Surfactant Protein B (Precursor) Antibody, Biotin conjugate (SPB01 (1-2-A5-9))

Surfactant Protein B (Precursor) Monoclonal Antibody for IF, IHC (P)

>> See 3 other antibodies for SFTPB (18 kDa pulmonary-surfactant protein, 6 kDa protein,pulmonary surfactant-associated protein B, pulmonary surfactant-associated proteolipid SPL(Phe))
Synonyms:
18 kDa pulmonary-surfactant protein, 6 kDa protein,pulmonary surfactant-associated protein B, pulmonary surfactant-associated proteolipid SPL(Phe)
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Mouse / IgG2a, kappa
Class: Monoclonal
Type: Antibody
Clone: SPB01 (1-2-A5-9)
Label: Biotin
Tested Species Reactivity: Human (Hu), Rat (Rt)
Published Species Reactivity: Human (Hu)
Immunogen: Recombinant pro-surfactant protein B
Ordering Information
Pierce Surfactant Protein B (Precursor) Antibody, Biotin conjugate (SPB01 (1-2-A5-9))
Product #
MA5-13972
Size
500 ul
Price
$399.00
Purchase
Add Surfactant Protein B (Precursor) Antibody, Biotin conjugate (SPB01 (1-2-A5-9)) to your cart.
Tested Applications Dilution *
Immunofluorescence (IF) Assay Dependent
Immunohistochemistry (Paraffin) (IHC (P)) 2-4 µg/ml
Published Applications Dilution
Immunohistochemistry (IHC) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 0.2mg/ml
Purification: Protein A
Storage Buffer: PBS, pH 7.4, with 0.2% BSA
Preservative: 0.09% sodium azide
Storage Conditions: 4° C
Product Specific Information
MA5-13972 targets Surfactant Protein B (Precursor) in IF and IHC (P) applications and shows reactivity with Human and Rat samples.

The MA5-13972 immunogen is recombinant pro-surfactant protein B.
General Information
Pulmonary surfactant is a complex mixture of phospholipids and proteins that is secreted from type II cells in alveoli and reduces the surface tension at the alveolar air-liquid interface, providing alveolar stability necessary for normal ventillation. Four distinct proteins isolated from pulmonary surfactant are termed surfactant proteins A, B, C, and D. SP-A (28-36kDa) and SP-D (43kDa) are collagenous carbohydrate-binding proteins, whereas SP-B (8-9kDa) and SP-C (4kDa) are non-collagenous hydrophobic proteins. SP-B is expressed in pulmonary adenocarcinomas with acinar, papillary, bronchioloalveolar, and solid growth patterns. Squamous cell and large cell carcinomas of the lung and nonpulmonary adenocarcinomas do not express SP-B. ProSP-B is glycosylated in the Golgi apparatus and undergoes carboxy- and amino-terminal proteolysis by a cathepsin D-like protease.
Product Images
  • Surfactant Protein B (Precursor) Antibody (MA5-13972) in IHC
    MA5-13972_Immunohistochemistry.jpg

    Immunohistochemistry with anti-Surfactant Protein B (Precursor) Monoclonal Antibody [SPB01 (1-2-A5-9)], Biotin conjugate (MA5-13972) (MA5-13972_Immunohistochemistry.jpg)

    Immunohistochemistry with anti-Surfactant Protein B (Precursor) Monoclonal Antibody [SPB01 (1-2-A5-9)], Biotin conjugate (MA5-13972)

    Formalin-fixed, paraffin-embedded human lung stained with Prosurfactant Protein B antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of alveolar type-II epithelial cells.
  • Surfactant Protein B (Precursor) Antibody (MA5-13972) in IHC
    MA5-13972_Immunohistochemistry2.jpg

    Immunohistochemistry with anti-Surfactant Protein B (Precursor) Monoclonal Antibody [SPB01 (1-2-A5-9)], Biotin conjugate (MA5-13972) (MA5-13972_Immunohistochemistry2.jpg)

    Immunohistochemistry with anti-Surfactant Protein B (Precursor) Monoclonal Antibody [SPB01 (1-2-A5-9)], Biotin conjugate (MA5-13972)

    Formalin-fixed, paraffin-embedded rat lung stained with Prosurfactant Protein B antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of alveolar type-II cells.
  • Surfactant Protein B (Precursor) Antibody (MA5-13972) in IHC
    MA5-13972_Immunohistochemistry_2.jpg

    Immunohistochemistry with anti-Surfactant Protein B (Precursor) Monoclonal Antibody [SPB01 (1-2-A5-9)], Biotin conjugate (MA5-13972) (MA5-13972_Immunohistochemistry_2.jpg)

    Immunohistochemistry with anti-Surfactant Protein B (Precursor) Monoclonal Antibody [SPB01 (1-2-A5-9)], Biotin conjugate (MA5-13972)

Publications:
Immunohistochemistry
  Species / Dilution Summary
  Hu / 1:50
MA5-13972 was used in immunohistochemistry to report a clinical case of basaloid adenocarcinoma

Virchows Arch. 2007 Sep;451(3):729-36.
"Basaloid adenocarcinoma. A new variant of pulmonary adenocarcinoma."
Author(s): Marci V, Volante M, Cappia S, Righi L, Novello C, Scagliotti GV, Brambilla E, Papotti M
Number of Citations: 0
(See PubMed article )
  Hu / 1:25
MA5-13972 was used in immunohistochemistry to examine the diagnostic significance of thyroid transcription factor-1 in ovarian and uterine epithelial neoplasia

Int J Gynecol Pathol. 2009 Jan;28(1):10-8.
"TTF-1 expression in ovarian and uterine epithelial neoplasia and its potential significance, an immunohistochemical assessment with multiple monoclonal antibodies and different secondary detection systems."
Author(s): Zhang PJ, Gao HG, Pasha TL, Litzky L, Livolsi VA
Number of Citations: 2
(See PubMed article )
  Hu / Not Cited
MA5-13972 was used in immunohistochemistry to investigate the role of TTF1 in lung surfactant homeostasis and respiratory system integrity

Respir Res. 2011 Aug 25;12():115.
"Altered surfactant homeostasis and recurrent respiratory failure secondary to TTF-1 nuclear targeting defect."
Author(s): Peca D, Petrini S, Tzialla C, Boldrini R, Morini F, Stronati M, Carnielli VP, Cogo PE, Danhaive O
Number of Citations: 1
(See PubMed article )
  Hu / 1:100
MA5-13972 was used in immunohistochemistry to study the effect of narrow-band UVB phototherapy on the immunohistochemical expresson of surfactant proteins A and B in psoriasis patients

Am J Clin Dermatol. 2012 Oct 1;13(5):341-8.
"Immunohistochemical staining of surfactant proteins A and B in skin of psoriatic patients before and after narrow-band UVB phototherapy."
Author(s): Aiad HA, El-Farargy SM, Soliman MM, El-Wahed Gaber MA, El-Aziz Othman SA
Number of Citations: 0
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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