TNF Alpha Antibody (E10011)
TNF Alpha Monoclonal Antibody for ELISA, Western blot
Synonyms:
TNF-a; TNF-alpha; cachectin; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor ligand superfamily member 2; tumor necrosis factor, alpha (TNF superfamily, member 2)
Entrez Gene ID:
UniProt ID:
Q06599
| Details | |
| Host / Isotype: | Rabbit / IgG1 |
| Class: | Monoclonal |
| Type: | Antibody |
| Clone: | E10011 |
| Species Reactivity: | Bovine (Bv) |
| Immunogen: | Recombinant bovine TNF? purified from Pichia pastoris |
| Ordering Information | ||||
| Pierce TNF Alpha Antibody (E10011) |
| Storage: | 4° C |
| Form: | 500 ug of purified antibody from mouse ascites fluid by Protein G affinity chromatography and Lyophilized in PBS with 5% trehalose without preservative or carrier protein |
| Applications | Dilution * |
| ELISA (ELISA) | Assay Dependent |
| Western Blot (WB) | Assay Dependent |
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* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
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| Product Specific Information |
| MBOTNFAI targets TNF Alpha in ELISA, and WB applications and shows reactivity with Bovine samples. The MBOTNFAI immunogen is recombinant bovine TNFa purified from Pichia pastoris. MBOTNFAI detects TNF Alpha which has a predicted molecular weight of approximately 26 kDa. The MBOTNFAI bovine TNFa monoclonal antibody (clone E10011) has successfully been paired as the coating antibody in a sandwich ELISA with biotinylated polyclonal detection antibody PBOTNFABI . Typical dilutions for sandwich ELISA include 1 µg/ml for coating and 0.125 - 0.25 µg/ml for detection. |
| General Information |
| This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. |
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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TNF Alpha Antibodies > TNF Alpha Monoclonal Antibody MBOTNFAI
