100 ul of antibody in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 ug/ml BSA and 50% glycerol.
Immunohistochemistry (Paraffin) (IHC (P))
Western Blot (WB)
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
PA5-17460 targets VDAC in IHC (P), and WB applications and shows reactivity with Bovine, Human, Murine, and Rat samples.
The PA5-17460 immunogen is synthetic peptide corresponding to the amino terminus of human VDAC-1.
PA5-17460 detects VDAC which has a predicted molecular weight of approximately 32 kDa.
This gene encodes a voltage-dependent anion channel protein that is a major component of the outer mitochondrial membrane. The encoded protein facilitates the exchange of metabolites and ions across the outer mitochondrial membrane and may regulate mitochondrial functions. This protein also forms channels in the plasma membrane and may be involved in transmembrane electron transport. Alternate splicing results in multiple transcript variants. Multiple pseudogenes of this gene are found on chromosomes 1, 2 3, 6, 9, 12, X and Y.
VDAC Antibody (PA5-17460) in IF
Immunofluorescence with anti-VDAC Polyclonal Antibody (PA5-17460)
PA5-17460 was used in western blot to study the mechanism for the distinct subcellular localization of methionine sulfoxide reductase A isoforms
J Biol Chem. 2010 Jun 4;285(23):18085-94. "Dual sites of protein initiation control the localization and myristoylation of methionine sulfoxide reductase A."
Author(s): Kim G, Cole NB, Lim JC, Zhao H, Levine RL
Number of Citations: 8
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)