TurboGFP Antibody

TurboGFP Polyclonal Antibody for Western blot, ICC, ELISA

Synonyms:
GFP2, Green Fluoresecent Protein, GFP, Turbo GFP, CopGFP, ppluGFP2,
UniProt ID:
Q6WV12
Details
Host / Isotype: Rabbit / IgG
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Tag (Tag)
Immunogen: Full-length recombinant denatured TurboGFP and non-denatured CopGFP comprising 6XHis Tag
Ordering Information
Pierce TurboGFP Antibody
Product #
PA5-22688
Size
100 µg
Price
$316.00
Purchase
Add TurboGFP Antibody to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:5000 - 1:10000
Immunocytochemistry (ICC) 1:5,000 - 1:10,000
ELISA (ELISA) 1:30,000 - 1:50,000
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Lyophilized
Purification: Antigen affinity chromatography
Storage Buffer: PBS/BBS, pH 7.4, with 0.1% dextran, 0.1% mannitol
Preservative: no preservative
Storage Conditions: -20°C
Product Specific Information
PA5-22688 can be used to recognize denatured and non-denatured TurboGFP and CopGFP in immunocytochemistry, Western blot, and ELISA assays.

TurboGFP is an improved variant of the green fluorescent protein CopGFP cloned from copepod Pontellina plumata (Arthropoda; Crustacea; Maxillopoda; Copepoda)

Reconstitute with sterile water or 50% glycerol to a concentration of 1 mg/ml. Store at -20 C for one year; reconstituted with sterile water, antibody can be stored at +2 - +8°C for three months; reconstituted with 50% glycerol, antibody can be stored at -20°C for six months.
General Information
TurboGFP is an improved variant of the green fluorescent protein CopGFP cloned from copepod Pontellina plumata (Arthropoda; Crustacea; Maxillopoda; Copepoda) [Shagin et al., 2004]. It possesses bright green fluorescence (excitation/ emission max = 482/ 502 nm) that is visible earlier than fluorescence of other green fluorescent proteins.
Product Images
  • TurboGFP Antibody (PA5-22688) in WB
    PA5-22688_TurboGFP_WesternBlot_1.jpg

    Western Blot with anti-TurboGFP Polyclonal Antibody (PA5-22688) (PA5-22688_TurboGFP_WesternBlot_1.jpg)

    Western Blot with anti-TurboGFP Polyclonal Antibody (PA5-22688)

    Western blot analysis of recombinant TurboGFP was performed by loading 50 ng of purified protein onto a 14% SDS-PAGE gel. Recombinant protein was purified from transformed E. coli and the sample was boiled before loading. After transfer, membranes were probed with a rabbit polyclonal antibody recognizing TurboGFP (Product # PA5-22688) at a dilution of 1:20,000. Membranes were probed with a goat anti-rabbit-HRP secondary antibody and then detected by chemiluminescent (ECL) or colorimetric (TMB) detection.
  • TurboGFP Antibody (PA5-22688) in IF
    PA5-22688_TurboGFP_Immunofluorescence_1.jpg

    Immunofluorescence with anti-TurboGFP Polyclonal Antibody (PA5-22688) (PA5-22688_TurboGFP_Immunofluorescence_1.jpg)

    Immunofluorescence with anti-TurboGFP Polyclonal Antibody (PA5-22688)

    Immunofluorescence analysis of TurboGFP expression in HeLa cells shown in brightfield, TurboGFP expression (green), and using a polyclonal antibody recognizing TurboGFP (Product # PA5-22688) at a dilution of 1:3000 followed by an anti-rabbit secondary antibody (red).
  • TurboGFP Antibody (PA5-22688) in IF
    PA5-22688_TurboGFP_Immunofluorescence_2.jpg

    Immunofluorescence with anti-TurboGFP Polyclonal Antibody (PA5-22688) (PA5-22688_TurboGFP_Immunofluorescence_2.jpg)

    Immunofluorescence with anti-TurboGFP Polyclonal Antibody (PA5-22688)

    Immunofluorescence analysis of TurboGFP expression in HeLa cells shown in brightfield, TurboGFP expression (green), and using a polyclonal antibody recognizing TurboGFP (Product # PA5-22688) at a dilution of 1:3000 followed by an anti-rabbit secondary antibody (red).
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific