Part of Thermo Fisher Scientific

Thrombospondin I Monoclonal Antibody (A65M)

Thrombospondin I Antibody for ELISA, IHC, IP

Synonyms:
THBS1, TSP, TSP1
Entrez Gene ID:
(Human) 7057
UniProt ID:
P07996
Details
Host / Isotype: Mouse / IgG1, kappa
Class: Monoclonal
Type: Antibody
Clone: A65M
Species Reactivity: Human (Hu) Bovine (Bv)
Immunogen: Extracellular matrix materila fom cultured bovine corneal endothelial cells
Ordering Information
Pierce Thrombospondin I Monoclonal Antibody (A65M)
Product Number Pkg. Size Price Purchase
CSI 002-65-02 200 µg $545.00
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Storage: 4º C, store in dark
Form: 200 µg of Protein A purified antibody (1 mg/ml) in 0.01 M phosphate buffer, with 0.5 M NaCl and 15 mM sodium azide; pH 7.4.


Applications Dilution *
ELISA (ELISA) 1:200
Immunohistochemistry (IHC) 1:200
Immunoprecipitation (IP) Assay dependent
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
CSI 002-65-02 is highly specific for the low-calcium conformation of thrombospondin. There is no evidence for
cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin). CSI 002-65-02 cross-reacts with human thrombospondin. Other species have not been tested.
 
CSI 002-65-02 has been successfully used in ELISA, Immunohistochemistry and Immunoprecipitation procedures.
CSI 002-65 can also be used to probe for the low-calcium conformation of thrombospondin.

The CSI 002-65-02 immunogen is bovine corneal endothelial cells on beads.

The CSI 002-65-02 epitope is only present in thrombospondin prepared with low levels of calcium.
General Information
Thrombospondin is a regulator of many biological processes including cell growth, adhesion, migration, platelet aggregation, and fibrin deposition and lysis. It interacts with a number of plasma proteins such as fibrinogen and plasminogen and co-polymerizes with fibrin in clot formation. Thrombospondin also has multiple binding sites that interact with molecules such as fibronectin, collagens, laminin and heparan sulphate proteoglycans as well as binding growth factors such as TGF-beta1. Thrombospondin exerts an anti-adhesive effect which leads to cell rounding and detachment.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)