TARDBP Antibody

TARDBP Polyclonal Antibody for Western blot, IF, ICC, IHC (P), IP, ChIP

>> See 8 other antibodies for TARDBP (ALS10, TDP-43, TAR DNA-binding protein 43, TAR DNA-binding protein-43)
Synonyms:
ALS10, TDP-43, TAR DNA-binding protein 43, TAR DNA-binding protein-43
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Rabbit / IgG
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu), Mouse (Ms), Rat (Rt)
Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 289 of Human TARDBP
Ordering Information
Pierce TARDBP Antibody
Product #
PA5-29949
Size
100 ul
Price
$316.00
Purchase
Add TARDBP Antibody to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:500-1:3000
Immunofluorescence (IF) 1:100-1:1000
Immunocytochemistry (ICC) 1:100-1:1000
Immunohistochemistry (Paraffin) (IHC (P)) 1:100-1:1000
Immunoprecipitation (IP) 1:100-1:1000
ChIP assay (ChIP) Assay-dependent
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 1.01mg/ml
Purification: Antigen affinity chromatography
Storage Buffer: 0.1M tris, pH 7, with 20% glycerol, 0.1M glycine
Preservative: 0.01% thimerosal
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
PA5-29949 targets TARDBP in IF, IHC (P), and WB applications and shows reactivity with Human and Rat samples.

The PA5-29949 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 289 of Human TARDBP.
General Information
HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20.
Product Images
  • TARDBP Antibody (PA5-29949) in WB
    TARDBP_PA5-29949_Antibody_WesternBlot.jpg

    Western Blot with anti-TARDBP Polyclonal Antibody (PA5-29949) (TARDBP_PA5-29949_Antibody_WesternBlot.jpg)

    Western Blot with anti-TARDBP Polyclonal Antibody (PA5-29949)

    Western Blot analysis using anti-TARDBP Polyclonal Antibody on the following cells: (30 ug of whole cell lysate) A: A431 cell lysate B: H1299 C: Hela. Primary antibody (PA5-29949) diluted at 1:1000
  • TARDBP Antibody (PA5-29949) in WB
    TARDBP_PA5-29949_Antibody_WesternBlot_2.jpg

    Western Blot with anti-TARDBP Polyclonal Antibody (PA5-29949) (TARDBP_PA5-29949_Antibody_WesternBlot_2.jpg)

    Western Blot with anti-TARDBP Polyclonal Antibody (PA5-29949)

    Western blot analysis of TDP-43 (PA5-29949 upper panel) and beta-actin (lower panel)  Sample (30 ug of whole cell lysate)   A: HeLa mock control  B: HeLa transfected shTDP-43. Primary antibodies (PA5-29949) diluted at 1:1000
  • TARDBP Antibody (PA5-29949) in IF
    TARDBP_PA5-29949_Antibody_Immunofluorescence.jpg

    Immunofluorescence with anti-TARDBP Polyclonal Antibody (PA5-29949) (TARDBP_PA5-29949_Antibody_Immunofluorescence.jpg)

    Immunofluorescence with anti-TARDBP Polyclonal Antibody (PA5-29949)

    Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells using TDP-43 (PA5-29949) antibody at 1:200 dilution.
  • TARDBP Antibody (PA5-29949) in IHC
    TARDBP_PA5-29949_Antibody_Immunohistochemistry.jpg

    Immunohistochemistry with anti-TARDBP Polyclonal Antibody (PA5-29949) (TARDBP_PA5-29949_Antibody_Immunohistochemistry.jpg)

    Immunohistochemistry with anti-TARDBP Polyclonal Antibody (PA5-29949)

    Immunohistochemical analysis of paraffin-embedded rat brain using TARDBP (PA5-29949) antibody at 1:500 dilution.
  • TARDBP Antibody (PA5-29949) in IHC
    TARDBP_PA5-29949_Antibody_Immunohistochemistry_2.jpg

    Immunohistochemistry with anti-TARDBP Polyclonal Antibody (PA5-29949) (TARDBP_PA5-29949_Antibody_Immunohistochemistry_2.jpg)

    Immunohistochemistry with anti-TARDBP Polyclonal Antibody (PA5-29949)

    Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft using TDP-43 (PA5-29949) antibody at 1:100 dilution.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific