Thymidine Phosphorylase Antibody (P-GF.44C)

Thymidine Phosphorylase Monoclonal Antibody for IHC (P)

>> See 5 other antibodies for TYMP (gliostatin, tdRPase)
Synonyms:
gliostatin, tdRPase
Entrez Gene ID:
Details
Host / Isotype: Mouse / IgG1
Class: Monoclonal
Type: Antibody
Clone: P-GF.44C
Tested Species Reactivity: Human (Hu), Mouse (Ms), Rat (Rt)
Published Species Reactivity: Human (Hu)
Immunogen: Recombinant full length human thymidine phosphorylase (TP/PD-ECGF) protein
Ordering Information
Pierce Thymidine Phosphorylase Antibody (P-GF.44C)
Product #
MA5-13542
Size
500 ul
Price
$316.00
Purchase
Add Thymidine Phosphorylase Antibody (P-GF.44C) to your cart.
Tested Applications Dilution *
Immunohistochemistry (Paraffin) (IHC (P)) 1-2 µg/ml
Published Applications Dilution
Western Blot (WB) See publications below
Immunohistochemistry (IHC) See publications below
ELISA (ELISA) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 0.2mg/ml
Purification: Protein G
Storage Buffer: PBS, pH 7.4, with 0.2% BSA
Preservative: 0.09% sodium azide
Storage Conditions: 4° C, do not freeze
Product Specific Information
MA5-13542 targets Thymidine Phosphorylase in IHC (P) applications and shows reactivity with Human, mouse, and Rat samples.

The MA5-13542 immunogen is recombinant full length human thymidine phosphorylase (TP/PD-ECGF) protein.
General Information
Platelet-derived endothelial growth factor (PD-ECGF), same as thymidine phosphorylase (TP) or gliostatin. In the presence of inorganic orthophosphate, it catalyses the reversible phospholytic cleavage of thymidine and deoxyuridine to their corresponding bases and 2-deoxyribose-1-phosphate. It is both chemotactic and mitogenic for endothelial cells and a non-heparin binding angiogenic factor present in platelets. It is also involved in transformation of fluoropyrimidines, cytotoxic agents used in the treatment of a variety of malignancies, into active cytotoxic metabolites.
Product Images
  • Thymidine Phosphorylase Antibody (MA5-13542) in IHC
    MA5-13542_Immunohistochemistry.jpg

    Immunohistochemistry with anti-Thymidine Phosphorylase Monoclonal Antibody [P-GF.44C] (MA5-13542) (MA5-13542_Immunohistochemistry.jpg)

    Immunohistochemistry with anti-Thymidine Phosphorylase Monoclonal Antibody [P-GF.44C] (MA5-13542)

    Formalin-fixed, paraffin-embedded human breast carcinoma stained with Thymidine Phosphorylase antibody using peroxidase-conjugate and AEC chromogen. Note nuclear and cytoplasmic staining of tumor cells.
Publications:
Western Blot
  Species / Dilution Summary
  Hu / 1:500
MA5-13542 was used in immunohistochemistry and western blot to study the interferon signaling pathway in oral cancer

Mol Cell Proteomics. 2009 Jul;8(7):1453-74.
"Enhanced interferon signaling pathway in oral cancer revealed by quantitative proteome analysis of microdissected specimens using 16O/18O labeling and integrated two-dimensional LC-ESI-MALDI tandem MS."
Author(s): Chi LM, Lee CW, Chang KP, Hao SP, Lee HM, Liang Y, Hsueh C, Yu CJ, Lee IN, Chang YJ, Lee SY, Yeh YM, Chang YS, Chien KY, Yu JS
Number of Citations: 1
(See PubMed article )
Immunohistochemistry
  Species / Dilution Summary
  Hu / 1:200
MA5-13542 was used in immunohistochemistry to investigate the mechanism for the increased vascularity in preinvasive breast disease

Clin Cancer Res. 1999 Oct;5(10):2867-76.
"Angiogenic growth factors in preinvasive breast disease."
Author(s): Heffelfinger SC, Miller MA, Yassin R, Gear R
Number of Citations: 14
(See PubMed article )
  Hu / 1:100
MA5-13542 was used in immunohistochemistry to study Helicobacter pylori antigen and angiogenesis factors in human gastric carcinoma

World J Gastroenterol. 2006 Sep 14;12(34):5465-72.
"H pylori status and angiogenesis factors in human gastric carcinoma."
Author(s): Mangia A, Chiriatti A, Ranieri G, Abbate I, Coviello M, Simone G, Zito FA, Montemurro S, Rucci A, Di Leo A, Tommasi S, Berloco P, Xu JM, Paradiso A
Number of Citations: 1
(See PubMed article )
  Hu / 1:100
MA5-13542 was used in immunohistochemistry to study the role of thymidine phosphorylase expression in increased sensitivity to 5-fluorouracil in oropharyngeal cancer patients

J Cell Mol Med. 2007 Mar-Apr;11(2):362-8.
"A possible role of thymidine phosphorylase expression and 5-fluorouracil increased sensitivity in oropharyngeal cancer patients."
Author(s): Ranieri G, Grammatica L, Patruno R, Zito AF, Valerio P, Iacobellis S, Gadaleta C, Gasparini G, Ribatti D
Number of Citations: 0
(See PubMed article )
  Hu / 1:200
MA5-13542 was used in immunohistochemistry to investigate the association between neoadjuvants docetaxel and capecitabine and prostate cancer

J Urol. 2008 Mar;179(3):911-5; discussion 915-6.
"Neoadjuvant docetaxel and capecitabine in patients with high risk prostate cancer."
Author(s): Friedman J, Dunn RL, Wood D, Vaishampayan U, Wu A, Bradley D, Montie J, Sarkar FH, Shah RB, Hussain M
Number of Citations: 1
(See PubMed article )
  Hu / 1:40
MA5-13542 was used in immunohistochemistry to study the efficacy of combined cetuximab and modified FOLFOX6 treatment in a phase II gastric cancer trial

Br J Cancer. 2009 Jan 27;100(2):298-304.
"Phase II study and biomarker analysis of cetuximab combined with modified FOLFOX6 in advanced gastric cancer."
Author(s): Han SW, Oh DY, Im SA, Park SR, Lee KW, Song HS, Lee NS, Lee KH, Choi IS, Lee MH, Kim MA, Kim WH, Bang YJ, Kim TY
Number of Citations: 15
(See PubMed article )
  Hu / 1:500
MA5-13542 was used in immunohistochemistry and western blot to study the interferon signaling pathway in oral cancer

Mol Cell Proteomics. 2009 Jul;8(7):1453-74.
"Enhanced interferon signaling pathway in oral cancer revealed by quantitative proteome analysis of microdissected specimens using 16O/18O labeling and integrated two-dimensional LC-ESI-MALDI tandem MS."
Author(s): Chi LM, Lee CW, Chang KP, Hao SP, Lee HM, Liang Y, Hsueh C, Yu CJ, Lee IN, Chang YJ, Lee SY, Yeh YM, Chang YS, Chien KY, Yu JS
Number of Citations: 1
(See PubMed article )
  Hu / 1:5000
MA5-13542 was used in immunohistochemistry to identify the prognostic markers for advanced colorectal cancer chemotherapy

Eur J Cancer. 2009 Jul;45(11):1999-2006.
"Predictive and prognostic markers for the outcome of chemotherapy in advanced colorectal cancer, a retrospective analysis of the phase III randomised CAIRO study."
Author(s): Koopman M, Venderbosch S, van Tinteren H, Ligtenberg MJ, Nagtegaal I, Van Krieken JH, Punt CJ
Number of Citations: 5
(See PubMed article )
  Hu / 1:4000
MA5-13542 was used in immunohistochemistry to investigate the expression level and role of thymidine kinase 1 and thymidine phosphorylase in non-small-cell lung carcinoma

J Histochem Cytochem. 2009 Nov;57(11):1087-97.
"Thymidine kinase 1 and thymidine phosphorylase expression in non-small-cell lung carcinoma in relation to angiogenesis and proliferation."
Author(s): Brockenbrough JS, Morihara JK, Hawes SE, Stern JE, Rasey JS, Wiens LW, Feng Q, Vesselle H
Number of Citations: 1
(See PubMed article )
  Hu / 1:300
MA5-13542 was used in immunohistochemistry to investigate the mechanism for the induction of thymidine phosphorylase by Epstein-Barr virus latent membrane protein 1

Cell Signal. 2010 Jul;22(7):1132-42.
"Epstein-Barr virus latent membrane protein 1 induces the chemotherapeutic target, thymidine phosphorylase, via NF-kappaB and p38 MAPK pathways."
Author(s): Chen CC, Chen LC, Liang Y, Tsang NM, Chang YS
Number of Citations: 3
(See PubMed article )
  Hu / 1:100
MA5-13542 was used in immunohistochemistry to study the efficacy and toxicity of combination chemotherapy in head and neck cancer.

Ann Oncol. 2011 Feb;22(2):417-23.
"A phase II study of combination chemotherapy with capecitabine and cisplatin in patients with metastatic or recurrent squamous cell carcinoma of the head and neck."
Author(s): Won YW, Park YH, Ahn MJ, Do IG, Ko YH, Park K
Number of Citations: 0
(See PubMed article )
  Hu / 1:100
MA5-13542 was used in immunohistochemistry to study biomarkers for predicting the response to capecitabine and cisplatin combination chemotherapy in metastatic oesophageal squamous cell cancer

Br J Cancer. 2010 Sep 7;103(6):845-51.
"Thymidine synthase, thymidine phosphorylase, and excision repair cross-complementation group 1 expression as predictive markers of capecitabine plus cisplatin chemotherapy as first-line treatment for patients with advanced oesophageal squamous cell carcinoma."
Author(s): Lee S, Park YH, Kim KH, Cho EY, Ahn YC, Kim K, Shim YM, Ahn JS, Park K, Im YH
Number of Citations: 2
(See PubMed article )
  Hu / 1:500
MA5-13542 was used in immunohistochemistry to study combination chemotherapy with S-1 and platinum in advanced hepatocellular carcinoma.

Anticancer Res. 2010 Dec;30(12):5245-50.
"Combination chemotherapy with S-1 and platinum in advanced hepatocellular carcinoma."
Author(s): Kim SJ, Han SW, Oh DY, Yi NJ, Kim YJ, Im SA, Yoon JH, Kang GH, Suh KS, Bang YJ, Jang JJ, Kim TY
Number of Citations: 0
(See PubMed article )
ELISA
  Species / Dilution Summary
  Hu / Not Cited
MA5-13542 was used in ELISA to investigate the importance of circulating angiogenic factors in dendritic cell-based cancer immunotherapy

Cancer. 2003 Nov 15;98(10):2291-301.
"Monitoring of circulating angiogenic factors in dendritic cell-based cancer immunotherapy."
Author(s): Brostjan C, Bayer A, Zommer A, Gornikiewicz A, Roka S, Benkö T, Yaghubian R, Jakesz R, Steger G, Gnant M, Friedl J, Stift A
Number of Citations: 1
(See PubMed article )
  Hu / 500 ng/ml
MA5-13542 was used in ELISA to investigate the systemic angiogenic status in patients with colorectal cancer undergoing bevacizumab therapy

Clin Cancer Res. 2008 Apr 1;14(7):2065-74.
"Neoadjuvant treatment of colorectal cancer with bevacizumab: the perioperative angiogenic balance is sensitive to systemic thrombospondin-1 levels."
Author(s): Brostjan C, Gebhardt K, Gruenberger B, Steinrueck V, Zommer H, Freudenthaler H, Roka S, Gruenberger T
Number of Citations: 1
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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