Part of Thermo Fisher Scientific

Vitronectin Monoclonal Antibody (HV2)

Vitronectin Antibody for ELISA, IHC, Western blot

Synonyms:
Serum spreading factor, Complement S Protein, Epibolin, S Protein, Somatomedin B antibody, V75, VTN
Entrez Gene ID:
(Human) 7448
UniProt ID:
P04004
Details
Host / Isotype: Mouse / IgG2b
Class: Monoclonal
Type: Antibody
Clone: HV2
Species Reactivity: Human (Hu)
Immunogen: Human vitronectin purified from plasma by heparin-affinity chromatography.
Ordering Information
Pierce Vitronectin Monoclonal Antibody (HV2)
Product Number Pkg. Size Price Purchase
CSI 003-02-02 200 µg $362.00
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Storage: 4º C, store in dark
Form: 200 µg of Protein A/G purified antibody (1 mg/ml) in 0.01 M phosphate buffer, with 0.5 M NaCl and 15 mM sodium azide; pH 7.4.


Applications Dilution *
Immunohistochemistry (IHC) Assay Dependent
Western Blot (WB) 1:100
ELISA (ELISA) 1:32,000
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
CSI 003-02-02 is highly specific for vitronectin. There is no evidence for cross-reactivity with other connective tissue proteins (fibronectin, elastin, collagen, laminin). CSI 003-02-02 is human-specific (no reactivity with cat, dog, cow, sheep, goat, pig, rabbit, horse).

CSI 003-02-02 has been successfully used in ELISA, immunohistochemistry, and Western blotting techniques. CSI 003-02-02 can be used to purify vitronectin from human plasma by affinity chromatography. It can also be used to quantitatively affinity-deplete human plasma or serum of vitronectin. It partially denatures vitronectin upon antibody binding. It binds equally well to native and denatured vitronectin and can be used to quantitate vitronectin in human plasma in a sandwich ELISA with CSI 003-02-02. It does not interfere with the binding of any known vitronectin ligands.

The CSI 003-02-02 immunogen is human vitronectin purified from plasma by heparin-affinity chromatography.

The epitope of CSI 003-02-02 is located in the connecting region. Binding can be competed selectively by a peptide comprising aa 121-133.
General Information
Vitronectin is a plasma glycoprotein that circulates in the blood. Vitronectin is circulating as a mixture of both 75 kDa and 65 kDa forms. Vitronectin is a major cell adhesive glycoprotein and is a common component of extracellular matrix and plasma. It competes effectively with other plasma proteins and is often involved in cell attachment, regulation of blood coagulation and immune responses. It has similar tissue distribution to fibronectin and also its integrin receptor recognises fibronectin.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)