TARDBP Antibody

TARDBP Polyclonal Antibody for Western blot, IF, ICC, IHC (P), IP, ChIP

>> See 10 other antibodies for TARDBP (ALS10, TDP-43, TAR DNA-binding protein 43, TAR DNA-binding protein-43)
Synonyms:
ALS10, TDP-43, TAR DNA-binding protein 43, TAR DNA-binding protein-43
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Rabbit / IgG
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu), Mouse (Ms), Rat (Rt)
Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 289 of Human TARDBP
Ordering Information
Pierce TARDBP Antibody
Product #
PA5-29949
Size
100 µL
Price
$324.00
Purchase
Tested Applications Dilution *
Western Blot (WB) 1:500-1:3000
Immunofluorescence (IF) 1:100-1:1000
Immunocytochemistry (ICC) 1:100-1:1000
Immunohistochemistry (Paraffin) (IHC (P)) 1:100-1:1000
Immunoprecipitation (IP) 1:100-1:1000
ChIP assay (ChIP) Assay-dependent
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 1.01mg/ml
Purification: Antigen affinity chromatography
Storage Buffer: 0.1M tris, pH 7, with 20% glycerol, 0.1M glycine
Preservative: 0.01% thimerosal
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
PA5-29949 targets TARDBP in IF, IHC (P), and WB applications and shows reactivity with Human and Rat samples.

The PA5-29949 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 289 of Human TARDBP.
General Information
HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20.
Product Images
  • TARDBP Antibody (PA5-29949) in WB
    PA5-29949_WB_1.jpg

    Western Blot with anti-TARDBP Polyclonal Antibody (PA5-29949) (PA5-29949_WB_1.jpg)

    Western Blot with anti-TARDBP Polyclonal Antibody (PA5-29949)

    Western blot analysis of TARDBP using A) 30 µg Neuro2A whole cell lysate (B) 30 ug GL261 whole cell lysate (C) 30 ug NIH-3T3 whole cell lysate (D) 30 ug BCL-1 whole cell lysate and E) 30 ug Raw264.7 whole cell lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a TARDBP polyclonal antibody (Product # PA5-29949) at a dilution of 1:3000.
  • TARDBP Antibody (PA5-29949) in WB
    PA5-29949_WB_2.jpg

    Western Blot with anti-TARDBP Polyclonal Antibody (PA5-29949) (PA5-29949_WB_2.jpg)

    Western Blot with anti-TARDBP Polyclonal Antibody (PA5-29949)

    Western blot analysis of TARDBP using 50µg of rat brain lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a TARDBP polyclonal antibody (Product # PA5-29949) at a dilution of 1:3000.
  • TARDBP Antibody (PA5-29949) in WB
    PA5-29949_WB_3.jpg

    Western Blot with anti-TARDBP Polyclonal Antibody (PA5-29949) (PA5-29949_WB_3.jpg)

    Western Blot with anti-TARDBP Polyclonal Antibody (PA5-29949)

    Western blot analysis of TARDBP using 30µg of A) A431 (B) H1299 and C) HeLa lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a TARDBP polyclonal antibody (Product # PA5-29949) at a dilution of 1:1000.
  • TARDBP Antibody (PA5-29949) in IF
    PA5-29949_IF.jpg

    Immunofluorescence with anti-TARDBP Polyclonal Antibody (PA5-29949) (PA5-29949_IF.jpg)

    Immunofluorescence with anti-TARDBP Polyclonal Antibody (PA5-29949)

    Immunofluorescent analysis of TARDBP in paraformaldehyde-fixed HeLa cells using a TARDBP polyclonal antibody (Product # PA5-29949) at a 1:200 dilution.
  • TARDBP Antibody (PA5-29949) in IHC (P)
    PA5-29949_IHC_2.jpg

    Immunohistochemistry (Paraffin) with anti-TARDBP Polyclonal Antibody (PA5-29949) (PA5-29949_IHC_2.jpg)

    Immunohistochemistry (Paraffin) with anti-TARDBP Polyclonal Antibody (PA5-29949)

    Immunohistochemical analysis of TARDBP in paraffin-embedded rat brain tissue using a TARDBP polyclonal antibody (Product # PA5-29949) at a dilution of 1:500.
  • TARDBP Antibody (PA5-29949) in IHC (P)
    PA5-29949_IHC_1.jpg

    Immunohistochemistry (Paraffin) with anti-TARDBP Polyclonal Antibody (PA5-29949) (PA5-29949_IHC_1.jpg)

    Immunohistochemistry (Paraffin) with anti-TARDBP Polyclonal Antibody (PA5-29949)

    Immunohistochemical analysis of TARDBP in paraffin-embedded Cal27 xenograft using a TARDBP polyclonal antibody (Product # PA5-29949) at a dilution of 1:100.
  • TARDBP Antibody (PA5-29949) in IP
    PA5-29949_IP.jpg

    Immunoprecipitation with anti-TARDBP Polyclonal Antibody (PA5-29949) (PA5-29949_IP.jpg)

    Immunoprecipitation with anti-TARDBP Polyclonal Antibody (PA5-29949)

    PA5-29949 immunoprecipitates TARDBP protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 ?g HeLa whole cell lysate/extract B. Control with 2 ?g of preimmune rabbit IgG C. Immunoprecipitation of TARDBP protein by 2 ?g of TARDBP antibody (Product # PA5-29949) on 12% SDS-PAGE.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific