VPS35 Antibody

VPS35 Polyclonal Antibody for Western blot, IF, ICC, IHC (P)

>> See 2 other antibodies for VPS35 (TCCCTA00141, MEM3, PARK17, hVPS35, maternal-embryonic 3, vacuolar protein sorting-associated protein 35)
Synonyms:
TCCCTA00141, MEM3, PARK17, hVPS35, maternal-embryonic 3, vacuolar protein sorting-associated protein 35
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Rabbit / IgG
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu), Mouse (Ms), Rat (Rt)
Immunogen: Synthetic peptide corresponding to a region within amino acids 733 and 796 of VPS35 (Uniprot ID#Q96QK1)
Ordering Information
Pierce VPS35 Antibody
Product #
PA5-21898
Size
100 µL
Price
$324.00
Purchase
Tested Applications Dilution *
Western Blot (WB) 1:500-1:3000
Immunofluorescence (IF) 1:100-1:1000
Immunocytochemistry (ICC) 1:100-1:1000
Immunohistochemistry (Paraffin) (IHC (P)) 1:100-1:1000
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 0.93mg/ml
Purification: Antigen affinity chromatography
Storage Buffer: 0.1M tris, pH 7, with 10% glycerol, 0.1M glycine
Preservative: 0.01% thimerosal
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
PA5-21898 targets VPS35 in ICC, IF, Immunohistochemistry (paraffin) and WB applications and shows reactivity with Human and mouse samples.

The PA5-21898 immunogen is a synthetic peptide corresponding to a region within amino acids 733 and 796 of VPS35 (Uniprot ID#Q96QK1).
General Information
Product Images
  • VPS35 Antibody (PA5-21898) in WB
    PA5-21898_WB_1.jpg

    Western Blot with anti-VPS35 Polyclonal Antibody (PA5-21898) (PA5-21898_WB_1.jpg)

    Western Blot with anti-VPS35 Polyclonal Antibody (PA5-21898)

    Western blot analysis of VPS35 using 50µg of mouse brain lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a VPS35 polyclonal antibody (Product # PA5-21898) at a dilution of 1:1000.
  • VPS35 Antibody (PA5-21898) in WB
    PA5-21898_WB_2.jpg

    Western Blot with anti-VPS35 Polyclonal Antibody (PA5-21898) (PA5-21898_WB_2.jpg)

    Western Blot with anti-VPS35 Polyclonal Antibody (PA5-21898)

    Western blot analysis of VPS35 using 50 µg rat brain lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a VPS35 polyclonal antibody (Product # PA5-21898) at a dilution of 1:500.
  • VPS35 Antibody (PA5-21898) in WB
    PA5-21898_WB_3.jpg

    Western Blot with anti-VPS35 Polyclonal Antibody (PA5-21898) (PA5-21898_WB_3.jpg)

    Western Blot with anti-VPS35 Polyclonal Antibody (PA5-21898)

    Western blot analysis of VPS35 using 30µg of A) H1299 (B) HeLa S3 (C) HepG2 (D) MOLT4 and E) Raji lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a VPS35 polyclonal antibody (Product # PA5-21898) at a dilution of 1:500.
  • VPS35 Antibody (PA5-21898) in IF
    PA5-21898_IF.jpg

    Immunofluorescence with anti-VPS35 Polyclonal Antibody (PA5-21898) (PA5-21898_IF.jpg)

    Immunofluorescence with anti-VPS35 Polyclonal Antibody (PA5-21898)

    Immunofluorescent analysis of VPS35 in methanol-fixed HCT116 cells using a VPS35 polyclonal antibody (Product # PA5-21898) at a 1:500 dilution.
  • VPS35 Antibody (PA5-21898) in IHC (P)
    PA5-21898_IHC.jpg

    Immunohistochemistry (Paraffin) with anti-VPS35 Polyclonal Antibody (PA5-21898) (PA5-21898_IHC.jpg)

    Immunohistochemistry (Paraffin) with anti-VPS35 Polyclonal Antibody (PA5-21898)

    Immunohistochemical analysis of VPS35 in the cytoplasm and membrane of paraffin-embedded colon carcinoma using a VPS35 polyclonal antibody (Product # PA5-21898) at a dilution of 1:250.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific