XPA Antibody (12F5)

XPA Monoclonal Antibody for Western blot, IHC (P)

>> See 4 other antibodies for XPA (DNA repair protein complementing XP-A cells, excision repair-controlling, xeroderma pigmentosum group A-complementing protein)
Synonyms:
DNA repair protein complementing XP-A cells, excision repair-controlling, xeroderma pigmentosum group A-complementing protein
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Mouse / IgG2a, kappa
Class: Monoclonal
Type: Antibody
Clone: 12F5
Tested Species Reactivity: Human (Hu)
Published Species Reactivity: Human (Hu)
Immunogen: Recombinant human XPA protein
Ordering Information
Pierce XPA Antibody (12F5)
Product #
MA5-13835
Size
500 ul
Price
$316.00
Purchase
Add XPA Antibody (12F5) to your cart.
Tested Applications Dilution *
Western Blot (WB) 1-2 µg/ml
Immunohistochemistry (Paraffin) (IHC (P)) 2-4 µg/ml
Published Applications Dilution
Western Blot (WB) See publications below
Immunocytochemistry (ICC) See publications below
Immunohistochemistry (IHC) See publications below
Immunoprecipitation (IP) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 0.2mg/ml
Purification: Protein A
Storage Buffer: PBS, pH 7.4, with 0.2% BSA
Preservative: 0.09% sodium azide
Storage Conditions: 4° C
Product Specific Information
MA5-13835 targets XPA in IHC (P) and WB applications and shows reactivity with Human samples.

The MA5-13835 immunogen is recombinant human XPA protein.
General Information
The XPA (xeroderma pigmentosum group A) protein specifically recognizes the UV-or chemically damaged DNA lesions, and triggers the nucleotide excision repair process. XPA binds to the replication protein A (RPA) or the excision repair cross complementing 1 protein (ERCC1). In the absence of nucleotide excision repair persisting (unrepaired) DNA lesions (adducts) may lead to the accumulation of gene mutations and ultimately to cancer. Xeroderma pigmentosum patients have a > 2000 fold increased risk to develop skin cancer at sun-exposed areas.
Product Images
  • XPA Antibody (MA5-13835) in WB
    MA5-13835_WesternBlot.jpg

    Western Blot with anti-XPA Monoclonal Antibody [12F5] (MA5-13835) (MA5-13835_WesternBlot.jpg)

    Western Blot with anti-XPA Monoclonal Antibody [12F5] (MA5-13835)

    Western blot of XPA using XPA Monoclonal Antibody (MA5-13835) on LS174T Cells.
  • XPA Antibody (MA5-13835) in IHC
    MA5-13835_Immunohistochemistry.jpg

    Immunohistochemistry with anti-XPA Monoclonal Antibody [12F5] (MA5-13835) (MA5-13835_Immunohistochemistry.jpg)

    Immunohistochemistry with anti-XPA Monoclonal Antibody [12F5] (MA5-13835)

    Formalin-fixed, paraffin-embedded human tonsil stained with XPA antibody using peroxidase-conjugate and AEC chromogen. Note nuclear staining of epithelial cells.
Publications:
Western Blot
  Species / Dilution Summary
  Hu / 1:200
MA5-13835 was used in western blot to investigate the molecular mechanisms of spontaneous mesenchymal stem cell transformation

PLoS One. 2008 Jan 2;3(1):e1398.
"Molecular characterization of spontaneous mesenchymal stem cell transformation."
Author(s): Rubio D, Garcia S, Paz MF, De la Cueva T, Lopez-Fernandez LA, Lloyd AC, Garcia-Castro J, Bernad A
Number of Citations: 14
(See PubMed article )
  Hu / 0
MA5-13835 was used in immunocytochemistry and western blot to study the mechanism by which GCN5 acetyltransferase and E2F1 stimulate nucleotide excission repair

Nucleic Acids Res. 2011 Mar;39(4):1390-7.
"GCN5 and E2F1 stimulate nucleotide excision repair by promoting H3K9 acetylation at sites of damage."
Author(s): Guo R, Chen J, Mitchell DL, Johnson DG
Number of Citations: 9
(See PubMed article )
Immunocytochemistry
  Species / Dilution Summary
  Hu / 0
MA5-13835 was used in immunocytochemistry to study the role of ubiquitinated H2A in chromatin restoration after nucleotide excision repair

DNA Repair (Amst). 2009 Feb 1;8(2):262-73.
"Chromatin restoration following nucleotide excision repair involves the incorporation of ubiquitinated H2A at damaged genomic sites."
Author(s): Zhu Q, Wani G, Arab HH, El-Mahdy MA, Ray A, Wani AA
Number of Citations: 9
(See PubMed article )
  Hu / 0
MA5-13835 was used in immunocytochemistry and western blot to study the mechanism by which GCN5 acetyltransferase and E2F1 stimulate nucleotide excission repair

Nucleic Acids Res. 2011 Mar;39(4):1390-7.
"GCN5 and E2F1 stimulate nucleotide excision repair by promoting H3K9 acetylation at sites of damage."
Author(s): Guo R, Chen J, Mitchell DL, Johnson DG
Number of Citations: 9
(See PubMed article )
Immunohistochemistry
  Species / Dilution Summary
  Hu / 1:200
MA5-13835 was used in immunohistochemistry to evaluate the prognostic value of xeroderma pigmentosum A protein expression in metastatic ovarian carcinoma

Cancer. 2005 Jun 1;103(11):2313-9.
"Expression of xeroderma pigmentosum A protein predicts improved outcome in metastatic ovarian carcinoma."
Author(s): Stevens EV, Raffeld M, Espina V, Kristensen GB, Trope' CG, Kohn EC, Davidson B
Number of Citations: 1
(See PubMed article )
Immunoprecipitation
  Species / Dilution Summary
  Hu / 0
MA5-13835 was used in immunoprecipitation to study the interaction of DNA polymerase epsilon with the elongating form of RNA polymerase II

FEBS J. 2006 Dec;273(24):5535-49.
"DNA polymerase epsilon associates with the elongating form of RNA polymerase II and nascent transcripts."
Author(s): Rytkönen AK, Hillukkala T, Vaara M, Sokka M, Jokela M, Sormunen R, Nasheuer HP, Nethanel T, Kaufmann G, Pospiech H, Syväoja JE
Number of Citations: 1
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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