beta Catenin Antibody (9F2)

beta Catenin Monoclonal Antibody for Western blot, IF, IP

>> See 16 other antibodies for CTNNB1 (Cadherin associated protein, Catenin beta 1, CATNB, CTNNB)
Synonyms:
Cadherin associated protein, Catenin beta 1, CATNB, CTNNB
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Mouse / IgG1
Class: Monoclonal
Type: Antibody
Clone: 9F2
Tested Species Reactivity: Human (Hu), Mouse (Ms)
Published Species Reactivity: Human (Hu)
Immunogen: 120 kDa MBP/beta catenin fusion protein purified on amylose column.
Ordering Information
Pierce beta Catenin Antibody (9F2)
Product #
MA1-2001
Size
100 µg
Price
$250.00
Purchase
Add beta Catenin Antibody (9F2) to your cart.
Tested Applications Dilution *
Western Blot (WB) Assay dependent
Immunofluorescence (IF) 10 ug/ml
Immunoprecipitation (IP) 1 ug/ml
Published Applications Dilution
Western Blot (WB) See publications below
Immunocytochemistry (ICC) See publications below
Immunoprecipitation (IP) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 1mg/ml
Purification: Protein G
Storage Buffer: PBS with 1mg/ml BSA
Preservative: 0.05% sodium azide
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
MA1-2001 detects beta catenin from human samples.

MA1-2001 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~94 kDa protein representing beta catenin from A431 cell extract. MA1-2001 can also be used in immunoprecipitation and immunofluorescence procedures.

The MA1-2001 immunogen is a 120 kDa MBP/ beta catenin fusion protein purified via an amylose column.
General Information
The Catenins (alpha, beta and gamma) are ubiquitously expressed cytoplasmic proteins, which are associated with E-cadherin. Beta Cadherin can also bind to N-cadherin and co-immunoprecipitates with APC. Cadherin/Catenin complexes are linked to the cytoskeleton via a direct association between alpha Actinin and alpha Catenin.
Product Images
  • beta Catenin Antibody (MA1-2001) in IF
    MA1-2001_IF_Hela.jpg

    Immunofluorescence with anti-beta Catenin Monoclonal Antibody [9F2] (MA1-2001) (MA1-2001_IF_Hela.jpg)

    Immunofluorescence with anti-beta Catenin Monoclonal Antibody [9F2] (MA1-2001)

    Immunofluorescent analysis of Beta-Catenin using Beta-Catenin Monoclonal antibody (9F2) (Product# MA1-2001) shows staining in HeLa cells. Beta-Catenin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Beta-Catenin (Product# MA1-2001) at a dilution of 1:20 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.
  • beta Catenin Antibody (MA1-2001) in IF
    MA1-2001_IF_U251.jpg

    Immunofluorescence with anti-beta Catenin Monoclonal Antibody [9F2] (MA1-2001) (MA1-2001_IF_U251.jpg)

    Immunofluorescence with anti-beta Catenin Monoclonal Antibody [9F2] (MA1-2001)

    Immunofluorescent analysis of Beta-Catenin using Beta-Catenin Monoclonal antibody (9F2) (Product# MA1-2001) shows staining in U251 glioma cells. Beta-Catenin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Beta-Catenin (Product# MA1-2001) at a dilution of 1:20 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.
Publications:
Western Blot
  Species / Dilution Summary
  Hu / Not Cited
MA1-2001 was used in immunocytochemistry and western blot to investigate the interaction of plakoglobin with N-cadherin and alpha catenin

J Biol Chem. 1995 Aug 25;270(34):20201-6.
"Identification of plakoglobin domains required for association with N-cadherin and alpha-catenin."
Author(s): Sacco PA, McGranahan TM, Wheelock MJ, Johnson KR
Number of Citations: 26
(See PubMed article )
  Hu / Not Cited
MA1-2001 was used in immunoprecipitation and western blot to investigate the interaction between alpha-actinin and N-cadherin/catenin complex

J Cell Biol. 1995 Jul;130(1):67-77.
"Interaction of alpha-actinin with the cadherin/catenin cell-cell adhesion complex via alpha-catenin."
Author(s): Knudsen KA, Soler AP, Johnson KR, Wheelock MJ
Number of Citations: 81
(See PubMed article )
Immunocytochemistry
  Species / Dilution Summary
  Hu / Not Cited
MA1-2001 was used in immunocytochemistry and western blot to investigate the interaction of plakoglobin with N-cadherin and alpha catenin

J Biol Chem. 1995 Aug 25;270(34):20201-6.
"Identification of plakoglobin domains required for association with N-cadherin and alpha-catenin."
Author(s): Sacco PA, McGranahan TM, Wheelock MJ, Johnson KR
Number of Citations: 26
(See PubMed article )
Immunoprecipitation
  Species / Dilution Summary
  Hu / Not Cited
MA1-2001 was used in immunoprecipitation and western blot to investigate the interaction between alpha-actinin and N-cadherin/catenin complex

J Cell Biol. 1995 Jul;130(1):67-77.
"Interaction of alpha-actinin with the cadherin/catenin cell-cell adhesion complex via alpha-catenin."
Author(s): Knudsen KA, Soler AP, Johnson KR, Wheelock MJ
Number of Citations: 81
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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