alpha ENaC Antibody

alpha ENaC Polyclonal Antibody for Western blot

>> See 3 other antibodies for SCNN1A (alpha-epithelial sodium channel)
Synonyms:
alpha-epithelial sodium channel
Entrez Gene ID:
Details
Host / Isotype: Rabbit / IgG
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu), Mouse (Ms), Rat (Rt)
Published Species Reactivity: Human (Hu), Mouse (Ms), Rat (Rt)
Immunogen: Synthetic peptide corresponding to residues L(20) M K G N K R E E Q G L G P E P A A P Q Q P T(42) C of Human alpha-Enac.
Ordering Information
Pierce alpha ENaC Antibody
Product #
PA1-920A
Size
100 µg
Price
$365.00
Purchase
Add alpha ENaC Antibody to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:100-1:1000
Published Applications Dilution
Western Blot (WB) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 1mg/ml
Purification: Antigen affinity chromatography
Storage Buffer: PBS with 1mg/ml BSA
Preservative: 0.05% sodium azide
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
PA1-920A detects alpha-epithelial sodium channel (alpha-ENaC) from human, mouse, and rat tissues and cells.

PA1-920A has been successfully used in Western blot procedures. By Western blot, this antibody specifically detects a ~97 kDa band representing glycosylated alpha-ENaC from NIH-3T3 cells, and a ~75 kDa band representing the unglycosylated alpha-ENaC protein from human brain samples.

The PA1-920A immunizing peptide is a synthetic peptide corresponding to residues 20-42 from human alpha-ENaC. This sequence is 82%, 73%, 64%, and 59% conserved in the rat and rabbit, mouse, guinea pig, and cow, respectively. PA1-920A immunizing peptide (Cat. # PEP-088) is available for use in neutralization and control experiments.
General Information
Epithelial sodium channels are amiloride-sensitive members of the degenerin/epithelial sodium channel (Deg/ENaC) superfamily of ion channels. Members of this superfamily of ion channels share organizational similarity in that they all possess two short intracellular amino and carboxyl termini, two short membrane spanning segments, and a large extracellular loop with a conserved cysteine-rich region. There are three homologous isoforms of the ENaC (alpha, beta, and gamma) protein. ENaC in the kidney, lung, and colon plays an essential role in trans-epithelial sodium and fluid balance. ENaC also mediates aldosterone-dependent sodium reabsorption in the distal nephron of the kidney, thus regulating blood pressure. ENaC is thought to be regulated, in part, through association with the cystic fibrosis transmembrane conductance regulator (CFTR) chloride ion channel. Gain-of-function mutations in beta- or gamma-ENaC can cause severe arterial hypertension (Liddel’s syndrome) and loss-of-function mutations in alpha- or beta-ENaC causes pseudohypoaldosteronism (PHA-1).
Product Images
  • alpha ENaC Antibody (PA1-920A) in WB
    PA1-920A_WB_20131010131402.jpg

    Western Blot with anti-alpha ENaC Polyclonal Antibody (PA1-920A) (PA1-920A_WB_20131010131402.jpg)

    Western Blot with anti-alpha ENaC Polyclonal Antibody (PA1-920A)

    Western blot analysis of alpha ENaC was performed by loading 25 ug of Daudi (lane 1) and mouse brain (lane 2) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a alpha ENaC polyclonal antibody (Product # PA1-920A) at a dilution of 1:500 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~80kDa.
Publications:
Western Blot
  Species / Dilution Summary
  Hu / Not Cited
PA1-920A was used in western blot to investigate the role of CFTR in the modulation of epithelial sodium channel expression and function

Am J Physiol Lung Cell Mol Physiol. 2011 Jan;300(1):L88-L101.
"Regulation of endogenous ENaC functional expression by CFTR and ?F508-CFTR in airway epithelial cells."
Author(s): Rubenstein RC, Lockwood SR, Lide E, Bauer R, Suaud L, Grumbach Y
Number of Citations: 2
(See PubMed article )
  Ms / 1:500
PA1-920A was used in western blot to investigate the effect of interleukin-6 on epithelial sodium channel function in mouse cortical collecting duct cells

Am J Physiol Regul Integr Comp Physiol. 2010 Aug;299(2):R590-5.
"Interleukin-6 stimulates epithelial sodium channels in mouse cortical collecting duct cells."
Author(s): Li K, Guo D, Zhu H, Hering-Smith KS, Hamm LL, Ouyang J, Dong Y
Number of Citations: 2
(See PubMed article )
  Ms / Not Cited
PA1-920A was used in western blot to study the inverse relationship between alveolar epithelial sodium channel activity and cystic fibrosis transmembrane conductance regulator expression in mice

Am J Physiol Lung Cell Mol Physiol. 2011 Oct;301(4):L557-67.
"Enhancement of alveolar epithelial sodium channel activity with decreased cystic fibrosis transmembrane conductance regulator expression in mouse lung."
Author(s): Lazrak A, Jurkuvenaite A, Chen L, Keeling KM, Collawn JF, Bedwell DM, Matalon S
Number of Citations: 0
(See PubMed article )
  Rt / 2ug/ml
PEP-088 was used in western blot to study the interaction of amiloride-sensitive epithelial sodium channel and F-actin

J Biol Chem. 2006 Mar 10;281(10):6528-38.
"The carboxyl terminus of the alpha-subunit of the amiloride-sensitive epithelial sodium channel binds to F-actin."
Author(s): Mazzochi C, Bubien JK, Smith PR, Benos DJ
Number of Citations: 1
(See PubMed article )
  Rt / 1:1000
PA1-920A was used in western blot to determine the effect of long-term terbutaline treatment on steady-state expression levels of ENaC in rat fetal distal lung epithelial cells

Am J Physiol Lung Cell Mol Physiol. 2010 Jan;298(1):L96-L104.
"Long-term terbutaline exposure stimulates alpha1-Na+-K+-ATPase expression at posttranscriptional level in rat fetal distal lung epithelial cells."
Author(s): Rahman MS, Gandhi S, Otulakowski G, Duan W, Sarangapani A, O'Brodovich H
Number of Citations: 0
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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