| Details | |
| Host / Isotype: | Rabbit / IgG |
| Class: | Polyclonal |
| Type: | Antibody |
| Label: | Biotin |
| Species Reactivity: | Bovine (Bv) |
| Immunogen: | TNF alpha |
| Ordering Information | ||||
| Pierce TNF Alpha Antibody, Biotin conjugate |
| Storage: | 4° C |
| Form: | 250 ug of purified antibody by Protein G affinity chromatography and Lyophilized in preservative and carrier free PBS. |
| Applications | Dilution * |
| ELISA (ELISA) | Assay Dependent |
| Western Blot (WB) | Assay Dependent |
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* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
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| Product Specific Information |
| PBOTNFABI targets TNF Alpha in ELISA, and WB applications and shows reactivity with Bovine samples. The PBOTNFABI immunogen is tNF alpha. PBOTNFABI detects TNF Alpha which has a predicted molecular weight of approximately 26 kDa. The PBOTNFABI polyclonal biotinylated bovine TNFa antibody has successfully been paired as the detection antibody in a sandwich ELISA with monoclonal capture antibody MBOTNFAI (clone E10011). Typical dilutions for sandwich ELISA include 1 µg/ml for coating and 0.125 - 0.25 µg/ml for detection. |
| General Information |
| This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. |