* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
PBOTNFAI targets TNF Alpha in ELISA, and WB applications and shows reactivity with Bovine samples.
The PBOTNFAI immunogen is tNF alpha.
PBOTNFAI detects TNF Alpha which has a predicted molecular weight of approximately 26 kDa.
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
PBOTNFAI was used in western blot to investigate the involvement of the NF-kB pathway and TNF-alpha in the cytotoxicity of anthrax lethal toxin
Vet Microbiol. 2010 Nov 20;146(1-2):111-7. "Activation of NF-?B pathway and TNF-? are involved in the cytotoxicity of anthrax lethal toxin in bovine BoMac macrophages."
Author(s): Liang X, Gao CF, Rutherford MS, Ji Y
Number of Citations: 0
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)