Part of Thermo Fisher Scientific

Syntrophin Monoclonal Antibody (1351)

Syntrophin Antibody for IF, IP, Western blot

Synonyms:
SYN
Entrez Gene ID:
(Human) 6645
UniProt ID:
Q13425
Details
Host / Isotype: Mouse / IgG1
Class: Monoclonal
Type: Antibody
Clone: 1351
Species Reactivity: Human (Hu) Murine (Ms) Rat (Rt) Canine (Ca) Chicken (Ck) Amphibian (Am)
Immunogen: Whole purified syntrophin from Torpedo californica electric organ postsynaptic membrane.
Ordering Information
Pierce Syntrophin Monoclonal Antibody (1351)
Product Number Pkg. Size Price Purchase
MA1-745 100 µg $354.00
Add Syntrophin Monoclonal Antibody (1351) to your cart.


Storage: -20º C, Avoid Freeze/Thaw Cycles
Form: 100 µg of purified IgG in PBS containing 1 mg/ml BSA and 0.05% sodium azide.


Applications Dilution *
Western Blot (WB) 0.2 ug/ml
Immunoprecipitation (IP) Assay dependent
Immunofluorescence (IF) 10 ug/ml
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Product Specific Information
MA1-745 detects alpha 1, beta 1 and beta 2 subunits of syntrophin. This antibody has been shown to react with human, mouse, rat, chicken, canine and amphibian samples.

MA1-745 has been successfully used in Western blot, immunoprecipitation and immunofluorescence procedures. By Western blot, this antibody detects an ~58 kDa protein representing syntrophin from rat and mouse cell extracts.

The MA1-745 immunogen is whole purified syntrophin from Torpedo californica electric organ postsynaptic membrane. Characterization studies of this antibody have demonstrated that clone 1351 is directed against an epitope within the PDZ domain of syntrophin. MA1-745, clone 1351, is seen as the "gold standard" for syntrophin assessment.

Figure 1 illustrates Western blot detection of syntrophin in canine cell lysate using MA1-745.

Figure 2 illustrates the various protein and exon domains of the syntrophin protein.
General Information
Syntrophins are structurally related proteins located at the neuromuscular synapse and play an important role in dystrophin associated protein (DAP) complex formation. To date there have been three distinct syntrophin isoforms discovered: alpha 1, beta 1, and beta 2. These proteins each contain a PDZ domain, a syntrophin-unique domain (SU), and three pleckstrin homology domains (PH1a,1b and 2). The PDZ domain has been shown to be responsible for binding syntrophin to nNOS and the DAP complex. This association results in localization of the nicotinic acetylcholine receptor to the cytoskeleton via the (S/T)XV C terminus.
Product Images
Filter :
 
 
PubMed References:
Immunocytochemistry
  Species / Dilution Summary
  Am / Not Cited
 MA1-745 was used in immunocytochemistry to investigate the regulation of AChR cluster dispersal and formation by PTPase
J Cell Biol. 1998 Jun 29;141(7):1613-24.
"A role of tyrosine phosphatase in acetylcholine receptor cluster dispersal and formation."
Author(s): Dai Z, Peng HB
Number of Citations: 5
(See PubMed article )
Immunohistochemistry
  Species / Dilution Summary
  Ck / Not Cited
 MA1-745 was used in immunoprecipitation, western blot, immunohistochemistry to characterize the 58K protein found in AChR-rich membranes of Torpedo electrocytes and its mammalian counterpart as a skeletal muscle protein
J Cell Biol. 1987 Jun;104(6):1633-46.
"A postsynaptic Mr 58,000 (58K) protein concentrated at acetylcholine receptor-rich sites in Torpedo electroplaques and skeletal muscle."
Author(s): Froehner SC, Murnane AA, Tobler M, Peng HB, Sealock R
Number of Citations: 36
(See PubMed article )
  Ms / Not Cited
 MA1-745 was used in immunohistochemistry and western blot to study the rescue of mdx mice by wild type embryonic stem cells
PLoS One. 2009;4(3):e4759.
"Blastocyst injection of wild type embryonic stem cells induces global corrections in mdx mice."
Author(s): Stillwell E, Vitale J, Zhao Q, Beck A, Schneider J, Khadim F, Elson G, Altaf A, Yehia G, Dong JH, Liu J, Mark W, Bhaumik M, Grange R, Fraidenraich D
Number of Citations: 1
(See PubMed article )
  Rt / Not Cited
 MA1-745 was used in immunoprecipitation, western blot, immunohistochemistry to characterize the 58K protein found in AChR-rich membranes of Torpedo electrocytes and its mammalian counterpart as a skeletal muscle protein
J Cell Biol. 1987 Jun;104(6):1633-46.
"A postsynaptic Mr 58,000 (58K) protein concentrated at acetylcholine receptor-rich sites in Torpedo electroplaques and skeletal muscle."
Author(s): Froehner SC, Murnane AA, Tobler M, Peng HB, Sealock R
Number of Citations: 36
(See PubMed article )
  Rt / 1:200
 MA1-745 was used in immunohistochemistry, immunoprecipitation and western blot to study the interaction between receptor tyrosine phosphatase-like protein ICA512 and beta 2 syntrophin and nNOS in pancreatic beta-cells
Eur J Cell Biol. 2000 Sep;79(9):621-30.
"The receptor tyrosine phosphatase-like protein ICA512 binds the PDZ domains of beta2-syntrophin and nNOS in pancreatic beta-cells."
Author(s): Ort T, Maksimova E, Dirkx R, Kachinsky AM, Berghs S, Froehner SC, Solimena M
Number of Citations: 11
(See PubMed article )
Immunoprecipitation
  Species / Dilution Summary
  Ch / Not Cited
 MA1-745 was used in immunoprecipitation, western blot, immunohistochemistry to characterize the 58K protein found in AChR-rich membranes of Torpedo electrocytes and its mammalian counterpart as a skeletal muscle protein
J Cell Biol. 1987 Jun;104(6):1633-46.
"A postsynaptic Mr 58,000 (58K) protein concentrated at acetylcholine receptor-rich sites in Torpedo electroplaques and skeletal muscle."
Author(s): Froehner SC, Murnane AA, Tobler M, Peng HB, Sealock R
Number of Citations: 36
(See PubMed article )
  Hu / Not Cited
 MA1-745 was used in immunoprecipitation to study the mechanism for syntrophin regulation of alpha1D-adrenergic receptors .
J Biol Chem. 2006 May 5;281(18):12414-20.
"Syntrophins regulate alpha1D-adrenergic receptors through a PDZ domain-mediated interaction."
Author(s): Chen Z, Hague C, Hall RA, Minneman KP
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
 MA1-745 was used in immunoprecipitation and western blot to investigate the expression and distribution of syntrophins in skeletal muscles and their association with dystrophin complex
J Cell Biol. 1997 Jul 14;138(1):81-93.
"Differential association of syntrophin pairs with the dystrophin complex."
Author(s): Peters MF, Adams ME, Froehner SC
Number of Citations: 29
(See PubMed article )
  Ms / Not Cited
 MA1-745 was used in immunoprecipitation and western blot to investigate the interaction between sodium channels and dystrophin-associated proteins
J Neurosci. 1998 Jan 1;18(1):128-37.
"Interaction of muscle and brain sodium channels with multiple members of the syntrophin family of dystrophin-associated proteins."
Author(s): Gee SH, Madhavan R, Levinson SR, Caldwell JH, Sealock R, Froehner SC
Number of Citations: 49
(See PubMed article )
  Ms / Not Cited
 MA1-745 was used in immunoprecipitation to investigate the impacts of the lack of alpha-syntrophin on neuromuscular synapses
J Cell Biol. 2000 Sep 18;150(6):1385-98.
"Absence of alpha-syntrophin leads to structurally aberrant neuromuscular synapses deficient in utrophin."
Author(s): Adams ME, Kramarcy N, Krall SP, Rossi SG, Rotundo RL, Sealock R, Froehner SC
Number of Citations: 33
(See PubMed article )
  Rt / Not Cited
 MA1-745 was used in immunohistochemistry, immunoprecipitation and western blot to study the interaction between receptor tyrosine phosphatase-like protein ICA512 and beta 2 syntrophin and nNOS in pancreatic beta-cells
Eur J Cell Biol. 2000 Sep;79(9):621-30.
"The receptor tyrosine phosphatase-like protein ICA512 binds the PDZ domains of beta2-syntrophin and nNOS in pancreatic beta-cells."
Author(s): Ort T, Maksimova E, Dirkx R, Kachinsky AM, Berghs S, Froehner SC, Solimena M
Number of Citations: 11
(See PubMed article )
  Rt / Not Cited
 MA1-745 was used in immunoprecipitation and western blot to investigate the effect of insulin secretion on ICA512 and its relationship with beta 2-syntrophin and mu-calpain
EMBO J. 2001 Aug 1;20(15):4013-23.
"Dephosphorylation of beta2-syntrophin and Ca2+/mu-calpain-mediated cleavage of ICA512 upon stimulation of insulin secretion."
Author(s): Ort T, Voronov S, Guo J, Zawalich K, Froehner SC, Zawalich W, Solimena M
Number of Citations: 1
(See PubMed article )
Western Blot
  Species / Dilution Summary
  Ch / Not Cited
 MA1-745 was used in immunoprecipitation, western blot, immunohistochemistry to characterize the 58K protein found in AChR-rich membranes of Torpedo electrocytes and its mammalian counterpart as a skeletal muscle protein
J Cell Biol. 1987 Jun;104(6):1633-46.
"A postsynaptic Mr 58,000 (58K) protein concentrated at acetylcholine receptor-rich sites in Torpedo electroplaques and skeletal muscle."
Author(s): Froehner SC, Murnane AA, Tobler M, Peng HB, Sealock R
Number of Citations: 36
(See PubMed article )
  Ms / 30 nM
 MA1-745 was used in immunoprecipitation and western blot to investigate the expression and distribution of syntrophins in skeletal muscles and their association with dystrophin complex
J Cell Biol. 1997 Jul 14;138(1):81-93.
"Differential association of syntrophin pairs with the dystrophin complex."
Author(s): Peters MF, Adams ME, Froehner SC
Number of Citations: 29
(See PubMed article )
  Ms / Not Cited
 MA1-745 was used in immunoprecipitation and western blot to investigate the interaction between sodium channels and dystrophin-associated proteins
J Neurosci. 1998 Jan 1;18(1):128-37.
"Interaction of muscle and brain sodium channels with multiple members of the syntrophin family of dystrophin-associated proteins."
Author(s): Gee SH, Madhavan R, Levinson SR, Caldwell JH, Sealock R, Froehner SC
Number of Citations: 49
(See PubMed article )
  Ms / Not Cited
 MA1-745 was used in western blot to investigate the role of syntrophins and dystrophin on the proper expression and function of Na (v) 1.5
Circ Res. 2006 Aug 18;99(4):407-14.
"Cardiac sodium channel Nav1.5 is regulated by a multiprotein complex composed of syntrophins and dystrophin."
Author(s): Gavillet B, Rougier JS, Domenighetti AA, Behar R, Boixel C, Ruchat P, Lehr HA, Pedrazzini T, Abriel H
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
 MA1-745 was used in immunohistochemistry and western blot to study the rescue of mdx mice by wild type embryonic stem cells
PLoS One. 2009;4(3):e4759.
"Blastocyst injection of wild type embryonic stem cells induces global corrections in mdx mice."
Author(s): Stillwell E, Vitale J, Zhao Q, Beck A, Schneider J, Khadim F, Elson G, Altaf A, Yehia G, Dong JH, Liu J, Mark W, Bhaumik M, Grange R, Fraidenraich D
Number of Citations: 1
(See PubMed article )
  Rt / 1:5,000
 MA1-745 was used in immunohistochemistry, immunoprecipitation and western blot to study the interaction between receptor tyrosine phosphatase-like protein ICA512 and beta 2 syntrophin and nNOS in pancreatic beta-cells
Eur J Cell Biol. 2000 Sep;79(9):621-30.
"The receptor tyrosine phosphatase-like protein ICA512 binds the PDZ domains of beta2-syntrophin and nNOS in pancreatic beta-cells."
Author(s): Ort T, Maksimova E, Dirkx R, Kachinsky AM, Berghs S, Froehner SC, Solimena M
Number of Citations: 11
(See PubMed article )
  Rt / 1:5,000
 MA1-745 was used in immunoprecipitation and western blot to investigate the effect of insulin secretion on ICA512 and its relationship with beta 2-syntrophin and mu-calpain
EMBO J. 2001 Aug 1;20(15):4013-23.
"Dephosphorylation of beta2-syntrophin and Ca2+/mu-calpain-mediated cleavage of ICA512 upon stimulation of insulin secretion."
Author(s): Ort T, Voronov S, Guo J, Zawalich K, Froehner SC, Zawalich W, Solimena M
Number of Citations: 1
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)