TAMRA Antibody (5G5)

TAMRA Monoclonal Antibody for IP, Western blot

Details
Host / Isotype: Mouse / IgG
Class: Monoclonal
Type: Antibody
Clone: 5G5
Species Reactivity: Chemical (Chem) Tag (Tag)
Immunogen: TAMRA molecule
Ordering Information
Pierce TAMRA Antibody (5G5)
Product #
MA1-041
Size
100 µg
Price
$299.00
Purchase
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Storage: -20°C
Form: 100 µg of protein G purified antibody (1 mg/ml) in PBS, 1 mg/ml BSA, and 0.05% sodium azide.


Applications Dilution *
Immunoprecipitation (IP) 10 µg
Western Blot (WB) 1:1000
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
MA1-041 detects TAMRA in samples and has been used in western blotting and immunoprecipitation applications.
General Information
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  • TAMRA Antibody (MA1-041) in IP
    Immunoprecipitation with anti-TAMRA Monoclonal Antibody [5G5] (MA1-041)

    Immunoprecipitation with anti-TAMRA Monoclonal Antibody [5G5] (MA1-041)

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    Immunoprecipitation was performed on purified TAMRA-BSA. The antigen:antibody complex was formed by binding 5µg protein with 10µg of mouse monoclonal antibody recognizing TAMRA (Product # MA1-041) or Mouse IgG, whole molecule ( Product # 31202) overnight on a rocking platform at 4°C. The immune-complex was then captured on 50µl Protein A/G Plus Agarose (Product #20423) . Captured immune-complexes were then washed extensively and proteins eluted with 5X Reducing Sample Loading Dye (Product # 39000). Samples were then resolved on a 4-20% Tris-HCl polyacrylamide gel. Proteins were imaged using Typhoon 9410 Imager at 532nM , Normal Sensitivity.
     
  • TAMRA Antibody (MA1-041) in WB
    Western Blot with anti-TAMRA Monoclonal Antibody [5G5] (MA1-041)

    Western Blot with anti-TAMRA Monoclonal Antibody [5G5] (MA1-041)

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    Western blot analysis was performed by loading various amounts of purified TAMRA-BSA protein onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were then probed with a mouse monoclonal antibody recognizing TAMRA (Product #MA1-041) at a dilution of 1:1000 for 1hour at room temperature on a rocking platform. Membranes were then washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody (Product #31430) at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection was performed using Pierce Super Signal West Pico (Product #34087).
     
 
 
PubMed References:
Immunoprecipitation
  Species / Dilution Summary
  Tag / 5mg/ml
 MA1-041 was used in immunoprecipitation to enrich for TAMRA-labeled peptides and then eluted and analyzed LC-MS/MS.
Proc Natl Acad Sci U S A.. 2005 Apr 5;102(14):4996-5001.
"High-resolution functional proteomics by active-site peptide profiling."
Author(s): Okerberg ES, Wu J, Zhang B, Samii B, Blackford K, Winn DT, Shreder KR, Burbaum JJ, Patricelli MP.
Number of Citations: 7
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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