VDAC Antibody

VDAC Polyclonal Antibody for Western blot, IHC (P)

>> See 2 other antibodies for VDAC1 (outer mitochondrial membrane protein porin 1; plasmalemmal porin; porin 31HL; porin 31HM; voltage-dependent anion-selective channel protein 1)
Synonyms:
outer mitochondrial membrane protein porin 1; plasmalemmal porin; porin 31HL; porin 31HM; voltage-dependent anion-selective channel protein 1
Details
Host / Isotype: Rabbit
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu), Mouse (Ms), Rat (Rt), Bovine (Bv)
Published Species Reactivity: Mouse (Ms), Rat (Rt)
Immunogen: Synthetic peptide corresponding to the amino terminus of human VDAC-1.
Ordering Information
Pierce VDAC Antibody
Product #
PA5-17460
Size
100 ul
Price
$260.00
Purchase
Add VDAC Antibody to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:1000
Immunohistochemistry (Paraffin) (IHC (P)) 1:75
Published Applications Dilution
Western Blot (WB) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Purification: Affinity chromatography
Storage Buffer: 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol
Preservative: no preservative
Storage Conditions: -20°C
Product Specific Information
PA5-17460 targets VDAC in IHC (P), and WB applications and shows reactivity with Bovine, Human, mouse, and Rat samples.

The PA5-17460 immunogen is synthetic peptide corresponding to the amino terminus of human VDAC-1.

PA5-17460 detects VDAC which has a predicted molecular weight of approximately 32 kDa.
General Information
This gene encodes a voltage-dependent anion channel protein that is a major component of the outer mitochondrial membrane. The encoded protein facilitates the exchange of metabolites and ions across the outer mitochondrial membrane and may regulate mitochondrial functions. This protein also forms channels in the plasma membrane and may be involved in transmembrane electron transport. Alternate splicing results in multiple transcript variants. Multiple pseudogenes of this gene are found on chromosomes 1, 2 3, 6, 9, 12, X and Y.
Product Images
  • VDAC Antibody (PA5-17460) in WB
    PA5-17460_WesternBlot.jpg

    Western Blot with anti-VDAC Polyclonal Antibody (PA5-17460) (PA5-17460_WesternBlot.jpg)

    Western Blot with anti-VDAC Polyclonal Antibody (PA5-17460)

    Western blot analysis of VDAC in extracts from Hela and C2C12 cell lines using VDAC polyclonal antibody (Product # PA5-17460).
  • VDAC Antibody (PA5-17460) in IF
    VDAC_PA5-17460_Antibody_Immunofluorescence(IF)_1A.jpg

    Immunofluorescence with anti-VDAC Polyclonal Antibody (PA5-17460) (VDAC_PA5-17460_Antibody_Immunofluorescence(IF)_1A.jpg)

    Immunofluorescence with anti-VDAC Polyclonal Antibody (PA5-17460)

  • VDAC Antibody (PA5-17460) in IHC
    PA5-17460_Immunohistochemistry_2.jpg

    Immunohistochemistry with anti-VDAC Polyclonal Antibody (PA5-17460) (PA5-17460_Immunohistochemistry_2.jpg)

    Immunohistochemistry with anti-VDAC Polyclonal Antibody (PA5-17460)

    Immunohistochemical analysis of VDAC in paraffin-embedded human colon carcinoma using a VDAC polyclonal antibody (Product # PA5-17460).
Publications:
Western Blot
  Species / Dilution Summary
  Ms / Not Cited
PA5-17460 was used in western blot to study the mechanism for the distinct subcellular localization of methionine sulfoxide reductase A isoforms

J Biol Chem. 2010 Jun 4;285(23):18085-94.
"Dual sites of protein initiation control the localization and myristoylation of methionine sulfoxide reductase A."
Author(s): Kim G, Cole NB, Lim JC, Zhao H, Levine RL
Number of Citations: 8
(See PubMed article )
  Rt / 0
PA5-17460 was used in western blot to investigate age-related changes in synaptic lipid raft proteins

Neurobiol Aging. 2010 Dec;31(12):2146-59.
"Age-associated changes in synaptic lipid raft proteins revealed by two-dimensional fluorescence difference gel electrophoresis."
Author(s): Jiang L, Fang J, Moore DS, Gogichaeva NV, Galeva NA, Michaelis ML, Zaidi A
Number of Citations: 3
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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