500 ul of antibody purified from the ascites fluid by Protein G chromatography (200 ug/ml). Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.
Applications
Dilution *
Immunofluorescence (IF)
Assay Dependent
Immunohistochemistry (Paraffin) (IHC (P))
1-2 µg/ml
Western Blot (WB)
0.5-1.0 µg/ml
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
MA5-11732 targets Tubulin Beta in IF, IHC (P), and WB applications and shows reactivity with Amphibian, Bovine, Chicken, Human, Insect, Murine, Plant, Rabbit, and Rat samples.
The MA5-11732 immunogen is purified tubulin.
General Information
Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tubulin, which is a dimer of two 55kDa subunits: alpha and Beta. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton.
Product Images
Filter :
IHC using MA5-11732
Immunohistochemistry with anti-Tubulin Beta Monoclonal Antibody [TBN06 (Tub 2.5)] (MA5-11732)
Formalin-fixed, paraffin-embedded human lung stained with Tubulin beta antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic and surface staining of ciliated epithelial cells in bronchiole.
Formalin-fixed, paraffin-embedded human lung stained with Tubulin beta antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic and surface staining of ciliated epithelial cells in bronchiole.
WB using MA5-11732
Western Blot with anti-Tubulin Beta Monoclonal Antibody [TBN06 (Tub 2.5)] (MA5-11732)