|Host / Isotype:||Rabbit / IgG|
|Species Reactivity:||Bovine (Bv)|
|Pierce TNF Alpha Antibody|
|Form:||1 mg of purified antibody by Protein G affinity chromatography and Lyophilized in preservative and carrier free PBS.|
|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
|Product Specific Information|
|PBOTNFAI targets TNF Alpha in ELISA, and WB applications and shows reactivity with Bovine samples.
The PBOTNFAI immunogen is tNF alpha.
PBOTNFAI detects TNF Alpha which has a predicted molecular weight of approximately 26 kDa.
|This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.|
|Species / Dilution||Summary|
Bv / Not Cited
PBOTNFAI was used in western blot to investigate the involvement of the NF-kB pathway and TNF-alpha in the cytotoxicity of anthrax lethal toxin
Vet Microbiol. 2010 Nov 20;146(1-2):111-7.
"Activation of NF-?B pathway and TNF-? are involved in the cytotoxicity of anthrax lethal toxin in bovine BoMac macrophages."
Author(s): Liang X, Gao CF, Rutherford MS, Ji Y
Number of Citations: 0
(See PubMed article )