TNF Alpha Antibody (2TNF-H34A)

TNF Alpha Monoclonal Antibody for Western blot, ELISA

>> See 40 other antibodies for TNF (APC1 protein; TNF superfamily, member 2; TNF, macrophage-derived; TNF, monocyte-derived; TNF-a; cachectin; tumor necrosis factor alpha; tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha)
Synonyms:
APC1 protein; TNF superfamily, member 2; TNF, macrophage-derived; TNF, monocyte-derived; TNF-a; cachectin; tumor necrosis factor alpha; tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Mouse / IgG1, kappa
Class: Monoclonal
Type: Antibody
Clone: 2TNF-H34A
Tested Species Reactivity: Human (Hu)
Published Species Reactivity: Human (Hu)
Immunogen: Recombinant human TNF alpha
Ordering Information
Pierce TNF Alpha Antibody (2TNF-H34A)
Product #
M303
Size
500 µg
Price
$350.00
Purchase
Add TNF Alpha Antibody (2TNF-H34A) to your cart.
Tested Applications Dilution *
Western Blot (WB) Assay Dependent
ELISA (ELISA) Assay Dependent
Published Applications Dilution
ELISA (ELISA) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 1.01mg/ml
Purification: Protein G
Storage Buffer: PBS
Preservative: no preservative
Storage Conditions: -20°C
Product Specific Information
M303 targets TNF Alpha in ELISA, and WB applications and shows reactivity with Human samples. The M303 antibody (clone 2TNF-H34A) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody M302B (biotinylated conjugate of clone 2TNG-H33).

The M303 immunogen is recombinant human TNF alpha.

M303 detects TNF Alpha which has a predicted molecular weight of approximately 26 kDa.

The M303 TNFa antibody (clone 2TNF-H34A) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody M302B (biotinylated conjugate of clone 2TNF-H33) Typical dilutions for sandwich ELISA include 1 µg/ml for coating and 0.125 - 0.25 µg/ml for detection.

Antibody M303 (clone 2TNF-H34A) and biotinylated antibody M302B (clone 2TNF-H33) have successfully been used in combination with recombinant TNFa protein STNFA in ELISA applications.
General Information
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
Publications:
ELISA
  Species / Dilution Summary
  Hu / Not Cited
M303 was used in ELISA to assess the interferon-alpha production in peripheral blood mononuclear cell

J Exp Med. 1997 Jun 2;185(11):1987-95.
"Regulation of interleukin-12 by complement receptor 3 signaling."
Author(s): Marth T, Kelsall BL
Number of Citations: 42
(See PubMed article )
  Hu / 4 mg/ml
M303 was used in ELISA to study the role of inteferon gamma in EBV-transformed B cells

Viral Immunol. 2002;15(1):213-25.
"Interferon--gamma control of EBV-transformed B cells: a role for CD8+ T cells that poorly kill EBV-infected cells."
Author(s): Shi Y, Lutz CT
Number of Citations: 1
(See PubMed article )
  Hu / 2 ug/ml
M303 was used in ELISA to study the role of type 1 cytokine in chronic hepatosplenic schistosomiasis

Infect Immun. 2002 Nov;70(11):5903-12.
"Enhanced interleukin-12 and CD40 ligand activities but reduced Staphylococcus aureus Cowan 1-induced responses suggest a generalized and progressively impaired type 1 cytokine pattern for human schistosomiasis."
Author(s): Montenegro SM, Abath FG, Domingues AL, Melo WG, Morais CN, Coutinho EM, Mahanty S, Wynn TA
Number of Citations: 0
(See PubMed article )
  Hu / Not Cited
M303 was used in ELISA to investigate the role of plasmacytoid dendritic cells (pDCs) in adaptive immunity

Blood. 2003 May 1;101(9):3520-6.
"Activation of influenza virus-specific CD4+ and CD8+ T cells: a new role for plasmacytoid dendritic cells in adaptive immunity."
Author(s): Fonteneau JF, Gilliet M, Larsson M, Dasilva I, Münz C, Liu YJ, Bhardwaj N
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
M303 was used in ELISA to investigate the augmented production of Th1-type cytokines in HCV-infected cryoglobulinemic patients

J Hepatol. 2003 Feb;38(2):230-6.
"Enhanced monocyte Th1 cytokine production in HCV-infected cryoglobulinemic patients."
Author(s): Loffreda S, Muratori P, Muratori L, Mele L, Bianchi FB, Lenzi M
Number of Citations: 2
(See PubMed article )
  Hu / Not Cited
M303 was used in ELISA to investigate the generation of three-dimensional pannus-like tissues in vitro from single cell suspensions of synovial fluid cells from arthritis patients

Rheumatol Int. 2004 Mar;24(2):71-6.
"Generation of three-dimensional pannus-like tissues in vitro from single cell suspensions of synovial fluid cells from arthritis patients."
Author(s): Solomon S, Masilamani M, Mohanty S, Schwab JE, Boneberg EM, Illges H
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
M303 was used in ELISA to study the effect of etanercept treatment on proinflammatory cytokine-secreting peripheral blood mononuclear cells in rheumatoid arthritic patients

Rheumatology (Oxford). 2004 Aug;43(8):960-4.
"Long-term treatment with etanercept significantly reduces the number of proinflammatory cytokine-secreting peripheral blood mononuclear cells in patients with rheumatoid arthritis."
Author(s): Schotte H, Schlüter B, Willeke P, Mickholz E, Schorat MA, Domschke W, Gaubitz M
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
M303 was used in ELISA to study the effect of genotypes on the innate response against the candidate adjuvants RC529 and monophosphoryl lipid A

Vaccine. 2004 Mar 29;22(11-12):1515-23.
"Host factors impacting the innate response in humans to the candidate adjuvants RC529 and monophosphoryl lipid A."
Author(s): Tiberio L, Fletcher L, Eldridge JH, Duncan DD
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
M303 was used in ELISA to characterize the thalidomide as tumor necross factor-alpha inhibitors and its possible therapeutic effects on neurodegenerative diseases

Acta Neurobiol Exp (Wars). 2004;64(1):1-9.
"Thalidomide-based TNF-alpha inhibitors for neurodegenerative diseases."
Author(s): Greig NH, Giordano T, Zhu X, Yu QS, Perry TA, Holloway HW, Brossi A, Rogers JT, Sambamurti K, Lahiri DK
Number of Citations: 1
(See PubMed article )
  Hu / 300 ng/ml
M303 was used in ELISA to investigate the role of immunostimulant RU41740 from Klebsiella pneumoniae for innate and adaptive immune responses in human cells from whole blood

Int Immunopharmacol. 2006 Apr;6(4):635-46.
"The immunostimulant RU41740 from Klebsiella pneumoniae activates human cells in whole blood to potentially stimulate innate and adaptive immune responses."
Author(s): Pedraza-Sánchez S, González-Hernández Y, Escobar-Gutiérrez A, Ramachandra L
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
M303 was used in ELISA to study the role of sesquiterpene lactones in human monocyte-derived macrophage responses

Apoptosis. 2007 Jan;12(1):141-53.
"Sesquiterpene lactones induce distinct forms of cell death that modulate human monocyte-derived macrophage responses."
Author(s): López-Antón N, Hermann C, Murillo R, Merfort I, Wanner G, Vollmar AM, Dirsch VM
Number of Citations: 1
(See PubMed article )
  Hu / 1:5000
M303 was used in ELISA to investigate an immobilization method for efficient and highly sensitive sandwich ELISA system with smaller amounts of ligand antibodies

J Biotechnol. 2009 Jun 15;142(2):135-41.
"Efficient immobilization of a ligand antibody with high antigen-binding activity by use of a polystyrene-binding peptide and an intelligent microtiter plate."
Author(s): Kumada Y, Hamasaki K, Shiritani Y, Ohse T, Kishimoto M
Number of Citations: 0
(See PubMed article )
  Hu / Not Cited
M303 was used in ELISA to investigate the mechanism of human T cell regulation by anandamide

PLoS One. 2010 Jan 14;5(1):e8688.
"Anandamide suppresses proliferation and cytokine release from primary human T-lymphocytes mainly via CB2 receptors."
Author(s): Cencioni MT, Chiurchiù V, Catanzaro G, Borsellino G, Bernardi G, Battistini L, Maccarrone M
Number of Citations: 9
(See PubMed article )
  Hu / Not Cited
M303 was used in ELISA to study the temporal cytokine profiles in chronic fatigue syndrome with and without fibromyalgia during sleep

Clin Vaccine Immunol. 2010 Apr;17(4):582-7.
"Cytokines across the night in chronic fatigue syndrome with and without fibromyalgia."
Author(s): Nakamura T, Schwander SK, Donnelly R, Ortega F, Togo F, Broderick G, Yamamoto Y, Cherniack NS, Rapoport D, Natelson BH
Number of Citations: 1
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific