* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Antigen affinity chromatography
0.1M tris, pH 7,
20% glycerol, 0.1M glycine
-20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
PA5-30654 targets VPS35 in IHC (P) and WB applications and shows reactivity with Human and mouse samples.
The PA5-30654 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 311 of Human VPS35.
This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex.
VPS35 Antibody (PA5-30654) in WB VPS35_PA5-30654_Antibody_WesternBlot.jpg
Western Blot with anti-VPS35 Polyclonal Antibody (PA5-30654)