VPS35 Antibody

VPS35 Polyclonal Antibody for Western blot, IHC (P)

>> See 2 other antibodies for VPS35 (MEM3, PARK17, hVPS35, maternal-embryonic 3, vacuolar protein sorting-associated protein 35)
Synonyms:
MEM3, PARK17, hVPS35, maternal-embryonic 3, vacuolar protein sorting-associated protein 35
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Rabbit / IgG
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu), Mouse (Ms)
Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 311 of Human VPS35
Ordering Information
Pierce VPS35 Antibody
Product #
PA5-30654
Size
100 µL
Price
$324.00
Purchase
Tested Applications Dilution *
Western Blot (WB) 1:500-1:3000
Immunohistochemistry (Paraffin) (IHC (P)) 1:100-1:1000
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 0.64mg/ml
Purification: Antigen affinity chromatography
Storage Buffer: 0.1M tris, pH 7, with 20% glycerol, 0.1M glycine
Preservative: 0.01% thimerosal
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
PA5-30654 targets VPS35 in IHC (P) and WB applications and shows reactivity with Human and mouse samples.

The PA5-30654 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 311 of Human VPS35.
General Information
This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex.
Product Images
  • VPS35 Antibody (PA5-30654) in WB
    PA5-30654_WB_1.jpg

    Western Blot with anti-VPS35 Polyclonal Antibody (PA5-30654) (PA5-30654_WB_1.jpg)

    Western Blot with anti-VPS35 Polyclonal Antibody (PA5-30654)

    Western blot analysis of VPS35 using 30µg of A) NIH-3T3 and B) JC lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a VPS35 polyclonal antibody (Product # PA5-30654) at a dilution of 1:1000.
  • VPS35 Antibody (PA5-30654) in WB
    PA5-30654_WB_2.jpg

    Western Blot with anti-VPS35 Polyclonal Antibody (PA5-30654) (PA5-30654_WB_2.jpg)

    Western Blot with anti-VPS35 Polyclonal Antibody (PA5-30654)

    Western blot analysis of VPS35 using 30µg of HCT116 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a VPS35 polyclonal antibody (Product # PA5-30654) at a dilution of 1:1000.
  • VPS35 Antibody (PA5-30654) in IHC (P)
    PA5-30654_IHC.jpg

    Immunohistochemistry (Paraffin) with anti-VPS35 Polyclonal Antibody (PA5-30654) (PA5-30654_IHC.jpg)

    Immunohistochemistry (Paraffin) with anti-VPS35 Polyclonal Antibody (PA5-30654)

    Immunohistochemical analysis of VPS35 in paraffin-embedded A549 xenograft using a VPS35 polyclonal antibody (Product # PA5-30654) at a dilution of 1:500.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific