TCR Alpha F1 Antibody (3A8)

TCR Alpha F1 Monoclonal Antibody for IHC (F), IHC (P), IP

>> See 9 other antibodies for TRA (T-cell antigen receptor, alpha polypeptide; T-cell receptor, alpha (V,D,J,C))
Synonyms:
T-cell antigen receptor, alpha polypeptide; T-cell receptor, alpha (V,D,J,C)
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Mouse / IgG2a
Class: Monoclonal
Type: Antibody
Clone: 3A8
Tested Species Reactivity: Human (Hu)
Published Species Reactivity: Human (Hu), Yeast (Ys)
Immunogen: Human TCR ? chain constant region
Ordering Information
Pierce TCR Alpha F1 Antibody (3A8)
Product #
TCR1145
Size
200 ug
Price
$299.00
Purchase
Add TCR Alpha F1 Antibody (3A8) to your cart.
Tested Applications Dilution *
Immunohistochemistry (Frozen) (IHC (F)) Assay Dependent
Immunohistochemistry (Paraffin) (IHC (P)) 1:10-1:100
Immunoprecipitation (IP) Assay Dependent
Published Applications Dilution
Western Blot (WB) See publications below
Immunohistochemistry (IHC) See publications below
Flow Cytometry (FACS) See publications below
Immunoprecipitation (IP) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 0.15mg/ml
Purification: Protein A
Storage Buffer: PBS with 0.5% BSA
Preservative: 0.1% sodium azide
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
TCR1145 targets TCR Alpha F1 in IHC (F), IHC (P) and IP applications and shows reactivity with Human samples.

The TCR1145 immunogen is human TCR a chain constant region.
General Information
T Cell Receptors - The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response. TCR recognition of self-peptides has been linked to autoimmune disease. Mutant self-peptides have been associated with tumors. Engagement of TCRs by a family of bacterial toxins know as superantigens has been responsible for toxic shock syndrome (ref2). Autoantibodies to V beta segments of T cell receptors have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). The autoantibodies block TH1-mediated inflammatory autodestructive reactions and are believed to be a method by which the immune system compensates for disease (ref5).
T Cell and TCR Diversity Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP). A small number of T cells lack both CD4 and CD8 (double negative, DN). Increased percentages of alpha-beta DN T cells have been identified in some autoimmune and immunodeficiency disorders (ref3). Gamma-delta T cells are primarily found within the epithelium. They show less TCR diversity and recognize antigens differently than alpha-beta T cells. Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity (refs1,4).
TCR-specific Antibodies - The Thermo Scientific T cell receptor offering includes pan alphabeta and pan gammadelta antibodies as well as V alpha-, V beta-, V delta-, V gamma-, Beta F1-, Alpha F1-, and C gamma-specific antibodies. All antibodies have been QC-tested for use in flow cytometry and/or immunohistochemistry. Most clones are available in purified and FITC-labeled formats.
Product Images
  • TCR Alpha F1 Antibody (TCR1145) in IHC (P)
    TCR1145_IHC_human_tonsil_tissue_20130801081813.jpg

    Immunohistochemistry (Paraffin) with anti-TCR Alpha F1 Monoclonal Antibody [3A8] (TCR1145) (TCR1145_IHC_human_tonsil_tissue_20130801081813.jpg)

    Immunohistochemistry (Paraffin) with anti-TCR Alpha F1 Monoclonal Antibody [3A8] (TCR1145)

    Immunohistochemistry analysis of TCR Alpha F1 showing staining in the cytoplasm of paraffin-treated human tonsil tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a TCR Alpha F1 monoclonal antibody (Product # TCR1145) diluted by 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
  • TCR Alpha F1 Antibody (TCR1145) in IHC (P)
    TCR1145_IHC_human_colon_tissue_20130801081838.jpg

    Immunohistochemistry (Paraffin) with anti-TCR Alpha F1 Monoclonal Antibody [3A8] (TCR1145) (TCR1145_IHC_human_colon_tissue_20130801081838.jpg)

    Immunohistochemistry (Paraffin) with anti-TCR Alpha F1 Monoclonal Antibody [3A8] (TCR1145)

    Immunohistochemistry analysis of TCR Alpha F1 showing staining in the cytoplasm of paraffin-treated human colon tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a TCR Alpha F1 monoclonal antibody (Product # TCR1145) diluted by 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
Publications:
Western Blot
  Species / Dilution Summary
  Hu / Not Cited
TCR1145 was used in western blot to investigate the role of HLA-DR2 for T-cell activation

J Biol Chem. 2000 Jan 7;275(1):312-21.
"Kinetics of T-cell receptor binding by bivalent HLA-DR. Peptide complexes that activate antigen-specific human T-cells."
Author(s): Appel H, Gauthier L, Pyrdol J, Wucherpfennig KW
Number of Citations: 10
(See PubMed article )
  Hu / 0
TCR1145 was used in flow cytometry, immunohistochemistry, and western blot to investigate the effect of macrophage-TCRalpha beta on granuloma development in tuberculosis

PLoS Pathog. 2011 Nov;7(11):e1002375.
"A TNF-regulated recombinatorial macrophage immune receptor implicated in granuloma formation in tuberculosis."
Author(s): Beham AW, Puellmann K, Laird R, Fuchs T, Streich R, Breysach C, Raddatz D, Oniga S, Peccerella T, Findeisen P, Kzhyshkowska J, Gratchev A, Schweyer S, Saunders B, Wessels JT, Möbius W, Keane J, Becker H, Ganser A, Neumaier M, Kaminski WE
Number of Citations: 1
(See PubMed article )
Immunohistochemistry
  Species / Dilution Summary
  Hu / Not Cited
TCR1145 was used in immunohistochemistry to study the effect of CD3 surface expression on cutaneous T cell lymphoma cells

Clin Exp Immunol. 1998 Aug;113(2):190-7.
"Immunological study on CD3 defective cutaneous T cell lymphoma cells from a patient with Sézary syndrome."
Author(s): Sano S, Matsui Y, Itami S, Yoshikawa K
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
TCR1145 was used in immunohistochemistry to investigate the activation of cytotoxic T cells in duodenal epithelium from patients with florid coeliac disease

J Pathol. 2005 Jun;206(2):178-85.
"Cytotoxic T cells are preferentially activated in the duodenal epithelium from patients with florid coeliac disease."
Author(s): Buri C, Burri P, Bähler P, Straumann A, Müller-Schenker B, Birrer S, Mueller C
Number of Citations: 1
(See PubMed article )
  Hu / 1:100
TCR1145 was used in flow cytometry, immunohistochemistry, and western blot to investigate the effect of macrophage-TCRalpha beta on granuloma development in tuberculosis

PLoS Pathog. 2011 Nov;7(11):e1002375.
"A TNF-regulated recombinatorial macrophage immune receptor implicated in granuloma formation in tuberculosis."
Author(s): Beham AW, Puellmann K, Laird R, Fuchs T, Streich R, Breysach C, Raddatz D, Oniga S, Peccerella T, Findeisen P, Kzhyshkowska J, Gratchev A, Schweyer S, Saunders B, Wessels JT, Möbius W, Keane J, Becker H, Ganser A, Neumaier M, Kaminski WE
Number of Citations: 1
(See PubMed article )
Flow Cytometry
  Species / Dilution Summary
  Hu / Not Cited
TCR1145 was used in flow cytometry to study the regulatory mechanism for CD8+ T lymphocyte cytolytic activity

Eur J Immunol. 2002 Jun;32(6):1688-97.
"A reversible functional defect of CD8+ T lymphocytes involving loss of tetramer labeling."
Author(s): Demotte N, Colau D, Ottaviani S, Godelaine D, Van Pel A, Boon T, van der Bruggen P
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
TCR1145 was used in flow cytometry to optimize the yeast surface display of TCRs and the scFv D1.3

Mol Immunol. 2009 Feb;46(5):902-16.
"Structural features of T cell receptor variable regions that enhance domain stability and enable expression as single-chain ValphaVbeta fragments."
Author(s): Richman SA, Aggen DH, Dossett ML, Donermeyer DL, Allen PM, Greenberg PD, Kranz DM
Number of Citations: 1
(See PubMed article )
  Hu / 0
TCR1145 was used in flow cytometry, immunohistochemistry, and western blot to investigate the effect of macrophage-TCRalpha beta on granuloma development in tuberculosis

PLoS Pathog. 2011 Nov;7(11):e1002375.
"A TNF-regulated recombinatorial macrophage immune receptor implicated in granuloma formation in tuberculosis."
Author(s): Beham AW, Puellmann K, Laird R, Fuchs T, Streich R, Breysach C, Raddatz D, Oniga S, Peccerella T, Findeisen P, Kzhyshkowska J, Gratchev A, Schweyer S, Saunders B, Wessels JT, Möbius W, Keane J, Becker H, Ganser A, Neumaier M, Kaminski WE
Number of Citations: 1
(See PubMed article )
  Ys / Not Cited
TCR1145 was used in flow cytometry to development a novel approach to stably express single-chain T cell receptors

Protein Eng Des Sel. 2011 Apr;24(4):361-72.
"Identification and engineering of human variable regions that allow expression of stable single-chain T cell receptors."
Author(s): Aggen DH, Chervin AS, Insaidoo FK, Piepenbrink KH, Baker BM, Kranz DM
Number of Citations: 1
(See PubMed article )
Immunoprecipitation
  Species / Dilution Summary
  Hu / Not Cited
TCR1145 was used in immunoprecipitation to study the mechanism for internalization and degradation of the pre-TCR molecules

J Biol Chem. 2003 Apr 18;278(16):14507-13.
"A role for the cytoplasmic tail of the pre-T cell receptor (TCR) alpha chain in promoting constitutive internalization and degradation of the pre-TCR."
Author(s): Carrasco YR, Navarro MN, Toribio ML
Number of Citations: 1
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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