beta COP Antibody

beta COP Polyclonal Antibody for Western blot, IF, ICC

>> See 5 other antibodies for COPB1 (Coatomer subunit beta, Beta-coat protein, COPB)
Synonyms:
Coatomer subunit beta, Beta-coat protein, COPB
Entrez Gene ID:
Details
Host / Isotype: Rabbit
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu), Mouse (Ms), Rat (Rt)
Published Species Reactivity: Avian (Av), Bovine (Bv), Hamster (Hm), Human (Hu), Mouse (Ms), Non-human primate (Nhp), Rat (Rt), Yeast (Ys)
Immunogen: Synthetic peptide corresponding to residues E(496) A G E L K P E E E I T V G P V Q K(513) of rat beta-COP.
Ordering Information
Pierce beta COP Antibody
Product #
PA1-061
Size
100 µl
Price
$365.00
Purchase
Add beta COP Antibody to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:1,000
Immunofluorescence (IF) 1:2,000
Immunocytochemistry (ICC) Assay dependent
Published Applications Dilution
Western Blot (WB) See publications below
Immunocytochemistry (ICC) See publications below
Immunoprecipitation (IP) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 4.4mg/ml
Purification: Ammonium sulfate precipitation
Storage Buffer: PBS with 1mg/ml BSA
Preservative: 0.05% sodium azide
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
PA1-061 detects Golgi beta-coatomer protein (beta-COP) from mouse, human and rat tissues.

PA1-061 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects an ~110kDa protein which represents beta-COP from rat brain homogenate. Indirect immunofluorescence staining of beta-COP in NIH-3T3 cells with PA1-061 results in beta-COP localization to the Golgi complex and to distinct vesicular structures scattered throughout the cytoplasm. Golgi localization of beta-COP is sensitive to treatment with brefeldin A or ATP depletion.

The PA1-061 immunogen is a synthetic peptide corresponding to residues E(496) A G E L K P E E E I T V G P V Q K(513) of rat beta-COP. This sequence is completely conserved in human beta-COP.
General Information
Coatomer proteins are involved in regulating transport between the endoplasmic reticulum (ER) and the Golgi complex and in intra-Golgi transport. There exist two coatomer-protein mechanisms (COPI and COPII) and although they have mechanistic parallels, they are molecularly distinct. The COPI coat is comprised of seven subunits (alpha-, beta-, beta'-, gamma-, delta-, epsilon-, and zeta-COP) in a complex called coatomer. Assembly of the coatomer (COPI) onto non-clathrin coated vesicles is regulated by ADP-ribosylation factor (ARF). Vesicle formation, budding, fusion, and disassembly is dependent on GDP-GTP exchange, COPI, and ARF. COPI has been shown to facilitate retrograde intracellular transport from the ER to the Golgi complex. By contrast, COPII facilitates anterograde transport between these subcellular organelles. COPII has been shown to be independently and selectively recruited to the ER relative to COPI subunits.
Product Images
  • beta COP Antibody (PA1-061) in WB
    PA1-061_2_07232010.jpg

    Western Blot with anti-beta COP Polyclonal Antibody (PA1-061) (PA1-061_2_07232010.jpg)

    Western Blot with anti-beta COP Polyclonal Antibody (PA1-061)

    Western blot detection of beta-COP from rat brain homogenate using PA1-061.
  • beta COP Antibody (PA1-061) in IF
    Copb1_Antibody_PA1-061_Immunofluorescence(IF).jpg

    Immunofluorescence with anti-beta COP Polyclonal Antibody (PA1-061) (Copb1_Antibody_PA1-061_Immunofluorescence(IF).jpg)

    Immunofluorescence with anti-beta COP Polyclonal Antibody (PA1-061)

    Immunolocalization of beta-COP in NIH-3T3 cells using PA1-061.
Publications:
Western Blot
  Species / Dilution Summary
  Bv / 1:1,000
PA1-061 was used in western blot to investigate the intracellular localization of phosphorylated eNOS.

J Biol Chem. 2002 Feb 8;277(6):4277-84.
"Localization of endothelial nitric-oxide synthase phosphorylated on serine 1179 and nitric oxide in Golgi and plasma membrane defines the existence of two pools of active enzyme."
Author(s): Fulton D, Fontana J, Sowa G, Gratton JP, Lin M, Li KX, Michell B, Kemp BE, Rodman D, Sessa WC
Number of Citations: 1
(See PubMed article )
  Bv / Not Cited
PA1-061 was used in western blot to investigate the role of COPI in the intra-Golgi transport.

J Biol Chem. 2002 Dec 27;277(52):50355-64.
"In vitro transport on cis and trans sides of the Golgi involves two distinct types of coatomer and ADP-ribosylation factor-independent transport intermediates."
Author(s): Pullikuth AK, Weidman PJ
Number of Citations: 1
(See PubMed article )
  Bv / Not Cited
PA1-061 was used in western blot to study the effect of GTP-dependent vesicle trafficking on eNOS endocytosis and on intracellular nitric oxide production.

J Cell Sci. 2003 Sep 1;116(Pt 17):3645-55.
"Inhibition of GTP-dependent vesicle trafficking impairs internalization of plasmalemmal eNOS and cellular nitric oxide production."
Author(s): Chatterjee S, Cao S, Peterson TE, Simari RD, Shah V
Number of Citations: 1
(See PubMed article )
  Hm / Not Cited
PA1-061 was used in western blot to investigate the role of COPI in the intra-Golgi transport.

J Biol Chem. 2002 Dec 27;277(52):50355-64.
"In vitro transport on cis and trans sides of the Golgi involves two distinct types of coatomer and ADP-ribosylation factor-independent transport intermediates."
Author(s): Pullikuth AK, Weidman PJ
Number of Citations: 1
(See PubMed article )
  Hm / 1:1,000
PA1-061 was used in western blot and immunocytochemistry to demonstrate the regulation of GEAR levels by COG and COPI complexes.

Mol Biol Cell. 2004 May;15(5):2423-35.
"The COG and COPI complexes interact to control the abundance of GEARs, a subset of Golgi integral membrane proteins."
Author(s): Oka T, Ungar D, Hughson FM, Krieger M
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
PA1-061 was used in immunocytochemistry and western blot to investigate the role of valosin-containing protein in mediating the function of the ubiquitin-proteasome system.

J Cell Sci. 2004 Jan 15;117(Pt 2):281-92.
"RNA interference of valosin-containing protein (VCP/p97) reveals multiple cellular roles linked to ubiquitin/proteasome-dependent proteolysis."
Author(s): Wójcik C, Yano M, DeMartino GN
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
PA1-061 was used in western blot to investigate the effect of GLUT4 overexpression or deficiency on the expression of other GLUT4-vesicle proteins.

J Biol Chem. 2004 May 14;279(20):21598-605.
"GLUT4 overexpression or deficiency in adipocytes of transgenic mice alters the composition of GLUT4 vesicles and the subcellular localization of GLUT4 and insulin-responsive aminopeptidase."
Author(s): Carvalho E, Schellhorn SE, Zabolotny JM, Martin S, Tozzo E, Peroni OD, Houseknecht KL, Mundt A, James DE, Kahn BB
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
PA1-061 was used in western blot and immunocytochemistry to investigate the role of the Rab1 in Golgi intermediate compartment and endoplasmic reticulum functions.

Mol Biol Cell. 2006 Apr;17(4):1514-26.
"Rab1 defines a novel pathway connecting the pre-Golgi intermediate compartment with the cell periphery."
Author(s): Sannerud R, Marie M, Nizak C, Dale HA, Pernet-Gallay K, Perez F, Goud B, Saraste J
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
PA1-061 was used in immunocytochemistry, western blot and immunoprecipitation to identify a Golgi-localized acyl transferases and study its effect on the endothelial nitric oxide synthase.

J Cell Biol. 2006 Jul 31;174(3):369-77.
"Identification of Golgi-localized acyl transferases that palmitoylate and regulate endothelial nitric oxide synthase."
Author(s): Fernández-Hernando C, Fukata M, Bernatchez PN, Fukata Y, Lin MI, Bredt DS, Sessa WC
Number of Citations: 1
(See PubMed article )
  Hu / 1:2,000
PA1-061 was used in immunocytochemistry and western blot to study the interactions among IQGAP1, N-cadherin, and beta-catenin in motile cells .

J Biol Chem. 2007 Mar 16;282(11):8545-56.
"IQ-domain GTPase-activating protein 1 regulates beta-catenin at membrane ruffles and its role in macropinocytosis of N-cadherin and adenomatous polyposis coli."
Author(s): Sharma M, Henderson BR
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
PA1-061 was used in western blot to identify structural requirements for syntaxin 17 localization

Biol Cell. 2011 Jul;103(7):333-50.
"Syntaxin 17 cycles between the ER and ERGIC and is required to maintain the architecture of ERGIC and Golgi."
Author(s): Muppirala M, Gupta V, Swarup G
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
PA1-061 was used in western blot to investigate the effect of GLUT4 overexpression or deficiency on the expression of other GLUT4-vesicle proteins.

J Biol Chem. 2004 May 14;279(20):21598-605.
"GLUT4 overexpression or deficiency in adipocytes of transgenic mice alters the composition of GLUT4 vesicles and the subcellular localization of GLUT4 and insulin-responsive aminopeptidase."
Author(s): Carvalho E, Schellhorn SE, Zabolotny JM, Martin S, Tozzo E, Peroni OD, Houseknecht KL, Mundt A, James DE, Kahn BB
Number of Citations: 1
(See PubMed article )
  Ms / 1:2,000
PA1-061 was used in immunocytochemistry and western blot to study the interactions among IQGAP1, N-cadherin, and beta-catenin in motile cells .

J Biol Chem. 2007 Mar 16;282(11):8545-56.
"IQ-domain GTPase-activating protein 1 regulates beta-catenin at membrane ruffles and its role in macropinocytosis of N-cadherin and adenomatous polyposis coli."
Author(s): Sharma M, Henderson BR
Number of Citations: 1
(See PubMed article )
  Nhp / Not Cited
PA1-061 was used in immunocytochemistry and western blot to investigate the functional properties of the palmitoylation of NOS2 at Cys-4

J Biol Chem. 2004 Dec 31;279(53):55682-9.
"Palmitoylation of inducible nitric-oxide synthase at Cys-3 is required for proper intracellular traffic and nitric oxide synthesis."
Author(s): Navarro-Lérida I, Corvi MM, Barrientos AA, Gavilanes F, Berthiaume LG, Rodríguez-Crespo I
Number of Citations: 1
(See PubMed article )
  Nhp / Not Cited
PA1-061 was used in immunocytochemistry and western blot to study the localization of nCL-2 calpain and its proteolytic effect on beta COP

J Biol Chem. 2006 Apr 21;281(16):11214-24.
"Stomach-specific calpain, nCL-2, localizes in mucus cells and proteolyzes the beta-subunit of coatomer complex, beta-COP."
Author(s): Hata S, Koyama S, Kawahara H, Doi N, Maeda T, Toyama-Sorimachi N, Abe K, Suzuki K, Sorimachi H
Number of Citations: 1
(See PubMed article )
  Nhp / 1:2,000
PA1-061 was used in immunoprecipitation and western blot to the mechanism for the intracellular trafficking of KA2 kainate receptors .

J Biol Chem. 2006 Jun 2;281(22):15475-84.
"Intracellular trafficking of KA2 kainate receptors mediated by interactions with coatomer protein complex I (COPI) and 14-3-3 chaperone systems."
Author(s): Vivithanaporn P, Yan S, Swanson GT
Number of Citations: 1
(See PubMed article )
  Rt / Not Cited
PA1-061 was used in immunocytochemistry, immunoprecipitation and western blot to investigate the cellular localization of beta COP and its homology to beta-adaptin

Cell. 1991 Feb 8;64(3):649-65.
"Beta-COP, a 110 kd protein associated with non-clathrin-coated vesicles and the Golgi complex, shows homology to beta-adaptin."
Author(s): Duden R, Griffiths G, Frank R, Argos P, Kreis TE
Number of Citations: 143
(See PubMed article )
  Rt / Not Cited
PA1-061 was used in western blot and immunocytochemistry to investigate the VLDL assembly in rat hepatoma McA-RH7777 cells.

J Biol Chem. 2002 Aug 23;277(34):31187-200.
"Intracellular assembly of very low density lipoproteins containing apolipoprotein B100 in rat hepatoma McA-RH7777 cells."
Author(s): Tran K, Thorne-Tjomsland G, DeLong CJ, Cui Z, Shan J, Burton L, Jamieson JC, Yao Z
Number of Citations: 1
(See PubMed article )
  Rt / Not Cited
PA1-061 was used in western blot and immunocytochemistry to investigate the role of the Rab1 in Golgi intermediate compartment and endoplasmic reticulum functions.

Mol Biol Cell. 2006 Apr;17(4):1514-26.
"Rab1 defines a novel pathway connecting the pre-Golgi intermediate compartment with the cell periphery."
Author(s): Sannerud R, Marie M, Nizak C, Dale HA, Pernet-Gallay K, Perez F, Goud B, Saraste J
Number of Citations: 1
(See PubMed article )
  Rt / 1:1000
PA1-061 was used in western blot to investigate the regulation of Sar1 translocation onto endoplasmic reticulum membrane by H89 sensitive kinase

Int J Biochem Cell Biol. 2011 Mar;43(3):423-30.
"H89 sensitive kinase regulates the translocation of Sar1 onto the ER membrane through phosphorylation of ER-coupled ?-tubulin."
Author(s): Nakagawa H, Miyazaki S, Abe T, Umadome H, Tanaka K, Nishimura K, Komori M, Matsuo S
Number of Citations: 0
(See PubMed article )
Immunocytochemistry
  Species / Dilution Summary
  Av / 1:100
PA1-061 was used in immunocytochemistry and immunocytochemistry to characterize the ribbon synapses in hair cells

J Neurosci. 2010 Sep 15;30(37):12387-99.
"Molecular anatomy of the hair cell's ribbon synapse."
Author(s): Uthaiah RC, Hudspeth AJ
Number of Citations: 1
(See PubMed article )
  Hm / 1:10
PA1-061 was used in immunocytochemistry to investigate the protein transportation mediated by COPI-coated vesicles

Cell. 1997 Jul 25;90(2):335-49.
"Bidirectional transport by distinct populations of COPI-coated vesicles."
Author(s): Orci L, Stamnes M, Ravazzola M, Amherdt M, Perrelet A, Söllner TH, Rothman JE
Number of Citations: 82
(See PubMed article )
  Hm / Not Cited
PA1-061 was used in immunocytochemistry to study the Golgi retention motif of rift valley fever virus GN glycoprotein.

J Virol. 2002 Dec;76(23):12200-10.
"Characterization of the Golgi retention motif of Rift Valley fever virus G(N) glycoprotein."
Author(s): Gerrard SR, Nichol ST
Number of Citations: 18
(See PubMed article )
  Hm / Not Cited
PA1-061 was used in immunocytochemistry to study the mechanism for the cation-independent mannose 6-phosphate receptor endocytosis.

Mol Biol Cell. 2004 Feb;15(2):721-33.
"Endocytosed cation-independent mannose 6-phosphate receptor traffics via the endocytic recycling compartment en route to the trans-Golgi network and a subpopulation of late endosomes."
Author(s): Lin SX, Mallet WG, Huang AY, Maxfield FR
Number of Citations: 1
(See PubMed article )
  Hm / 1:200
PA1-061 was used in western blot and immunocytochemistry to demonstrate the regulation of GEAR levels by COG and COPI complexes.

Mol Biol Cell. 2004 May;15(5):2423-35.
"The COG and COPI complexes interact to control the abundance of GEARs, a subset of Golgi integral membrane proteins."
Author(s): Oka T, Ungar D, Hughson FM, Krieger M
Number of Citations: 1
(See PubMed article )
  Hm / Not Cited
PA1-061 was used in immunocytochemistry to investigate the role of cholesterol during endoplasmic reticulum-to-Golgi transport.

Mol Biol Cell. 2006 Apr;17(4):1593-605.
"Cholesterol is required for efficient endoplasmic reticulum-to-Golgi transport of secretory membrane proteins."
Author(s): Ridsdale A, Denis M, Gougeon PY, Ngsee JK, Presley JF, Zha X
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
PA1-061 was used in immunocytochemistry, immunoprecipitation and western blot to investigate the cellular localization of beta COP and its homology to beta-adaptin

Cell. 1991 Feb 8;64(3):649-65.
"Beta-COP, a 110 kd protein associated with non-clathrin-coated vesicles and the Golgi complex, shows homology to beta-adaptin."
Author(s): Duden R, Griffiths G, Frank R, Argos P, Kreis TE
Number of Citations: 143
(See PubMed article )
  Hu / Not Cited
PA1-061 was used in immunocytochemistry to investigate the association between the PDZ domain-containing peripheral Golgi protein and the Q-SNARE protein syntaxin 6.

J Biol Chem. 2001 Aug 3;276(31):29456-65.
"Association of a novel PDZ domain-containing peripheral Golgi protein with the Q-SNARE (Q-soluble N-ethylmaleimide-sensitive fusion protein (NSF) attachment protein receptor) protein syntaxin 6."
Author(s): Charest A, Lane K, McMahon K, Housman DE
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
PA1-061 was used in immunocytochemistry to investigate the role of BIG2 during the membrane association of AP-1.

J Biol Chem. 2002 Mar 15;277(11):9468-73.
"Overexpression of an ADP-ribosylation factor-guanine nucleotide exchange factor, BIG2, uncouples brefeldin A-induced adaptor protein-1 coat dissociation and membrane tubulation."
Author(s): Shinotsuka C, Yoshida Y, Kawamoto K, Takatsu H, Nakayama K
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
PA1-061 was used in immunocytochemistry to investigate the roles of the vaccinia virus F13L protein and phospholipase D during the trafficking of integral membrane proteins from the Golgi apparatus.

J Virol. 2002 Aug;76(15):7777-89.
"Similarities in the induction of post-Golgi vesicles by the vaccinia virus F13L protein and phospholipase D."
Author(s): Husain M, Moss B
Number of Citations: 9
(See PubMed article )
  Hu / Not Cited
PA1-061 was used in immunocytochemistry to investigate the impact of the AGAP1 on the endocytic compartment and actin.

J Biol Chem. 2002 Dec 13;277(50):48965-75.
"AGAP1, an endosome-associated, phosphoinositide-dependent ADP-ribosylation factor GTPase-activating protein that affects actin cytoskeleton."
Author(s): Nie Z, Stanley KT, Stauffer S, Jacques KM, Hirsch DS, Takei J, Randazzo PA
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
PA1-061 was used in immunocytochemistry to study the role of heparan sulfate in amyloid precursor protein processing.

J Cell Biol. 2003 Oct 13;163(1):97-107.
"Heparan sulfate regulates amyloid precursor protein processing by BACE1, the Alzheimer's beta-secretase."
Author(s): Scholefield Z, Yates EA, Wayne G, Amour A, McDowell W, Turnbull JE
Number of Citations: 1
(See PubMed article )
  Hu / 1:200
PA1-061 was used in immunocytochemistry to investigate the capacity of the golgi apparatus for self-assembly.

Mol Biol Cell. 2003 Dec;14(12):5011-8.
"Capacity of the golgi apparatus for biogenesis from the endoplasmic reticulum."
Author(s): Puri S, Linstedt AD
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
PA1-061 was used in immunocytochemistry and western blot to investigate the role of valosin-containing protein in mediating the function of the ubiquitin-proteasome system.

J Cell Sci. 2004 Jan 15;117(Pt 2):281-92.
"RNA interference of valosin-containing protein (VCP/p97) reveals multiple cellular roles linked to ubiquitin/proteasome-dependent proteolysis."
Author(s): Wójcik C, Yano M, DeMartino GN
Number of Citations: 1
(See PubMed article )
  Hu / 1:75
PA1-061 was used in immunocytochemistry to demonstrate the effect of inappropriate V region glycosylation on antibody production from expressed immunoglobulin genes.

J Immunol. 2004 May 1;172(9):5489-94.
"V region carbohydrate and antibody expression."
Author(s): Gala FA, Morrison SL
Number of Citations: 0
(See PubMed article )
  Hu / Not Cited
PA1-061 was used in immunocytochemistry to determine the role of the Golgi apparatus in cholera toxin trafficking by Exo2.

EMBO Rep. 2004 Jun;5(6):596-601.
"Retrograde transport of cholera toxin from the plasma membrane to the endoplasmic reticulum requires the trans-Golgi network but not the Golgi apparatus in Exo2-treated cells."
Author(s): Feng Y, Jadhav AP, Rodighiero C, Fujinaga Y, Kirchhausen T, Lencer WI
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
PA1-061 was used in western blot and immunocytochemistry to investigate the role of the Rab1 in Golgi intermediate compartment and endoplasmic reticulum functions.

Mol Biol Cell. 2006 Apr;17(4):1514-26.
"Rab1 defines a novel pathway connecting the pre-Golgi intermediate compartment with the cell periphery."
Author(s): Sannerud R, Marie M, Nizak C, Dale HA, Pernet-Gallay K, Perez F, Goud B, Saraste J
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
PA1-061 was used in immunocytochemistry to characterize the SMAP gene family and study its function in vesicle trafficking.

Mol Biol Cell. 2006 Jun;17(6):2592-603.
"SMAP2, a novel ARF GTPase-activating protein, interacts with clathrin and clathrin assembly protein and functions on the AP-1-positive early endosome/trans-Golgi network."
Author(s): Natsume W, Tanabe K, Kon S, Yoshida N, Watanabe T, Torii T, Satake M
Number of Citations: 1
(See PubMed article )
  Hu / 1:500
PA1-061 was used in immunocytochemistry, western blot and immunoprecipitation to identify a Golgi-localized acyl transferases and study its effect on the endothelial nitric oxide synthase.

J Cell Biol. 2006 Jul 31;174(3):369-77.
"Identification of Golgi-localized acyl transferases that palmitoylate and regulate endothelial nitric oxide synthase."
Author(s): Fernández-Hernando C, Fukata M, Bernatchez PN, Fukata Y, Lin MI, Bredt DS, Sessa WC
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
PA1-061 was used in immunocytochemistry to study the gap junction formation and degradation in human prostate cancer cells.

Mol Biol Cell. 2006 Dec;17(12):5400-16.
"Androgen-regulated formation and degradation of gap junctions in androgen-responsive human prostate cancer cells."
Author(s): Mitra S, Annamalai L, Chakraborty S, Johnson K, Song XH, Batra SK, Mehta PP
Number of Citations: 1
(See PubMed article )
  Hu / 1:2,000
PA1-061 was used in immunocytochemistry and western blot to study the interactions among IQGAP1, N-cadherin, and beta-catenin in motile cells .

J Biol Chem. 2007 Mar 16;282(11):8545-56.
"IQ-domain GTPase-activating protein 1 regulates beta-catenin at membrane ruffles and its role in macropinocytosis of N-cadherin and adenomatous polyposis coli."
Author(s): Sharma M, Henderson BR
Number of Citations: 1
(See PubMed article )
  Hu / 0
PA1-061 was used in immunocytochemistry to investigate the mechanism for antibacterial autophagy

Autophagy. 2011 Jan;7(1):17-26.
"Antibacterial autophagy occurs at PI(3)P-enriched domains of the endoplasmic reticulum and requires Rab1 GTPase."
Author(s): Huang J, Birmingham CL, Shahnazari S, Shiu J, Zheng YT, Smith AC, Campellone KG, Heo WD, Gruenheid S, Meyer T, Welch MD, Ktistakis NT, Kim PK, Klionsky DJ, Brumell JH
Number of Citations: 2
(See PubMed article )
  Hu / Not Cited
PA1-061 was used in immunocytochemistry to investigate the subcellular distribution of USP33 isoforms

Traffic. 2011 Nov;12(11):1563-74.
"Isoform-specific localization of the deubiquitinase USP33 to the Golgi apparatus."
Author(s): Thorne C, Eccles RL, Coulson JM, Urbé S, Clague MJ
Number of Citations: 0
(See PubMed article )
  Hu / Not Cited
PA1-061 was used in immunocytochemistry to investigate the role of vaccinia virus L2 protein in viral crescent formation

J Virol. 2011 Dec;85(23):12431-41.
"Vaccinia virus L2 protein associates with the endoplasmic reticulum near the growing edge of crescent precursors of immature virions and stabilizes a subset of viral membrane proteins."
Author(s): Maruri-Avidal L, Weisberg AS, Moss B
Number of Citations: 1
(See PubMed article )
  Ms / 1:100
PA1-061 was used in immunocytochemistry to investigate the localization of the toll-like receptor 4 in the Golgi apparatus.

J Exp Med. 2002 Mar 4;195(5):559-70.
"Toll-like receptor 4 resides in the Golgi apparatus and colocalizes with internalized lipopolysaccharide in intestinal epithelial cells."
Author(s): Hornef MW, Frisan T, Vandewalle A, Normark S, Richter-Dahlfors A
Number of Citations: 41
(See PubMed article )
  Ms / Not Cited
PA1-061 was used in immunocytochemistry to investigate the dynamics of the Golgi apparatus during murine oocyte in vitro maturation

Biol Reprod. 2002 May;66(5):1259-66.
"Golgi apparatus dynamics during mouse oocyte in vitro maturation: effect of the membrane trafficking inhibitor brefeldin A."
Author(s): Moreno RD, Schatten G, Ramalho-Santos J
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
PA1-061 was used in immunocytochemistry to study the interaction between vesicle-associated membrane protein-associated protein-A (VAP-A) and the oxysterol-binding protein and its effect on the export from the endoplasmic reticulum.

J Biol Chem. 2002 Aug 16;277(33):29908-18.
"Vesicle-associated membrane protein-associated protein-A (VAP-A) interacts with the oxysterol-binding protein to modify export from the endoplasmic reticulum."
Author(s): Wyles JP, McMaster CR, Ridgway ND
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
PA1-061 was used in immunocytochemistry to investigate the impact of the AGAP1 on the endocytic compartment and actin.

J Biol Chem. 2002 Dec 13;277(50):48965-75.
"AGAP1, an endosome-associated, phosphoinositide-dependent ADP-ribosylation factor GTPase-activating protein that affects actin cytoskeleton."
Author(s): Nie Z, Stanley KT, Stauffer S, Jacques KM, Hirsch DS, Takei J, Randazzo PA
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
PA1-061 was used in immunocytochemistry to investigate the role of small GTPase Sar1 in Brucella pathogenesis.

Proc Natl Acad Sci U S A. 2005 Feb 1;102(5):1673-8.
"Brucella coopts the small GTPase Sar1 for intracellular replication."
Author(s): Celli J, Salcedo SP, Gorvel JP
Number of Citations: 1
(See PubMed article )
  Ms / 1:2,000
PA1-061 was used in immunocytochemistry and western blot to study the interactions among IQGAP1, N-cadherin, and beta-catenin in motile cells .

J Biol Chem. 2007 Mar 16;282(11):8545-56.
"IQ-domain GTPase-activating protein 1 regulates beta-catenin at membrane ruffles and its role in macropinocytosis of N-cadherin and adenomatous polyposis coli."
Author(s): Sharma M, Henderson BR
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
PA1-061 was used in immunocytochemistry to study intracellular cycles of Brucella abortus and Francisella tularensis in murine macrophages

Methods Mol Biol. 2008;431():133-45.
"Intracellular localization of Brucella abortus and Francisella tularensis in primary murine macrophages."
Author(s): N/A
Number of Citations: 2
(See PubMed article )
  Ms / 1:400
PA1-061 was used in immunocytochemistry to study the role of HSF1 in oogenesis

Dev Biol. 2010 Mar 15;339(2):338-53.
"Lack of maternal Heat Shock Factor 1 results in multiple cellular and developmental defects, including mitochondrial damage and altered redox homeostasis, and leads to reduced survival of mammalian oocytes and embryos."
Author(s): Bierkamp C, Luxey M, Metchat A, Audouard C, Dumollard R, Christians E
Number of Citations: 5
(See PubMed article )
  Ms / 1:100
PA1-061 was used in immunocytochemistry and immunocytochemistry to characterize the ribbon synapses in hair cells

J Neurosci. 2010 Sep 15;30(37):12387-99.
"Molecular anatomy of the hair cell's ribbon synapse."
Author(s): Uthaiah RC, Hudspeth AJ
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
PA1-061 was used in immunocytochemistry to investigate the normal proteolytic processing of cellular prion protein

Neurosci Lett. 2011 Feb 25;490(2):150-5.
"Possible involvement of calpain-like activity in normal processing of cellular prion protein."
Author(s): Hachiya N, Komata Y, Harguem S, Nishijima K, Kaneko K
Number of Citations: 3
(See PubMed article )
  Ms / Not Cited
PA1-061 was used in immunocytochemistry to investigate the role of TRPM7 in cell morphology, polarization and movements

Biochem J. 2011 Mar 15;434(3):513-21.
"TRPM7 regulates polarized cell movements."
Author(s): Su LT, Liu W, Chen HC, González-Pagán O, Habas R, Runnels LW
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
PA1-061 was used in immunocytochemistry to characterize the structure and function of the Sec7 domain of an ADP-ribosylation factor

J Biol Chem. 2011 Oct 21;286(42):36898-906.
"Novel C-terminal motif within Sec7 domain of guanine nucleotide exchange factors regulates ADP-ribosylation factor (ARF) binding and activation."
Author(s): Lowery J, Szul T, Seetharaman J, Jian X, Su M, Forouhar F, Xiao R, Acton TB, Montelione GT, Lin H, Wright JW, Lee E, Holloway ZG, Randazzo PA, Tong L, Sztul E
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
PA1-061 was used in immunocytochemistry to investigate the role of intra-Golgi IgM-glycosaminoglycan complexes in the pathogenesis of immune complex deposition disorders

J Immunol. 2011 Sep 15;187(6):3198-207.
"Intra-Golgi formation of IgM-glycosaminoglycan complexes promotes Ig deposition."
Author(s): Khan SN, Cox JV, Nishimoto SK, Chen C, Fritzler MJ, Hendershot LM, Weigert M, Radic M
Number of Citations: 0
(See PubMed article )
  Nhp / 1:200
PA1-061 was used in immunocytochemistry to investigate the retargeting of the mitochondrial protein p32/gC1Qr in cellular compartments.

J Cell Sci. 2001 Jun;114(Pt 11):2115-23.
"Retargeting of the mitochondrial protein p32/gC1Qr to a cytoplasmic compartment and the cell surface."
Author(s): van Leeuwen HC, O'Hare P
Number of Citations: 7
(See PubMed article )
  Nhp / Not Cited
PA1-061 was used in immunocytochemistry to investigate the dynamics of the Golgi apparatus during murine oocyte in vitro maturation

Biol Reprod. 2002 May;66(5):1259-66.
"Golgi apparatus dynamics during mouse oocyte in vitro maturation: effect of the membrane trafficking inhibitor brefeldin A."
Author(s): Moreno RD, Schatten G, Ramalho-Santos J
Number of Citations: 1
(See PubMed article )
  Nhp / Not Cited
PA1-061 was used in immunocytochemistry to investigate the roles of the vaccinia virus F13L protein and phospholipase D during the trafficking of integral membrane proteins from the Golgi apparatus.

J Virol. 2002 Aug;76(15):7777-89.
"Similarities in the induction of post-Golgi vesicles by the vaccinia virus F13L protein and phospholipase D."
Author(s): Husain M, Moss B
Number of Citations: 9
(See PubMed article )
  Nhp / Not Cited
PA1-061 was used in immunocytochemistry to investigate the role of the COPI during the formation of replication complexes in different Picornaviridae.

J Virol. 2002 Nov;76(21):11113-22.
"Differential requirements for COPI coats in formation of replication complexes among three genera of Picornaviridae."
Author(s): Gazina EV, Mackenzie JM, Gorrell RJ, Anderson DA
Number of Citations: 21
(See PubMed article )
  Nhp / Not Cited
PA1-061 was used in immunocytochemistry to investigate the impact of the AGAP1 on the endocytic compartment and actin.

J Biol Chem. 2002 Dec 13;277(50):48965-75.
"AGAP1, an endosome-associated, phosphoinositide-dependent ADP-ribosylation factor GTPase-activating protein that affects actin cytoskeleton."
Author(s): Nie Z, Stanley KT, Stauffer S, Jacques KM, Hirsch DS, Takei J, Randazzo PA
Number of Citations: 1
(See PubMed article )
  Nhp / Not Cited
PA1-061 was used in immunocytochemistry and western blot to investigate the functional properties of the palmitoylation of NOS2 at Cys-4

J Biol Chem. 2004 Dec 31;279(53):55682-9.
"Palmitoylation of inducible nitric-oxide synthase at Cys-3 is required for proper intracellular traffic and nitric oxide synthesis."
Author(s): Navarro-Lérida I, Corvi MM, Barrientos AA, Gavilanes F, Berthiaume LG, Rodríguez-Crespo I
Number of Citations: 1
(See PubMed article )
  Nhp / Not Cited
PA1-061 was used in immunocytochemistry and western blot to study the localization of nCL-2 calpain and its proteolytic effect on beta COP

J Biol Chem. 2006 Apr 21;281(16):11214-24.
"Stomach-specific calpain, nCL-2, localizes in mucus cells and proteolyzes the beta-subunit of coatomer complex, beta-COP."
Author(s): Hata S, Koyama S, Kawahara H, Doi N, Maeda T, Toyama-Sorimachi N, Abe K, Suzuki K, Sorimachi H
Number of Citations: 1
(See PubMed article )
  Nhp / 1:500
PA1-061 was used in immunocytochemistry to investigate the effect of resveratrol on cAMP accumulation induced by cholera toxin

Toxicon. 2010 Aug 1;56(1):29-35.
"Resveratrol, a natural polyphenolic compound, inhibits cholera toxin-induced cyclic AMP accumulation in Vero cells."
Author(s): Morinaga N, Yahiro K, Noda M
Number of Citations: 1
(See PubMed article )
  Rt / 1:10
PA1-061 was used in immunocytochemistry to investigate the protein transportation mediated by COPI-coated vesicles

Cell. 1997 Jul 25;90(2):335-49.
"Bidirectional transport by distinct populations of COPI-coated vesicles."
Author(s): Orci L, Stamnes M, Ravazzola M, Amherdt M, Perrelet A, Söllner TH, Rothman JE
Number of Citations: 82
(See PubMed article )
  Rt / 1:100
PA1-061 was used in western blot and immunocytochemistry to investigate the VLDL assembly in rat hepatoma McA-RH7777 cells.

J Biol Chem. 2002 Aug 23;277(34):31187-200.
"Intracellular assembly of very low density lipoproteins containing apolipoprotein B100 in rat hepatoma McA-RH7777 cells."
Author(s): Tran K, Thorne-Tjomsland G, DeLong CJ, Cui Z, Shan J, Burton L, Jamieson JC, Yao Z
Number of Citations: 1
(See PubMed article )
  Rt / 1:200
PA1-061 was used in immunocytochemistry to investigate the capacity of the golgi apparatus for self-assembly.

Mol Biol Cell. 2003 Dec;14(12):5011-8.
"Capacity of the golgi apparatus for biogenesis from the endoplasmic reticulum."
Author(s): Puri S, Linstedt AD
Number of Citations: 1
(See PubMed article )
  Rt / Not Cited
PA1-061 was used in immunocytochemistry to investigate the role of Nm23H2 in mammalian ER export.

Mol Biol Cell. 2005 Feb;16(2):835-48.
"Nm23H2 facilitates coat protein complex II assembly and endoplasmic reticulum export in mammalian cells."
Author(s): Kapetanovich L, Baughman C, Lee TH
Number of Citations: 1
(See PubMed article )
  Rt / 1:100
PA1-061 was used in immunocytochemistry to characterize a new transmembrane bZip transcription factor CREB4.

Mol Biol Cell. 2006 Jan;17(1):413-26.
"CREB4, a transmembrane bZip transcription factor and potential new substrate for regulation and cleavage by S1P."
Author(s): Stirling J, O'hare P
Number of Citations: 1
(See PubMed article )
  Rt / Not Cited
PA1-061 was used in western blot and immunocytochemistry to investigate the role of the Rab1 in Golgi intermediate compartment and endoplasmic reticulum functions.

Mol Biol Cell. 2006 Apr;17(4):1514-26.
"Rab1 defines a novel pathway connecting the pre-Golgi intermediate compartment with the cell periphery."
Author(s): Sannerud R, Marie M, Nizak C, Dale HA, Pernet-Gallay K, Perez F, Goud B, Saraste J
Number of Citations: 1
(See PubMed article )
  Rt / 0.22 ug/ml
PA1-061 was used in immunocytochemistry to investigate the mechanism of urocortin endocytosis in cerebral microvessel endothelial cells

J Mol Neurosci. 2007;31(2):171-81.
"Urocortin trafficking in cerebral microvessel endothelial cells."
Author(s): Tu H, Kastin AJ, Bjorbaek C, Pan W
Number of Citations: 4
(See PubMed article )
  Ys / Not Cited
PA1-061 was used in immunocytochemistry to investigate the cellular mechanism of protein trafficking

Science. 1996 Mar 15;271(5255):1526-33.
"Coat proteins and vesicle budding."
Author(s): Schekman R, Orci L
Number of Citations: 207
(See PubMed article )
Immunoprecipitation
  Species / Dilution Summary
  Hu / Not Cited
PA1-061 was used in immunocytochemistry, western blot and immunoprecipitation to identify a Golgi-localized acyl transferases and study its effect on the endothelial nitric oxide synthase.

J Cell Biol. 2006 Jul 31;174(3):369-77.
"Identification of Golgi-localized acyl transferases that palmitoylate and regulate endothelial nitric oxide synthase."
Author(s): Fernández-Hernando C, Fukata M, Bernatchez PN, Fukata Y, Lin MI, Bredt DS, Sessa WC
Number of Citations: 1
(See PubMed article )
  Nhp / Not Cited
PA1-061 was used in immunoprecipitation to study the formation and dissolution of coatomer

J Biol Chem. 1995 Dec 29;270(52):31364-71.
"In vitro assembly and disassembly of coatomer."
Author(s): Lowe M, Kreis TE
Number of Citations: 22
(See PubMed article )
  Nhp / Not Cited
PA1-061 was used in immunoprecipitation and western blot to the mechanism for the intracellular trafficking of KA2 kainate receptors .

J Biol Chem. 2006 Jun 2;281(22):15475-84.
"Intracellular trafficking of KA2 kainate receptors mediated by interactions with coatomer protein complex I (COPI) and 14-3-3 chaperone systems."
Author(s): Vivithanaporn P, Yan S, Swanson GT
Number of Citations: 1
(See PubMed article )
  Rt / Not Cited
PA1-061 was used in immunocytochemistry, immunoprecipitation and western blot to investigate the cellular localization of beta COP and its homology to beta-adaptin

Cell. 1991 Feb 8;64(3):649-65.
"Beta-COP, a 110 kd protein associated with non-clathrin-coated vesicles and the Golgi complex, shows homology to beta-adaptin."
Author(s): Duden R, Griffiths G, Frank R, Argos P, Kreis TE
Number of Citations: 143
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific