Vinculin Antibody (VLN01)

Vinculin Monoclonal Antibody for Western blot, IF, IHC (P), IP

>> See 6 other antibodies for VCL ( metavinculin)
Synonyms:
metavinculin
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Mouse / IgG
Class: Monoclonal
Type: Antibody
Clone: VLN01
Tested Species Reactivity: Human (Hu)
Published Species Reactivity: Feline (Fe), Human (Hu)
Immunogen: Semi-purified vinculin
Ordering Information
Pierce Vinculin Antibody (VLN01)
Product #
MA5-11690
Size
500 ul
Price
$316.00
Purchase
Add Vinculin Antibody (VLN01) to your cart.
Tested Applications Dilution *
Western Blot (WB) 1-2 µg/ml
Immunofluorescence (IF) Assay Dependent
Immunohistochemistry (Paraffin) (IHC (P)) 1:50
Immunoprecipitation (IP) 2 µg/ml
Published Applications Dilution
Western Blot (WB) See publications below
Immunocytochemistry (ICC) See publications below
Immunohistochemistry (IHC) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 0.2mg/ml
Purification: Protein G
Storage Buffer: PBS, pH 7.4, with 0.2% BSA
Preservative: 0.09% sodium azide
Storage Conditions: 4° C, do not freeze
Product Specific Information
MA5-11690 targets Vinculin in IF, IHC (P), IP, and WB applications and shows reactivity with Human samples.

The MA5-11690 immunogen is semi-purified vinculin.
General Information
Vinculin is an attachment protein involved in the indirect binding of intracellular actin filaments to extracellular fibronectin. Vinculin binds to actin capping protein and alpha actinin, which in turn binds to actin. The binding of fibronectin is also indirect where vinculin binds to talin, which in turn binds to the intracellular domain of the fibronectin receptor which itself is the ligand for fibronectin. Vinculin is widely distributed in tissue and is expressed where smooth muscle actin and fibroblasts attach to the extracellular matrix.
Product Images
  • Vinculin Antibody (MA5-11690) in WB
    MA5-11690_WesternBlot.jpg

    Western Blot with anti-Vinculin Monoclonal Antibody [VLN01] (MA5-11690) (MA5-11690_WesternBlot.jpg)

    Western Blot with anti-Vinculin Monoclonal Antibody [VLN01] (MA5-11690)

    Western blot of Vinculin using Vinculin Monoclonal Antibody (MA5-11690) on HeLa Cells.
  • Vinculin Antibody (MA5-11690) in IP
    MA5-11690_Immunoprecipitation.jpg

    Immunoprecipitation with anti-Vinculin Monoclonal Antibody [VLN01] (MA5-11690) (MA5-11690_Immunoprecipitation.jpg)

    Immunoprecipitation with anti-Vinculin Monoclonal Antibody [VLN01] (MA5-11690)

    Immunoprecipitation of Vinculin using Vinculin Monoclonal Antibody (MA5-11690) on denatured Human LS174T Cells.
Publications:
Western Blot
  Species / Dilution Summary
  Hu / 0
MA5-11690 was used in western blot to investigate the influence of simvastatin on connective tissue growth factor expression and its mechanism

Am J Physiol Lung Cell Mol Physiol. 2004 Dec;287(6):L1323-32.
"Connective tissue growth factor expression and induction by transforming growth factor-beta is abrogated by simvastatin via a Rho signaling mechanism."
Author(s): Watts KL, Spiteri MA
Number of Citations: 1
(See PubMed article )
Immunocytochemistry
  Species / Dilution Summary
  Fe / Not Cited
MA5-11690 was used in immunocytochemistry to investigate the involvement of beta3 integrin and c-Src in focal adhesion complex formation in adult cardiomyocytes

J Mol Cell Cardiol. 2003 Jun;35(6):671-83.
"Focal complex formation in adult cardiomyocytes is accompanied by the activation of beta3 integrin and c-Src."
Author(s): Willey CD, Balasubramanian S, Rodríguez Rosas MC, Ross RS, Kuppuswamy D
Number of Citations: 6
(See PubMed article )
Immunohistochemistry
  Species / Dilution Summary
  Hu / 1:50
MA5-11690 was used in immunohistochemistry to investigate the involvement of focal adhesion plaque associated cytoskeletons in the invasion and metastasis of colorectal carcinoma

Cancer Invest. 2010 Feb;28(2):127-34.
"Focal adhesion plaque associated cytoskeletons are involved in the invasion and metastasis of human colorectal carcinoma."
Author(s): Yang HJ, Chen JZ, Zhang WL, Ding YQ
Number of Citations: 3
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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