beta Catenin Antibody (15B8)

beta Catenin Monoclonal Antibody for Western blot, IF, IHC, IHC (P), GS

>> See 16 other antibodies for CTNNB1 (Cadherin associated protein, Catenin beta 1, CATNB, CTNNB, beta-catenin, beta catenin, CTNNB1;)
Synonyms:
Cadherin associated protein, Catenin beta 1, CATNB, CTNNB, beta-catenin, beta catenin, CTNNB1;
Entrez Gene ID:
Details
Host / Isotype: Mouse
Class: Monoclonal
Type: Antibody
Clone: 15B8
Tested Species Reactivity: Human (Hu), Mouse (Ms), Rat (Rt), Non-human primate (Nhp)
Immunogen: Recombinant beta Catenin protein.
Ordering Information
Pierce beta Catenin Antibody (15B8)
Product #
MA1-301
Size
100 µg
Price
$250.00
Purchase
Add beta Catenin Antibody (15B8) to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:500 - 1:1000
Immunofluorescence (IF) 1:50 - 1:1000
Immunohistochemistry (IHC) 1:100-1:1000
Immunohistochemistry (Paraffin) (IHC (P)) 1:100 - 1:1000
Gel Shift (GS) Assay Dependent
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 1mg/ml
Purification: Protein A
Storage Buffer: PBS with 1mg/ml BSA
Preservative: 0.05% sodium azide
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
MA1-301 detects beta Catenin from human, mouse, monkey, and rat samples.

MA1-301 has been successfully used in Western blot, gel shift, immunohistochemistry (paraffin), and immunofluorescence applications. By Western blot this antibody detects a ~94 kDa protein representing beta Catenin.
General Information
Beta Catenin is thought to play a role in cell-cell communication and adhesion, and possibly cell morphology. In the nucleus, beta Catenin serves to co activate a family of Lef/Tcf transcription factors that stimulate transcription of target genes including those encoding cyclin D and c-myc that promote cell proliferation. This influence on cell proliferation is the molecular basis for the role of beta Catenin in tumorgenesis, in particular solid tumors of the breast, colon, liver, lung, gastric, prostate, and skin.
Product Images
  • beta Catenin Antibody (MA1-301) in WB
    b-catenin_MA1-301_WB.jpg

    Western Blot with anti-beta Catenin Monoclonal Antibody [15B8] (MA1-301) (b-catenin_MA1-301_WB.jpg)

    Western Blot with anti-beta Catenin Monoclonal Antibody [15B8] (MA1-301)

    Western blot analysis of beta-Catenin was performed by loading 50µg of the indicated whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a beta-Catenin monoclonal antibody (Product # MA1-301) at a dilution of 1:1000 overnight at 4°C on a rocking platform, washed in TBS-0.1%Tween 20, and probed with a goat anti-mouse IgG-HRP secondary antibody (Product # 31430) at a dilution of 1:20,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080).
  • beta Catenin Antibody (MA1-301) in IF
    b-catenin_MA1-301_IF.jpg

    Immunofluorescence with anti-beta Catenin Monoclonal Antibody [15B8] (MA1-301) (b-catenin_MA1-301_IF.jpg)

    Immunofluorescence with anti-beta Catenin Monoclonal Antibody [15B8] (MA1-301)

    Immunofluorescent analysis of beta-Catenin (green) in A431 and HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a ?-Catenin monoclonal antibody (Product # MA1-301), at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.
  • beta Catenin Antibody (MA1-301) in IF
    Catenin-beta_MA1-301_Antibody_Immunofluorescence_A431.jpg

    Immunofluorescence with anti-beta Catenin Monoclonal Antibody [15B8] (MA1-301) (Catenin-beta_MA1-301_Antibody_Immunofluorescence_A431.jpg)

    Immunofluorescence with anti-beta Catenin Monoclonal Antibody [15B8] (MA1-301)

    Immunofluorescent analysis of Catenin beta in A431 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Catenin beta monoclonal antibody (Product # MA1-301) at a dilution of 1:200 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). Catenin beta staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
  • beta Catenin Antibody (MA1-301) in IF
    Catenin-beta_MA1-301_Antibody_Immunofluorescence_Hela.jpg

    Immunofluorescence with anti-beta Catenin Monoclonal Antibody [15B8] (MA1-301) (Catenin-beta_MA1-301_Antibody_Immunofluorescence_Hela.jpg)

    Immunofluorescence with anti-beta Catenin Monoclonal Antibody [15B8] (MA1-301)

    Immunofluorescent analysis of Catenin beta in HeLa cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Catenin beta monoclonal antibody (Product # MA1-301) at a dilution of 1:200 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). Catenin beta staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
  • beta Catenin Antibody (MA1-301) in IF
    Catenin-beta_MA1-301_Antibody_Immunofluorescence_NIH-3T3.jpg

    Immunofluorescence with anti-beta Catenin Monoclonal Antibody [15B8] (MA1-301) (Catenin-beta_MA1-301_Antibody_Immunofluorescence_NIH-3T3.jpg)

    Immunofluorescence with anti-beta Catenin Monoclonal Antibody [15B8] (MA1-301)

    Immunofluorescent analysis of Catenin beta in NIH-3T3 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Catenin beta monoclonal antibody (Product # MA1-301) at a dilution of 1:200 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). Catenin beta staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
  • beta Catenin Antibody (MA1-301) in IHC
    Catenin-beta_MA1-301_Immunohistochemistry_Human-Colon-carcinoma.jpg

    Immunohistochemistry with anti-beta Catenin Monoclonal Antibody [15B8] (MA1-301) (Catenin-beta_MA1-301_Immunohistochemistry_Human-Colon-carcinoma.jpg)

    Immunohistochemistry with anti-beta Catenin Monoclonal Antibody [15B8] (MA1-301)

    Immunohistochemistry was performed on cancer biopsies of deparaffinized Human colon carcinoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and probed with a Catenin beta monoclonal antibody (Product # MA1-301) at a dilution of 1:200 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  • beta Catenin Antibody (MA1-301) in IHC
    Catenin-beta_MA1-301_Immunohistochemistry_Human-Stomach-tissue.jpg

    Immunohistochemistry with anti-beta Catenin Monoclonal Antibody [15B8] (MA1-301) (Catenin-beta_MA1-301_Immunohistochemistry_Human-Stomach-tissue.jpg)

    Immunohistochemistry with anti-beta Catenin Monoclonal Antibody [15B8] (MA1-301)

    Immunohistochemistry was performed on normal biopsies of deparaffinized Human stomach tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature and probed with a Catenin beta monoclonal antibody (Product # MA1-301) at a dilution of 1:200 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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