Tau Antibody (HT7)

Tau Monoclonal Antibody for Western blot, IF, ICC, IHC (P), ELISA

>> See 39 other antibodies for MAPT (G protein beta1/gamma2 subunit-interacting factor 1; PHF-tau; neurofibrillary tangle protein; paired helical filament-tau)
Synonyms:
G protein beta1/gamma2 subunit-interacting factor 1; PHF-tau; neurofibrillary tangle protein; paired helical filament-tau
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Mouse / IgG1, kappa
Class: Monoclonal
Type: Antibody
Clone: HT7
Tested Species Reactivity: Human (Hu), Bovine (Bv)
Published Species Reactivity: Human (Hu), Mouse (Ms), Rat (Rt)
Immunogen: Purified human Tau, epitope human Tau between residue 159 and 163 (numbering according to human Tau40), corresponding to the amino acid sequence PPGQK.
Ordering Information
Pierce Tau Antibody (HT7)
Product #
MN1000
Size
100 ug
Price
$299.00
Purchase
Add Tau Antibody (HT7) to your cart.
Tested Applications Dilution *
Western Blot (WB) Assay Dependent
Immunofluorescence (IF) 1:500-1:2000
Immunocytochemistry (ICC) 1:500-1:2000
Immunohistochemistry (Paraffin) (IHC (P)) 1:500-1:2000
ELISA (ELISA) Assay Dependent
Published Applications Dilution
Western Blot (WB) See publications below
Immunocytochemistry (ICC) See publications below
Immunohistochemistry (IHC) See publications below
Flow Cytometry (FACS) See publications below
Immunoprecipitation (IP) See publications below
ELISA (ELISA) See publications below
Blocking Assay (BLOCK) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 0.2mg/ml
Purification: Protein A
Storage Buffer: PBS
Preservative: no preservative
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
MN1000 targets Tau in ELISA, IF, ICC, IHC (P), and WB applications and shows reactivity with Bovine, and Human samples.

The MN1000 immunogen is purified human Tau, epitope human Tau between residue 159 and 163 (numbering according to human Tau40), corresponding to the amino acid sequence PPGQK.

MN1000 detects Tau which has a predicted molecular weight of approximately 79 kDa.
General Information
Paired helical filament (PHF) is a major component of the neurofibrillary tangles involved in the pathology of Alzheimer’s disease. PHFs are composed of the microtubule-associated protein tau in a hyper-phosphorylated state (ref1). Tau protein is produced by a single gene expressed predominantly in neurons. The Tau gene undergoes complex alternative splicing, yielding six different isoforms of tau in the adult brain. Following translation, the tau protein can be further modified by phosphorylation at several different sites
Product Images
  • Tau Antibody (MN1000) in WB
    Tau_Antibody_MN1000_Western-blot_1_20131210084914.jpg

    Western Blot with anti-Tau Monoclonal Antibody [HT7] (MN1000) (Tau_Antibody_MN1000_Western-blot_1_20131210084914.jpg)

    Western Blot with anti-Tau Monoclonal Antibody [HT7] (MN1000)

    Western blot analysis of human Tau was performed by loading 15ug of sarkosyl-soluble cortex lysate from transgenic (3xTg-AD) mice that express human Tau per well onto an SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% milk + 0.5% BSA in PBST buffer for 1 hour at room temperature. The membrane was probed with a Tau monoclonal antibody (Product # MN1000) at a dilution of 1:1000 at 4ºC overnight, washed in PBST, and probed with an HRP-conjugated anti-mouse IgG secondary antibody at a dilution of 1:60,000. Detection was performed using a chemiluminescent substrate. Data courtesy of the Innovators Program.
  • Tau Antibody (MN1000) in IF
    Tau_Antibody_MN1000_Immunohistochemistry_1_20131210084853.jpg

    Immunofluorescence with anti-Tau Monoclonal Antibody [HT7] (MN1000) (Tau_Antibody_MN1000_Immunohistochemistry_1_20131210084853.jpg)

    Immunofluorescence with anti-Tau Monoclonal Antibody [HT7] (MN1000)

    Immunohistochemistry was performed on paraformaldehyde-fixed free-floating hippocampus tissue sections from transgenic (3xTg-AD) mice that express human Tau. Tissues were blocked in 5% normal horse serum and 0.4% Triton X-100 for 60 minutes at room temperature and probed with a Tau monoclonal antibody (Product # MN1000) at a dilution of 1:1000 at 4ºC overnight. Tissues were washed extensively with PBS. Detection was performed using a fluorophore-conjugated anti-mouse IgG secondary antibody at a dilution of 1:1000. Tissues were visualized by fluorescence microscopy. Data courtesy of the Innovators Program.
Publications:
Western Blot
  Species / Dilution Summary
  Hu / Not Cited
MN1000 was used in immunohistochemistry and western blot to investigate the association between Abeta and tau in transgenic mice

J Neurochem. 2007 Aug;102(4):1053-63.
"Genetically augmenting tau levels does not modulate the onset or progression of Abeta pathology in transgenic mice."
Author(s): Oddo S, Caccamo A, Cheng D, Jouleh B, Torp R, LaFerla FM
Number of Citations: 1
(See PubMed article )
  Hu / 0
MN1000 was used in western blot to quantify the abeta and tau pathologies in mild cognitive impairment and Alzheimer disease

J Alzheimers Dis. 2007 Dec;12(4):377-90.
"Biochemical characterization of Abeta and tau pathologies in mild cognitive impairment and Alzheimer's disease."
Author(s): Tremblay C, Pilote M, Phivilay A, Emond V, Bennett DA, Calon F
Number of Citations: 2
(See PubMed article )
  Hu / Not Cited
MN1000 was used in western blot to investigate familial Alzheimer disease with presenilin mutations

Mol Neurodegener. 2008 Nov 20;3():20.
"Histopathological and molecular heterogeneity among individuals with dementia associated with Presenilin mutations."
Author(s): Maarouf CL, Daugs ID, Spina S, Vidal R, Kokjohn TA, Patton RL, Kalback WM, Luehrs DC, Walker DG, Castaño EM, Beach TG, Ghetti B, Roher AE
Number of Citations: 1
(See PubMed article )
  Hu / 1:1000
MN1000 was used in blocking or activating experiment and western blot to study the transmission and spreading of tauopathy in transgenic mouse brain

Nat Cell Biol. 2009 Jul;11(7):909-13.
"Transmission and spreading of tauopathy in transgenic mouse brain."
Author(s): Clavaguera F, Bolmont T, Crowther RA, Abramowski D, Frank S, Probst A, Fraser G, Stalder AK, Beibel M, Staufenbiel M, Jucker M, Goedert M, Tolnay M
Number of Citations: 91
(See PubMed article )
  Hu / 0
MN1000 was used in western blot to study the effect of alpha-synuclein on proteasome function

Biochemistry. 2009 Aug 25;48(33):8014-22.
"A cellular model to monitor proteasome dysfunction by alpha-synuclein."
Author(s): Nonaka T, Hasegawa M
Number of Citations: 1
(See PubMed article )
  Hu / 0
MN1000 was used in immunocytochemistry and western blot to study the generation of pathological-like tau assemblies at the plasma membrane

J Alzheimers Dis. 2009;18(4):919-33.
"Pathological-like assembly of tau induced by a paired helical filament core expressed at the plasma membrane."
Author(s): Campos-Peña V, Tapia-Ramírez J, Sánchez-Torres C, Meraz-Rios MA
Number of Citations: 0
(See PubMed article )
  Hu / Not Cited
MN1000 was used in immunocytochemistry, immunocytochemistry, western blot, and western blot to investigate the temporal expression profiles of amyloid-beta, APP and tau in primary cortical neurons derived from the 3xTg-AD mouse model

Cell Mol Neurobiol. 2010 May;30(4):577-90.
"Profile for amyloid-beta and tau expression in primary cortical cultures from 3xTg-AD mice."
Author(s): Vale C, Alonso E, Rubiolo JA, Vieytes MR, LaFerla FM, Giménez-Llort L, Botana LM
Number of Citations: 1
(See PubMed article )
  Hu / 1:5000
MN1000 was used in western blot to investigate the effect of memantine on Alzheimer disease treatment in mice

Am J Pathol. 2010 Feb;176(2):870-80.
"Memantine improves cognition and reduces Alzheimer's-like neuropathology in transgenic mice."
Author(s): Martinez-Coria H, Green KN, Billings LM, Kitazawa M, Albrecht M, Rammes G, Parsons CG, Gupta S, Banerjee P, LaFerla FM
Number of Citations: 1
(See PubMed article )
  Hu / 0
MN1000 was used in western blot to evaluate the transfection vector for fluorescent fusion protei expression in 293T and SH-SY5Y cells

Plasmid. 2010 May;63(3):155-60.
"Gateway-compatible lentiviral transfer vectors for ubiquitin promoter driven expression of fluorescent fusion proteins."
Author(s): Krupka N, Strappe P, Götz J, Ittner LM
Number of Citations: 1
(See PubMed article )
  Hu / 1:1000
MN1000 was used in western blot to investigate the effect of GLP-1 receptor stimulation on amyloid-beta peptide accumulation and cytotoxicity in Alzheimer disease model

J Alzheimers Dis. 2010;19(4):1205-19.
"GLP-1 receptor stimulation reduces amyloid-beta peptide accumulation and cytotoxicity in cellular and animal models of Alzheimer's disease."
Author(s): Li Y, Duffy KB, Ottinger MA, Ray B, Bailey JA, Holloway HW, Tweedie D, Perry T, Mattson MP, Kapogiannis D, Sambamurti K, Lahiri DK, Greig NH
Number of Citations: 1
(See PubMed article )
  Hu / 0
MN1000 was used in flow cytometry, immunocytochemistry, and western blot to investigate the effect of sodium selenate on tau pathology

Proc Natl Acad Sci U S A. 2010 Aug 3;107(31):13888-93.
"Sodium selenate mitigates tau pathology, neurodegeneration, and functional deficits in Alzheimer's disease models."
Author(s): van Eersel J, Ke YD, Liu X, Delerue F, Kril JJ, Götz J, Ittner LM
Number of Citations: 1
(See PubMed article )
  Hu / 0
MN1000 was used in immunohistochemistry and western blot to investigate the function of tau in neuronal dendrites for A-beta toxicity

Cell. 2010 Aug 6;142(3):387-97.
"Dendritic function of tau mediates amyloid-beta toxicity in Alzheimer's disease mouse models."
Author(s): Ittner LM, Ke YD, Delerue F, Bi M, Gladbach A, van Eersel J, Wölfing H, Chieng BC, Christie MJ, Napier IA, Eckert A, Staufenbiel M, Hardeman E, Götz J
Number of Citations: 1
(See PubMed article )
  Hu / 1:1000
MN1000 was used in immunohistochemistry and western blot to investigate the effect of deficits in adult neurogenesis on premature cognitive decline in Alzheimer disease

Eur J Neurosci. 2010 Sep;32(6):905-20.
"Widespread deficits in adult neurogenesis precede plaque and tangle formation in the 3xTg mouse model of Alzheimer's disease."
Author(s): Hamilton LK, Aumont A, Julien C, Vadnais A, Calon F, Fernandes KJ
Number of Citations: 1
(See PubMed article )
  Hu / 1:3000
MN1000 was used in immunohistochemistry and western blot to investigate the effect of methylene blue on Alzheimer disease treatment in a mouse model

Brain Pathol. 2011 Mar;21(2):140-9.
"Methylene blue reduces a? levels and rescues early cognitive deficit by increasing proteasome activity."
Author(s): Medina DX, Caccamo A, Oddo S
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
MN1000 was used in immunocytochemistry and western blot to investigate the role of nuclear Tau on early stress response and neuronal DNA protection

J Biol Chem. 2011 Feb 11;286(6):4566-75.
"Nuclear tau, a key player in neuronal DNA protection."
Author(s): Sultan A, Nesslany F, Violet M, Bégard S, Loyens A, Talahari S, Mansuroglu Z, Marzin D, Sergeant N, Humez S, Colin M, Bonnefoy E, Buée L, Galas MC
Number of Citations: 10
(See PubMed article )
  Hu / 0
MN1000 was used in western blot to investigate the influence of hippocampal tauopathy in learning, memory and neural functions in transgenic mice

Neurobiol Learn Mem. 2011 Mar;95(3):296-304.
"Hippocampal tauopathy in tau transgenic mice coincides with impaired hippocampus-dependent learning and memory, and attenuated late-phase long-term depression of synaptic transmission."
Author(s): Van der Jeugd A, Ahmed T, Burnouf S, Belarbi K, Hamdame M, Grosjean ME, Humez S, Balschun D, Blum D, Buée L, D'Hooge R
Number of Citations: 1
(See PubMed article )
  Hu / 0
MN1000 was used in immunohistochemistry and western blot to study the effect of grape seed polyphenols on the ultrastructure of paired helical filaments derived from Alzheimer's disease brains

Neurobiol Aging. 2012 Jul;33(7):1427-39.
"Ultrastructural alterations of Alzheimer's disease paired helical filaments by grape seed-derived polyphenols."
Author(s): Ksiezak-Reding H, Ho L, Santa-Maria I, Diaz-Ruiz C, Wang J, Pasinetti GM
Number of Citations: 0
(See PubMed article )
  Hu / Not Cited
MN1000 was used in western blot to identify the relationship between acetylcholinesterase and P-tau expression in neurodegeneration

Neurobiol Aging. 2012 Mar;33(3):624.e23-34.
"Altered expression of brain acetylcholinesterase in FTDP-17 human tau transgenic mice."
Author(s): Silveyra MX, García-Ayllón MS, de Barreda EG, Small DH, Martínez S, Avila J, Sáez-Valero J
Number of Citations: 1
(See PubMed article )
  Hu / 1:1000
MN1000 was used in western blot to investigate the effects of wild type and P301L mutant tau on neuro-inflammation and alpha-synuclein accumulation in a rat model

Mol Cell Neurosci. 2012 Jan;49(1):44-53.
"Wild type and P301L mutant Tau promote neuro-inflammation and ?-Synuclein accumulation in lentiviral gene delivery models."
Author(s): Khandelwal PJ, Dumanis SB, Herman AM, Rebeck GW, Moussa CE
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
Hu / 0
MN1000 was used in immunohistochemistry and western blot to investigate the effect of blocking IL-1 signaling in an Alzheimer disease mouse model

J Immunol. 2011 Dec 15;187(12):6539-49.
"Blocking IL-1 signaling rescues cognition, attenuates tau pathology, and restores neuronal ?-catenin pathway function in an Alzheimer's disease model."
Author(s): Kitazawa M, Cheng D, Tsukamoto MR, Koike MA, Wes PD, Vasilevko V, Cribbs DH, LaFerla FM
Number of Citations: 2
(See PubMed article )
  Hu / 0
MN1000 was used in western blot to investigate the effect of prolonged NO treatment on the aggregation of tau in SH-SY5Y cells

Neurosci Lett. 2012 Feb 21;510(1):48-52.
"Prolonged nitric oxide treatment induces tau aggregation in SH-SY5Y cells."
Author(s): Takahashi M, Chin Y, Nonaka T, Hasegawa M, Watanabe N, Arai T
Number of Citations: 1
(See PubMed article )
  Hu / 0
MN1000 was used in western blot to investigate the important roles of LRRK2 in phosphorylation-mediated dissociation of tau from microtubules

PLoS One. 2012;7(1):e30834.
"LRRK2 phosphorylates tubulin-associated tau but not the free molecule: LRRK2-mediated regulation of the tau-tubulin association and neurite outgrowth."
Author(s): Kawakami F, Yabata T, Ohta E, Maekawa T, Shimada N, Suzuki M, Maruyama H, Ichikawa T, Obata F
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
MN1000 was used in western blot to study the beneficial effects of a novel calpain inhibitor on Alzheimers's disease pathology and cognitive decline in transgenic mouse model

Am J Pathol. 2012 Aug;181(2):616-25.
"Calpain inhibitor A-705253 mitigates Alzheimer's disease-like pathology and cognitive decline in aged 3xTgAD mice."
Author(s): Medeiros R, Kitazawa M, Chabrier MA, Cheng D, Baglietto-Vargas D, Kling A, Moeller A, Green KN, LaFerla FM
Number of Citations: 1
(See PubMed article )
  Hu / 1:2000
MN1000 was used in western blot to study the clinical and molecular characteristics of a novel P332S tau mutation

J Alzheimers Dis. 2012;31(4):741-9.
"Clinical, neuropathological, and biochemical characterization of the novel tau mutation P332S."
Author(s): Deramecourt V, Lebert F, Maurage CA, Fernandez-Gomez FJ, Dujardin S, Colin M, Sergeant N, Buée-Scherrer V, Clot F, Ber IL, Brice A, Pasquier F, Buée L
Number of Citations: 0
(See PubMed article )
  Hu / 0
MN1000 was used in immunohistochemistry and western blot to study the role of soluble total and hyperphosphorylated tau in brain pathology and behaviour in a transgenic mouse model

Neurodegener Dis. 2012 Jul 10;():.
"Elevated Levels of Soluble Total and Hyperphosphorylated Tau Result in Early Behavioral Deficits and Distinct Changes in Brain Pathology in a New Tau Transgenic Mouse Model."
Author(s): Flunkert S, Hierzer M, Löffler T, Rabl R, Neddens J, Duller S, Schofield EL, Ward MA, Posch M, Jungwirth H, Windisch M, Hutter-Paier B
Number of Citations: 0
(See PubMed article )
  Hu / Not Cited
MN1000 was used in western blot to study the biochemical/molecular changes associated with dementia pugilistica

J Neurotrauma. 2013 Jun 1;30(11):981-97.
"Neurochemical profile of dementia pugilistica."
Author(s): Kokjohn TA, Maarouf CL, Daugs ID, Hunter JM, Whiteside CM, Malek-Ahmadi M, Rodriguez E, Kalback W, Jacobson SA, Sabbagh MN, Beach TG, Roher AE
Number of Citations: 1
(See PubMed article )
  Hu / 1:40000
MN1000 was used in western blot to study the association of GSK3 activity with tau phosphorylation and neuronal tyrosine phosphorylation in a murine model expressing human P301L tau

Neurobiol Aging. 2013 May;34(5):1369-79.
"Active glycogen synthase kinase-3 and tau pathology-related tyrosine phosphorylation in pR5 human tau transgenic mice."
Author(s): Köhler C, Dinekov M, Götz J
Number of Citations: 3
(See PubMed article )
  Hu / Not Cited
MN1000 was used in western blot to study the ability of PINCH in bind and stabilize hyperphoshorylated tau

PLoS One. 2013;8(3):e58232.
"PINCH in the cellular stress response to tau-hyperphosphorylation."
Author(s): Ozdemir AY, Rom I, Kovalevich J, Yen W, Adiga R, Dave RS, Langford D
Number of Citations: 0
(See PubMed article )
  Ms / Not Cited
MN1000 was used in immunocytochemistry, immunocytochemistry, western blot, and western blot to investigate the temporal expression profiles of amyloid-beta, APP and tau in primary cortical neurons derived from the 3xTg-AD mouse model

Cell Mol Neurobiol. 2010 May;30(4):577-90.
"Profile for amyloid-beta and tau expression in primary cortical cultures from 3xTg-AD mice."
Author(s): Vale C, Alonso E, Rubiolo JA, Vieytes MR, LaFerla FM, Giménez-Llort L, Botana LM
Number of Citations: 1
(See PubMed article )
  Ms / 1:5000
MN1000 was used in western blot to study the deleterious effects of muscarinic M1 receptor knockout on Alzheimer's disease pathology and cognitive decline in a transgenic mouse model

Am J Pathol. 2011 Aug;179(2):980-91.
"Loss of muscarinic M1 receptor exacerbates Alzheimer's disease-like pathology and cognitive decline."
Author(s): Medeiros R, Kitazawa M, Caccamo A, Baglietto-Vargas D, Estrada-Hernandez T, Cribbs DH, Fisher A, LaFerla FM
Number of Citations: 5
(See PubMed article )
  Ms / 1:3000
MN1000 was used in immunohistochemistry and western blot to study whether anesthesia accelerates disease pathology in presymptomatic Alzheimer's disease in a transgenic mouse model

Alzheimers Dement. 2011 Sep;7(5):521-531.e1.
"Anesthesia in presymptomatic Alzheimer's disease: a study using the triple-transgenic mouse model."
Author(s): Tang JX, Mardini F, Caltagarone BM, Garrity ST, Li RQ, Bianchi SL, Gomes O, Laferla FM, Eckenhoff RG, Eckenhoff MF
Number of Citations: 4
(See PubMed article )
  Ms / 1:2000
MN1000 was used in western blot to study whether tau pathology is associated with BDNF downregulation in a transgenic mouse model

Curr Alzheimer Res. 2012 May;9(4):406-10.
"Hippocampal BDNF expression in a tau transgenic mouse model."
Author(s): Burnouf S, Belarbi K, Troquier L, Derisbourg M, Demeyer D, Leboucher A, Laurent C, Hamdane M, Buee L, Blum D
Number of Citations: 0
(See PubMed article )
  Ms / Not Cited
MN1000 was used in western blot to study the tau-induced loss of synapses in a murine model overexpressing the P301L mutant tau

J Comp Neurol. 2013 Apr 15;521(6):1334-53.
"Synaptic alterations in the rTg4510 mouse model of tauopathy."
Author(s): Kopeikina KJ, Polydoro M, Tai HC, Yaeger E, Carlson GA, Pitstick R, Hyman BT, Spires-Jones TL
Number of Citations: 6
(See PubMed article )
  Rt / Not Cited
MN1000 was used in western blot to study the contribution of heat-shock protein 90 towards tauopathies

Proc Natl Acad Sci U S A. 2007 May 29;104(22):9511-6.
"Roles of heat-shock protein 90 in maintaining and facilitating the neurodegenerative phenotype in tauopathies."
Author(s): Luo W, Dou F, Rodina A, Chip S, Kim J, Zhao Q, Moulick K, Aguirre J, Wu N, Greengard P, Chiosis G
Number of Citations: 1
(See PubMed article )
  Rt / 1:400
MN1000 was used in western blot to study the mechanism for the induction of tau phosphorylation by amyloid beta peptide

J Neurosci Res. 2008 Jul;86(9):2091-9.
"ER stress is involved in Abeta-induced GSK-3beta activation and tau phosphorylation."
Author(s): Resende R, Ferreiro E, Pereira C, Oliveira CR
Number of Citations: 1
(See PubMed article )
Immunocytochemistry
  Species / Dilution Summary
  Hu / 0
MN1000 was used in immunocytochemistry and western blot to study the generation of pathological-like tau assemblies at the plasma membrane

J Alzheimers Dis. 2009;18(4):919-33.
"Pathological-like assembly of tau induced by a paired helical filament core expressed at the plasma membrane."
Author(s): Campos-Peña V, Tapia-Ramírez J, Sánchez-Torres C, Meraz-Rios MA
Number of Citations: 0
(See PubMed article )
  Hu / 1:200
MN1000 was used in immunocytochemistry, immunocytochemistry, western blot, and western blot to investigate the temporal expression profiles of amyloid-beta, APP and tau in primary cortical neurons derived from the 3xTg-AD mouse model

Cell Mol Neurobiol. 2010 May;30(4):577-90.
"Profile for amyloid-beta and tau expression in primary cortical cultures from 3xTg-AD mice."
Author(s): Vale C, Alonso E, Rubiolo JA, Vieytes MR, LaFerla FM, Giménez-Llort L, Botana LM
Number of Citations: 1
(See PubMed article )
  Hu / 0
MN1000 was used in flow cytometry, immunocytochemistry, and western blot to investigate the effect of sodium selenate on tau pathology

Proc Natl Acad Sci U S A. 2010 Aug 3;107(31):13888-93.
"Sodium selenate mitigates tau pathology, neurodegeneration, and functional deficits in Alzheimer's disease models."
Author(s): van Eersel J, Ke YD, Liu X, Delerue F, Kril JJ, Götz J, Ittner LM
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
MN1000 was used in immunocytochemistry and western blot to investigate the role of nuclear Tau on early stress response and neuronal DNA protection

J Biol Chem. 2011 Feb 11;286(6):4566-75.
"Nuclear tau, a key player in neuronal DNA protection."
Author(s): Sultan A, Nesslany F, Violet M, Bégard S, Loyens A, Talahari S, Mansuroglu Z, Marzin D, Sergeant N, Humez S, Colin M, Bonnefoy E, Buée L, Galas MC
Number of Citations: 10
(See PubMed article )
  Ms / 1:200
MN1000 was used in immunocytochemistry, immunocytochemistry, western blot, and western blot to investigate the temporal expression profiles of amyloid-beta, APP and tau in primary cortical neurons derived from the 3xTg-AD mouse model

Cell Mol Neurobiol. 2010 May;30(4):577-90.
"Profile for amyloid-beta and tau expression in primary cortical cultures from 3xTg-AD mice."
Author(s): Vale C, Alonso E, Rubiolo JA, Vieytes MR, LaFerla FM, Giménez-Llort L, Botana LM
Number of Citations: 1
(See PubMed article )
Immunohistochemistry
  Species / Dilution Summary
  Hu / Not Cited
MN1000 was used in immunohistochemistry and western blot to investigate the association between Abeta and tau in transgenic mice

J Neurochem. 2007 Aug;102(4):1053-63.
"Genetically augmenting tau levels does not modulate the onset or progression of Abeta pathology in transgenic mice."
Author(s): Oddo S, Caccamo A, Cheng D, Jouleh B, Torp R, LaFerla FM
Number of Citations: 1
(See PubMed article )
  Hu / 1:400
MN1000 was used in immunohistochemistry to analyze the cholinergic pathology in a tauopathy model

Brain Res. 2010 Aug 6;1347():111-24.
"Analysis of the cholinergic pathology in the P301L tau transgenic pR5 model of tauopathy."
Author(s): Köhler C, Bista P, Götz J, Schröder H
Number of Citations: 1
(See PubMed article )
  Hu / 0
MN1000 was used in immunohistochemistry and western blot to investigate the function of tau in neuronal dendrites for A-beta toxicity

Cell. 2010 Aug 6;142(3):387-97.
"Dendritic function of tau mediates amyloid-beta toxicity in Alzheimer's disease mouse models."
Author(s): Ittner LM, Ke YD, Delerue F, Bi M, Gladbach A, van Eersel J, Wölfing H, Chieng BC, Christie MJ, Napier IA, Eckert A, Staufenbiel M, Hardeman E, Götz J
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
MN1000 was used in immunohistochemistry to develop a FRET-based method to detect pathogenic forms of tau.

J Neurosci Methods. 2010 Sep 30;192(1):127-37.
"Spatially pathogenic forms of tau detected in Alzheimer's disease brain tissue by fluorescence lifetime-based Förster resonance energy transfer."
Author(s): Larionov S, Wielgat P, Wang Y, Thal DR, Neumann H
Number of Citations: 0
(See PubMed article )
  Hu / 1:200
MN1000 was used in immunohistochemistry to investigate the effect of Abeta(1-42) on the survival of mOP cells in Alzheimer disease mouse model

Am J Pathol. 2010 Sep;177(3):1422-35.
"Early oligodendrocyte/myelin pathology in Alzheimer's disease mice constitutes a novel therapeutic target."
Author(s): Desai MK, Mastrangelo MA, Ryan DA, Sudol KL, Narrow WC, Bowers WJ
Number of Citations: 1
(See PubMed article )
  Hu / 1:200
MN1000 was used in immunohistochemistry and western blot to investigate the effect of deficits in adult neurogenesis on premature cognitive decline in Alzheimer disease

Eur J Neurosci. 2010 Sep;32(6):905-20.
"Widespread deficits in adult neurogenesis precede plaque and tangle formation in the 3xTg mouse model of Alzheimer's disease."
Author(s): Hamilton LK, Aumont A, Julien C, Vadnais A, Calon F, Fernandes KJ
Number of Citations: 1
(See PubMed article )
  Hu / 1:3000
MN1000 was used in immunohistochemistry and western blot to investigate the effect of methylene blue on Alzheimer disease treatment in a mouse model

Brain Pathol. 2011 Mar;21(2):140-9.
"Methylene blue reduces a? levels and rescues early cognitive deficit by increasing proteasome activity."
Author(s): Medina DX, Caccamo A, Oddo S
Number of Citations: 1
(See PubMed article )
  Hu / 1:100
MN1000 was used in immunohistochemistry to investigate the dendritic spine loss during the progression of Alzheimer disease in a mouse model

PLoS One. 2010 Nov 16;5(11):e15477.
"Multiple events lead to dendritic spine loss in triple transgenic Alzheimer's disease mice."
Author(s): Bittner T, Fuhrmann M, Burgold S, Ochs SM, Hoffmann N, Mitteregger G, Kretzschmar H, LaFerla FM, Herms J
Number of Citations: 8
(See PubMed article )
  Hu / 0
MN1000 was used in immunohistochemistry and western blot to study the effect of grape seed polyphenols on the ultrastructure of paired helical filaments derived from Alzheimer's disease brains

Neurobiol Aging. 2012 Jul;33(7):1427-39.
"Ultrastructural alterations of Alzheimer's disease paired helical filaments by grape seed-derived polyphenols."
Author(s): Ksiezak-Reding H, Ho L, Santa-Maria I, Diaz-Ruiz C, Wang J, Pasinetti GM
Number of Citations: 0
(See PubMed article )
  Hu / Not Cited
MN1000 was used in immunohistochemistry to detect Tau protein in cerebrospinal fluid

J Alzheimers Dis. 2011;24 Suppl 2():127-41.
"Tau transgenic mice as models for cerebrospinal fluid tau biomarkers."
Author(s): Barten DM, Cadelina GW, Hoque N, DeCarr LB, Guss VL, Yang L, Sankaranarayanan S, Wes PD, Flynn ME, Meredith JE, Ahlijanian MK, Albright CF
Number of Citations: 6
(See PubMed article )
  Hu / 1:100
MN1000 was used in immunohistochemistry to investigate the changes of tau pattern in some older patients with dementia

Acta Neuropathol. 2011 Aug;122(2):205-22.
"A peculiar constellation of tau pathology defines a subset of dementia in the elderly."
Author(s): Kovacs GG, Molnár K, László L, Ströbel T, Botond G, Hönigschnabl S, Reiner-Concin A, Palkovits M, Fischer P, Budka H
Number of Citations: 1
(See PubMed article )
  Hu / 1:1000
MN1000 was used in immunohistochemistry to investigate the influence of tau oligomer on neuronal morphology and function in mice

Mol Neurodegener. 2011 Jun 6;6():39.
"Tau oligomers impair memory and induce synaptic and mitochondrial dysfunction in wild-type mice."
Author(s): Lasagna-Reeves CA, Castillo-Carranza DL, Sengupta U, Clos AL, Jackson GR, Kayed R
Number of Citations: 1
(See PubMed article )
  Hu / 0.2 ug/mL
Hu / 1 ug/ml
MN1000 was used in immunohistochemistry to investigate the effect of traumatic brain injury on the development of Alzheimer disease pathology

J Neurosci. 2011 Jun 29;31(26):9513-25.
"Controlled cortical impact traumatic brain injury in 3xTg-AD mice causes acute intra-axonal amyloid-? accumulation and independently accelerates the development of tau abnormalities."
Author(s): Tran HT, LaFerla FM, Holtzman DM, Brody DL
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
MN1000 was used in immunohistochemistry to investigate the neuropathological changes in dementia pugilistica patients

J Neurotrauma. 2012 Apr 10;29(6):1054-70.
"Frontal cortex neuropathology in dementia pugilistica."
Author(s): Saing T, Dick M, Nelson PT, Kim RC, Cribbs DH, Head E
Number of Citations: 1
(See PubMed article )
  Hu / 1:2000
MN1000 was used in immunohistochemistry and western blot to investigate the effect of blocking IL-1 signaling in an Alzheimer disease mouse model

J Immunol. 2011 Dec 15;187(12):6539-49.
"Blocking IL-1 signaling rescues cognition, attenuates tau pathology, and restores neuronal ?-catenin pathway function in an Alzheimer's disease model."
Author(s): Kitazawa M, Cheng D, Tsukamoto MR, Koike MA, Wes PD, Vasilevko V, Cribbs DH, LaFerla FM
Number of Citations: 2
(See PubMed article )
  Hu / 1:4000
MN1000 was used in immunohistochemistry to study the role of oxidative stress in Alzheimer's disease pathogenesis and progression

J Alzheimers Dis. 2012;30(1):183-208.
"Effects of the superoxide dismutase/catalase mimetic EUK-207 in a mouse model of Alzheimer's disease: protection against and interruption of progression of amyloid and tau pathology and cognitive decline."
Author(s): Clausen A, Xu X, Bi X, Baudry M
Number of Citations: 0
(See PubMed article )
  Hu / 1:200
Hu / 1:200
MN1000 was used in immunohistochemistry and western blot to study the role of soluble total and hyperphosphorylated tau in brain pathology and behaviour in a transgenic mouse model

Neurodegener Dis. 2012 Jul 10;():.
"Elevated Levels of Soluble Total and Hyperphosphorylated Tau Result in Early Behavioral Deficits and Distinct Changes in Brain Pathology in a New Tau Transgenic Mouse Model."
Author(s): Flunkert S, Hierzer M, Löffler T, Rabl R, Neddens J, Duller S, Schofield EL, Ward MA, Posch M, Jungwirth H, Windisch M, Hutter-Paier B
Number of Citations: 0
(See PubMed article )
  Hu / 1:1000
MN1000 was used in immunohistochemistry to study whether tau pathology caused by overexpression of mutant P301L tau in the murine entorhinal-hippocampal network leads to cognitive defects

PLoS One. 2012;7(9):e45881.
"Human P301L-mutant tau expression in mouse entorhinal-hippocampal network causes tau aggregation and presynaptic pathology but no cognitive deficits."
Author(s): Harris JA, Koyama A, Maeda S, Ho K, Devidze N, Dubal DB, Yu GQ, Masliah E, Mucke L
Number of Citations: 4
(See PubMed article )
  Ms / 1:3000
MN1000 was used in immunohistochemistry and western blot to study whether anesthesia accelerates disease pathology in presymptomatic Alzheimer's disease in a transgenic mouse model

Alzheimers Dement. 2011 Sep;7(5):521-531.e1.
"Anesthesia in presymptomatic Alzheimer's disease: a study using the triple-transgenic mouse model."
Author(s): Tang JX, Mardini F, Caltagarone BM, Garrity ST, Li RQ, Bianchi SL, Gomes O, Laferla FM, Eckenhoff RG, Eckenhoff MF
Number of Citations: 4
(See PubMed article )
  Ms / 1:500
MN1000 was used in immunohistochemistry to study the ability of the GSKbeta inhibitor morin to reduce tau hyperphosphorylation

Neurobiol Dis. 2011 Nov;44(2):223-30.
"Morin attenuates tau hyperphosphorylation by inhibiting GSK3?."
Author(s): Gong EJ, Park HR, Kim ME, Piao S, Lee E, Jo DG, Chung HY, Ha NC, Mattson MP, Lee J
Number of Citations: 3
(See PubMed article )
  Ms / 1:1000
MN1000 was used in immunohistochemistry to study the ability of lysine-specific molecular tweezers to protect against Alzheimer's disease pathology

Brain. 2012 Dec;135(Pt 12):3735-48.
"Protection of primary neurons and mouse brain from Alzheimer's pathology by molecular tweezers."
Author(s): Attar A, Ripoli C, Riccardi E, Maiti P, Li Puma DD, Liu T, Hayes J, Jones MR, Lichti-Kaiser K, Yang F, Gale GD, Tseng CH, Tan M, Xie CW, Straudinger JL, Klärner FG, Schrader T, Frautschy SA, Grassi C, Bitan G
Number of Citations: 3
(See PubMed article )
  Ms / 1:400
MN1000 was used in immunohistochemistry to study whether the expression of mutant human tau in tau filament-forming mice recapitulates the pathological chages observed in Alzheimer's disease brainstem

Brain Res. 2013 Feb 25;1497():73-84.
"Pattern of tau hyperphosphorylation and neurotransmitter markers in the brainstem of senescent tau filament forming transgenic mice."
Author(s): Morcinek K, Köhler C, Götz J, Schröder H
Number of Citations: 0
(See PubMed article )
Flow Cytometry
  Species / Dilution Summary
  Hu / 0
MN1000 was used in flow cytometry, immunocytochemistry, and western blot to investigate the effect of sodium selenate on tau pathology

Proc Natl Acad Sci U S A. 2010 Aug 3;107(31):13888-93.
"Sodium selenate mitigates tau pathology, neurodegeneration, and functional deficits in Alzheimer's disease models."
Author(s): van Eersel J, Ke YD, Liu X, Delerue F, Kril JJ, Götz J, Ittner LM
Number of Citations: 1
(See PubMed article )
Immunoprecipitation
  Species / Dilution Summary
  Hu / 0
MN1000 was used in immunoprecipitation to identify and characterize a novel class of GSK3 beta inhibitors

Bioorg Med Chem Lett. 2011 Mar 1;21(5):1429-33.
"6-amino-4-(pyrimidin-4-yl)pyridones: novel glycogen synthase kinase-3? inhibitors."
Author(s): Coffman K, Brodney M, Cook J, Lanyon L, Pandit J, Sakya S, Schachter J, Tseng-Lovering E, Wessel M
Number of Citations: 1
(See PubMed article )
ELISA
  Species / Dilution Summary
  Hu / 2 ug/ml
MN1000 was used in ELISA to develop a novel ELISA assay for quantification of tau in cerebrospinal fluid

Neurosci Lett. 2007 May 17;418(2):186-9.
"Tau in cerebrospinal fluid: a sensitive sandwich enzyme-linked immunosorbent assay using tyramide signal amplification."
Author(s): Yamamori H, Khatoon S, Grundke-Iqbal I, Blennow K, Ewers M, Hampel H, Iqbal K
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
MN1000 was used in ELISA to study the sAPPb decrease in Alzheimer disease cortex

J Neurosci Res. 2011 Jun;89(6):822-32.
"Decrease in brain soluble amyloid precursor protein ? (sAPP?) in Alzheimer's disease cortex."
Author(s): Wu G, Sankaranarayanan S, Hsieh SH, Simon AJ, Savage MJ
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
MN1000 was used in ELISA to investigate the characteristics of TTBK1 in the brain of AD patients

Brain Pathol. 2013 Jul;23(4):378-89.
"Tau-tubulin kinase 1 expression, phosphorylation and co-localization with phospho-Ser422 tau in the Alzheimer's disease brain."
Author(s): Lund H, Cowburn RF, Gustafsson E, Strömberg K, Svensson A, Dahllund L, Malinowsky D, Sunnemark D
Number of Citations: 2
(See PubMed article )
Blocking Assay
  Species / Dilution Summary
  Hu / 0
MN1000 was used in blocking or activating experiment and western blot to study the transmission and spreading of tauopathy in transgenic mouse brain

Nat Cell Biol. 2009 Jul;11(7):909-13.
"Transmission and spreading of tauopathy in transgenic mouse brain."
Author(s): Clavaguera F, Bolmont T, Crowther RA, Abramowski D, Frank S, Probst A, Fraser G, Stalder AK, Beibel M, Staufenbiel M, Jucker M, Goedert M, Tolnay M
Number of Citations: 91
(See PubMed article )
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