XCT Antibody

XCT Polyclonal Antibody for Western blot, IF, ICC, IHC (P), FACS

>> See 4 other antibodies for SLC7A11 (Cysteine/glutamate transporter antibody, Amino acid transport system xc-xCT antibody, Calcium channel blocker resistance protein CCBR1 antibody.)
Synonyms:
Cysteine/glutamate transporter antibody, Amino acid transport system xc-xCT antibody, Calcium channel blocker resistance protein CCBR1 antibody.
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Rabbit
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu), Mouse (Ms), Rat (Rt)
Immunogen: Synthetic peptide between amino acid 1-50 of the human XCT protein.
Ordering Information
Pierce XCT Antibody
Product #
PA1-16893
Size
100 µl
Price
$350.00
Purchase
Add XCT Antibody to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:1000
Immunofluorescence (IF) 1:100-1:1000
Immunocytochemistry (ICC) 1:100-1:1000
Immunohistochemistry (Paraffin) (IHC (P)) 5 µg/ml
Flow Cytometry (FACS) 2-3 µg/ml
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 1mg/ml
Purification: Antigen affinity chromatography
Storage Buffer: tris glycine with 0.15M NaCl
Preservative: 0.05% sodium azide
Storage Conditions: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Product Specific Information
This antibody is not recommended for Immunoprecipitation.

Permeablization is recommended prior to performing Flow analysis.

Suggested positive control: antigen standard for SLC7A11 (transient overexpression lysate), human stomach protein or stomach tissue lysate.
General Information
Mammalian amino acid transport systems consist of a large variety of transporters. The amino acid transporter, cystine/glutamic acid transporter xCT, for system xc has been proposed to be responsible for the cystine transport through the plasma membrane. System xc mediates an amino acid exchange and prefers cystine and glutamate as its substrates. The transporter designated as xCT requires 4F2 heavy chain (4F2hc:CD98) for its functional expression, and belongs to the family of amino acid transporters that associates with the type II membrane glycoproteins such as 4F2hc.

In brain, it has been proposed that system xc is up-regulated in glial cells upon oxidative stress and plays an essential roles to protect neurons against oxidative stress. Recent studies have implicated xCT as a gateway that Kaposi's sarcoma herpesvirus (KSHV) uses to enter cells.
Product Images
  • XCT Antibody (PA1-16893) in WB
    PA1-16893_Westernblot.jpg

    Western Blot with anti-XCT Polyclonal Antibody (PA1-16893) (PA1-16893_Westernblot.jpg)

    Western Blot with anti-XCT Polyclonal Antibody (PA1-16893)

    Western Blot detection of XCT in total human and mouse stomach lysate, respectively, using PA1-16893. 1 minute ECL exposure.
  • XCT Antibody (PA1-16893) in ICC
    PA1-16893_Immunoctyochemistry.jpg

    Immunocytochemistry with anti-XCT Polyclonal Antibody (PA1-16893) (PA1-16893_Immunoctyochemistry.jpg)

    Immunocytochemistry with anti-XCT Polyclonal Antibody (PA1-16893)

    Detection of XCT (Green) in HepG2 cells using PA1-16893. Nuclei (Blue) are counterstained with Hoechst 33258.
  • XCT Antibody (PA1-16893) in IHC
    PA1-16893_Immunohistochemistry.jpg

    Immunohistochemistry with anti-XCT Polyclonal Antibody (PA1-16893) (PA1-16893_Immunohistochemistry.jpg)

    Immunohistochemistry with anti-XCT Polyclonal Antibody (PA1-16893)

    XCT staining in the absorptive epithelia of intestinal villi detected using PA1-16893.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific