c-Myc Antibody (9E10)

c-Myc Monoclonal Antibody for Western blot, IF, ICC, IHC (P, F), FACS, IP, ChIP, ELISA

>> See 13 other antibodies for MYC (c Myc, Cellular myelocytomatosis oncogene, Myc protein, Myc proto oncogene protein, Myc2, Niard, Nird, v myc avian myelocytomatosis viral oncogene hom)
Synonyms:
c Myc, Cellular myelocytomatosis oncogene, Myc protein, Myc proto oncogene protein, Myc2, Niard, Nird, v myc avian myelocytomatosis viral oncogene hom
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Mouse / IgG
Class: Monoclonal
Type: Antibody
Clone: 9E10
Tested Species Reactivity: Human (Hu)
Published Species Reactivity: Human (Hu), Not Applicable (N/A), Rat (Rt), Yeast (Ys)
Immunogen: Synthetic peptide A(408) E E Q K L I S E E D L L R K R R E Q L K H K L E Q L R N S C A(438) of human c-Myc.
Ordering Information
Pierce c-Myc Antibody (9E10)
Product #
MA1-980
Size
100 µg
Price
$230.00
Purchase
Add c-Myc Antibody (9E10) to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:500-1:2000
Immunofluorescence (IF) 1:100-1:500
Immunocytochemistry (ICC) 1:100-1:500
Immunohistochemistry (Paraffin, Frozen) (IHC (P, F)) 1:50 - 1:1000
Flow Cytometry (FACS) 1:50-1:200
Immunoprecipitation (IP) Assay dependent
ChIP assay (ChIP) Assay Dependent
ELISA (ELISA) Assay dependent
Published Applications Dilution
Western Blot (WB) See publications below
Immunocytochemistry (ICC) See publications below
Immunoprecipitation (IP) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 1mg/ml
Purification: Protein A
Storage Buffer: PBS with 1mg/ml BSA
Preservative: 0.05% sodium azide
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
MA1-980 detects c-myc protein and c-myc tagged proteins.

MA1-980 has been successfully used in Western blot, immunohistochemistry, immunocytochemistry, immunofluorescence, ELISA, flow cytometry, and immunoprecipitation procedures.

The MA1-980 immunogen corresponds to the synthetic peptide A(408) E E Q K L I S E E D L L R K R R E Q L K H K L E Q L R N S C A(438) of human c-Myc.
General Information
The c-myc oncogene (p62 c-myc) is involved in the control of normal cellular proliferation and differentiation. In addition, deregulated expression of c-Myc induces apoptosis in different cell types, with c-myc requiring p53 for apoptosis in many cell types. This fact indicates heterogeneous mechanisms for c-myc-induced apoptosis.
Product Images
  • c-Myc Antibody (MA1-980) in WB
    MA1-980_c-myc_9E10_WB1.jpg

    Western Blot with anti-c-Myc Monoclonal Antibody [9E10] (MA1-980) (MA1-980_c-myc_9E10_WB1.jpg)

    Western Blot with anti-c-Myc Monoclonal Antibody [9E10] (MA1-980)

    Western blot analysis of c-Myc was performed by loading 40ug of HeLa or NCCIT nuclear fractions (NF) onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. Membranes were probed with c-Myc Monoclonal Antibody, 9E10 (Product # MA1-980) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-IgG HRP secondary antibody at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection was performed using Super Signal West Dura (Product #34075). Nuclear fractions were generated using the NE-PER kit (Product # 78833).
  • c-Myc Antibody (MA1-980) in WB
    MA1-980_c-myc_9E10_WB2.jpg

    Western Blot with anti-c-Myc Monoclonal Antibody [9E10] (MA1-980) (MA1-980_c-myc_9E10_WB2.jpg)

    Western Blot with anti-c-Myc Monoclonal Antibody [9E10] (MA1-980)

    Western blot analysis of c-Myc was performed by loading 20ug of 293T or 293T-c-myc whole cell lysate onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. Membranes were probed with c-Myc Monoclonal Antibody, 9E10 (Product # MA1-980) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-IgG HRP secondary antibody at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed using Super Signal West Dura (Product #34075).
  • c-Myc Antibody (MA1-980) in WB
    MA1-980_c-myc_9E10_WB3.jpg

    Western Blot with anti-c-Myc Monoclonal Antibody [9E10] (MA1-980) (MA1-980_c-myc_9E10_WB3.jpg)

    Western Blot with anti-c-Myc Monoclonal Antibody [9E10] (MA1-980)

    Western blot analysis of c-Myc was performed by loading 25-100 ng recombinant Myc-tagged protein onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. Membranes were probed with c-Myc Monoclonal Antibody, 9E10 (Product # MA1-980) at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-IgG HRP secondary antibody at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection was performed using Super Signal West Dura (Product #34075).
  • c-Myc Antibody (MA1-980) in IF
    c-Myc_Antibody_MA1-980_Immunofluorescence_1.jpg

    Immunofluorescence with anti-c-Myc Monoclonal Antibody [9E10] (MA1-980) (c-Myc_Antibody_MA1-980_Immunofluorescence_1.jpg)

    Immunofluorescence with anti-c-Myc Monoclonal Antibody [9E10] (MA1-980)

    Immunofluorescent analysis of c-Myc (green) in HEL 11.4 induced IPS cells grown for a few days on Matrigel-coated chamber slides. Cells fixed in 4% paraformaldehyde were permeabilized with 0.1% Triton X-100 for 15 minutes at room temperature. Cells were probed with a c-Myc monoclonal antibody (Product # MA1-980) at a dilution of 1:200 overnight at 4ºC, washed with PBST, and incubated with a fluorescein-conjugated secondary antibody at a dilution of 1:100 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI and cells were analyzed by fluorescence microscopy at 20X magnification.
  • c-Myc Antibody (MA1-980) in IF
    c-Myc_Antibody_MA1-980_Immunofluorescence_2.jpg

    Immunofluorescence with anti-c-Myc Monoclonal Antibody [9E10] (MA1-980) (c-Myc_Antibody_MA1-980_Immunofluorescence_2.jpg)

    Immunofluorescence with anti-c-Myc Monoclonal Antibody [9E10] (MA1-980)

    Immunofluorescent analysis of c-Myc (green) in H9 embryonic stem cells grown for a few days on Matrigel-coated chamber slides. Cells fixed in 4% paraformaldehyde were permeabilized with 0.1% Triton X-100 for 15 minutes at room temperature. Cells were probed with a c-Myc monoclonal antibody (Product # MA1-980) at a dilution of 1:200 overnight at 4ºC, washed with PBST, and incubated with a fluorescein-conjugated secondary antibody at a dilution of 1:100 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI and cells were analyzed by fluorescence microscopy at 20X magnification.
  • c-Myc Antibody (MA1-980) in IHC (P)
    MA1-980_Immunohistochemistry_human breast carcinoma.jpg

    Immunohistochemistry (Paraffin) with anti-c-Myc Monoclonal Antibody [9E10] (MA1-980) (MA1-980_Immunohistochemistry_human breast carcinoma.jpg)

    Immunohistochemistry (Paraffin) with anti-c-Myc Monoclonal Antibody [9E10] (MA1-980)

    Immunohistochemistry was performed on cancer biopsies of deparaffinized Human breast carcinoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a Mouse Monoclonal Antibody recognizing c-myc (MA1-980) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  • c-Myc Antibody (MA1-980) in IHC (P)
    MA1-980_Immunohistochemistry_human lung squamous carcinoma.jpg

    Immunohistochemistry (Paraffin) with anti-c-Myc Monoclonal Antibody [9E10] (MA1-980) (MA1-980_Immunohistochemistry_human lung squamous carcinoma.jpg)

    Immunohistochemistry (Paraffin) with anti-c-Myc Monoclonal Antibody [9E10] (MA1-980)

    Immunohistochemistry was performed on cancer biopsies of deparaffinized Human lung squamous carcinoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a Mouse Monoclonal Antibody recognizing c-myc (MA1-980) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  • c-Myc Antibody (MA1-980) in IHC (P)
    MA1-980_Immunohistochemistry_human tonsil tissue.jpg

    Immunohistochemistry (Paraffin) with anti-c-Myc Monoclonal Antibody [9E10] (MA1-980) (MA1-980_Immunohistochemistry_human tonsil tissue.jpg)

    Immunohistochemistry (Paraffin) with anti-c-Myc Monoclonal Antibody [9E10] (MA1-980)

    Immunohistochemistry was performed on normal biopsies of deparaffinized Human tonsil tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:50 with a Mouse Monoclonal Antibody recognizing c-myc (MA1-980) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  • c-Myc Antibody (MA1-980) in FACS
    c-Myc_Antibody_MA1-980_FACS_1.jpg

    Flow Cytometry with anti-c-Myc Monoclonal Antibody [9E10] (MA1-980) (c-Myc_Antibody_MA1-980_FACS_1.jpg)

    Flow Cytometry with anti-c-Myc Monoclonal Antibody [9E10] (MA1-980)

    Flow cytometric analysis of c-Myc (blue histogram) on HEL 11.4 induced IPS cells. To generate single cells suspensions, colonies were treated with TrypLE cell dissociation enzyme for 5 minutes at 37ºC. Cells were incubated with a c-Myc monoclonal antibody (Product # MA1-980) or mouse IgG (green histogram) at a dilution of 1:100 for 1 hour on ice, washed with PBS + 5% fetal calf serum (FACS buffer), and incubated with a fluorescein-conjugated secondary antibody at a dilution of 1:200 for 30 minutes on ice. Cells were washed with cold FACS buffer, resuspended in 500ul of FACS buffer containing 10ul of 4% paraformaldehyde, and analyzed on a flow cytometer.
  • c-Myc Antibody (MA1-980) in FACS
    c-Myc_Antibody_MA1-980_FACS_2.jpg

    Flow Cytometry with anti-c-Myc Monoclonal Antibody [9E10] (MA1-980) (c-Myc_Antibody_MA1-980_FACS_2.jpg)

    Flow Cytometry with anti-c-Myc Monoclonal Antibody [9E10] (MA1-980)

    Flow cytometric analysis of c-Myc (blue histogram) on H9 embryonic stem cells. To generate single cells suspensions, colonies were treated with TrypLE cell dissociation enzyme for 5 minutes at 37ºC. Cells were incubated with a c-Myc monoclonal antibody (Product # MA1-980) or mouse IgG (green histogram) at a dilution of 1:100 for 1 hour on ice, washed with PBS + 5% fetal calf serum (FACS buffer), and incubated with a fluorescein-conjugated secondary antibody at a dilution of 1:200 for 30 minutes on ice. Cells were washed with cold FACS buffer, resuspended in 500ul of FACS buffer containing 10ul of 4% paraformaldehyde, and analyzed on a flow cytometer.
Publications:
Western Blot
  Species / Dilution Summary
  Hu / 1-10 ug/ml
MA1-980 was used in immunoprecipitation and western blot to introduce the method of epitope tagging

Methods Enzymol. 1991;194():508-19.
"Epitope tagging and protein surveillance."
Author(s): Kolodziej PA, Young RA
Number of Citations: 161
(See PubMed article )
  Hu / 0
MA1-980 was used in western blot to study the role of 14-3-3beta in regulating the TSC2 tumor suppressor gene product tuberin

J Biol Chem. 2003 Jan 24;278(4):2089-92.
"14-3-3beta binds to and negatively regulates the tuberous sclerosis complex 2 (TSC2) tumor suppressor gene product, tuberin."
Author(s): Shumway SD, Li Y, Xiong Y
Number of Citations: 13
(See PubMed article )
  Hu / Not Cited
MA1-980 was used in western blot to investigate N-cadherin processing by proteolysis and the formation of N-cadherin-catenin complexes

J Biol Chem. 2003 May 9;278(19):17269-76.
"N-cadherin-catenin complexes form prior to cleavage of the proregion and transport to the plasma membrane."
Author(s): Wahl JK 3rd, Kim YJ, Cullen JM, Johnson KR, Wheelock MJ
Number of Citations: 1
(See PubMed article )
  Hu / 0
MA1-980 was used in immunoprecipitation and western blot to investigate the mechanism of CDT1 ubiquitination induced by DNA damage

Nat Cell Biol. 2004 Oct;6(10):1003-9.
"Targeted ubiquitination of CDT1 by the DDB1-CUL4A-ROC1 ligase in response to DNA damage."
Author(s): Hu J, McCall CM, Ohta T, Xiong Y
Number of Citations: 1
(See PubMed article )
  Hu / 0
MA1-980 was used in immunoprecipitation and western blot to investigate the influence of cyclin D1 on tumor suppressor TSC1 and 2 function

Cancer Res. 2005 Dec 15;65(24):11354-60.
"Negative regulation of TSC1-TSC2 by mammalian D-type cyclins."
Author(s): Zacharek SJ, Xiong Y, Shumway SD
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
MA1-980 was used in western blot to study the association between ubiquitin ligase cullin 7 binds and p53

Oncogene. 2006 Aug 3;25(33):4534-48.
"Cytoplasmic localized ubiquitin ligase cullin 7 binds to p53 and promotes cell growth by antagonizing p53 function."
Author(s): Andrews P, He YJ, Xiong Y
Number of Citations: 16
(See PubMed article )
  Hu / 0
MA1-980 was used in western blot to investigate the relationship between DKC1 and c-MYC

Biochem Biophys Res Commun. 2007 Nov 3;362(4):893-8.
"DKC1 is a direct and conserved transcriptional target of c-MYC."
Author(s): Alawi F, Lee MN
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
MA1-980 was used in western blot to investigate the phosphorylation of Tsc1 and Tsc2

PLoS One. 2009 Jul 17;4(7):e6305.
"Akt phosphorylates both Tsc1 and Tsc2 in Drosophila, but neither phosphorylation is required for normal animal growth."
Author(s): Schleich S, Teleman AA
Number of Citations: 1
(See PubMed article )
  Hu / 0
MA1-980 was used in western blot to study the interaction of Trf3 and Taf3 during early development and hematopoiesis in zebrafish

Dev Dyn. 2009 Oct;238(10):2540-9.
"Selective interaction between Trf3 and Taf3 required for early development and hematopoiesis."
Author(s): Hart DO, Santra MK, Raha T, Green MR
Number of Citations: 6
(See PubMed article )
  Hu / Not Cited
MA1-980 was used in western blot to study the activation of the PRC-dependent stress program by apoptosis and senescence and its role in the response to cellular dysfunction

J Biol Chem. 2013 Mar 22;288(12):8004-15.
"Activation of a PGC-1-related coactivator (PRC)-dependent inflammatory stress program linked to apoptosis and premature senescence."
Author(s): Gleyzer N, Scarpulla RC
Number of Citations: 1
(See PubMed article )
  N/A / 0
MA1-980 was used in immunoprecipitation and western blot to investigate the involvement of DDB1 in the association of DWD proteins with CUL4A

Genes Dev. 2006 Nov 1;20(21):2949-54.
"DDB1 functions as a linker to recruit receptor WD40 proteins to CUL4-ROC1 ubiquitin ligases."
Author(s): He YJ, McCall CM, Hu J, Zeng Y, Xiong Y
Number of Citations: 1
(See PubMed article )
  N/A / Not Cited
MA1-980 was used in western blot to study the role of Aurora A kinase in the mechanism by which CUL3-KLHL18 regulates cell cycle entry

Biol Open. 2012 Feb 15;1(2):82-91.
"The CUL3-KLHL18 ligase regulates mitotic entry and ubiquitylates Aurora-A."
Author(s): Moghe S, Jiang F, Miura Y, Cerny RL, Tsai MY, Furukawa M
Number of Citations: 3
(See PubMed article )
  Rt / 1:1,000
MA1-980 was used in western blot to study the results of adenoviral SERCA1 gene transfer in Sprague-Dawley rat hearts.

Am J Physiol Heart Circ Physiol. 2008 Dec;295(6):H2483-94.
"Limited functional and metabolic improvements in hypertrophic and healthy rat heart overexpressing the skeletal muscle isoform of SERCA1 by adenoviral gene transfer in vivo."
Author(s): O'Donnell JM, Fields A, Xu X, Chowdhury SA, Geenen DL, Bi J
Number of Citations: 1
(See PubMed article )
Immunocytochemistry
  Species / Dilution Summary
  Hu / Not Cited
MA1-980 was used in immunocytochemistry and immunoprecipitation to produce and characterize monoclonal antibodies against HCMV glycoprotein complexes

J Virol. 1986 Nov;60(2):345-52.
"Characterization of monoclonal antibodies reactive to several biochemically distinct human cytomegalovirus glycoprotein complexes."
Author(s): Kari B, Lussenhop N, Goertz R, Wabuke-Bunoti M, Radeke R, Gehrz R
Number of Citations: 21
(See PubMed article )
  Hu / Not Cited
MA1-980 was used in immunocytochemistry to demonstrate that Ire1 plays a role in multiple facets of the ER stress-response

EMBO J. 1998 Oct 1;17(19):5708-17.
"Cloning of mammalian Ire1 reveals diversity in the ER stress responses."
Author(s): Wang XZ, Harding HP, Zhang Y, Jolicoeur EM, Kuroda M, Ron D
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
MA1-980 was used in immunocytochemistry to investigate the role of the melanocortin-4 receptor in obesity.

J Clin Endocrinol Metab. 2003 Dec;88(12):5841-5.
"Deletion of codons 88-92 of the melanocortin-4 receptor gene: a novel deleterious mutation in an obese female."
Author(s): Donohoue PA, Tao YX, Collins M, Yeo GS, O'Rahilly S, Segaloff DL
Number of Citations: 1
(See PubMed article )
  Hu / 1:100
MA1-980 was used in immunocytochemistry to study the role of MC4R mutations in the pathogenesis of binge eating disorder.

J Clin Endocrinol Metab. 2005 Oct;90(10):5632-8.
"Functional analyses of melanocortin-4 receptor mutations identified from patients with binge eating disorder and nonobese or obese subjects."
Author(s): Tao YX, Segaloff DL
Number of Citations: 1
(See PubMed article )
  Ys / Not Cited
MA1-980 was used in immunocytochemistry to study how Rat7p regulates nucleocytoplasmic export of mRNA

J Cell Biol. 1995 May;129(4):939-55.
"A conditional allele of the novel repeat-containing yeast nucleoporin RAT7/NUP159 causes both rapid cessation of mRNA export and reversible clustering of nuclear pore complexes."
Author(s): Gorsch LC, Dockendorff TC, Cole CN
Number of Citations: 85
(See PubMed article )
Immunoprecipitation
  Species / Dilution Summary
  Hu / Not Cited
MA1-980 was used in immunoprecipitation and western blot to introduce the method of epitope tagging

Methods Enzymol. 1991;194():508-19.
"Epitope tagging and protein surveillance."
Author(s): Kolodziej PA, Young RA
Number of Citations: 161
(See PubMed article )
  Hu / Not Cited
MA1-980 was used in immunocytochemistry and immunoprecipitation to produce and characterize monoclonal antibodies against HCMV glycoprotein complexes

J Virol. 1986 Nov;60(2):345-52.
"Characterization of monoclonal antibodies reactive to several biochemically distinct human cytomegalovirus glycoprotein complexes."
Author(s): Kari B, Lussenhop N, Goertz R, Wabuke-Bunoti M, Radeke R, Gehrz R
Number of Citations: 21
(See PubMed article )
  Hu / Not Cited
MA1-980 was used in immunoprecipitation to investigate the interaction between ROC1 and cullin proteins

Mol Cell. 1999 Apr;3(4):535-41.
"ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity."
Author(s): Ohta T, Michel JJ, Schottelius AJ, Xiong Y
Number of Citations: 98
(See PubMed article )
  Hu / 0
MA1-980 was used in immunoprecipitation and western blot to investigate the mechanism of CDT1 ubiquitination induced by DNA damage

Nat Cell Biol. 2004 Oct;6(10):1003-9.
"Targeted ubiquitination of CDT1 by the DDB1-CUL4A-ROC1 ligase in response to DNA damage."
Author(s): Hu J, McCall CM, Ohta T, Xiong Y
Number of Citations: 1
(See PubMed article )
  Hu / 0
MA1-980 was used in immunoprecipitation and western blot to investigate the influence of cyclin D1 on tumor suppressor TSC1 and 2 function

Cancer Res. 2005 Dec 15;65(24):11354-60.
"Negative regulation of TSC1-TSC2 by mammalian D-type cyclins."
Author(s): Zacharek SJ, Xiong Y, Shumway SD
Number of Citations: 1
(See PubMed article )
  Hu / 0
MA1-980 was used in immunoprecipitation to study the role of the WD40 protein FBW5 in promoting the ubiquitinylation of tumor suppresor protein TSC2

Genes Dev. 2008 Apr 1;22(7):866-71.
"WD40 protein FBW5 promotes ubiquitination of tumor suppressor TSC2 by DDB1-CUL4-ROC1 ligase."
Author(s): Hu J, Zacharek S, He YJ, Lee H, Shumway S, Duronio RJ, Xiong Y
Number of Citations: 25
(See PubMed article )
  N/A / 0
MA1-980 was used in immunoprecipitation and western blot to investigate the involvement of DDB1 in the association of DWD proteins with CUL4A

Genes Dev. 2006 Nov 1;20(21):2949-54.
"DDB1 functions as a linker to recruit receptor WD40 proteins to CUL4-ROC1 ubiquitin ligases."
Author(s): He YJ, McCall CM, Hu J, Zeng Y, Xiong Y
Number of Citations: 1
(See PubMed article )
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