Part of Thermo Fisher Scientific

Ubiquitin/Proteasome Fraction II

Ubiquitin/Proteasome Fraction II Extract for Ctrl

Details
Class: Extracts
Type: Extract
Species Reactivity: Not Applicable (N/A)
Ordering Information
Pierce Ubiquitin/Proteasome Fraction II
Product Number Pkg. Size Price Purchase
EP-520 5 mg $311.00
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Storage: -80º C, Avoid Freeze/Thaw Cycles
Form: 5 mg of protein from rabbit reticulocytes in 25 mM HEPES; pH 7.6.


Applications Dilution *
Control (Ctrl) Assay Dependent
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
EP-520 is the protein fraction of cell extract that binds to anion exchange resin. This fraction is essentially ubiquitin-free and ATP-free. EP-520 contains ubiquitin activating enzyme (E1), most ubiquitin conjugating enzymes (E2s), ubiquitin C-terminal hydrolases (UCHs) and the 20S and 26S proteasomes. EP-520 is ideal for demonstrating ubiquitin and/or ATP dependent degradation of in vitro substrates.
General Information
Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signalling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Others are degraded via the vacuolar pathway which includes endosomes, lysosomes, and the endoplasmic reticulum.

The 26S proteasome is an ATP-dependent, multisubunit (~31), barrel-shaped molecular machine with an apparent molecular weight of ~2.5 MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. An enzymatic cascade is responsible for the attachment of multiple ubiquitin molecules to lysine residues of proteins targeted for degradation. Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway. Some examples of affected proteins include those linked to cystic fibrosis, Angelman’s syndrome, and Liddle syndrome.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)