Synuclein Antibody (9B6)

(This product has been discontinued.)
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>> See 22 other antibodies for SNCA

Synuclein Monoclonal Antibody for Western blot, IF, IHC, ELISA

>> See 22 other antibodies for SNCA (Parkinson disease (autosomal dominant, Lewy body) 4; alpha-synuclein; non A-beta component of AD amyloid; non-A beta component of AD amyloid; non-A4 component of amyloid)
Synonyms:
Parkinson disease (autosomal dominant, Lewy body) 4; alpha-synuclein; non A-beta component of AD amyloid; non-A beta component of AD amyloid; non-A4 component of amyloid
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Mouse / IgG1
Class: Monoclonal
Type: Antibody
Clone: 9B6
Tested Species Reactivity: Human (Hu)
Immunogen: Recombinant Synuclein
Ordering Information
Pierce Synuclein Antibody (9B6)
Product #
MN1290
Size
100 ug
Price
$275.00
Purchase
(DISCONTINUED)
Tested Applications Dilution *
Western Blot (WB) Assay Dependent
Immunofluorescence (IF) 1:10-1:100
Immunohistochemistry (IHC) 1:200
ELISA (ELISA) Assay Dependent
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form information is not available for this product.
Product Specific Information
MN1290 targets Synuclein in ELISA, IHC, and WB applications and shows reactivity with Human samples.

The MN1290 immunogen is recombinant Synuclein.

MN1290 detects Synuclein which has a predicted molecular weight of approximately 14 kDa.
General Information
Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Four alternatively spliced transcripts encoding two different isoforms have been identified for this gene.
Product Images
  • Synuclein Antibody (MN1290) in IF
    Synuclein_MN1290_Immunofluorescence_Hela-Cells.jpg

    Immunofluorescence with anti-Synuclein Monoclonal Antibody [9B6] (MN1290) (Synuclein_MN1290_Immunofluorescence_Hela-Cells.jpg)

    Immunofluorescence with anti-Synuclein Monoclonal Antibody [9B6] (MN1290)

    Immunofluorescent analysis of Synuclein using Anti-Synuclein Monoclonal Antibody (9B6) (Product# MN1290) shows staining in Hela Cells. Synuclein staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Synuclein (Product# MN1290) at a dilution of 1:20 over night at 4 ◦C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35503, Goat Anti-Mouse). Images were taken at 60X magnification.
  • Synuclein Antibody (MN1290) in IHC
    MN1290_IHC_Brain_Tissue.jpg

    Immunohistochemistry with anti-Synuclein Monoclonal Antibody [9B6] (MN1290) (MN1290_IHC_Brain_Tissue.jpg)

    Immunohistochemistry with anti-Synuclein Monoclonal Antibody [9B6] (MN1290)

    Immunohistochemistry was performed on normal biopsies of deparaffinized Human brain tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Synuclein (MN1290) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  • Synuclein Antibody (MN1290) in IHC
    MN1290_IHC_Cerebellum_Tissue.jpg

    Immunohistochemistry with anti-Synuclein Monoclonal Antibody [9B6] (MN1290) (MN1290_IHC_Cerebellum_Tissue.jpg)

    Immunohistochemistry with anti-Synuclein Monoclonal Antibody [9B6] (MN1290)

    Immunohistochemistry was performed on normal biopsies of deparaffinized Human cerebellum tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Synuclein (MN1290) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific