TurboGFP Antibody
TurboGFP Polyclonal Antibody for ELISA, ICC, IHC (P), Western blot
Synonyms:
GFP2, Green Fluoresecent Protein, GFP, Turbo GFP, CopGFP, ppluGFP2,
UniProt ID:
Q6WV12
| Details | |
| Host / Isotype: | Rabbit / IgG |
| Class: | Polyclonal |
| Type: | Antibody |
| Species Reactivity: | Tag (Tag) |
| Immunogen: | Full-length recombinant denatured TurboGFP and non-denatured CopGFP comprising 6XHis Tag |
| Ordering Information | ||||
| Pierce TurboGFP Antibody |
| Storage: | -20°C |
| Form: | 100 µg of lyophilized antibody from the buffer containing 0.1M NaCl, 0.01M Na2HPO4, 0.01M NaBO4; pH 7.4, with 0.1% mannitol and 0.1% dextran. |
| Applications | Dilution * |
| Western Blot (WB) | 1:5000 - 1:10000 |
| ELISA (ELISA) | 1:30,000 - 1:50,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay Dependent |
| Immunocytochemistry (ICC) | 1:5,000 - 1:10,000 |
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* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
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| Product Specific Information |
| PA5-22688 can be used to recognize denatured and non-denatured TurboGFP and CopGFP, in immunocytochemistry, immunohistochemistry (paraffin), western blot, and ELISA assays. TurboGFP is an improved variant of the green fluorescent protein CopGFP cloned from copepod Pontellina plumata (Arthropoda; Crustacea; Maxillopoda; Copepoda) Reconstitute with sterile water or 50% glycerol to a concentration of 1 mg/ml. Store at -20 C for one year; reconstituted with sterile water, antibody can be stored at +2 - +8°C for three months; reconstituted with 50% glycerol, antibody can be stored at -20°C for six months. |
| General Information |
| TurboGFP is an improved variant of the green fluorescent protein CopGFP cloned from copepod Pontellina plumata (Arthropoda; Crustacea; Maxillopoda; Copepoda) [Shagin et al., 2004]. It possesses bright green fluorescence (excitation/ emission max = 482/ 502 nm) that is visible earlier than fluorescence of other green fluorescent proteins. |
| Product Images |
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TurboGFP Antibody (PA5-22688) in IF
Immunofluorescence with anti-TurboGFP Polyclonal Antibody (PA5-22688)
Immunofluorescence analysis of TurboGFP expression in HeLa cells shown in brightfield, TurboGFP expression (green), and using a polyclonal antibody recognizing TurboGFP (Product # PA5-22688) at a dilution of 1:3000 followed by an anti-rabbit secondary antibody (red). -
TurboGFP Antibody (PA5-22688) in IF
Immunofluorescence with anti-TurboGFP Polyclonal Antibody (PA5-22688)
Immunofluorescence analysis of TurboGFP expression in HeLa cells shown in brightfield, TurboGFP expression (green), and using a polyclonal antibody recognizing TurboGFP (Product # PA5-22688) at a dilution of 1:3000 followed by an anti-rabbit secondary antibody (red). -
TurboGFP Antibody (PA5-22688) in WB
Western Blot with anti-TurboGFP Polyclonal Antibody (PA5-22688)
Western blot analysis of recombinant TurboGFP was performed by loading 50 ng of purified protein onto a 14% SDS-PAGE gel. Recombinant protein was purified from transformed E. coli and the sample was boiled before loading. After transfer, membranes were probed with a rabbit polyclonal antibody recognizing TurboGFP (Product # PA5-22688) at a dilution of 1:20,000. Membranes were probed with a goat anti-rabbit-HRP secondary antibody and then detected by chemiluminescent (ECL) or colorimetric (TMB) detection.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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TurboGFP Antibodies > TurboGFP Polyclonal Antibody PA5-22688
