* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
PBOTNFABI targets TNF Alpha in ELISA, and WB applications and shows reactivity with Bovine samples.
The PBOTNFABI immunogen is tNF alpha.
PBOTNFABI detects TNF Alpha which has a predicted molecular weight of approximately 26 kDa.
The PBOTNFABI polyclonal biotinylated bovine TNFa antibody has successfully been paired as the detection antibody in a sandwich ELISA with monoclonal capture antibody MBOTNFAI (clone E10011). Typical dilutions for sandwich ELISA include 1 µg/ml for coating and 0.125 - 0.25 µg/ml for detection.
This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)