TCR V alpha 12.1 Antibody (6D6.6)

TCR V alpha 12.1 Monoclonal Antibody for Western blot, IHC (F), FACS, IP, TCA

>> See 9 other antibodies for TRAC
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Mouse / IgG1
Class: Monoclonal
Type: Antibody
Clone: 6D6.6
Tested Species Reactivity: Human (Hu), Rat (Rt)
Published Species Reactivity: Human (Hu), Mouse (Ms)
Immunogen: Human TCR V alpha12.1
Ordering Information
Pierce TCR V alpha 12.1 Antibody (6D6.6)
Product #
TCR1764
Size
200 ug
Price
$316.00
Purchase
Add TCR V alpha 12.1 Antibody (6D6.6) to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:1-1:100
Immunohistochemistry (Frozen) (IHC (F)) Assay Dependent
Flow Cytometry (FACS) 2 ug/test
Immunoprecipitation (IP) Assay Dependent
T-Cell Activation (TCA) Assay Dependent
Published Applications Dilution
Immunohistochemistry (IHC) See publications below
Flow Cytometry (FACS) See publications below
Immunoprecipitation (IP) See publications below
Blocking Assay (BLOCK) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 0.15mg/ml
Purification: Protein G
Storage Buffer: PBS with 0.5% BSA
Preservative: 0.1% sodium azide
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
TCR1764 targets TCR V alpha 12.1 in FACS, IHC (F), IP, WB and TCA applications and shows reactivity with Human and Rat samples.

The TCR1764 immunogen is human TCR V alpha12.1.
General Information
T Cell Receptors - The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response. TCR recognition of self-peptides has been linked to autoimmune disease. Mutant self-peptides have been associated with tumors. Engagement of TCRs by a family of bacterial toxins know as superantigens has been responsible for toxic shock syndrome (ref2). Autoantibodies to V beta segments of T cell receptors have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). The autoantibodies block TH1-mediated inflammatory autodestructive reactions and are believed to be a method by which the immune system compensates for disease (ref5).
T Cell and TCR Diversity Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP). A small number of T cells lack both CD4 and CD8 (double negative, DN). Increased percentages of alpha-beta DN T cells have been identified in some autoimmune and immunodeficiency disorders (ref3). Gamma-delta T cells are primarily found within the epithelium. They show less TCR diversity and recognize antigens differently than alpha-beta T cells. Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity (refs1,4).
TCR-specific Antibodies - The Thermo Scientific T cell receptor offering includes pan alphabeta and pan gammadelta antibodies as well as V alpha-, V beta-, V delta-, V gamma-, Beta F1-, Alpha F1-, and C gamma-specific antibodies. All antibodies have been QC-tested for use in flow cytometry and/or immunohistochemistry. Most clones are available in purified and FITC-labeled formats.
Product Images
  • TCR V alpha 12.1 Antibody (TCR1764) in WB
    TCR1764_WB.jpg

    Western Blot with anti-TCR V alpha 12.1 Monoclonal Antibody [6D6.6] (TCR1764) (TCR1764_WB.jpg)

    Western Blot with anti-TCR V alpha 12.1 Monoclonal Antibody [6D6.6] (TCR1764)

    Western blot analysis of TCR V alpha 12.1 was performed by loading 25 ug of Jurkat (lane 1), CEM (lane 2) and rat thymus (lane 3) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a TCR V alpha 12.1 monoclonal antibody (Product # TCR1764) at a dilution of 1:10 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~30-35 kDa.
  • TCR V alpha 12.1 Antibody (TCR1764) in FACS
    TCR1764_FACS_Jurkat_20130801083016.jpg

    Flow Cytometry with anti-TCR V alpha 12.1 Monoclonal Antibody [6D6.6] (TCR1764) (TCR1764_FACS_Jurkat_20130801083016.jpg)

    Flow Cytometry with anti-TCR V alpha 12.1 Monoclonal Antibody [6D6.6] (TCR1764)

    Flow cytometry analysis of TCR V alpha 12.1 in Jurkat cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a TCR V alpha 12.1 monoclonal antibody (Product # TCR1764) at a dilution of 2 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.
  • TCR V alpha 12.1 Antibody (TCR1764) in FACS
    TCR1764_FACS_PBMC_20130801083034.jpg

    Flow Cytometry with anti-TCR V alpha 12.1 Monoclonal Antibody [6D6.6] (TCR1764) (TCR1764_FACS_PBMC_20130801083034.jpg)

    Flow Cytometry with anti-TCR V alpha 12.1 Monoclonal Antibody [6D6.6] (TCR1764)

    Flow cytometry analysis of TCR V alpha 12.1 in PBMC cells (green) compared to an isotype control (blue). Human blood was collected, combined with a hydrophilic polysaccharide, centrifuged, transferred to a conical tube and washed with PBS. 50 ul of cell solution was added to each tube at a dilution of 2x10^7 cells/ml, followed by the addition of 50 ul of isotype control and primary antibody (Product # TCR1764) at a dilution of 2 ug/test. Cells were incubated for 30 min at 4ºC and washed with a cell buffer, followed by incubation with a DyLight 488-conjugated goat anti-mouse IgG (H+L) secondary for 30 min at 4ºC in the dark. FACS analysis was performed using 400 ul of cell buffer.
  • TCR V alpha 12.1 Antibody (TCR1764) in FACS
    TCR1764_FACS_PBMC_scatter_20130801083051.jpg

    Flow Cytometry with anti-TCR V alpha 12.1 Monoclonal Antibody [6D6.6] (TCR1764) (TCR1764_FACS_PBMC_scatter_20130801083051.jpg)

    Flow Cytometry with anti-TCR V alpha 12.1 Monoclonal Antibody [6D6.6] (TCR1764)

    Flow cytometry analysis of TCR V alpha 12.1 in PBMC cells (green) compared to an isotype control (blue). Human blood was collected, combined with a hydrophilic polysaccharide, centrifuged, transferred to a conical tube and washed with PBS. 50 ul of cell solution was added to each tube at a dilution of 2x10^7 cells/ml, followed by the addition of 50 ul of isotype control and primary antibody (Product # TCR1764) at a dilution of 2 ug/test. Cells were incubated for 30 min at 4ºC and washed with a cell buffer, followed by incubation with a DyLight 488-conjugated goat anti-mouse IgG (H+L) secondary for 30 min at 4ºC in the dark. FACS analysis was performed using 400 ul of cell buffer.
Publications:
Immunohistochemistry
  Species / Dilution Summary
  Hu / Not Cited
TCR1764 was used in immunohistochemistry to investigate the increase of V alpha 12.1+, CD8+ T cells in rheumatoid arthritis patients

J Exp Med. 1993 Jun 1;177(6):1623-31.
"Clonal V alpha 12.1+ T cell expansions in the peripheral blood of rheumatoid arthritis patients."
Author(s): DerSimonian H, Sugita M, Glass DN, Maier AL, Weinblatt ME, Rème T, Brenner MB
Number of Citations: 20
(See PubMed article )
Flow Cytometry
  Species / Dilution Summary
  Hu / Not Cited
TCR1764 was used in blocking/activating experiment, flow cytometry and immunoprecipitation to investigate the distribution and role of TCR V alpha 12.1 in CD8 T cells

J Exp Med. 1991 Sep 1;174(3):639-48.
"Increased frequency of T cell receptor V alpha 12.1 expression on CD8+ T cells: evidence that V alpha participates in shaping the peripheral T cell repertoire."
Author(s): DerSimonian H, Band H, Brenner MB
Number of Citations: 19
(See PubMed article )
  Hu / Not Cited
TCR1764 was used in flow cytometry to identify a specific peptide from human carcinoembryonic antigen to enhance the immunogenicity of tumor cell self-antigens

Cancer Res. 1997 Oct 15;57(20):4570-7.
"Identification of an enhancer agonist cytotoxic T lymphocyte peptide from human carcinoembryonic antigen."
Author(s): Zaremba S, Barzaga E, Zhu M, Soares N, Tsang KY, Schlom J
Number of Citations: 16
(See PubMed article )
  Hu / Not Cited
TCR1764 was used in flow cytometry to characterize the CD4+CD25high Foxp3+ regulatory T cell repertoire

J Immunol. 2007 Sep 15;179(6):3412-6.
"Cutting edge: size and diversity of CD4+CD25high Foxp3+ regulatory T cell repertoire in humans: evidence for similarities and partial overlapping with CD4+CD25- T cells."
Author(s): Fazilleau N, Bachelez H, Gougeon ML, Viguier M
Number of Citations: 7
(See PubMed article )
  Ms / Not Cited
TCR1764 was used in flow cytometry to study the role of autoreactive human T-cell receptor in insulitis and impairement of glucose tolerance

J Autoimmun. 2008 Jun;30(4):197-206.
"Autoreactive human T-cell receptor initiates insulitis and impaired glucose tolerance in HLA DR4 transgenic mice."
Author(s): Gebe JA, Unrath KA, Yue BB, Miyake T, Falk BA, Nepom GT
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
TCR1764 was used in flow cytometry to investigate the mechanism for autoreactive T cells in autoimmune diabetes

J Immunol. 2009 Jul 1;183(1):59-65.
"Restricted autoantigen recognition associated with deletional and adaptive regulatory mechanisms."
Author(s): Gebe JA, Yue BB, Unrath KA, Falk BA, Nepom GT
Number of Citations: 1
(See PubMed article )
Immunoprecipitation
  Species / Dilution Summary
  Hu / Not Cited
TCR1764 was used in blocking/activating experiment, flow cytometry and immunoprecipitation to investigate the distribution and role of TCR V alpha 12.1 in CD8 T cells

J Exp Med. 1991 Sep 1;174(3):639-48.
"Increased frequency of T cell receptor V alpha 12.1 expression on CD8+ T cells: evidence that V alpha participates in shaping the peripheral T cell repertoire."
Author(s): DerSimonian H, Band H, Brenner MB
Number of Citations: 19
(See PubMed article )
Blocking Assay
  Species / Dilution Summary
  Hu / 50 ng/ml
TCR1764 was used in blocking/activating experiment, flow cytometry and immunoprecipitation to investigate the distribution and role of TCR V alpha 12.1 in CD8 T cells

J Exp Med. 1991 Sep 1;174(3):639-48.
"Increased frequency of T cell receptor V alpha 12.1 expression on CD8+ T cells: evidence that V alpha participates in shaping the peripheral T cell repertoire."
Author(s): DerSimonian H, Band H, Brenner MB
Number of Citations: 19
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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