Synuclein Antibody (syn211)

Synuclein Monoclonal Antibody for Western blot, IF, ICC, IHC (P), IP

>> See 19 other antibodies for SNCA (alpha-synuclein, alpha-synuclein, isoform NACP140, non A-beta component of AD amyloid, non-A beta component of AD amyloid, non-A4 component of amyloid)
Synonyms:
alpha-synuclein, alpha-synuclein, isoform NACP140, non A-beta component of AD amyloid, non-A beta component of AD amyloid, non-A4 component of amyloid
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Mouse / IgG1
Class: Monoclonal
Type: Antibody
Clone: syn211
Tested Species Reactivity: Human (Hu), Rat (Rt)
Immunogen: Human recombinant alpha-synuclein
Ordering Information
Pierce Synuclein Antibody (syn211)
Product #
MA5-12272
Size
500 ul
Price
$299.00
Purchase
Add Synuclein Antibody (syn211) to your cart.
Tested Applications Dilution *
Western Blot (WB) Assay Dependent
Immunofluorescence (IF) 1:20-1:200
Immunocytochemistry (ICC) 1:20-1:200
Immunohistochemistry (Paraffin) (IHC (P)) 1:200-1:400
Immunoprecipitation (IP) Assay Dependent
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 0.2mg/ml
Purification: Protein G
Storage Buffer: PBS, pH 7.4, with 0.2% BSA
Preservative: 0.09% sodium azide
Storage Conditions: 4° C, do not freeze
Product Specific Information
MA5-12272 targets Synuclein in IHC (P), ICC/IF, IP, and WB applications and shows reactivity with Human and Rat samples.

The MA5-12272 immunogen is human recombinant alpha-synuclein.
General Information
Synnuclein family of proteins is abundantly expressed in neuronal cytosol and presynaptic terminals. In vertebrates they are encoded by three different genes. Synucleins have been specifically implemented in three major diseases: Alzheimer's (AD), Parkinson's PD) and breast cancer. In AD, a peptide derived from alpha-synuclein forms an intrinsic component of plaque amyloid. In PD, an alpha synuclein accumulates in Lewy bodies. An allele of alpha synuclein has been linked to many familial cases of PD. In breast cancer increased expression of gamma synuclein correlates with the disease progression. Synucleins appear to be involved in the membrane plasticity in developing song control system of songbirds.
Product Images
  • Synuclein Antibody (MA5-12272) in IF
    MA5-12272_IF_PC12_20130724074453.jpg

    Immunofluorescence with anti-Synuclein Monoclonal Antibody [syn211] (MA5-12272) (MA5-12272_IF_PC12_20130724074453.jpg)

    Immunofluorescence with anti-Synuclein Monoclonal Antibody [syn211] (MA5-12272)

    Immunofluorescent analysis of Synuclein (green) showing staining in the cytoplasm of PC12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Synuclein monoclonal antibody (Product # MA5-12272) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
  • Synuclein Antibody (MA5-12272) in IF
    MA5-12272_IF_U251_20130724074517.jpg

    Immunofluorescence with anti-Synuclein Monoclonal Antibody [syn211] (MA5-12272) (MA5-12272_IF_U251_20130724074517.jpg)

    Immunofluorescence with anti-Synuclein Monoclonal Antibody [syn211] (MA5-12272)

    Immunofluorescent analysis of Synuclein (green) showing staining in the cytoplasm and membrane of U251 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Synuclein monoclonal antibody (Product # MA5-12272) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
  • Synuclein Antibody (MA5-12272) in IF
    MA5-12272_IF_U87-MG_20130724074538.jpg

    Immunofluorescence with anti-Synuclein Monoclonal Antibody [syn211] (MA5-12272) (MA5-12272_IF_U87-MG_20130724074538.jpg)

    Immunofluorescence with anti-Synuclein Monoclonal Antibody [syn211] (MA5-12272)

    Immunofluorescent analysis of Synuclein (green) showing staining in the cytoplasm and membrane of U87-MG cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Synuclein monoclonal antibody (Product # MA5-12272) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
  • Synuclein Antibody (MA5-12272) in IHC (P)
    MA5-12272_IHC_Human_breast_carcinoma_20130613154434.jpg

    Immunohistochemistry (Paraffin) with anti-Synuclein Monoclonal Antibody [syn211] (MA5-12272) (MA5-12272_IHC_Human_breast_carcinoma_20130613154434.jpg)

    Immunohistochemistry (Paraffin) with anti-Synuclein Monoclonal Antibody [syn211] (MA5-12272)

    Immunohistochemistry analysis of Synuclein showing positive staining in the cytoplasm and nucleus of paraffin-treated Human breast carcinoma (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Synuclein monoclonal antibody (Product # MA5-12272) diluted by 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
  • Synuclein Antibody (MA5-12272) in IHC (P)
    MA5-12272_IHC_Human_cerebellum_tissue_20130613154500.jpg

    Immunohistochemistry (Paraffin) with anti-Synuclein Monoclonal Antibody [syn211] (MA5-12272) (MA5-12272_IHC_Human_cerebellum_tissue_20130613154500.jpg)

    Immunohistochemistry (Paraffin) with anti-Synuclein Monoclonal Antibody [syn211] (MA5-12272)

    Immunohistochemistry analysis of Synuclein showing positive staining in the cytoplasm and nucleus of paraffin-treated Human cerebellum tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Synuclein monoclonal antibody (Product # MA5-12272) diluted by 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
  • Synuclein Antibody (MA5-12272) in IHC (P)
    MA5-12272_IHC_Human_tonsil_tissue_20130613154521.jpg

    Immunohistochemistry (Paraffin) with anti-Synuclein Monoclonal Antibody [syn211] (MA5-12272) (MA5-12272_IHC_Human_tonsil_tissue_20130613154521.jpg)

    Immunohistochemistry (Paraffin) with anti-Synuclein Monoclonal Antibody [syn211] (MA5-12272)

    Immunohistochemistry analysis of Synuclein showing positive staining in the cytoplasm and nucleus of paraffin-treated Human tonsil tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Synuclein monoclonal antibody (Product # MA5-12272) diluted by 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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