Troponin T Cardiac Isoform Antibody (13-11)

Troponin T Cardiac Isoform Monoclonal Antibody for IF, ICC, IHC (P), IM

>> See 22 other antibodies for TNNT2 (cardiac muscle troponin T, cardiomyopathy, dilated 1D (autosomal dominant), cardiomyopathy, hypertrophic 2, troponin T, cardiac muscle, troponin T2, cardiac)
Synonyms:
cardiac muscle troponin T, cardiomyopathy, dilated 1D (autosomal dominant), cardiomyopathy, hypertrophic 2, troponin T, cardiac muscle, troponin T2, cardiac
Details
Host / Isotype: Mouse / IgG1
Class: Monoclonal
Type: Antibody
Clone: 13-11
Tested Species Reactivity: Human (Hu), Mouse (Ms), Rat (Rt), Avian (Av), Canine (Ca), Chicken (Ck), Fish (Fs), Guinea Pig (GP), Porcine (Po), Rabbit (Rb)
Published Species Reactivity: Canine (Ca), Human (Hu), Mouse (Ms), Rabbit (Rb), Rat (Rt)
Immunogen: Purified rabbit cardiac troponin T isoform (TnT4R)
Ordering Information
Pierce Troponin T Cardiac Isoform Antibody (13-11)
Product #
MA5-12960
Size
500 ul
Price
$299.00
Purchase
Add Troponin T Cardiac Isoform Antibody (13-11) to your cart.
Tested Applications Dilution *
Immunofluorescence (IF) 1:100-1:500
Immunocytochemistry (ICC) 1:100-1:500
Immunohistochemistry (Paraffin) (IHC (P)) 1-2 µg/ml
Immunomicroscopy (IM) Assay Dependent
Published Applications Dilution
Western Blot (WB) See publications below
Immunocytochemistry (ICC) See publications below
Immunohistochemistry (IHC) See publications below
Flow Cytometry (FACS) See publications below
ELISA (ELISA) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 0.2mg/ml
Purification: Protein G
Storage Buffer: PBS, pH 7.4, with 0.2% BSA
Preservative: 0.09% sodium azide
Storage Conditions: 4° C
Product Specific Information
MA5-12960 targets Troponin T Cardiac Isoform in IF/ICC, IHC (P), and IM applications and shows reactivity with Avian, Canine, Chicken, Fish, Guinea Pig, Human, mouse, Porcine, Rabbit, and Rat samples.

The MA5-12960 immunogen is purified rabbit cardiac troponin T isoform (TnT4R).
General Information
Troponin T is a thin filament regulatory protein of striated muscle that binds to troponin C and I and to tropomyosin and is required for calcium-dependent ATPase activity of myofibrillar proteins. It is important in proper muscle function and myofibril formation. In the human heart, four cTnT isoforms are expressed in a developmentally regulated manner. This expression in the human heart is affected by heart failure.
Product Images
  • Troponin T Cardiac Isoform Antibody (MA5-12960) in IF
    Troponin-T-Cardiac-Isoform_Antibody_MA5-12960_Immunofluorescence_1_20140428082114.jpg

    Immunofluorescence with anti-Troponin T Cardiac Isoform Monoclonal Antibody [13-11] (MA5-12960) (Troponin-T-Cardiac-Isoform_Antibody_MA5-12960_Immunofluorescence_1_20140428082114.jpg)

    Immunofluorescence with anti-Troponin T Cardiac Isoform Monoclonal Antibody [13-11] (MA5-12960)

    Immunofluorescent analysis of Troponin T Cardiac Isoform (green) in cultured primary cardiomyocytes. Primary cardiomyocytes were isolated and cultured using the Primary Cardiomyocyte Isolation Kit (Product # 88281). Cells were grown in 24-well plates (seeded at 5x10^5 cells/well) or in 35mm glass bottom plates (at a density of 2.5 x 106 cells/well). At day 1 and day 7, cells were fixed with 4% paraformaldehyde, permeablilized with 0.1% Triton X-100 in HBSS for 10 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were probed with a Troponin T Cardiac Isoform monoclonal antibody (Product # MA5-12960) at dilution of 1:300 for 2 hours at room temperature or overnight at 4 ºC, washed with HBSS, and incubated with a DyLight 488-conjugated goat anti-mouse IgG secondary antibody (Product # 35502) at dilution of 1:500 for 1 hour at room temperature. Nuclei (blue) were visualized using Hoechst 33342 (Product # 62249) and dead cells were visualized using Propidium iodide (red). Images were taken at 20X (Panels A and B) or 60X (Panel C) magnifications on a Carl Zeiss microscope (AxioVision Rel. 4.7).
  • Troponin T Cardiac Isoform Antibody (MA5-12960) in IHC
    MA5-12960_Immunohistochemistry.jpg

    Immunohistochemistry with anti-Troponin T Cardiac Isoform Monoclonal Antibody [13-11] (MA5-12960) (MA5-12960_Immunohistochemistry.jpg)

    Immunohistochemistry with anti-Troponin T Cardiac Isoform Monoclonal Antibody [13-11] (MA5-12960)

    Formalin-fixed, paraffin-embedded human heart stained with Troponin antibody using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining of cardiac muscle cells.
  • Troponin T Cardiac Isoform Antibody (MA5-12960) in IHC
    MA5-12960_Immunohistochemistry2.jpg

    Immunohistochemistry with anti-Troponin T Cardiac Isoform Monoclonal Antibody [13-11] (MA5-12960) (MA5-12960_Immunohistochemistry2.jpg)

    Immunohistochemistry with anti-Troponin T Cardiac Isoform Monoclonal Antibody [13-11] (MA5-12960)

    Formalin-fixed, paraffin-embedded rat heart stained with Troponin T antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of cardiac muscles.
Publications:
Western Blot
  Species / Dilution Summary
  Hu / Not Cited
MA5-12960 was used in western blot to investigate the effect of electrostimulation on fibroblast pre-commitment into cardiomyocytes

Biochem Biophys Res Commun. 2008 Jun 6;370(3):450-5.
"Electrostimulation induces cardiomyocyte predifferentiation of fibroblasts."
Author(s): Genovese JA, Spadaccio C, Langer J, Habe J, Jackson J, Patel AN
Number of Citations: 2
(See PubMed article )
Immunocytochemistry
  Species / Dilution Summary
  Hu / 1:300
MA5-12960 was used in immunocytochemistry to examine the role of gremlin in cardiomyogenesis

PLoS One. 2008 Jun 11;3(6):e2407.
"Gremlin enhances the determined path to cardiomyogenesis."
Author(s): Kami D, Shiojima I, Makino H, Matsumoto K, Takahashi Y, Ishii R, Naito AT, Toyoda M, Saito H, Watanabe M, Komuro I, Umezawa A
Number of Citations: 2
(See PubMed article )
  Hu / 1:100
MA5-12960 was used in immunocytochemistry to develop a new method for evaluating drug-induced proarrhythmic risk

Toxicol Sci. 2011 Sep;123(1):281-9.
"Estimating the risk of drug-induced proarrhythmia using human induced pluripotent stem cell-derived cardiomyocytes."
Author(s): Guo L, Abrams RM, Babiarz JE, Cohen JD, Kameoka S, Sanders MJ, Chiao E, Kolaja KL
Number of Citations: 1
(See PubMed article )
  Ms / 1:200
MA5-12960 was used in immunocytochemistry to investigate the characteristics of embryonic stem cells derived from inner cell mass

Biochem Biophys Res Commun. 2005 Jun 17;331(4):1577-86.
"Single inner cell masses yield embryonic stem cell lines differing in lifr expression and their developmental potential."
Author(s): Lauss M, Stary M, Tischler J, Egger G, Puz S, Bader-Allmer A, Seiser C, Weitzer G
Number of Citations: 1
(See PubMed article )
  Ms / 1:100
MA5-12960 was used in immunocytochemistry to examine the totipotency of mouse epiblast

Nature. 2007 Jul 12;448(7150):196-9.
"New cell lines from mouse epiblast share defining features with human embryonic stem cells."
Author(s): Tesar PJ, Chenoweth JG, Brook FA, Davies TJ, Evans EP, Mack DL, Gardner RL, McKay RD
Number of Citations: 195
(See PubMed article )
  Ms / 1:100
MA5-12960 was used in immunocytochemistry to examine the cardiogenic differentiation of embryonic stem cells in vitro

J Biosci Bioeng. 2007 May;103(5):412-9.
"Coexpression of platelet-derived growth factor receptor alpha and fetal liver kinase 1 enhances cardiogenic potential in embryonic stem cell differentiation in vitro."
Author(s): Hirata H, Kawamata S, Murakami Y, Inoue K, Nagahashi A, Tosaka M, Yoshimura N, Miyamoto Y, Iwasaki H, Asahara T, Sawa Y
Number of Citations: 2
(See PubMed article )
  Ms / 2 ug/ml
MA5-12960 was used in immunocytochemistry to examine the myocardic differentiation of induced pluripotent murine stem cells

Circulation. 2008 Jul 29;118(5):507-17.
"Generation of functional murine cardiac myocytes from induced pluripotent stem cells."
Author(s): Mauritz C, Schwanke K, Reppel M, Neef S, Katsirntaki K, Maier LS, Nguemo F, Menke S, Haustein M, Hescheler J, Hasenfuss G, Martin U
Number of Citations: 54
(See PubMed article )
  Ms / Not Cited
MA5-12960 was used in flow cytometry and immunocytochemistry to investigate the influence of suramin on mouse embryonic stem cell differentiation into sinus node-like cells

Int J Cardiol. 2011 Feb 17;147(1):95-111.
"Differentiation induction of mouse embryonic stem cells into sinus node-like cells by suramin."
Author(s): Wiese C, Nikolova T, Zahanich I, Sulzbacher S, Fuchs J, Yamanaka S, Graf E, Ravens U, Boheler KR, Wobus AM
Number of Citations: 0
(See PubMed article )
  Ms / 1:200
MA5-12960 was used in immunocytochemistry to study role of mesenchymal cells in cardiac differential and calcium homeostasis

J Mol Cell Cardiol. 2010 Apr;48(4):735-45.
"Delayed enrichment of mesenchymal cells promotes cardiac lineage and calcium transient development."
Author(s): Grajales L, García J, Banach K, Geenen DL
Number of Citations: 1
(See PubMed article )
  Ms / 1:100
MA5-12960 was used in immunocytochemistry to isolate and characterize Acta2-positive cardiomyocytes

Cell Physiol Biochem. 2010;25(6):595-604.
"Isolation and functional characterization of alpha-smooth muscle actin expressing cardiomyocytes from embryonic stem cells."
Author(s): Potta SP, Liang H, Winkler J, Doss MX, Chen S, Wagh V, Pfannkuche K, Hescheler J, Sachinidis A
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
MA5-12960 was used in immunocytochemistry to investigate the differentiation of mouse fat-derived stem cells into heart cells

Mol Cell Biochem. 2010 Nov;344(1-2):1-9.
"Icariin-mediated differentiation of mouse adipose-derived stem cells into cardiomyocytes."
Author(s): Jin MS, Shi S, Zhang Y, Yan Y, Sun XD, Liu W, Liu HW
Number of Citations: 0
(See PubMed article )
  Ms / 1:100
MA5-12960 was used in immunocytochemistry to investigate the effect of triiodothyronine on cardiac differentiation and maturation of embryonic stem cells

Mol Endocrinol. 2010 Sep;24(9):1728-36.
"Triiodothyronine promotes cardiac differentiation and maturation of embryonic stem cells via the classical genomic pathway."
Author(s): Lee YK, Ng KM, Chan YC, Lai WH, Au KW, Ho CY, Wong LY, Lau CP, Tse HF, Siu CW
Number of Citations: 5
(See PubMed article )
  Ms / 1:100
MA5-12960 was used in immunocytochemistry to investigate the effect of ouabain on cardiac differentiation

Acta Pharmacol Sin. 2011 Jan;32(1):52-61.
"Ouabain facilitates cardiac differentiation of mouse embryonic stem cells through ERK1/2 pathway."
Author(s): Lee YK, Ng KM, Lai WH, Man C, Lieu DK, Lau CP, Tse HF, Siu CW
Number of Citations: 1
(See PubMed article )
  Ms / 1:200
MA5-12960 was used in immunocytochemistry to study the effects of dioxin on the differentiation of mouse embryonic stem cells into cardiomyocytes

Toxicol Lett. 2011 May 10;202(3):226-36.
"The differentiation of cardiomyocytes from mouse embryonic stem cells is altered by dioxin."
Author(s): Neri T, Merico V, Fiordaliso F, Salio M, Rebuzzini P, Sacchi L, Bellazzi R, Redi CA, Zuccotti M, Garagna S
Number of Citations: 0
(See PubMed article )
  Rt / 1:200
MA5-12960 was used in immunocytochemistry to invesigate the therapeutic effect of bone marrow stromal cell transplantation on acute myocardial infarction

Cytotherapy. 2012 May;14(5):563-9.
"Bone marrow stromal cell transplantation combined with angiotensin-converting enzyme inhibitor treatment in rat with acute myocardial infarction and the role of insulin-like growth factor-1."
Author(s): Huang YL, Kuang J, Hu YZ, Song YB, Qiu RF, Mai WY
Number of Citations: 0
(See PubMed article )
Immunohistochemistry
  Species / Dilution Summary
  Ca / Not Cited
MA5-12960 was used in immunohistochemistry to study the therapeutic effects of treating canine ischemic cardiomyopathy with autologous cardioplasty using skeletal myoblasts plus bone marrow cells

J Thorac Cardiovasc Surg. 2005 Sep;130(3):646-53.
"Combined autologous cellular cardiomyoplasty with skeletal myoblasts and bone marrow cells in canine hearts for ischemic cardiomyopathy."
Author(s): Memon IA, Sawa Y, Miyagawa S, Taketani S, Matsuda H
Number of Citations: 3
(See PubMed article )
  Hu / Not Cited
MA5-12960 was used in immunohistochemistry to investigate cardiac differentiation of human embryonic stem cells induced by cobalt chloride

Cell Reprogram. 2011 Dec;13(6):527-37.
"Cobalt chloride pretreatment promotes cardiac differentiation of human embryonic stem cells under atmospheric oxygen level."
Author(s): Ng KM, Chan YC, Lee YK, Lai WH, Au KW, Fung ML, Siu CW, Li RA, Tse HF
Number of Citations: 0
(See PubMed article )
  Hu / 0
MA5-12960 was used in immunohistochemistry to study the role of integrin-linked kinase in cardiomyogenesis

PLoS One. 2012;7(5):e37802.
"ILK induces cardiomyogenesis in the human heart."
Author(s): Traister A, Aafaqi S, Masse S, Dai X, Li M, Hinek A, Nanthakumar K, Hannigan G, Coles JG
Number of Citations: 0
(See PubMed article )
  Ms / 1:500
MA5-12960 was used in immunohistochemistry to study the role of fetal liver-derived stem cells in regenerative healing in mouse heart

Cloning Stem Cells. 2004;6(4):352-63.
"The MRL mouse heart healing response shows donor dominance in allogeneic fetal liver chimeric mice."
Author(s): Bedelbaeva K, Gourevitch D, Clark L, Chen P, Leferovich JM, Heber-Katz E
Number of Citations: 2
(See PubMed article )
  Ms / Not Cited
MA5-12960 was used in immunohistochemistry to investigate the electrophysiological effects of granulocyte colony-stimulating factor/stem cell factor on infarcted hearts

J Exp Med. 2006 Jan 23;203(1):87-97.
"G-CSF/SCF reduces inducible arrhythmias in the infarcted heart potentially via increased connexin43 expression and arteriogenesis."
Author(s): Kuhlmann MT, Kirchhof P, Klocke R, Hasib L, Stypmann J, Fabritz L, Stelljes M, Tian W, Zwiener M, Mueller M, Kienast J, Breithardt G, Nikol S
Number of Citations: 11
(See PubMed article )
  Ms / Not Cited
MA5-12960 was used in immunohistochemistry to investigate the effect of brown adipose tissue-derived cells on cardiomyocyte differentiation

Stem Cells. 2007 May;25(5):1326-33.
"Cardiac stem cells in brown adipose tissue express CD133 and induce bone marrow nonhematopoietic cells to differentiate into cardiomyocytes."
Author(s): Yamada Y, Yokoyama S, Wang XD, Fukuda N, Takakura N
Number of Citations: 1
(See PubMed article )
  Ms / 1:100
MA5-12960 was used in immunohistochemistry to evaluate ALCAM as a surface marker for early murine cardiomyocytes

Cells Tissues Organs. 2006;184(3-4):172-80.
"ALCAM (CD166) is a surface marker for early murine cardiomyocytes."
Author(s): Hirata H, Murakami Y, Miyamoto Y, Tosaka M, Inoue K, Nagahashi A, Jakt LM, Asahara T, Iwata H, Sawa Y, Kawamata S
Number of Citations: 1
(See PubMed article )
  Ms / 1:100
MA5-12960 was used in immunohistochemistry to evaluate superparamagnetic iron oxide nanoparticle labeling of embryonic stem cells during myocardial transplant and differentiation

Biochem Biophys Res Commun. 2009 Feb 20;379(4):898-903.
"Effects of iron oxide nanoparticles on cardiac differentiation of embryonic stem cells."
Author(s): Au KW, Liao SY, Lee YK, Lai WH, Ng KM, Chan YC, Yip MC, Ho CY, Wu EX, Li RA, Siu CW, Tse HF
Number of Citations: 7
(See PubMed article )
  Ms / 1:200
MA5-12960 was used in immunohistochemistry to study the developmental origin of cardiac and smooth muscle cells in the cardiac inflow tract

J Mol Cell Cardiol. 2011 Feb;50(2):337-45.
"Cardiac origin of smooth muscle cells in the inflow tract."
Author(s): Nakano H, Williams E, Hoshijima M, Sasaki M, Minamisawa S, Chien KR, Nakano A
Number of Citations: 0
(See PubMed article )
  Ms / Not Cited
MA5-12960 was used in immunohistochemistry to investigate the effect of cardiogenol C on the differentiation of mouse hair bulge progenitor cells into cardiomyocyte-like cells

Proteome Sci. 2011 Jan 19;9(1):3.
"Cardiogenol C can induce Mouse Hair Bulge Progenitor Cells to Transdifferentiate into Cardiomyocyte-like Cells."
Author(s): Yau WW, Tang MK, Chen E, Yaoyao, Wong IW, Lee HS, Lee KKh
Number of Citations: 0
(See PubMed article )
  Ms / 1:100
MA5-12960 was used in immunohistochemistry to develop a ROSA26-iPSC mouse strain as a model for studying cellular differentiation and dedifferentiation

Cell Rep. 2013 Feb 21;3(2):335-41.
"The ROSA26-iPSC mouse: a conditional, inducible, and exchangeable resource for studying cellular (De)differentiation."
Author(s): Haenebalcke L, Goossens S, Dierickx P, Bartunkova S, D'Hont J, Haigh K, Hochepied T, Wirth D, Nagy A, Haigh JJ
Number of Citations: 1
(See PubMed article )
Flow Cytometry
  Species / Dilution Summary
  Hu / 0
MA5-12960 was used in flow cytometry to characterize cell surface markers for human cardiomyocytes derived from human embryonic and induced pluripotent stem cells

PLoS One. 2011;6(8):e23657.
"Efficient and scalable purification of cardiomyocytes from human embryonic and induced pluripotent stem cells by VCAM1 surface expression."
Author(s): Uosaki H, Fukushima H, Takeuchi A, Matsuoka S, Nakatsuji N, Yamanaka S, Yamashita JK
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
MA5-12960 was used in flow cytometry and immunocytochemistry to investigate the influence of suramin on mouse embryonic stem cell differentiation into sinus node-like cells

Int J Cardiol. 2011 Feb 17;147(1):95-111.
"Differentiation induction of mouse embryonic stem cells into sinus node-like cells by suramin."
Author(s): Wiese C, Nikolova T, Zahanich I, Sulzbacher S, Fuchs J, Yamanaka S, Graf E, Ravens U, Boheler KR, Wobus AM
Number of Citations: 0
(See PubMed article )
ELISA
  Species / Dilution Summary
  Ms / 0.25 ug/mL
Rb / 0.25 ug/mL
MA5-12960 was used in ELISA and ELISA to investigate the number and size of myocytes expressing beta-myosin heavy chain after transverse aortic constriction

Circ Res. 2011 Sep 2;109(6):629-38.
"?-myosin heavy chain is induced by pressure overload in a minor subpopulation of smaller mouse cardiac myocytes."
Author(s): López JE, Myagmar BE, Swigart PM, Montgomery MD, Haynam S, Bigos M, Rodrigo MC, Simpson PC
Number of Citations: 1
(See PubMed article )
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