* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Antigen affinity chromatography
PBS, pH 7.6,
<0.1% sodium azide
4° C, do not freeze
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining, using an EDTA buffer for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is thymus tissue.
Terminal deoxynucleotidyl transferase (TdT) is a nuclear DNA polymerase responsible for the template-independent addition of N-nucleotides. It is expressed in normal hematopoietic cells, normal cortical thymocytes (immature, pre-B, pre-T lymphoid cells), and megakaryocytes of the bone marrow as well as acute lymphoblastic leukemia/lymphoma cells.
TdT Antibody (PA5-32610) in IHC TdT_PA5-32610_Antibody_Immunohistochemistry.jpg
Immunohistochemistry with anti-TdT Polyclonal Antibody (PA5-32610)