TARDBP Antibody

TARDBP Polyclonal Antibody for Western blot, IF, ICC, IHC

>> See 8 other antibodies for TARDBP (TARD binding protein , TAR DNA-binding protein 43 , TDP-43 , TDP43 )
Synonyms:
TARD binding protein , TAR DNA-binding protein 43 , TDP-43 , TDP43
Details
Host / Isotype: Rabbit
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu), Mouse (Ms), Chicken (Ck), Non-human primate (Nhp), Xenopus laevis (Xl), Zebrafish (Zf)
Immunogen: Synthetic peptide corresponding to C terminal region within residues 350-414 of the human TARDBP protein.
Ordering Information
Pierce TARDBP Antibody
Product #
PA1-16996
Size
100 µl
Price
$330.00
Purchase
Add TARDBP Antibody to your cart.
Tested Applications Dilution *
Western Blot (WB) 0.5-2 µg/ml
Immunofluorescence (IF) 1:100-1:1000
Immunocytochemistry (ICC) 1:100-1:1000
Immunohistochemistry (IHC) Assay-Dependent
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 1mg/ml
Purification: Antigen affinity chromatography
Storage Buffer: TBS
Preservative: 0.05% sodium azide
Storage Conditions: 4° C, do not freeze
Product Specific Information
Suggested positive control: Hela whole cell extract, antigen standard for TARDBP (transient overexpression lysate).
General Information
The HIV1 virus gives rise to acquired immunodeficiency syndrome (AIDS). HIV1 contains an RNA genome which produces chromosomally integrated DNA during cellular replication. Gene activation of HIV1 by the transactivator Tat requires an RNA regulatory element TAR which is located downstream of the transcription initiation site. TARDBP is a transcriptional repressor that binds to the chromosomally integrated TAR DNA, preventing HIV1 expression. Additionally, this protein regulates alternative splicing of the CTFR gene, a similar pseudogene on chromosoome 20. TARDBP has also been recently been shown to abnormally accumulate in the post-mortem brain tissue of individuals diagnosed with ALS (Amyotrophic Lateral Sclerosis) and FTD (Frontal Temporal Dementia). This long-awaited conncection between these two diseases provides an explanation for the observed clinical overlap.
Product Images
  • TARDBP Antibody (PA1-16996) in WB
    PA1-16996_Westernblot.jpg

    Western Blot with anti-TARDBP Polyclonal Antibody (PA1-16996) (PA1-16996_Westernblot.jpg)

    Western Blot with anti-TARDBP Polyclonal Antibody (PA1-16996)

    cells were transfected with the pCMV6-ENTRY control or pCMV6-ENTRY TARDBP cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TARDBP.
  • TARDBP Antibody (PA1-16996) in WB
    PA1-16996_Westernblot2.jpg

    Western Blot with anti-TARDBP Polyclonal Antibody (PA1-16996) (PA1-16996_Westernblot2.jpg)

    Western Blot with anti-TARDBP Polyclonal Antibody (PA1-16996)

    Western Blot analysis of in HeLa WCE using PA1-16996.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific