Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
100 ul of Antigen affinity chromatography purified antibody (1 mg/ml) in Tris glycine, 0.15M NaCl with 0.05% Sodium azide.
Western Blot (WB)
1:100 - 1:1000
Immunohistochemistry (Paraffin) (IHC (P))
Flow Cytometry (FACS)
1:100 - 1:1000
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
PA1-16893 detects XCT protein in human, rat and mouse samples.
PA1-16893 has been successfully used in flow cytometry, Western blot, immunocytochemistry, immunofluorescence and immunohistochemistry (FFPE) procedures. PA1-16893 is specific for the ~55 kDa XCT protein. This antibody is not recommended for use in immunoprecipitation procedures.
PA1-16893 immunogen is a synthetic peptide between amino acid 1-50 of the human XCT protein.
Store at 4ºC short term. For extended storage, aliquot and store at -20ºC, avoiding freeze/thaw cycles.
Mammalian amino acid transport systems consist of a large variety of transporters. The amino acid transporter, cystine/glutamic acid transporter xCT, for system xc has been proposed to be responsible for the cystine transport through the plasma membrane. System xc mediates an amino acid exchange and prefers cystine and glutamate as its substrates. The transporter designated as xCT requires 4F2 heavy chain (4F2hc:CD98) for its functional expression, and belongs to the family of amino acid transporters that associates with the type II membrane glycoproteins such as 4F2hc.
In brain, it has been proposed that system xc is up-regulated in glial cells upon oxidative stress and plays an essential roles to protect neurons against oxidative stress. Recent studies have implicated xCT as a gateway that Kaposi's sarcoma herpesvirus (KSHV) uses to enter cells.
XCT Antibody (PA1-16893) in ICC
Immunocytochemistry with anti-XCT Polyclonal Antibody (PA1-16893)