XRCC1 Antibody, Biotin conjugate (33-2-5)

XRCC1 Monoclonal Antibody for Western blot, IHC (P), IP

>> See 5 other antibodies for XRCC1 (DNA repair protein XRCC1, X-ray repair cross-complementing protein 1, X-ray-repair, complementing defective, repair in Chinese hamster)
Synonyms:
DNA repair protein XRCC1, X-ray repair cross-complementing protein 1, X-ray-repair, complementing defective, repair in Chinese hamster
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Mouse / IgG2b
Class: Monoclonal
Type: Antibody
Clone: 33-2-5
Label: Biotin
Tested Species Reactivity: Human (Hu), Rat (Rt)
Published Species Reactivity: Human (Hu), Rabbit (Rb)
Immunogen: Recombinant human XRCC1 protein
Ordering Information
Pierce XRCC1 Antibody, Biotin conjugate (33-2-5)
Product #
MA5-13409
Size
500 ul
Price
$425.00
Purchase
Add XRCC1 Antibody, Biotin conjugate (33-2-5) to your cart.
Tested Applications Dilution *
Western Blot (WB) 1-2 µg/ml
Immunohistochemistry (Paraffin) (IHC (P)) 1-2 µg/ml
Immunoprecipitation (IP) 2 µg/ml
Published Applications Dilution
Western Blot (WB) See publications below
Immunocytochemistry (ICC) See publications below
Immunohistochemistry (IHC) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 0.2mg/ml
Purification: Protein A
Storage Buffer: PBS, pH 7.4, with 0.2% BSA
Preservative: 0.09% sodium azide
Storage Conditions: 4° C
Product Specific Information
MA5-13409 targets XRCC1 in IHC (P), IP, and WB applications and shows reactivity with Human and Rat samples.

The MA5-13409 immunogen is recombinant human XRCC1 protein.
General Information
X-Ray Repair Cross Complementing (XRCC1) plays a role in excision repair of DNA after ionizing irradiation. XRCC1 binds to DNA ligase III through the C-terminal 96 amino acids and to DNA polymerase beta through the N-terminal half. In testis XRCC1 is expressed at high levels. Cells with a mutation of this gene exhibit decreased single strand break repair and reduced recombination repair. They show increased double strand breaks, and sister chromatid exchange is increased up to 10-fold. The XRCC1 might serve as a scaffold protein during base excision-repair.
Product Images
  • XRCC1 Antibody (MA5-13409) in WB
    MA5-13409_WesternBlot.jpg

    Western Blot with anti-XRCC1 Monoclonal Antibody [33-2-5], Biotin conjugate (MA5-13409) (MA5-13409_WesternBlot.jpg)

    Western Blot with anti-XRCC1 Monoclonal Antibody [33-2-5], Biotin conjugate (MA5-13409)

    Western blot of XRCC1 using XRCC1 Monoclonal Antibody (MA5-13409) on LS174T Cells.
  • XRCC1 Antibody (MA5-13409) in IHC
    MA5-13409_Immunohistochemistry.jpg

    Immunohistochemistry with anti-XRCC1 Monoclonal Antibody [33-2-5], Biotin conjugate (MA5-13409) (MA5-13409_Immunohistochemistry.jpg)

    Immunohistochemistry with anti-XRCC1 Monoclonal Antibody [33-2-5], Biotin conjugate (MA5-13409)

    Formalin-fixed, paraffin-embedded human testis stained with XRCC1 antibody using peroxidase-conjugate and AEC chromogen. Note nuclear staining of spermatocytes.
  • XRCC1 Antibody (MA5-13409) in IHC
    MA5-13409_Immunohistochemistry2.jpg

    Immunohistochemistry with anti-XRCC1 Monoclonal Antibody [33-2-5], Biotin conjugate (MA5-13409) (MA5-13409_Immunohistochemistry2.jpg)

    Immunohistochemistry with anti-XRCC1 Monoclonal Antibody [33-2-5], Biotin conjugate (MA5-13409)

    Formalin-fixed, paraffin-embedded rat testis stained with XRCC1 antibody using peroxidase-conjugate and AEC chromogen. Note nuclear staining of spermatocytes.
  • XRCC1 Antibody (MA5-13409) in IHC
    MA5-13409_Immunohistochemistry_2.jpg

    Immunohistochemistry with anti-XRCC1 Monoclonal Antibody [33-2-5], Biotin conjugate (MA5-13409) (MA5-13409_Immunohistochemistry_2.jpg)

    Immunohistochemistry with anti-XRCC1 Monoclonal Antibody [33-2-5], Biotin conjugate (MA5-13409)

  • XRCC1 Antibody (MA5-13409) in IP
    MA5-13409_Immunoprecipitation.jpg

    Immunoprecipitation with anti-XRCC1 Monoclonal Antibody [33-2-5], Biotin conjugate (MA5-13409) (MA5-13409_Immunoprecipitation.jpg)

    Immunoprecipitation with anti-XRCC1 Monoclonal Antibody [33-2-5], Biotin conjugate (MA5-13409)

    Immunoprecipitation of XRCC1 using XRCC1 Monoclonal Antibody (MA5-13409) on Native Human LS174T Cells.
Publications:
Western Blot
  Species / Dilution Summary
  Hu / 0
MA5-13409 was used in immunocytochemistry and western blot to investigate the influence of XRCC1 on camptothecin resistance

Cancer Res. 2002 Jan 15;62(2):459-65.
"X-ray repair cross-complementing gene I protein plays an important role in camptothecin resistance."
Author(s): Park SY, Lam W, Cheng YC
Number of Citations: 5
(See PubMed article )
  Hu / Not Cited
MA5-13409 was used in western blot to study an alternative pathway for the repair of DNA double-strand breaks that involves poly(ADP-ribose) polymerase-1 and a XRCC1-DNA ligase III complex

J Biol Chem. 2004 Dec 31;279(53):55117-26.
"Involvement of poly(ADP-ribose) polymerase-1 and XRCC1/DNA ligase III in an alternative route for DNA double-strand breaks rejoining."
Author(s): Audebert M, Salles B, Calsou P
Number of Citations: 118
(See PubMed article )
  Hu / 1:500
MA5-13409 was used in immunocytochemistry and western blot to investigate the effect of XRCC1(R280H) variant protein on DNA repair

Mutat Res. 2005 Apr 4;582(1-2):135-45.
"The Arg280His polymorphism in X-ray repair cross-complementing gene 1 impairs DNA repair ability."
Author(s): Takanami T, Nakamura J, Kubota Y, Horiuchi S
Number of Citations: 1
(See PubMed article )
  Hu / 1:150
MA5-13409 was used in western blot to investigate the relation between XRCC1 expression and apoptosis in breast cancer cell lines

Breast Cancer Res Treat. 2006 Oct;99(3):257-65.
"An intronic polymorphism associated with increased XRCC1 expression, reduced apoptosis and familial breast cancer."
Author(s): Bu D, Tomlinson G, Lewis CM, Zhang C, Kildebeck E, Euhus DM
Number of Citations: 1
(See PubMed article )
  Hu / 0
MA5-13409 was used in western blot to study the role of Chk2-dependent XRCC1 phosphorylation in the DNA damage response

EMBO J. 2008 Dec 3;27(23):3140-50.
"Chk2-dependent phosphorylation of XRCC1 in the DNA damage response promotes base excision repair."
Author(s): Chou WC, Wang HC, Wong FH, Ding SL, Wu PE, Shieh SY, Shen CY
Number of Citations: 7
(See PubMed article )
  Hu / 1:1000
MA5-13409 was used in western blot to study the subcellular distribution of a mixed EGFR-DNA targeting molecule utilizing a green labelled DNA-damaging moiety and a blue-labelled EGFR inhibitor

Mol Cancer Ther. 2010 Apr;9(4):869-82.
"Subcellular distribution of a fluorescence-labeled combi-molecule designed to block epidermal growth factor receptor tyrosine kinase and damage DNA with a green fluorescent species."
Author(s): Todorova MI, Larroque AL, Dauphin-Pierre S, Fang YQ, Jean-Claude BJ
Number of Citations: 0
(See PubMed article )
  Hu / Not Cited
MA5-13409 was used in immunohistochemistry and western blot to study the hypersensitivity XRCC1-deficient breast cancer to inhibitors of double-strand break repair

Cancer Res. 2013 Mar 1;73(5):1621-34.
"Targeting XRCC1 deficiency in breast cancer for personalized therapy."
Author(s): Sultana R, Abdel-Fatah T, Abbotts R, Hawkes C, Albarakati N, Seedhouse C, Ball G, Chan S, Rakha EA, Ellis IO, Madhusudan S
Number of Citations: 3
(See PubMed article )
  Rb / Not Cited
MA5-13409 was used in western blot to study the effects of dietary lipid levels on DNA damage and DNA repair pathways in experimentally induced atherosclerotic plaques

Circ Res. 2001 Apr 13;88(7):733-9.
"Oxidative DNA damage and repair in experimental atherosclerosis are reversed by dietary lipid lowering."
Author(s): Martinet W, Knaapen MW, De Meyer GR, Herman AG, Kockx MM
Number of Citations: 13
(See PubMed article )
Immunocytochemistry
  Species / Dilution Summary
  Hu / 1:200
MA5-13409 was used in immunocytochemistry and western blot to investigate the influence of XRCC1 on camptothecin resistance

Cancer Res. 2002 Jan 15;62(2):459-65.
"X-ray repair cross-complementing gene I protein plays an important role in camptothecin resistance."
Author(s): Park SY, Lam W, Cheng YC
Number of Citations: 5
(See PubMed article )
  Hu / 1:50
MA5-13409 was used in immunocytochemistry to study the mammalian repair mechanism for oxidation-induced DNA damage

Proc Natl Acad Sci U S A. 2004 Sep 21;101(38):13738-43.
"In situ analysis of repair processes for oxidative DNA damage in mammalian cells."
Author(s): Lan L, Nakajima S, Oohata Y, Takao M, Okano S, Masutani M, Wilson SH, Yasui A
Number of Citations: 58
(See PubMed article )
  Hu / 1:500
MA5-13409 was used in immunocytochemistry and western blot to investigate the effect of XRCC1(R280H) variant protein on DNA repair

Mutat Res. 2005 Apr 4;582(1-2):135-45.
"The Arg280His polymorphism in X-ray repair cross-complementing gene 1 impairs DNA repair ability."
Author(s): Takanami T, Nakamura J, Kubota Y, Horiuchi S
Number of Citations: 1
(See PubMed article )
Immunohistochemistry
  Species / Dilution Summary
  Hu / 1:300
MA5-13409 was used in immunohistochemistry to investigate the usefulness of specific gene expression in non-small cell lung cancer treatment prognosis

Lung Cancer. 2010 Jun;68(3):478-83.
"The prognostic significance of ERCC1, BRCA1, XRCC1, and betaIII-tubulin expression in patients with non-small cell lung cancer treated by platinum- and taxane-based neoadjuvant chemotherapy and surgical resection."
Author(s): Kang CH, Jang BG, Kim DW, Chung DH, Kim YT, Jheon S, Sung SW, Kim JH
Number of Citations: 1
(See PubMed article )
  Hu / 1:200
MA5-13409 was used in immunohistochemistry to study the function and predictive value of XRCC1 in ovarian cancer

Int J Cancer. 2013 Jun 15;132(12):2778-86.
"Clinicopathological and functional significance of XRCC1 expression in ovarian cancer."
Author(s): Abdel-Fatah T, Sultana R, Abbotts R, Hawkes C, Seedhouse C, Chan S, Madhusudan S
Number of Citations: 1
(See PubMed article )
  Hu / 1:200
MA5-13409 was used in immunohistochemistry and western blot to study the hypersensitivity XRCC1-deficient breast cancer to inhibitors of double-strand break repair

Cancer Res. 2013 Mar 1;73(5):1621-34.
"Targeting XRCC1 deficiency in breast cancer for personalized therapy."
Author(s): Sultana R, Abdel-Fatah T, Abbotts R, Hawkes C, Albarakati N, Seedhouse C, Ball G, Chan S, Rakha EA, Ellis IO, Madhusudan S
Number of Citations: 3
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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