| Details | |
| Host / Isotype: | Rabbit / IgG |
| Class: | Polyclonal |
| Type: | Antibody |
| Species Reactivity: | Xenopus laevis (Xl) Human (Hu) Mouse (Ms) Rat (Rt) |
| Immunogen: | synthetic peptide DDKKHHDAEHHRDREPGT conjugated to BSA, corresponding to amino acids 181-198 of Human TLE1. |
| Ordering Information | ||||
| Pierce TLE1 Antibody |
| Storage: | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Form: | 50 ug of immunoaffinity purified antibody (1 mg/ml) in PBS, pH 7.2, with 50% glycerol and 0.09% sodium azide. |
| Applications | Dilution * |
| Western Blot (WB) | 1:200 - 1:1000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 - 1:1000 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:200 - 1:1000 |
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* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
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| Product Specific Information |
| PA1-28063 detects TLE1 from mouse, human, Xenopus laevis and rat samples. Expected to cross-react with chicken (92% identity with immunogen) due to sequence homology. PA1-28063 has been successfully used in immunohistochemistry (frozen tissue), immunohistochemistry (paraffin tissue), and Western blot applications. By Western Blot a band of ~83.5 kDa is detected. The PA1-28063 immunogen is Synthetic peptide: DDKKHHDAEHHRDREPGT conjugated to BSA, corresponding to amino acids 181-198 of Human TLE1. Store at 4ºC short term. For extended storage, store at -20ºC, avoiding freeze/thaw cycles. |
| General Information |
| Groucho (TLE1) (Transduction-like enhancer protein 1, ESG1) is a member of the Groucho family of transcriptional corepressors and is involved in oligomerization, association with chromatin and interaction with histone H3. |