XPA Antibody (12F5)

XPA Monoclonal Antibody for Western blot, IHC (P)

>> See 4 other antibodies for XPA (DNA repair protein complementing XP-A cells, excision repair-controlling, xeroderma pigmentosum group A-complementing protein)
Synonyms:
DNA repair protein complementing XP-A cells, excision repair-controlling, xeroderma pigmentosum group A-complementing protein
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Mouse / IgG2a, kappa
Class: Monoclonal
Type: Antibody
Clone: 12F5
Tested Species Reactivity: Human (Hu)
Published Species Reactivity: Bovine (Bv), Human (Hu)
Immunogen: Recombinant human XPA protein
Ordering Information
Pierce XPA Antibody (12F5)
Product #
MA5-13835
Size
500 ul
Price
$316.00
Purchase
Add XPA Antibody (12F5) to your cart.
Tested Applications Dilution *
Western Blot (WB) 1-2 µg/ml
Immunohistochemistry (Paraffin) (IHC (P)) 2-4 µg/ml
Published Applications Dilution
Western Blot (WB) See publications below
Immunocytochemistry (ICC) See publications below
Immunohistochemistry (IHC) See publications below
Immunoprecipitation (IP) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 0.2mg/ml
Purification: Protein A
Storage Buffer: PBS, pH 7.4, with 0.2% BSA
Preservative: 0.09% sodium azide
Storage Conditions: 4° C
Product Specific Information
MA5-13835 targets XPA in IHC (P) and WB applications and shows reactivity with Human samples.

The MA5-13835 immunogen is recombinant human XPA protein.
General Information
The XPA (xeroderma pigmentosum group A) protein specifically recognizes the UV-or chemically damaged DNA lesions, and triggers the nucleotide excision repair process. XPA binds to the replication protein A (RPA) or the excision repair cross complementing 1 protein (ERCC1). In the absence of nucleotide excision repair persisting (unrepaired) DNA lesions (adducts) may lead to the accumulation of gene mutations and ultimately to cancer. Xeroderma pigmentosum patients have a > 2000 fold increased risk to develop skin cancer at sun-exposed areas.
Product Images
  • XPA Antibody (MA5-13835) in WB
    MA5-13835_WesternBlot.jpg

    Western Blot with anti-XPA Monoclonal Antibody [12F5] (MA5-13835) (MA5-13835_WesternBlot.jpg)

    Western Blot with anti-XPA Monoclonal Antibody [12F5] (MA5-13835)

    Western blot of XPA using XPA Monoclonal Antibody (MA5-13835) on LS174T Cells.
  • XPA Antibody (MA5-13835) in IHC
    MA5-13835_Immunohistochemistry.jpg

    Immunohistochemistry with anti-XPA Monoclonal Antibody [12F5] (MA5-13835) (MA5-13835_Immunohistochemistry.jpg)

    Immunohistochemistry with anti-XPA Monoclonal Antibody [12F5] (MA5-13835)

    Formalin-fixed, paraffin-embedded human tonsil stained with XPA antibody using peroxidase-conjugate and AEC chromogen. Note nuclear staining of epithelial cells.
Publications:
Western Blot
  Species / Dilution Summary
  Bv / Not Cited
MA5-13835 was used in western blot to study the ability of replication protein to recognize and bind to cisplatin-DNA interstrand cross-links

Biochemistry. 2008 Sep 23;47(38):10188-96.
"Recognition of cisplatin-DNA interstrand cross-links by replication protein A."
Author(s): Patrick SM, Tillison K, Horn JM
Number of Citations: 5
(See PubMed article )
  Hu / 1:200
MA5-13835 was used in western blot to investigate the molecular mechanisms of spontaneous mesenchymal stem cell transformation

PLoS One. 2008 Jan 2;3(1):e1398.
"Molecular characterization of spontaneous mesenchymal stem cell transformation."
Author(s): Rubio D, Garcia S, Paz MF, De la Cueva T, Lopez-Fernandez LA, Lloyd AC, Garcia-Castro J, Bernad A
Number of Citations: 14
(See PubMed article )
  Hu / 0
MA5-13835 was used in immunocytochemistry and western blot to study the mechanism by which GCN5 acetyltransferase and E2F1 stimulate nucleotide excission repair

Nucleic Acids Res. 2011 Mar;39(4):1390-7.
"GCN5 and E2F1 stimulate nucleotide excision repair by promoting H3K9 acetylation at sites of damage."
Author(s): Guo R, Chen J, Mitchell DL, Johnson DG
Number of Citations: 9
(See PubMed article )
Immunocytochemistry
  Species / Dilution Summary
  Hu / 0
MA5-13835 was used in immunocytochemistry to study the role of ubiquitinated H2A in chromatin restoration after nucleotide excision repair

DNA Repair (Amst). 2009 Feb 1;8(2):262-73.
"Chromatin restoration following nucleotide excision repair involves the incorporation of ubiquitinated H2A at damaged genomic sites."
Author(s): Zhu Q, Wani G, Arab HH, El-Mahdy MA, Ray A, Wani AA
Number of Citations: 9
(See PubMed article )
  Hu / Not Cited
MA5-13835 was used in immunocytochemistry to study the functional association between XP-G/CS mutations and the disassembly state of TFIIH

PLoS One. 2010 Jun 8;5(6):e11007.
"Dissociation of CAK from core TFIIH reveals a functional link between XP-G/CS and the TFIIH disassembly state."
Author(s): Arab HH, Wani G, Ray A, Shah ZI, Zhu Q, Wani AA
Number of Citations: 6
(See PubMed article )
  Hu / 0
MA5-13835 was used in immunocytochemistry and western blot to study the mechanism by which GCN5 acetyltransferase and E2F1 stimulate nucleotide excission repair

Nucleic Acids Res. 2011 Mar;39(4):1390-7.
"GCN5 and E2F1 stimulate nucleotide excision repair by promoting H3K9 acetylation at sites of damage."
Author(s): Guo R, Chen J, Mitchell DL, Johnson DG
Number of Citations: 9
(See PubMed article )
  Hu / Not Cited
MA5-13835 was used in immunocytochemistry to study the role of the XPB and XPD helicases in regulating the anchorage of the CAK complex to TFIIH during nucleotide excision repair

DNA Repair (Amst). 2012 Dec 1;11(12):942-50.
"Lack of CAK complex accumulation at DNA damage sites in XP-B and XP-B/CS fibroblasts reveals differential regulation of CAK anchoring to core TFIIH by XPB and XPD helicases during nucleotide excision repair."
Author(s): Zhu Q, Wani G, Sharma N, Wani A
Number of Citations: 0
(See PubMed article )
Immunohistochemistry
  Species / Dilution Summary
  Hu / 1:200
MA5-13835 was used in immunohistochemistry to evaluate the prognostic value of xeroderma pigmentosum A protein expression in metastatic ovarian carcinoma

Cancer. 2005 Jun 1;103(11):2313-9.
"Expression of xeroderma pigmentosum A protein predicts improved outcome in metastatic ovarian carcinoma."
Author(s): Stevens EV, Raffeld M, Espina V, Kristensen GB, Trope' CG, Kohn EC, Davidson B
Number of Citations: 1
(See PubMed article )
Immunoprecipitation
  Species / Dilution Summary
  Hu / 0
MA5-13835 was used in immunoprecipitation to study the interaction of DNA polymerase epsilon with the elongating form of RNA polymerase II

FEBS J. 2006 Dec;273(24):5535-49.
"DNA polymerase epsilon associates with the elongating form of RNA polymerase II and nascent transcripts."
Author(s): Rytkönen AK, Hillukkala T, Vaara M, Sokka M, Jokela M, Sormunen R, Nasheuer HP, Nethanel T, Kaufmann G, Pospiech H, Syväoja JE
Number of Citations: 1
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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