gamma ENaC Antibody

gamma ENaC Polyclonal Antibody for IP

>> See 1 other antibody for Scnn1g (Epithelial Sodium Channel Gamma)
Synonyms:
Epithelial Sodium Channel Gamma
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Rabbit / IgG
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Rat (Rt)
Published Species Reactivity: Amphibian (Am), Human (Hu), Non-human primate (Nhp)
Immunogen: Synthetic peptide corresponding to residues L(630) R L E R A F S N Q L T D T Q M L D E L(649) of rat gamma-ENaC.
Ordering Information
Pierce gamma ENaC Antibody
Product #
PA1-922
Size
100 µg
Price
$360.00
Purchase
Add gamma ENaC Antibody to your cart.
Tested Applications Dilution *
Immunoprecipitation (IP) Assay dependent
Published Applications Dilution
Western Blot (WB) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 1mg/ml
Purification: Antigen affinity chromatography
Storage Buffer: PBS with 1mg/ml BSA
Preservative: 0.05% sodium azide
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
PA1-922 detects recombinant rat gamma-epithelial sodium channel (gamma-ENaC).

PA1-922 has been successfully used in immunoprecipitation procedures. By immunoprecipitation this antibody specifically binds to the unglycosylated, ~75 kDa recombinant rat gamma-ENaC protein.

This product fails to detect gamma-ENaC protein in Western blot procedures.

PA1-922 immunizing peptide corresponds to amino acid residues 630-649 from human gamma-ENaC. This sequence is 76%, 71%, and 66% conserved in rat, mouse, and rabbit gamma-ENaC, respectively. PA1-922 immunizing peptide (Cat. # PEP-090) is available for use in neutralization and control experiments.
General Information
Epithelial sodium channels are amiloride-sensitive members of the degenerin/epithelial sodium channel (Deg/ENaC) superfamily of ion channels. Members of this superfamily of ion channels share organizational similarity in that they all possess two short intracellular amino and carboxyl termini, two short membrane spanning segments, and a large extracellular loop with a conserved cysteine-rich region. There are three homologous isoforms of the ENaC (alpha, beta, and gamma) protein. ENaC in the kidney, lung, and colon plays an essential role in trans-epithelial sodium and fluid balance. ENaC also mediates aldosterone-dependent sodium reabsorption in the distal nephron of the kidney, thus regulating blood pressure. ENaC is thought to be regulated, in part, through association with the cystic fibrosis transmembrane conductance regulator (CFTR) chloride ion channel. Gain-of-function mutations in beta- or gamma-ENaC can cause severe arterial hypertension (Liddel’s syndrome) and loss-of-function mutations in alpha- or beta-ENaC causes pseudohypoaldosteronism (PHA-1).
Publications:
Western Blot
  Species / Dilution Summary
  Am / Not Cited
PA1-922 was used in western blot to investigate the role of the trypsin during the activation of the epithelial sodium channel.

J Biol Chem. 2007 Sep 14;282(37):26884-96.
"Indirect activation of the epithelial Na+ channel by trypsin."
Author(s): Bengrine A, Li J, Hamm LL, Awayda MS
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
Nhp / Not Cited
PA1-922 was used in western blot to investigate the interactions between gamaENaC and other subunits of human epithelial sodium channels.

J Biol Chem. 2006 Mar 24;281(12):8233-41.
"Delta-subunit confers novel biophysical features to alpha beta gamma-human epithelial sodium channel (ENaC) via a physical interaction."
Author(s): Ji HL, Su XF, Kedar S, Li J, Barbry P, Smith PR, Matalon S, Benos DJ
Number of Citations: 1
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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