c-Mpl / CD110 Antibody (1H2)

c-Mpl / CD110 Monoclonal Antibody for Western blot, FACS

Thrombopoietin receptor, TPO-R, Myeloproliferative leukemia protein
Entrez Gene ID:
UniProt ID:
Host / Isotype: Mouse / IgG2b
Class: Monoclonal
Type: Antibody
Clone: 1H2
Tested Species Reactivity: Human (Hu)
Immunogen: Purified recombinant fragment of human MPL expressed in E. Coli.
Ordering Information
Pierce c-Mpl / CD110 Antibody (1H2)
Product #
100 ug
Tested Applications Dilution *
Western Blot (WB) 1:500 - 1:2000
Flow Cytometry (FACS) 1:200 - 1:400
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 1mg/ml
Purification: purified
Storage Buffer: PBS with 0.5% proprietary stabilizer
Preservative: 0.05% sodium azide
Storage Conditions: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Product Specific Information
MA5-17127 targets MPL in FACS and WB applications and shows reactivity with Human samples.

The MA5-17127 immunogen is purified recombinant fragment of human MPL expressed in E. Coli.

MA5-17127 detects MPL which has a predicted molecular weight of approximately 71.2kDa.
General Information
In 1990 an oncogene, v-mpl, was identified from the murine myeloproliferative leukemia virus that was capable of immortalizing bone marrow hematopoietic cells from different lineages. In 1992 the human homologue, named, c-mpl, was cloned. Sequence data revealed that c-mpl encoded a protein that was homologous with members of the hematopoietic receptor superfamily. Presence of anti-sense oligodeoxynucleotides of c-mpl inhibited megakaryocyte colony formation. The ligand for c-mpl, thrombopoietin, was cloned in 1994. Thrombopoietin was shown to be the major regulator of megakaryocytopoiesis and platelet formation. The protein encoded by the c-mpl gene, CD110, is a 635 amino acid transmembrane domain, with two extracellular cytokine receptor domains and two intracellular cytokine receptor box motifs . TPO-R deficient mice were severely thrombocytopenic, emphasizing the important role of CD110 and thrombopoietin in megakaryocyte and platelet formation. Upon binding of thrombopoietin CD110 is dimerized and the JAK family of non-receptor tyrosine kinases, as well as the STAT family, the MAPK family, the adaptor protein Shc and the receptors themselves become tyrosine phosphorylated.
Product Images
  • c-Mpl / CD110 Antibody (MA5-17127) in WB

    Western Blot with anti-c-Mpl / CD110 Monoclonal Antibody [1H2] (MA5-17127) (CD110-MPL_Antibody_MA5-17127_Westernblot1-1252013.jpg)

    Western Blot with anti-c-Mpl / CD110 Monoclonal Antibody [1H2] (MA5-17127)

    Western blot analysis of CD110/MPL using a CD110/MPL monoclonal antibody (Product # MA5-17127) against a human MPL (AA: 307-362) recombinant protein.
  • c-Mpl / CD110 Antibody (MA5-17127) in FACS

    Flow Cytometry with anti-c-Mpl / CD110 Monoclonal Antibody [1H2] (MA5-17127) (CD110_MPL_Antibody_MA5-17127_FlowCytometry-1252013.jpg)

    Flow Cytometry with anti-c-Mpl / CD110 Monoclonal Antibody [1H2] (MA5-17127)

    Flow cytometric analysis of MOLT4 cells using CD110/MPL monoclonal antibody (Product # MA5-17127) (green) and negative control (red).
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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