Full-service, customized production of monoclonal and polyclonal antibodies.
Thermo Scientific Pierce Custom Antibody Services include many competitively-priced packages and specialized purification options for antibody production, all supported by powerful online tools for peptide design and project tracking.
Our capabilities enable us to provide complete support for all types and steps of antibody production: from peptide design, synthesis and carrier protein conjugation to animal immunization, serum collection or hybridoma fusion, titer analysis, and final antibody purification. We specialize in antigen design and generation of custom peptide antibodies and monospecific peptide antibodies to highly discrete epitopes. We also immunize with recombinant proteins or protein fragments provided by you (or expressed and purified in our lab using cDNAs from our extensive in-house genomics library) to generate antibodies in rabbits, chickens, goats, guinea pigs, mice or rats. Our detailed knowledge of antigen determining factors (e.g., size, localized secondary structure and antigenicity) allow us to produce custom antibodies with unmatched specificity, affinity and assay utility. This expert service increases the probability that you will obtain a polyclonal or monoclonal antibody capable of distinguishing among even highly related proteins within complex mixtures to characterize protein expression patterns using IHC, ICC, IF, FACS, ELISA, Western blotting or immunoprecipitation (IP, Co-IP, ChIP).
Highlights:
- Peptide design, synthesis and conjugation – we will help you design the best possible antigenic peptide using our exclusive Thermo Scientific Antigen Profiler System; then we'll synthesize the peptide for you and conjugate it to the carrier protein of your choice.
- Fusion protein expression and purification – we offer bacterial, mammalian and insect protein expression services as part of our antibody development platform; we'll make the your protein and purify it to prepare it for immunization and ELISA testing.
- Polyclonal or monoclonal antibody protocols - choose one of six species options (eight immunization schedules) for polyclonal antibody production or one of three package sizes (number and strains of mice) for monoclonal antibody production.
- Biomarker antibody development - choose from several standardized packages for producing polyclonal or monoclonal antibodies to specific biomarkers of interest.
- Screening and titering analysis - we screen and characterize the antisera or hybridoma supernatants produced, and we will perform nearly any assay development and validation experiment you request.
- Purification specificities and deliverables - we purify all antibodies with an effective three-step procedure; in addition, we offer many specialized purification options for obtaining monospecific antibodies, such as to phosphorylated, acetylated or other post-translational modification state.
- Online project tracking and management - all antibody production services use our exclusive Thermo Scientific OpenProject Tool, a web portal that gives you real-time information about the status and progress of your antibody production projects, as well as complete management control over next steps.
Details and Additional Information:
Get Started Now!
Is this your first time considering custom antibody production? No problem!
- Study the Highlights (above) and related informational pages (above right).
- Choose "Request a Quote" and our form will guide you through the details.
Summary of Service Capabilities and Options:
The following table summarizes some of our more popular custom antibody production packages, options and corresponding features.
| Polyclonal Antibody Production | Monoclonal Antibody Production | ||||||
|---|---|---|---|---|---|---|---|
| Recombinant Protein Antibody |
Standard Anti-Peptide Antibody |
Modification Specific Antibody† |
Modification Specific Antibody† |
Standard Anti-Peptide Antibody |
Recombinant Protein Antibody |
||
| Antigen Design with Antigen Profiler |
✔ | ✔ | ✔ | ✔ | ✔ | ✔ | Antigen Design with Antigen Profiler |
| Peptide Antigen Synthesis |
NA | ✔ | ✔ | ✔ | ✔ | NA | Peptide Antigen Synthesis |
| Depleting Peptide Synthesis |
NA | NA | ✔ | ✔ | NA | NA | Control Peptide Synthesis |
| Protein Expression (vs. you supply) |
Optional | NA | NA | NA | NA | Optional | Protein Expression (vs. you supply) |
| Animal Immunization (8 protocol options) |
✔ | ✔ | ✔ | ✔ | ✔ | ✔ | Animal Immunization (3 protocol options) |
| Bleeds, Serum Prep, ELISA Screening and Titration |
✔ | ✔ | ✔ | ✔ | ✔ | ✔ | Hybridoma Fusion, Cloning, Screening and ELISA Titration |
| Affinity Purification and Depletion (several options) |
Optional | Optional | ✔ | Optional | Optional | Optional | Culture, Production and Purification (several options) |
| Specific Assay Validation or Antibody Labeling |
Optional | Optional | Optional | Optional | Optional | Optional | Specific Assay Validation or Antibody Labeling |
| Complete Online Project Tracking |
✔ | ✔ | ✔ | Coming soon |
Coming soon |
Coming soon |
Complete Online Project Tracking |
| † Modification specific antibodies are phosphospecific antibodies or monospecific antibodies against peptides with other post-translational modifications or specific states (phosphorylation, sumoylation, myristolation, acetylation, polymorphisms, drug binding, ubiquitination, glycosylation, isoforms, splice variants, ligand binding, prenylation, protein cleavage neo-epitopes, mutations, species cross-reactivity). | |||||||
Our scientists have many years of experience developing over 16,000 custom monoclonal and polyclonal antibodies for academic, government and commercial labs throughout the world. Through our Antibody Production Service, you have access to exclusive resources developed and optimized based on our experience, including proprietary and proven methods of antigen design, peptide conjugation, immunization and purification.
