Part of Thermo Fisher Scientific

alpha Adaptin Monoclonal Antibody (AC1-M11)

alpha Adaptin Antibody for IF, IP, Western blot

Synonyms:
AP2
Entrez Gene ID:
(Human) 160, (Murine) 11771
UniProt ID:
O95782
Details
Host / Isotype: Mouse / IgG2a
Class: Monoclonal
Type: Antibody
Clone: AC1-M11
Species Reactivity: Human (Hu) Murine (Ms) Rat (Rt) Non-human primate (Nhp) Bovine (Bv) Porcine (Po) Chicken (Ck) Amphibian (Am)
Immunogen: Purified bovine brain adaptor complexes.
Ordering Information
Pierce alpha Adaptin Monoclonal Antibody (AC1-M11)
Product Number Pkg. Size Price Purchase
MA3-061 500 µl $357.00
Add alpha Adaptin Monoclonal Antibody (AC1-M11) to your cart.


Storage: -20º C, Avoid Freeze/Thaw Cycles
Form: 500 µl of Protein A purified IgG in PBS containing 0.05% sodium azide.


Applications Dilution *
Western Blot (WB) 1:100
Immunofluorescence (IF) 1:100
Immunoprecipitation (IP) Assay dependent
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
MA3-061 detects alpha-adaptin from bovine, chicken, frog, human, monkey, mouse, pig and rat. This antibody detects the products of both alpha-adaptin genes, alphaA and alphaC, as well as an alternatively spliced isoform of alphaA found in neurons.

MA3-061 has been successfully used in Western blot, immunofluorescence, and immunoprecipitation procedures. By Western blot, this antibody recognizes an ~100 kDa doublet representing the two isoforms of alpha-adaptin from pig brain extract. Immunofluorescence staining of alpha-adaptin in transfected COS cells with MA3-061A yields distinct punctate dots on the plasma membrane.

The MA3-061 antigen is purified bovine brain adaptor complexes.

Figure 1 shows immunolocalization of alpha-adaptin in NRK cells using MA3-061.
General Information
Clathrin-mediated endocytosis is the pathway by which many receptors for nutrients and hormones are internalized to be recycled or down-regulated. During formation of clathrin coated membranes, clathrin co-assembles with heterotetrameric molecules known as assembly polypeptides (APs) or adaptors which form a layer of protein between the clathrin lattice and the membrane.

There are two characterized adaptors AP1 and AP2. AP1 is associated with clathrin coated vesicles at the trans-Golgi network and AP2 is associated with the endocytic clathrin coated vesicles at the plasma membrane and has been shown to specifically interact with Src homologous and collagen-like (Shc) and epidermal growth factor (EGF) receptor. AP2 is composed of four subunits, two separate 100 kDa gene products with similar domain structures (alpha and beta adaptin) and a 50 and 17 kDa subunit. There are two alpha-adaptin genes, alphaA and alphaC which have a tissue specific pattern of expression.
Product Images
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  • IF using MA3-061
    Immunofluorescence with anti-alpha Adaptin Monoclonal Antibody [AC1-M11] (MA3-061)

    Immunofluorescence with anti-alpha Adaptin Monoclonal Antibody [AC1-M11] (MA3-061)

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PubMed References:
ELISA
  Species / Dilution Summary
  Bv / 5 ug/ml
 MA3-061 was used in ELISA and immunocytochemistry to reconstitute the process of initiation of receptor-mediated endocytosis by nucleation of clathrin-coated pits in vitro and study factors affecting membrane binding of intact AP2 adaptor molecules
EMBO J. 1993 May;12(5):2169-80.
"Adaptor self-aggregation, adaptor-receptor recognition and binding of alpha-adaptin subunits to the plasma membrane contribute to recruitment of adaptor (AP2) components of clathrin-coated pits."
Author(s): Chang MP, Mallet WG, Mostov KE, Brodsky FM
Number of Citations: 17
(See PubMed article )
Immunocytochemistry
  Species / Dilution Summary
  Bv / 5 ug/ml
 MA3-061 was used in ELISA and immunocytochemistry to reconstitute the process of initiation of receptor-mediated endocytosis by nucleation of clathrin-coated pits in vitro and study factors affecting membrane binding of intact AP2 adaptor molecules
EMBO J. 1993 May;12(5):2169-80.
"Adaptor self-aggregation, adaptor-receptor recognition and binding of alpha-adaptin subunits to the plasma membrane contribute to recruitment of adaptor (AP2) components of clathrin-coated pits."
Author(s): Chang MP, Mallet WG, Mostov KE, Brodsky FM
Number of Citations: 17
(See PubMed article )
  Hu / Not Cited
 MA3-061 was used in immunocytochemistry to investigate the mechanism of endocytosis of adherens junction proteins and tight junction proteins in T84 epithelial cells
Mol Biol Cell. 2004 Jan;15(1):176-88.
"Endocytosis of epithelial apical junctional proteins by a clathrin-mediated pathway into a unique storage compartment."
Author(s): Ivanov AI, Nusrat A, Parkos CA
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
 MA3-061 was used in immunocytochemistry to study the mechanism for the translocation of Stat3 through the cytoplasm to the nucleus
EMBO J. 2002 Jul 1;21(13):3255-63.
"Cytoplasmic transport of Stat3 by receptor-mediated endocytosis."
Author(s): Bild AH, Turkson J, Jove R
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
 MA3-061 was used in immunocytochemistry to investigate the role of dynamin 1 in nervous system function
Science. 2007 Apr 27;316(5824):570-4.
"A selective activity-dependent requirement for dynamin 1 in synaptic vesicle endocytosis."
Author(s): Ferguson SM, Brasnjo G, Hayashi M, Wölfel M, Collesi C, Giovedi S, Raimondi A, Gong LW, Ariel P, Paradise S, O'toole E, Flavell R, Cremona O, Miesenböck G, Ryan TA, De Camilli P
Number of Citations: 1
(See PubMed article )
Immunohistochemistry
  Species / Dilution Summary
  Hu / Not Cited
 MA3-061 was used in immunohistochemistry to investigate the interaction of SPIN90 and syndapin and its role in clathrin-mediated endocytic pathway
Genes Cells. 2006 Oct;11(10):1197-211.
"Interaction of SPIN90 with syndapin is implicated in clathrin-mediated endocytic pathway in fibroblasts."
Author(s): Kim SH, Choi HJ, Lee KW, Hong NH, Sung BH, Choi KY, Kim SM, Chang S, Eom SH, Song WK
Number of Citations: 1
(See PubMed article )
Immunoprecipitation
  Species / Dilution Summary
  Ms / 1:100
 MA3-061 was used in immunoprecipitation and western blot to investigate the role of alpha-adaptin during endocytosis.
J Cell Biol. 2001 Sep 17;154(6):1209-23.
"The actin-binding protein Hip1R associates with clathrin during early stages of endocytosis and promotes clathrin assembly in vitro."
Author(s): Engqvist-Goldstein AE, Warren RA, Kessels MM, Keen JH, Heuser J, Drubin DG
Number of Citations: 1
(See PubMed article )
Western Blot
  Species / Dilution Summary
  Am / 1:100
 MA3-061 was used in western blot to demonstrate the function of xARH in the interactions among LDL, vitellogenin receptors, and the AP-2 complex.
J Biol Chem. 2003 Nov 7;278(45):44584-92.
"Xenopus autosomal recessive hypercholesterolemia protein couples lipoprotein receptors with the AP-2 complex in oocytes and embryos and is required for vitellogenesis."
Author(s): Zhou Y, Zhang J, King ML
Number of Citations: 1
(See PubMed article )
  Bv / 1:100
 MA3-061 was used in western blot to study coat protein membrane binding properties in the Golgi region
J Cell Biol. 1992 Jun;117(6):1171-9.
"100-kD proteins of Golgi- and trans-Golgi network-associated coated vesicles have related but distinct membrane binding properties."
Author(s): Wong DH, Brodsky FM
Number of Citations: 36
(See PubMed article )
  Hu / Not Cited
 MA3-061 was used in western blot to investigate the subcellular localization of CALM protein and its role on clathrin-mediated traffic
Mol Biol Cell. 1999 Aug;10(8):2687-702.
"Clathrin assembly lymphoid myeloid leukemia (CALM) protein: localization in endocytic-coated pits, interactions with clathrin, and the impact of overexpression on clathrin-mediated traffic."
Author(s): Tebar F, Bohlander SK, Sorkin A
Number of Citations: 22
(See PubMed article )
  Ms / 1:1,000
 MA3-061 was used in immunoprecipitation and western blot to investigate the role of alpha-adaptin during endocytosis.
J Cell Biol. 2001 Sep 17;154(6):1209-23.
"The actin-binding protein Hip1R associates with clathrin during early stages of endocytosis and promotes clathrin assembly in vitro."
Author(s): Engqvist-Goldstein AE, Warren RA, Kessels MM, Keen JH, Heuser J, Drubin DG
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
 MA3-061 was used in western blot to investigate the effects of siRNA-mediated silencing of fas-associated death domain (FADD) on septic lung inflammation and apoptosis
Am J Respir Crit Care Med. 2009 May 1;179(9):806-15.
"Silencing of fas-associated death domain protects mice from septic lung inflammation and apoptosis."
Author(s): Matsuda N, Yamamoto S, Takano K, Kageyama S, Kurobe Y, Yoshihara Y, Takano Y, Hattori Y
Number of Citations: 1
(See PubMed article )
  Nhp / Not Cited
 MA3-061 was used in western blot to investigate the subcellular localization of CALM protein and its role on clathrin-mediated traffic
Mol Biol Cell. 1999 Aug;10(8):2687-702.
"Clathrin assembly lymphoid myeloid leukemia (CALM) protein: localization in endocytic-coated pits, interactions with clathrin, and the impact of overexpression on clathrin-mediated traffic."
Author(s): Tebar F, Bohlander SK, Sorkin A
Number of Citations: 22
(See PubMed article )
  Nhp / 1:100
 MA3-061 was used in western blot to study the mechanism for the internalization of the growth hormone receptor.
Mol Endocrinol. 1999 Nov;13(11):1823-31.
"A beta-turn endocytic code is required for optimal internalization of the growth hormone receptor but not for alpha-adaptin association."
Author(s): Vleurick L, Pezet A, Kühn ER, Decuypere E, Edery M
Number of Citations: 1
(See PubMed article )
  Rt / Not Cited
 MA3-061 was used in western blot to investigate the colocalization of VGLUT and VGAT and its effect on neurotransmitter uptake
J Neurosci. 2010 Jun 2;30(22):7634-45.
"Synaptic and vesicular coexistence of VGLUT and VGAT in selected excitatory and inhibitory synapses."
Author(s): Zander JF, Münster-Wandowski A, Brunk I, Pahner I, Gómez-Lira G, Heinemann U, Gutiérrez R, Laube G, Ahnert-Hilger G
Number of Citations: 1
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)