Pathol. 2007 Apr;26(2):199-204.
"Villoglandular adenocarcinoma of the cervix: two new cases with morphological and molecular study."
Author(s): Giordano G, D'Adda T, Gnetti L, Merisio C, Gabrielli M, Melpignano M
Number of Citations: 2
(See PubMed article )
  Hu / Not Cited
MA5-12557 was used in immunohistochemistry to investigate the efficacy of p53 therapy in Li-Fraumeni syndrome

Mol Cancer Ther. 2007 May;6(5):1478-82.
"p53 therapy in a patient with Li-Fraumeni syndrome."
Author(s): Senzer N, Nemunaitis J, Nemunaitis M, Lamont J, Gore M, Gabra H, Eeles R, Sodha N, Lynch FJ, Zumstein LA, Menander KB, Sobol RE, Chada S
Number of Citations: 4
(See PubMed article )
  Hu / 1:25
MA5-12557 was used in immunohistochemistry to report on a case of transitional cell carcinoma of the endometrium associated with benign ovarian brenner tumor

Int J Gynecol Pathol. 2007 Jul;26(3):298-304.
"Transitional cell carcinoma of the endometrium associated with benign ovarian brenner tumor: a case report with immunohistochemistry molecular analysis and a review of the literature."
Author(s): Giordano G, D'Adda T, Gnetti L, Merisio C, Raboni S
Number of Citations: 0
(See PubMed article )
  Hu / 1:200
MA5-12557 was used in immunohistochemistry to study the diagnostic and prognostic value of cell cycle regulators in differentiated thyroid cancer

Ann Surg Oncol. 2007 Dec;14(12):3403-11.
"Cell cycle regulators show diagnostic and prognostic utility for differentiated thyroid cancer."
Author(s): Melck A, Masoudi H, Griffith OL, Rajput A, Wilkins G, Bugis S, Jones SJ, Wiseman SM
Number of Citations: 3
(See PubMed article )
  Hu / 0
MA5-12557 was used in immunohistochemistry to study the prognostic value of different PIK3CA gene mutations in breast cancer

Clin Cancer Res. 2007 Oct 15;13(20):6064-9.
"Different prognostic roles of mutations in the helical and kinase domains of the PIK3CA gene in breast carcinomas."
Author(s): Barbareschi M, Buttitta F, Felicioni L, Cotrupi S, Barassi F, Del Grammastro M, Ferro A, Dalla Palma P, Galligioni E, Marchetti A
Number of Citations: 20
(See PubMed article )
  Hu / 0
MA5-12557 was used in immunohistochemistry to perform immunohistochemical studies of apocrine carcinomas in Turkish women with breast cancer

Pathol Res Pract. 2008;204(6):367-71.
"Apocrine carcinomas of the breast in Turkish women: hormone receptors, c-erbB-2 and p53 immunoexpression."
Author(s): Kaya H, Bozkurt SU, Erbarut I, Djamgoz MB
Number of Citations: 1
(See PubMed article )
  Hu / 1:50
MA5-12557 was used in immunohistochemistry to examine the diagnostic significance of p53 protein in the tumorigenesis in biliopancreatic tree

Dig Dis Sci. 2009 Apr;54(4):789-92.
"Immunocytochemical assessment of p53 protein to detect malignancy in increased cell-yield brush cytology from the biliopancreatic tree."
Author(s): Villanacci V, Cestari R, Giulini S, Cengia P, Missale G, Berenzi A, Rossi E, Bonardi M, Baiocchi L, Bassotti G
Number of Citations: 1
(See PubMed article )
  Hu / 1:50
MA5-12557 was used in immunohistochemistry to investigate the expression of some histopathological and immunohistochemical markers in sudden cardiac death

Rom J Morphol Embryol. 2008;49(3):315-20.
"Immunophenotypical pleomorphism expression in sudden cardiac death."
Author(s): Ceau?u M, Curc? C, Ardeleanu C, Dermengiu D
Number of Citations: 0
(See PubMed article )
  Hu / Not Cited
MA5-12557 was used in immunohistochemistry to investigate the expression of mammalian actin regulatory protein Ena in colorectal lesions

Rom J Morphol Embryol. 2008;49(3):345-9.
"The expression of cytoskeleton regulatory protein Mena in colorectal lesions."
Author(s): Gurzu S, Jung I, Prantner I, Ember I, Pávai Z, Mezei T
Number of Citations: 7
(See PubMed article )
  Hu / 0
MA5-12557 was used in immunohistochemistry to investigate the gene events of p16INK4a and p14ARF in oral proliferative verrucous leukoplakia and their correlation with disease progression and prognosis

Cancer Epidemiol Biomarkers Prev. 2008 Nov;17(11):3179-87.
"Frequent alterations of p16INK4a and p14ARF in oral proliferative verrucous leukoplakia."
Author(s): Kresty LA, Mallery SR, Knobloch TJ, Li J, Lloyd M, Casto BC, Weghorst CM
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
MA5-12557 was used in immunohistochemistry to examine the prognostic significance of multiple genes in osteosarcoma

Acta Orthop Traumatol Turc. 2009 Jan-Feb;43(1):28-34.
"[The effect of resistance-related proteins on the prognosis and survival of patients with osteosarcoma: an immunohistochemical analysis]."
Author(s): Ozger H, Eralp L, Atalar AC, Toker B, Esberk Ate? L, Sungur M, Bilgiç B, Ayan I
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
MA5-12557 was used in immunohistochemistry and western blot to study the role of tumor suppressors p53 and p21(WAF1/CIP1) in mammary epithelial cell proliferaion

J Cell Sci. 2009 Aug 15;122(Pt 16):2989-95.
"Normal human mammary epithelial cells proliferate rapidly in the presence of elevated levels of the tumor suppressors p53 and p21(WAF1/CIP1)."
Author(s): Huschtscha LI, Moore JD, Noble JR, Campbell HG, Royds JA, Braithwaite AW, Reddel RR
Number of Citations: 1
(See PubMed article )
  Hu / 1:200
MA5-12557 was used in immunohistochemistry to investigate the association of Axin expression with non-small-cell lung cancer apoptosis induced by X-radiation

Int J Radiat Oncol Biol Phys. 2009 Oct 1;75(2):518-26.
"X-radiation induces non-small-cell lung cancer apoptosis by upregulation of Axin expression."
Author(s): Han Y, Wang Y, Xu HT, Yang LH, Wei Q, Liu Y, Zhang Y, Zhao Y, Dai SD, Miao Y, Yu JH, Zhang JY, Li G, Yuan XM, Wang EH
Number of Citations: 3
(See PubMed article )
  Hu / 1:100
MA5-12557 was used in immunohistochemistry to investigate the expression of specific genes in resected adenocarcinoma of lung

Cancer. 2010 Feb 1;116(3):676-85.
"Clinical impact of phosphorylated signal transducer and activator of transcription 3, epidermal growth factor receptor, p53, and vascular endothelial growth factor receptor 1 expression in resected adenocarcinoma of lung by using tissue microarray."
Author(s): Kim HS, Park YH, Lee J, Ahn JS, Kim J, Shim YM, Kim JH, Park K, Han J, Ahn MJ
Number of Citations: 1
(See PubMed article )
  Hu / 1:100
MA5-12557 was used in immunohistochemistry to compare expression of Maspin, VEGF, and p53 between carcinomas

Pathol Oncol Res. 2010 Dec;16(4):553-61.
"Maspin, VEGF and p53 expression in small biopsies of primary advanced lung cancer and relationship with clinicopathologic parameters."
Author(s): Bircan A, Bircan S, Kapucuoglu N, Songur N, Ozturk O, Akkaya A
Number of Citations: 0
(See PubMed article )
  Hu / 1:1000
MA5-12557 was used in immunohistochemistry to investigate the relationship of p53 expression with DNA aneuploidy in vulva malignancies

Hum Pathol. 2010 Oct;41(10):1475-85.
"High levels of p53 expression correlate with DNA aneuploidy in (pre)malignancies of the vulva."
Author(s): van der Avoort IA, van de Nieuwenhof HP, Otte-Höller I, Nirmala E, Bulten J, Massuger LF, van der Laak JA, Slootweg PJ, de Hullu JA, van Kempen LC
Number of Citations: 1
(See PubMed article )
  Hu / 1:100
MA5-12557 was used in immunohistochemistry to report on a case of papillary carcinoma with diffuse papillomatosis of the gallbladder and cystic duct

Ann Diagn Pathol. 2011 Apr;15(2):140-4.
"Papillary carcinoma with diffuse papillomatosis of gallbladder and cystic duct."
Author(s): Kosemehmetoglu K, Akpinar E, Sokmensuer C, Hamaloglu E
Number of Citations: 0
(See PubMed article )
  Hu / 1:500
MA5-12557 was used in immunohistochemistry to investigate the secretion of anti-diuretic hormone in neuroblastoma

Endocr Pathol. 2010 Dec;21(4):266-73.
"Primary sellar neuroblastoma presenting with syndrome of inappropriate secretion of anti-diuretic hormone."
Author(s): Radotra B, Apostolopoulos V, Sandison A, Hatfield EC, Mendoza N, Moss J, Mehta A, Glaser M, Meeran K, Roncaroli F
Number of Citations: 1
(See PubMed article )
  Hu / 1:300
MA5-12557 was used in immunohistochemistry to investigate the effect of galectin-3 and heparanase on the pathogenesis of endometrial carcinomas

Arch Gynecol Obstet. 2011 Nov;284(5):1231-9.
"Investigation of galectin-3 and heparanase in endometrioid and serous carcinomas of the endometrium and correlation with known predictors of survival."
Author(s): Ege CB, Akbulut M, Zekio?lu O, Ozdemir N
Number of Citations: 1
(See PubMed article )
  Hu / 0
MA5-12557 was used in immunohistochemistry to investigate esophageal function and its relationship with gastroesophageal reflux disease and Barrett esophagus in patients with cervical inlet patch

Dis Esophagus. 2011 Nov 22;():.
"Detailed esophageal function and morphological analysis shows high prevalence of gastroesophageal reflux disease and Barrett's esophagus in patients with cervical inlet patch."
Author(s): Rosztóczy A, Izbéki F, Németh IB, Dulic S, Vadászi K, Róka R, Gecse K, Gyökeres T, Lázár G, Tiszlavicz L, Wittmann T
Number of Citations: 1
(See PubMed article )
  Hu / 1:1500
MA5-12557 was used in immunohistochemistry to study a case of primary squamous cell carcinoma of the thyroid

Pathol Int. 2012 Jan;62(1):43-8.
"Primary pure squamous cell carcinoma of the thyroid: report and histogenic consideration of a case involving a BRAF mutation."
Author(s): Ko YS, Hwang TS, Han HS, Lim SD, Kim WS, Oh SY
Number of Citations: 1
(See PubMed article )
  Hu / 1:250
MA5-12557 was used in immunohistochemistry to investigate the relation between endometrial intraepithelial carcinoma and serous ovarian carcinoma

Int J Gynecol Cancer. 2012 Mar;22(3):457-64.
"Concurrent endometrial intraepithelial carcinoma (EIC) and serous ovarian cancer: can EIC be seen as the precursor lesion?"
Author(s): Roelofsen T, van Kempen LC, van der Laak JA, van Ham MA, Bulten J, Massuger LF
Number of Citations: 1
(See PubMed article )
Immunoprecipitation
  Species / Dilution Summary
  Hu / Not Cited
MA5-12557 was used in immunoprecipitation and western blot to investigate HCCR-2 expression in human cancers and its role in tumorigenesis

Oncogene. 2003 Jul 24;22(30):4679-89.
"Identification and differential expression of novel human cervical cancer oncogene HCCR-2 in human cancers and its involvement in p53 stabilization."
Author(s): Ko J, Lee YH, Hwang SY, Lee YS, Shin SM, Hwang JH, Kim J, Kim YW, Jang SW, Ryoo ZY, Kim IK, Namkoong SE, Kim JW
Number of Citations: 1
(See PubMed article )
  Hu / 2 ug/ml
MA5-12557 was used in immunoprecipitation to study the role of the p53 target gene FLJ11259/DRAM encoding a novel transmembrane protein

Biochim Biophys Acta. 2007 Apr;1769(4):209-19.
"The direct p53 target gene, FLJ11259/DRAM, is a member of a novel family of transmembrane proteins."
Author(s): Kerley-Hamilton JS, Pike AM, Hutchinson JA, Freemantle SJ, Spinella MJ
Number of Citations: 6
(See PubMed article )
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