XRCC1 Antibody, Biotin conjugate (33-2-5)

XRCC1 Monoclonal Antibody for Western blot, IHC (P), IP

>> See 5 other antibodies for XRCC1 (DNA repair protein XRCC1, X-ray repair cross-complementing protein 1, X-ray-repair, complementing defective, repair in Chinese hamster)
Synonyms:
DNA repair protein XRCC1, X-ray repair cross-complementing protein 1, X-ray-repair, complementing defective, repair in Chinese hamster
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Mouse / IgG2b
Class: Monoclonal
Type: Antibody
Clone: 33-2-5
Label: Biotin
Tested Species Reactivity: Human (Hu), Rat (Rt)
Published Species Reactivity: Human (Hu)
Immunogen: Recombinant human XRCC1 protein
Ordering Information
Pierce XRCC1 Antibody, Biotin conjugate (33-2-5)
Product #
MA5-13409
Size
500 ul
Price
$425.00
Purchase
Add XRCC1 Antibody, Biotin conjugate (33-2-5) to your cart.
Tested Applications Dilution *
Western Blot (WB) 1-2 µg/ml
Immunohistochemistry (Paraffin) (IHC (P)) 1-2 µg/ml
Immunoprecipitation (IP) 2 µg/ml
Published Applications Dilution
Western Blot (WB) See publications below
Immunocytochemistry (ICC) See publications below
Immunohistochemistry (IHC) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 0.2mg/ml
Purification: Protein A
Storage Buffer: PBS, pH 7.4, with 0.2% BSA
Preservative: 0.09% sodium azide
Storage Conditions: 4° C, do not freeze
Product Specific Information
MA5-13409 targets XRCC1 in IHC (P), IP, and WB applications and shows reactivity with Human and Rat samples.

The MA5-13409 immunogen is recombinant human XRCC1 protein.
General Information
X-Ray Repair Cross Complementing (XRCC1) plays a role in excision repair of DNA after ionizing irradiation. XRCC1 binds to DNA ligase III through the C-terminal 96 amino acids and to DNA polymerase beta through the N-terminal half. In testis XRCC1 is expressed at high levels. Cells with a mutation of this gene exhibit decreased single strand break repair and reduced recombination repair. They show increased double strand breaks, and sister chromatid exchange is increased up to 10-fold. The XRCC1 might serve as a scaffold protein during base excision-repair.
Product Images
  • XRCC1 Antibody (MA5-13409) in WB
    MA5-13409_WesternBlot.jpg

    Western Blot with anti-XRCC1 Monoclonal Antibody [33-2-5], Biotin conjugate (MA5-13409) (MA5-13409_WesternBlot.jpg)

    Western Blot with anti-XRCC1 Monoclonal Antibody [33-2-5], Biotin conjugate (MA5-13409)

    Western blot of XRCC1 using XRCC1 Monoclonal Antibody (MA5-13409) on LS174T Cells.
  • XRCC1 Antibody (MA5-13409) in IHC
    MA5-13409_Immunohistochemistry.jpg

    Immunohistochemistry with anti-XRCC1 Monoclonal Antibody [33-2-5], Biotin conjugate (MA5-13409) (MA5-13409_Immunohistochemistry.jpg)

    Immunohistochemistry with anti-XRCC1 Monoclonal Antibody [33-2-5], Biotin conjugate (MA5-13409)

    Formalin-fixed, paraffin-embedded human testis stained with XRCC1 antibody using peroxidase-conjugate and AEC chromogen. Note nuclear staining of spermatocytes.
  • XRCC1 Antibody (MA5-13409) in IHC
    MA5-13409_Immunohistochemistry2.jpg

    Immunohistochemistry with anti-XRCC1 Monoclonal Antibody [33-2-5], Biotin conjugate (MA5-13409) (MA5-13409_Immunohistochemistry2.jpg)

    Immunohistochemistry with anti-XRCC1 Monoclonal Antibody [33-2-5], Biotin conjugate (MA5-13409)

    Formalin-fixed, paraffin-embedded rat testis stained with XRCC1 antibody using peroxidase-conjugate and AEC chromogen. Note nuclear staining of spermatocytes.
  • XRCC1 Antibody (MA5-13409) in IHC
    MA5-13409_Immunohistochemistry_2.jpg

    Immunohistochemistry with anti-XRCC1 Monoclonal Antibody [33-2-5], Biotin conjugate (MA5-13409) (MA5-13409_Immunohistochemistry_2.jpg)

    Immunohistochemistry with anti-XRCC1 Monoclonal Antibody [33-2-5], Biotin conjugate (MA5-13409)

  • XRCC1 Antibody (MA5-13409) in IP
    MA5-13409_Immunoprecipitation.jpg

    Immunoprecipitation with anti-XRCC1 Monoclonal Antibody [33-2-5], Biotin conjugate (MA5-13409) (MA5-13409_Immunoprecipitation.jpg)

    Immunoprecipitation with anti-XRCC1 Monoclonal Antibody [33-2-5], Biotin conjugate (MA5-13409)

    Immunoprecipitation of XRCC1 using XRCC1 Monoclonal Antibody (MA5-13409) on Native Human LS174T Cells.
Publications:
Western Blot
  Species / Dilution Summary
  Hu / 0
MA5-13409 was used in immunocytochemistry and western blot to investigate the influence of XRCC1 on camptothecin resistance

Cancer Res. 2002 Jan 15;62(2):459-65.
"X-ray repair cross-complementing gene I protein plays an important role in camptothecin resistance."
Author(s): Park SY, Lam W, Cheng YC
Number of Citations: 5
(See PubMed article )
  Hu / 1:500
MA5-13409 was used in immunocytochemistry and western blot to investigate the effect of XRCC1(R280H) variant protein on DNA repair

Mutat Res. 2005 Apr 4;582(1-2):135-45.
"The Arg280His polymorphism in X-ray repair cross-complementing gene 1 impairs DNA repair ability."
Author(s): Takanami T, Nakamura J, Kubota Y, Horiuchi S
Number of Citations: 1
(See PubMed article )
  Hu / 1:150
MA5-13409 was used in western blot to investigate the relation between XRCC1 expression and apoptosis in breast cancer cell lines

Breast Cancer Res Treat. 2006 Oct;99(3):257-65.
"An intronic polymorphism associated with increased XRCC1 expression, reduced apoptosis and familial breast cancer."
Author(s): Bu D, Tomlinson G, Lewis CM, Zhang C, Kildebeck E, Euhus DM
Number of Citations: 1
(See PubMed article )
  Hu / 0
MA5-13409 was used in western blot to study the role of Chk2-dependent XRCC1 phosphorylation in the DNA damage response

EMBO J. 2008 Dec 3;27(23):3140-50.
"Chk2-dependent phosphorylation of XRCC1 in the DNA damage response promotes base excision repair."
Author(s): Chou WC, Wang HC, Wong FH, Ding SL, Wu PE, Shieh SY, Shen CY
Number of Citations: 7
(See PubMed article )
Immunocytochemistry
  Species / Dilution Summary
  Hu / 1:200
MA5-13409 was used in immunocytochemistry and western blot to investigate the influence of XRCC1 on camptothecin resistance

Cancer Res. 2002 Jan 15;62(2):459-65.
"X-ray repair cross-complementing gene I protein plays an important role in camptothecin resistance."
Author(s): Park SY, Lam W, Cheng YC
Number of Citations: 5
(See PubMed article )
  Hu / 1:50
MA5-13409 was used in immunocytochemistry to study the mammalian repair mechanism for oxidation-induced DNA damage

Proc Natl Acad Sci U S A. 2004 Sep 21;101(38):13738-43.
"In situ analysis of repair processes for oxidative DNA damage in mammalian cells."
Author(s): Lan L, Nakajima S, Oohata Y, Takao M, Okano S, Masutani M, Wilson SH, Yasui A
Number of Citations: 58
(See PubMed article )
  Hu / 1:500
MA5-13409 was used in immunocytochemistry and western blot to investigate the effect of XRCC1(R280H) variant protein on DNA repair

Mutat Res. 2005 Apr 4;582(1-2):135-45.
"The Arg280His polymorphism in X-ray repair cross-complementing gene 1 impairs DNA repair ability."
Author(s): Takanami T, Nakamura J, Kubota Y, Horiuchi S
Number of Citations: 1
(See PubMed article )
Immunohistochemistry
  Species / Dilution Summary
  Hu / 1:300
MA5-13409 was used in immunohistochemistry to investigate the usefulness of specific gene expression in non-small cell lung cancer treatment prognosis

Lung Cancer. 2010 Jun;68(3):478-83.
"The prognostic significance of ERCC1, BRCA1, XRCC1, and betaIII-tubulin expression in patients with non-small cell lung cancer treated by platinum- and taxane-based neoadjuvant chemotherapy and surgical resection."
Author(s): Kang CH, Jang BG, Kim DW, Chung DH, Kim YT, Jheon S, Sung SW, Kim JH
Number of Citations: 1
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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