* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Antigen affinity chromatography
0.1M tris, pH 7,
20% glycerol, 0.1M glycine
-20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
PA5-29949 targets TARDBP in IF, IHC (P), and WB applications and shows reactivity with Human and Rat samples.
The PA5-29949 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 289 of Human TARDBP.
HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20.
TARDBP Antibody (PA5-29949) in WB TARDBP_PA5-29949_Antibody_WesternBlot.jpg
Western Blot with anti-TARDBP Polyclonal Antibody (PA5-29949)
Western blot analysis of TDP-43 (PA5-29949 upper panel) and beta-actin (lower panel) Sample (30 ug of whole cell lysate) A: HeLa mock control B: HeLa transfected shTDP-43. Primary antibodies (PA5-29949) diluted at 1:1000
TARDBP Antibody (PA5-29949) in IF TARDBP_PA5-29949_Antibody_Immunofluorescence.jpg
Immunofluorescence with anti-TARDBP Polyclonal Antibody (PA5-29949)