Sodium / Potassium ATPase alpha-3 Antibody (XVIF9-G10)

Sodium / Potassium ATPase alpha-3 Monoclonal Antibody for Western blot, IF, IHC (F), FACS

Synonyms:
EC 3.6.3.9, Sodium pump 3, Na+/K+ ATPase 3, Alpha(III)
Details
Host / Isotype: Mouse / IgG1
Class: Monoclonal
Type: Antibody
Clone: XVIF9-G10
Tested Species Reactivity: Human (Hu), Mouse (Ms), Rat (Rt), Bovine (Bv), Canine (Ca), Guinea Pig (GP), Non-human primate (Nhp), Ovine (Ov), Rabbit (Rb)
Published Species Reactivity: Amphibian (Am), Bovine (Bv), Canine (Ca), Guinea Pig (GP), Human (Hu), Mouse (Ms), Non-human primate (Nhp), Porcine (Po), Rabbit (Rb), Rat (Rt), Shark (Sh)
Immunogen: Canine cardiac microsomes.
Ordering Information
Pierce Sodium / Potassium ATPase alpha-3 Antibody (XVIF9-G10)
Product #
MA3-915
Size
100 µg
Price
$360.00
Purchase
Add Sodium / Potassium ATPase alpha-3 Antibody (XVIF9-G10) to your cart.
Tested Applications Dilution *
Western Blot (WB) 1 µg/ml
Immunofluorescence (IF) 5 ug/ml
Immunohistochemistry (Frozen) (IHC (F)) 3 µg/ml
Flow Cytometry (FACS) 1µg per 10^6 cells
Published Applications Dilution
Western Blot (WB) See publications below
Immunocytochemistry (ICC) See publications below
Immunohistochemistry (IHC) See publications below
Immunoprecipitation (IP) See publications below
Blocking Assay (BLOCK) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 1mg/ml
Purification: Protein A
Storage Buffer: 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Preservative: 0.05% sodium azide
Storage Conditions: -20° C, Avoid Freeze/Thaw Cycles
Product Specific Information
MA3-915 detects sodium/potassium ATPase from human, monkey, bovine, sheep, canine, rabbit, guinea pig, mouse and rat tissue. This antibody is specific for the alpha-3 subunit.

MA3-915 has been successfully used in Western blot and immunohistochemical procedures. By Western blot, this antibody detects an ~110 kDa protein representing the alpha-3 subunit of the sodium/potassium ATPase from canine skeletal muscle extract. Immunohistochemical staining of sodium/potassium ATPase in rat retina with MA3-915 yields a pattern consistent with plasma membrane localization.

The MA3-915 antigen is canine cardiac microsomes.
General Information
The sodium/potassium ATPase is an integral membrane enzyme found in all cells of higher organisms and is responsible for the ATP-dependent transport of sodium and potassium across the cell membrane. This membrane-bound enzyme is related to a number of other ATPases including sarcoplasmic and endoplasmic reticulum calcium ATPase (SERCA) and plasma membrane calcium ATPase (PMCA). The sodium/potassium ATPase consists of a large, multipass, transmembrane catalytic subunit, termed the alpha subunit, and an associated smaller glycoprotein, termed the beta subunit. Studies indicate that there are three isoforms of the alpha subunit (alpha 1, alpha 2, alpha 3) and two isoforms of the beta subunit (beta 1 and beta 2) encoded by two multigene families.

The different isoforms of the sodium/potassium ATPase exhibit tissue-specific and developmental patterns of expression. The alpha 1 and beta mRNAs are present in all cell types examined, whereas the alpha 2 and alpha 3 mRNAs exhibit a more restricted pattern of cell-specific expression. The alpha-3 subunit has been found in neuronal and to skeletal and cardiac muscle, lung and stomach tissues.
Product Images
  • Sodium / Potassium ATPase alpha-3 Antibody (MA3-915) in IF
    MA3-915_IF_C6.jpg

    Immunofluorescence with anti-Sodium  /  Potassium ATPase alpha-3 Monoclonal Antibody [XVIF9-G10] (MA3-915) (MA3-915_IF_C6.jpg)

    Immunofluorescence with anti-Sodium / Potassium ATPase alpha-3 Monoclonal Antibody [XVIF9-G10] (MA3-915)

    Immunofluorescent analysis of Sodium/Potassium ATPase alpha-3 using Sodium/Potassium ATPase alpha-3 Monoclonal antibody (XVIF9-G10) (Product# MA3-915) shows staining in C6 glioma cells. Sodium/Potassium ATPase alpha-3 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Sodium/Potassium ATPase alpha-3 (Product# MA3-915) at a dilution of 1:20 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.
  • Sodium / Potassium ATPase alpha-3 Antibody (MA3-915) in IF
    MA3-915_IF_Hela.jpg

    Immunofluorescence with anti-Sodium  /  Potassium ATPase alpha-3 Monoclonal Antibody [XVIF9-G10] (MA3-915) (MA3-915_IF_Hela.jpg)

    Immunofluorescence with anti-Sodium / Potassium ATPase alpha-3 Monoclonal Antibody [XVIF9-G10] (MA3-915)

    Immunofluorescent analysis of Sodium/Potassium ATPase alpha-3 using Sodium/Potassium ATPase alpha-3 Monoclonal antibody (XVIF9-G10) (Product# MA3-915) shows staining in HeLa cells. Sodium/Potassium ATPase alpha-3 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Sodium/Potassium ATPase alpha-3 (Product# MA3-915) at a dilution of 1:20 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.
  • Sodium / Potassium ATPase alpha-3 Antibody (MA3-915) in IF
    MA3-915_IF_U251.jpg

    Immunofluorescence with anti-Sodium  /  Potassium ATPase alpha-3 Monoclonal Antibody [XVIF9-G10] (MA3-915) (MA3-915_IF_U251.jpg)

    Immunofluorescence with anti-Sodium / Potassium ATPase alpha-3 Monoclonal Antibody [XVIF9-G10] (MA3-915)

    Immunofluorescent analysis of Sodium/Potassium ATPase alpha-3 using Sodium/Potassium ATPase alpha-3 Monoclonal antibody (XVIF9-G10) (Product# MA3-915) shows staining in U251 glioma cells. Sodium/Potassium ATPase alpha-3 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Sodium/Potassium ATPase alpha-3 (Product# MA3-915) at a dilution of 1:20 over night at 4 ?C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.
  • Sodium / Potassium ATPase alpha-3 Antibody (MA3-915) in IHC
    MA3-915_Immunohistochemistry_Tonsil-tissue.jpg

    Immunohistochemistry with anti-Sodium  /  Potassium ATPase alpha-3 Monoclonal Antibody [XVIF9-G10] (MA3-915) (MA3-915_Immunohistochemistry_Tonsil-tissue.jpg)

    Immunohistochemistry with anti-Sodium / Potassium ATPase alpha-3 Monoclonal Antibody [XVIF9-G10] (MA3-915)

    Immunohistochemistry was performed on normal deparaffinized Human tonsil tissue tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Sodium/Potassium ATPase alpha-3 (MA3-915) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  • Sodium / Potassium ATPase alpha-3 Antibody (MA3-915) in IHC
    MA3-915_Immunohistochemistry_Colon-tissue.jpg

    Immunohistochemistry with anti-Sodium  /  Potassium ATPase alpha-3 Monoclonal Antibody [XVIF9-G10] (MA3-915) (MA3-915_Immunohistochemistry_Colon-tissue.jpg)

    Immunohistochemistry with anti-Sodium / Potassium ATPase alpha-3 Monoclonal Antibody [XVIF9-G10] (MA3-915)

    Immunohistochemistry was performed on normal deparaffinized Human colon tissue tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Sodium/Potassium ATPase alpha-3 (MA3-915) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
  • Sodium / Potassium ATPase alpha-3 Antibody (MA3-915) in IHC
    MA3-915_Immunohistochemistry_Prostase-carcinoma.jpg

    Immunohistochemistry with anti-Sodium  /  Potassium ATPase alpha-3 Monoclonal Antibody [XVIF9-G10] (MA3-915) (MA3-915_Immunohistochemistry_Prostase-carcinoma.jpg)

    Immunohistochemistry with anti-Sodium / Potassium ATPase alpha-3 Monoclonal Antibody [XVIF9-G10] (MA3-915)

    Immunohistochemistry was performed on cancer biopsies of deparaffinized Human prostate carcinoma tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:50 with a mouse monoclonal antibody recognizing Sodium/Potassium ATPase alpha-3 (MA3-915) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Publications:
Western Blot
  Species / Dilution Summary
  Am / Not Cited
MA3-915 was used in western blot to investigate the role of the aldosterone during the activation of sodium pumps

J Physiol. 1999 May 1;516 ( Pt 3)():647-55.
"Regulation of Na+ pump function by aldosterone is alpha-subunit isoform specific."
Author(s): Pfeiffer R, Beron J, Verrey F
Number of Citations: 1
(See PubMed article )
  Bv / Not Cited
MA3-915 was used in western blot and immunohistochemistry to study the localization of the sodium pump alpha-isoform in whole lens and within the major structural regions of lens

Hypertension. 1999 Nov;34(5):1168-74.
"Sodium pump inhibition and regional expression of sodium pump alpha-isoforms in lens."
Author(s): Tao QF, Hollenberg NK, Graves SW
Number of Citations: 0
(See PubMed article )
  Bv / Not Cited
MA3-915 was used in western blot and immunohistochemistry to investigate the localization of retinoschisin (RS1) on the surface of retinal photoreceptor and bipolar cells

J Biol Chem. 2007 Nov 9;282(45):32792-801.
"Retinoschisin (RS1), the protein encoded by the X-linked retinoschisis gene, is anchored to the surface of retinal photoreceptor and bipolar cells through its interactions with a Na/K ATPase-SARM1 complex."
Author(s): Molday LL, Wu WW, Molday RS
Number of Citations: 1
(See PubMed article )
  Ca / Not Cited
Ma3-915 was used in western blot and immunocytochemistry to profile the sodium pump isoforms in human erythroid progenitor cells and mature erythrocytes.

Proc Natl Acad Sci U S A. 2002 Oct 29;99(22):14572-7.
"Na pump isoforms in human erythroid progenitor cells and mature erythrocytes."
Author(s): Hoffman JF, Wickrema A, Potapova O, Milanick M, Yingst DR
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
MA3-915 was used in western blot and immunohistochemistry to study the localization of the sodium pump alpha-isoform in whole lens and within the major structural regions of lens

Hypertension. 1999 Nov;34(5):1168-74.
"Sodium pump inhibition and regional expression of sodium pump alpha-isoforms in lens."
Author(s): Tao QF, Hollenberg NK, Graves SW
Number of Citations: 0
(See PubMed article )
  Hu / 1:500
MA3-915 was used in western blot to investigate the effects of PKC-induced-specific NKA isoform phosphorylation on NKA inhibition and vasoconstriction

Hypertension. 2002 Feb;39(2):298-302.
"Phorbol diacetate potentiates na(+)-k(+) ATPase inhibition by a putative endogenous ligand, marinobufagenin."
Author(s): Fedorova OV, Dorofeeva NA, Lopatin DA, Lakatta EG, Bagrov AY
Number of Citations: 2
(See PubMed article )
  Hu / Not Cited
MA3-915 was used in western blot to investigate the effect of cycloheximide on sodium/potassium ATPase activity.

Invest Ophthalmol Vis Sci. 2002 Aug;43(8):2714-20.
"The influence of cycloheximide on Na,K-ATPase activity in cultured human lens epithelial cells."
Author(s): Cui G, Dean WL, Delamere NA
Number of Citations: 0
(See PubMed article )
  Hu / 1:1,000
Ma3-915 was used in western blot and immunocytochemistry to profile the sodium pump isoforms in human erythroid progenitor cells and mature erythrocytes.

Proc Natl Acad Sci U S A. 2002 Oct 29;99(22):14572-7.
"Na pump isoforms in human erythroid progenitor cells and mature erythrocytes."
Author(s): Hoffman JF, Wickrema A, Potapova O, Milanick M, Yingst DR
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
MA3-915 was used in western blot to investigate the effect of acute exercise effects on sodium/potassium-ATPase isoform expression in human skeletal muscle.

J Physiol. 2004 Apr 15;556(Pt 2):507-19.
"Intense exercise up-regulates Na+,K+-ATPase isoform mRNA, but not protein expression in human skeletal muscle."
Author(s): Murphy KT, Snow RJ, Petersen AC, Murphy RM, Mollica J, Lee JS, Garnham AP, Aughey RJ, Leppik JA, Medved I, Cameron-Smith D, McKenna MJ
Number of Citations: 1
(See PubMed article )
  Hu / 0
MA3-915 was used in western blot to study the association of mutations in the Na+/K+-ATPase alpha 3 subunit with rapid-onset dystonia Parkinsonism

Neuron. 2004 Jul 22;43(2):169-75.
"Mutations in the Na+/K+ -ATPase alpha3 gene ATP1A3 are associated with rapid-onset dystonia parkinsonism."
Author(s): de Carvalho Aguiar P, Sweadner KJ, Penniston JT, Zaremba J, Liu L, Caton M, Linazasoro G, Borg M, Tijssen MA, Bressman SB, Dobyns WB, Brashear A, Ozelius LJ
Number of Citations: 30
(See PubMed article )
  Hu / Not Cited
MA3-915 was used in western blot to study the interplay between calcium homeostasis and sodium pump activity in a human megakaryocytic cell line

Am J Physiol Cell Physiol. 2005 Oct;289(4):C891-7.
"Calcium adaptation to sodium pump inhibition in a human megakaryocytic cell line."
Author(s): Kimura M, Cao X, Aviv A
Number of Citations: 1
(See PubMed article )
  Hu / 1:10,000
MA3-915 was used in western blot to study the role of prolonged submaximal exercise on the expression level of sodium/potassium-ATPase mRNA and protein in human skeletal muscle

Am J Physiol Regul Integr Comp Physiol. 2006 Feb;290(2):R414-24.
"Prolonged submaximal exercise induces isoform-specific Na+-K+-ATPase mRNA and protein responses in human skeletal muscle."
Author(s): Murphy KT, Petersen AC, Goodman C, Gong X, Leppik JA, Garnham AP, Cameron-Smith D, Snow RJ, McKenna MJ
Number of Citations: 1
(See PubMed article )
  Hu / 1:500
MA3-915 was used in western blot to investigate the expression, localization and function of Na,K-ATPase alpha 4 isoform in human spermatozoa

Mol Hum Reprod. 2006 Sep;12(9):565-76.
"The Na,K-ATPase alpha4 isoform from humans has distinct enzymatic properties and is important for sperm motility."
Author(s): Sanchez G, Nguyen AN, Timmerberg B, Tash JS, Blanco G
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
MA3-915 was used in western blot to investigate the effect of ouabain on the cell proliferation and MEK-ERK pathway of human normal and polycystic kidney cells

J Am Soc Nephrol. 2007 Jan;18(1):46-57.
"Ouabain binds with high affinity to the Na,K-ATPase in human polycystic kidney cells and induces extracellular signal-regulated kinase activation and cell proliferation."
Author(s): Nguyen AN, Wallace DP, Blanco G
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
MA3-915 was used in western blot to demonstrate that the upregulation of sodium/potassium-ATPase alpha mRNA play a role in responding to intense interval exercise and training in athletes

J Appl Physiol. 2007 Jul;103(1):39-47.
"Muscle Na+-K+-ATPase activity and isoform adaptations to intense interval exercise and training in well-trained athletes."
Author(s): Aughey RJ, Murphy KT, Clark SA, Garnham AP, Snow RJ, Cameron-Smith D, Hawley JA, McKenna MJ
Number of Citations: 1
(See PubMed article )
  Hu / 0
MA3-915 was used in western blot to study the role of sodium/potassium ATPase alpha2beta1 in calcium homeostasis in caveolae vesicles

Life Sci. 2009 Jan 30;84(5-6):139-48.
"Ca2+ influx mechanisms in caveolae vesicles of pulmonary smooth muscle plasma membrane under inhibition of alpha2beta1 isozyme of Na+/K+-ATPase by ouabain."
Author(s): Ghosh B, Kar P, Mandal A, Dey K, Chakraborti T, Chakraborti S
Number of Citations: 1
(See PubMed article )
  Hu / 0
MA3-915 was used in western blot to investigate the relationship between translationally controlled tumor protein expression in lens epithelial cells and cataract development

Transgenic Res. 2009 Dec;18(6):953-60.
"Over-expression of translationally controlled tumor protein in lens epithelial cells seems to be associated with cataract development."
Author(s): Kim MJ, Lyu J, Sohn KB, Kim M, Cho MC, Joo CK, Lee K
Number of Citations: 1
(See PubMed article )
  Hu / 1:1000
MA3-915 was used in western blot to investigate the effect of alpha 3NaK overexpression on sodium/potassium ATPase activity of hepatocellular carcinoma cells

Eur J Pharmacol. 2010 Jul 25;638(1-3):42-6.
"Increase in ouabain-sensitive K+-ATPase activity in hepatocellular carcinoma by overexpression of Na+, K+-ATPase alpha 3-isoform."
Author(s): Shibuya K, Fukuoka J, Fujii T, Shimoda E, Shimizu T, Sakai H, Tsukada K
Number of Citations: 1
(See PubMed article )
  Hu / 0
MA3-915 was used in western blot to investigate the effect of aldosterone on sodium/potassium-ATPase activity

Clin Endocrinol (Oxf). 2011 Feb;74(2):152-9.
"Aldosterone increases Na+ -K+ -ATPase activity in skeletal muscle of patients with Conn's syndrome."
Author(s): Phakdeekitcharoen B, Kittikanokrat W, Kijkunasathian C, Chatsudthipong V
Number of Citations: 1
(See PubMed article )
  Hu / 0
MA3-915 was used in western blot to investigate the relationship between abnormal skeletal muscle Na+,K+-pumps and impaired exercise performance in hemodialysis patients and renal transplant recipients

Nephrol Dial Transplant. 2012 May;27(5):2036-43.
"Impaired exercise performance and muscle Na(+),K(+)-pump activity in renal transplantation and haemodialysis patients."
Author(s): Petersen AC, Leikis MJ, McMahon LP, Kent AB, Murphy KT, Gong X, McKenna MJ
Number of Citations: 1
(See PubMed article )
  Hu / Not Cited
MA3-915 was used in western blot to study the reduced abundance of the Na(+),K(+) pump alpha2 subunit in aged human skeletal muscle

J Appl Physiol (1985). 2012 Nov;113(10):1505-11.
"Unchanged [3H]ouabain binding site content but reduced Na+-K+ pump ?2-protein abundance in skeletal muscle in older adults."
Author(s): McKenna MJ, Perry BD, Serpiello FR, Caldow MK, Levinger P, Cameron-Smith D, Levinger I
Number of Citations: 1
(See PubMed article )
  Ms / 0
MA3-915 was used in western blot to study the role of the sodium/potassium ATPase alpha-2 in calcium signaling during cardiac contraction

Mol Cell. 1999 May;3(5):555-63.
"Identification of a specific role for the Na,K-ATPase alpha 2 isoform as a regulator of calcium in the heart."
Author(s): James PF, Grupp IL, Grupp G, Woo AL, Askew GR, Croyle ML, Walsh RA, Lingrel JB
Number of Citations: 37
(See PubMed article )
  Ms / 1:1,000
MA3-915 was used in western blot to investigate sodium/potassium-ATPase alpha 1- and alpha 2-isoforms' specific functional properties

Am J Physiol Regul Integr Comp Physiol. 2001 Sep;281(3):R917-25.
"The alpha(1)- and alpha(2)-isoforms of Na-K-ATPase play different roles in skeletal muscle contractility."
Author(s): He S, Shelly DA, Moseley AE, James PF, James JH, Paul RJ, Lingrel JB
Number of Citations: 6
(See PubMed article )
  Ms / Not Cited
MA3-915 was used in western blot to investigate the differences of the sodium/potassium ATPase subunits alpha1, alpha2, and alpha3 in both mutant and wild-type mice.

Invest Ophthalmol Vis Sci. 2002 May;43(5):1517-9.
"Altered Na,K-ATPase pattern in gamma-crystallin mutant mice."
Author(s): Moseley A, Graw J, Delamere NA
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
MA3-915 was used in western blot to investigate the role of Na,K-ATPase alpha 2 isoform in the modulation of neuronal activity in the neonate

J Biol Chem. 2003 Feb 14;278(7):5317-24.
"The Na,K-ATPase alpha 2 isoform is expressed in neurons, and its absence disrupts neuronal activity in newborn mice."
Author(s): Moseley AE, Lieske SP, Wetzel RK, James PF, He S, Shelly DA, Paul RJ, Boivin GP, Witte DP, Ramirez JM, Sweadner KJ, Lingrel JB
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
MA3-915 was used in western blot to study the effect of sodium/potassium ATPase on ouabain-induced cardiac contractility in mice.

J Biol Chem. 2003 Dec 26;278(52):53026-34.
"The alpha2 isoform of Na,K-ATPase mediates ouabain-induced cardiac inotropy in mice."
Author(s): Dostanic I, Lorenz JN, Schultz Jel J, Grupp IL, Neumann JC, Wani MA, Lingrel JB
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
MA3-915 was used in western blot and immunohistochemistry to study the localization and transport of choline transporters in cholinergic terminals.

J Neurosci. 2003 Oct 29;23(30):9697-709.
"Vesicular localization and activity-dependent trafficking of presynaptic choline transporters."
Author(s): Ferguson SM, Savchenko V, Apparsundaram S, Zwick M, Wright J, Heilman CJ, Yi H, Levey AI, Blakely RD
Number of Citations: 12
(See PubMed article )
  Ms / Not Cited
MA3-915 was used in western blot to investigate the importance of various sodium-pump isoforms in vascular function

Am J Physiol Cell Physiol. 2004 Apr;286(4):C813-20.
"Na(+) pump alpha 2-isoform specifically couples to contractility in vascular smooth muscle: evidence from gene-targeted neonatal mice."
Author(s): Shelly DA, He S, Moseley A, Weber C, Stegemeyer M, Lynch RM, Lingrel J, Paul RJ
Number of Citations: 1
(See PubMed article )
  Ms / 1:1,000
MA3-915 was used in western blot to study the role of the sodium/potassium-ATPase alpha2-subunit isoform during muscle contraction

Am J Physiol Cell Physiol. 2004 Nov;287(5):C1300-10.
"The Na(+)-K(+)-ATPase alpha2-subunit isoform modulates contractility in the perinatal mouse diaphragm."
Author(s): Radzyukevich TL, Moseley AE, Shelly DA, Redden GA, Behbehani MM, Lingrel JB, Paul RJ, Heiny JA
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
MA3-915 was used in western blot to study the role of the sodium potassium ATPase alpha 1 isoform in heart.

J Biol Chem. 2004 Dec 24;279(52):54053-61.
"The alpha 1 isoform of Na,K-ATPase regulates cardiac contractility and functionally interacts and co-localizes with the Na/Ca exchanger in heart."
Author(s): Dostanic I, Schultz Jel J, Lorenz JN, Lingrel JB
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
MA3-915 was used in western blot to study the role of the sodium/potassium ATPase alpha isoform in spatial learning, motor activity, and anxiety in mice.

J Neurosci. 2007 Jan 17;27(3):616-26.
"Deficiency in Na,K-ATPase alpha isoform genes alters spatial learning, motor activity, and anxiety in mice."
Author(s): Moseley AE, Williams MT, Schaefer TL, Bohanan CS, Neumann JC, Behbehani MM, Vorhees CV, Lingrel JB
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
MA3-915 was used in western blot and immunohistochemistry to investigate the localization of retinoschisin (RS1) on the surface of retinal photoreceptor and bipolar cells

J Biol Chem. 2007 Nov 9;282(45):32792-801.
"Retinoschisin (RS1), the protein encoded by the X-linked retinoschisis gene, is anchored to the surface of retinal photoreceptor and bipolar cells through its interactions with a Na/K ATPase-SARM1 complex."
Author(s): Molday LL, Wu WW, Molday RS
Number of Citations: 1
(See PubMed article )
  Ms / 1:3,000
MA3-915 was used in western blot to study the effect of ouabain-like substance on the expression of sodium-potassium-ATPase isoforms.

Am J Physiol Regul Integr Comp Physiol. 2009 May;296(5):R1427-38.
"Enhanced pressor response to increased CSF sodium concentration and to central ANG I in heterozygous alpha2 Na+ -K+ -ATPase knockout mice."
Author(s): Hou X, Theriault SF, Dostanic-Larson I, Moseley AE, Lingrel JB, Wu H, Dean S, Van Huysse JW
Number of Citations: 1
(See PubMed article )
  Ms / 0
MA3-915 was used in immunocytochemistry and western blot to investigate the accumulation of huntingtin and GSK 3-beta mutants in murine Huntington model

J Neurosci Res. 2010 Jan;88(1):179-90.
"Mutant huntingtin and glycogen synthase kinase 3-beta accumulate in neuronal lipid rafts of a presymptomatic knock-in mouse model of Huntington's disease."
Author(s): Valencia A, Reeves PB, Sapp E, Li X, Alexander J, Kegel KB, Chase K, Aronin N, DiFiglia M
Number of Citations: 1
(See PubMed article )
  Ms / Not Cited
MA3-915 was used in western blot to investigate the role of caveolin in the function of sodium potassium ATPase in mice

J Mol Cell Cardiol. 2010 Sep;49(3):525-31.
"Alterations of Na+/K+-ATPase function in caveolin-1 knockout cardiac fibroblasts."
Author(s): Quintas LE, Pierre SV, Liu L, Bai Y, Liu X, Xie ZJ
Number of Citations: 1
(See PubMed article )
  Ms / 1:1000
MA3-915 was used in western blot to investigate the role alpha2 sodium/potassium-ATPase domains on blood pressure

Am J Hypertens. 2010 Dec;23(12):1279-85.
"The ouabain-binding site of the ?2 isoform of Na,K-ATPase plays a role in blood pressure regulation during pregnancy."
Author(s): Oshiro N, Dostanic-Larson I, Neumann JC, Lingrel JB
Number of Citations: 1
(See PubMed article )
  Ms / 0
MA3-915 was used in western blot to investigate the cortical spreading depression in familial hemiplegic migraine type 2 mouse model

PLoS Genet. 2011 Jun;7(6):e1002129.
"Increased susceptibility to cortical spreading depression in the mouse model of familial hemiplegic migraine type 2."
Author(s): Leo L, Gherardini L, Barone V, De Fusco M, Pietrobon D, Pizzorusso T, Casari G
Number of Citations: 1
(See PubMed article )
  Ms / 0
MA3-915 was used in western blot to study the effect of cardiac glycoside oleandrin from Nerium oleander on brain slice-based strokes

J Neurochem. 2011 Nov;119(4):805-14.
"In vitro and in vivo neuroprotective activity of the cardiac glycoside oleandrin from Nerium oleander in brain slice-based stroke models."
Author(s): Dunn DE, He DN, Yang P, Johansen M, Newman RA, Lo DC
Number of Citations: 1
(See PubMed article )
  Ms / 1:100
MA3-915 was used in immunocytochemistry and western blot to investigate the effect of mGluR6 on TRPM1 function in retina

J Neurophysiol. 2012 Feb;107(3):948-57.
"mGluR6 deletion renders the TRPM1 channel in retina inactive."
Author(s): Xu Y, Dhingra A, Fina ME, Koike C, Furukawa T, Vardi N
Number of Citations: 1
(See PubMed article )
  Po / 1:4000
MA3-915 was used in immunocytochemistry, immunoprecipitation, and western blot to characterize sodium/potassium ATPases in ventricular myocytes

J Physiol Sci. 2006 Feb;56(1):113-21.
"Subunit composition and role of Na+,K+-ATPases in ventricular myocytes."
Author(s): Harada K, Lin H, Endo Y, Fujishiro N, Sakamoto Y, Inoue M
Number of Citations: 1
(See PubMed article )
  Po / 1:50000
MA3-915 was used in western blot to investigate the effect of 20-hydroxyeicosatetraenoic acid synthesis inhibitor HET0016 on neonatal ischemic brain damage

J Neurochem. 2012 Apr;121(1):168-79.
"Attenuation of neonatal ischemic brain damage using a 20-HETE synthesis inhibitor."
Author(s): Yang ZJ, Carter EL, Kibler KK, Kwansa H, Crafa DA, Martin LJ, Roman RJ, Harder DR, Koehler RC
Number of Citations: 0
(See PubMed article )
  Rb / Not Cited
MA3-915 was used in western blot and immunohistochemistry to study the localization of the sodium pump alpha-isoform in whole lens and within the major structural regions of lens

Hypertension. 1999 Nov;34(5):1168-74.
"Sodium pump inhibition and regional expression of sodium pump alpha-isoforms in lens."
Author(s): Tao QF, Hollenberg NK, Graves SW
Number of Citations: 0
(See PubMed article )
  Rt / Not Cited
MA3-915 was used in western blot to characterize Na,K-ATPase alpha monoclonal antibodies

J Biol Chem. 1996 Sep 20;271(38):23407-17.
"Isoform-specific monoclonal antibodies to Na,K-ATPase alpha subunits. Evidence for a tissue-specific post-translational modification of the alpha subunit."
Author(s): Arystarkhova E, Sweadner KJ
Number of Citations: 8
(See PubMed article )
  Rt / Not Cited
MA3-915 was used in immunohistochemistry and western blot to confirm whether increased levels of the alpha (3) subunit of the Na(+)/K(+)-ATPase correlate with the increased resting membrane potential of developing rat cerebellar neurons

Brain Res Dev Brain Res. 2000 Oct 28;123(2):165-72.
"Quantification of the alpha(3) subunit of the Na(+)/K(+)-ATPase in developing rat cerebellum."
Author(s): Biser PS, Thayne KA, Kong JQ, Fleming WW, Taylor DA
Number of Citations: 2
(See PubMed article )
  Rt / 1:450
MA3-915 was used in western blot to investigate the effect of acute NaCl loading on MBG and OLC in Dahl salt-sensitive (DS) and salt-resistant (DR) rats

Circulation. 2000 Dec 12;102(24):3009-14.
"Endogenous Na,K pump ligands are differentially regulated during acute NaCl loading of Dahl rats."
Author(s): Fedorova OV, Lakatta EG, Bagrov AY
Number of Citations: 9
(See PubMed article )
  Rt / 1:2,000
MA3-915 was used in western blot and immunohistochemistry to study the localization and transport of choline transporters in cholinergic terminals.

J Neurosci. 2003 Oct 29;23(30):9697-709.
"Vesicular localization and activity-dependent trafficking of presynaptic choline transporters."
Author(s): Ferguson SM, Savchenko V, Apparsundaram S, Zwick M, Wright J, Heilman CJ, Yi H, Levey AI, Blakely RD
Number of Citations: 12
(See PubMed article )
  Rt / 1:10000
MA3-915 was used in western blot to investigate the expression level of sodium/potassium ATPase alpha-subunit isoforms in rat resistance vessels

Acta Physiol Scand. 2004 Jan;180(1):49-56.
"Quantification of alpha-subunit isoforms of Na,K-ATPase in rat resistance vessels."
Author(s): N/A
Number of Citations: 0
(See PubMed article )
  Rt / Not Cited
MA3-915 was used in western blot to study the relationship between alpha1 H1-H2 sequence and NKA-contractile modulation

FASEB J. 2005 Jan;19(1):53-61.
"Evidence that the H1-H2 domain of alpha1 subunit of (Na++K+)-ATPase participates in the regulation of cardiac contraction."
Author(s): Xu KY, Takimoto E, Juang GJ, Zhang Q, Rohde H, Myers AC
Number of Citations: 1
(See PubMed article )
  Rt / 1:2000
MA3-915 was used in immunoprecipitation and western blot to study the role of glutamate transporter coupling to Na,K-ATPase in glutamatergic neurotransmission

J Neurosci. 2009 Jun 24;29(25):8143-55.
"Glutamate transporter coupling to Na,K-ATPase."
Author(s): Rose EM, Koo JC, Antflick JE, Ahmed SM, Angers S, Hampson DR
Number of Citations: 15
(See PubMed article )
  Rt / 0
MA3-915 was used in immunocytochemistry and western blot to investigate the expression sodium/potassium ATPase alpha3 in the muscle spindle afferents

J Physiol. 2010 Nov 1;588(Pt 21):4131-43.
"Immunostaining for the ?3 isoform of the Na+/K+-ATPase is selective for functionally identified muscle spindle afferents in vivo."
Author(s): Parekh A, Campbell AJ, Djouhri L, Fang X, McMullan S, Berry C, Acosta C, Lawson SN
Number of Citations: 1
(See PubMed article )
  Rt / 1 ug/ml
MA3-915 was used in western blot to investigate the effect of sodium/potassium ATPase inhibitors on synaptosomal membrane Na+, K+-ATPase activity and alpha 3 subunit expression in central nervous system

Neurochem Res. 2011 Feb;36(2):297-303.
"Changes in Na+, K+-ATPase activity and alpha 3 subunit expression in CNS after administration of Na+, K+-ATPase inhibitors."
Author(s): Bersier MG, Peña C, Arnaiz GR
Number of Citations: 0
(See PubMed article )
  Sh / 1:5000
MA3-915 was used in western blot to characterize the sodium/potassium ATPase from rectal glands of squalus acanthias

Pflugers Arch. 1999 Mar;437(4):517-22.
"Heterogeneity of Na+/K+-ATPase from rectal gland of Squalus acanthias is not due to alpha isoform diversity."
Author(s): N/A
Number of Citations: 2
(See PubMed article )
Immunocytochemistry
  Species / Dilution Summary
  Hu / Not Cited
MA3-915 was used in immunocytochemistry to investigate the localization and ubiquitination status of serum and glucocorticoid-induced protein kinase-1.

J Biol Chem. 2002 Nov 8;277(45):43064-70.
"Ubiquitin modification of serum and glucocorticoid-induced protein kinase-1 (SGK-1)."
Author(s): Brickley DR, Mikosz CA, Hagan CR, Conzen SD
Number of Citations: 1
(See PubMed article )
  Ms / 1:500
MA3-915 was used in immunocytochemistry and western blot to investigate the accumulation of huntingtin and GSK 3-beta mutants in murine Huntington model

J Neurosci Res. 2010 Jan;88(1):179-90.
"Mutant huntingtin and glycogen synthase kinase 3-beta accumulate in neuronal lipid rafts of a presymptomatic knock-in mouse model of Huntington's disease."
Author(s): Valencia A, Reeves PB, Sapp E, Li X, Alexander J, Kegel KB, Chase K, Aronin N, DiFiglia M
Number of Citations: 1
(See PubMed article )
  Ms / 0
MA3-915 was used in immunocytochemistry to investigate the spatial distribution of sodium/potassium-ATPase in dendritic spines

BMC Neurosci. 2011 Jan 27;12():16.
"Spatial distribution of Na+-K+-ATPase in dendritic spines dissected by nanoscale superresolution STED microscopy."
Author(s): Blom H, Rönnlund D, Scott L, Spicarova Z, Widengren J, Bondar A, Aperia A, Brismar H
Number of Citations: 1
(See PubMed article )
  Ms / 0
MA3-915 was used in immunocytochemistry to analyze dopamine D1 receptors and sodium/potassium-ATPases in dendritic spines

Microsc Res Tech. 2012 Feb;75(2):220-8.
"Nearest neighbor analysis of dopamine D1 receptors and Na(+)-K(+)-ATPases in dendritic spines dissected by STED microscopy."
Author(s): Blom H, Rönnlund D, Scott L, Spicarova Z, Rantanen V, Widengren J, Aperia A, Brismar H
Number of Citations: 1
(See PubMed article )
  Ms / 1:100
MA3-915 was used in immunocytochemistry and western blot to investigate the effect of mGluR6 on TRPM1 function in retina

J Neurophysiol. 2012 Feb;107(3):948-57.
"mGluR6 deletion renders the TRPM1 channel in retina inactive."
Author(s): Xu Y, Dhingra A, Fina ME, Koike C, Furukawa T, Vardi N
Number of Citations: 1
(See PubMed article )
  Nhp / Not Cited
Ma3-915 was used in western blot and immunocytochemistry to profile the sodium pump isoforms in human erythroid progenitor cells and mature erythrocytes.

Proc Natl Acad Sci U S A. 2002 Oct 29;99(22):14572-7.
"Na pump isoforms in human erythroid progenitor cells and mature erythrocytes."
Author(s): Hoffman JF, Wickrema A, Potapova O, Milanick M, Yingst DR
Number of Citations: 1
(See PubMed article )
  Po / 1:100
MA3-915 was used in immunocytochemistry, immunoprecipitation, and western blot to characterize sodium/potassium ATPases in ventricular myocytes

J Physiol Sci. 2006 Feb;56(1):113-21.
"Subunit composition and role of Na+,K+-ATPases in ventricular myocytes."
Author(s): Harada K, Lin H, Endo Y, Fujishiro N, Sakamoto Y, Inoue M
Number of Citations: 1
(See PubMed article )
  Rt / 0
MA3-915 was used in immunocytochemistry and western blot to investigate the expression sodium/potassium ATPase alpha3 in the muscle spindle afferents

J Physiol. 2010 Nov 1;588(Pt 21):4131-43.
"Immunostaining for the ?3 isoform of the Na+/K+-ATPase is selective for functionally identified muscle spindle afferents in vivo."
Author(s): Parekh A, Campbell AJ, Djouhri L, Fang X, McMullan S, Berry C, Acosta C, Lawson SN
Number of Citations: 1
(See PubMed article )
  Rt / 1:2000
MA3-915 was used in immunocytochemistry to study the role of Na,K-ATPase isoform alpha3 in CNS neurons co-expressing the ubiquitous Na,K-ATPase isoform alpha1

J Biol Chem. 2013 Jan 25;288(4):2734-43.
"A specific and essential role for Na,K-ATPase ?3 in neurons co-expressing ?1 and ?3."
Author(s): Azarias G, Kruusmägi M, Connor S, Akkuratov EE, Liu XL, Lyons D, Brismar H, Broberger C, Aperia A
Number of Citations: 3
(See PubMed article )
Immunohistochemistry
  Species / Dilution Summary
  GP / 7.5 ug/ml
MA3-915 was used in blocking/activating experiment and immunohistochemistry to investigate opioid tolerance in the locus ceruleus neurons of the guinea pig

J Pharmacol Exp Ther. 2001 Sep;298(3):909-16.
"Cellular depolarization of neurons in the locus ceruleus region of the guinea pig associated with the development of tolerance to opioids."
Author(s): Kong JQ, Meng J, Biser PS, Fleming WW, Taylor DA
Number of Citations: 0
(See PubMed article )
  Ms / 1:100
MA3-915 was used in immunohistochemistry to examine the distribution of sodium/potassium ATPase isoforms in the mouse and rat ocular ciliary epithelium

Invest Ophthalmol Vis Sci. 2001 Mar;42(3):763-9.
"Immunocytochemical localization of NaK-ATPase isoforms in the rat and mouse ocular ciliary epithelium."
Author(s): Wetzel RK, Sweadner KJ
Number of Citations: 3
(See PubMed article )
  Ms / Not Cited
MA3-915 was used in western blot and immunohistochemistry to study the localization and transport of choline transporters in cholinergic terminals.

J Neurosci. 2003 Oct 29;23(30):9697-709.
"Vesicular localization and activity-dependent trafficking of presynaptic choline transporters."
Author(s): Ferguson SM, Savchenko V, Apparsundaram S, Zwick M, Wright J, Heilman CJ, Yi H, Levey AI, Blakely RD
Number of Citations: 12
(See PubMed article )
  Ms / 1:200
MA3-915 was used in western blot and immunohistochemistry to investigate the localization of retinoschisin (RS1) on the surface of retinal photoreceptor and bipolar cells

J Biol Chem. 2007 Nov 9;282(45):32792-801.
"Retinoschisin (RS1), the protein encoded by the X-linked retinoschisis gene, is anchored to the surface of retinal photoreceptor and bipolar cells through its interactions with a Na/K ATPase-SARM1 complex."
Author(s): Molday LL, Wu WW, Molday RS
Number of Citations: 1
(See PubMed article )
  Rb / Not Cited
MA3-915 was used in western blot and immunohistochemistry to study the localization of the sodium pump alpha-isoform in whole lens and within the major structural regions of lens

Hypertension. 1999 Nov;34(5):1168-74.
"Sodium pump inhibition and regional expression of sodium pump alpha-isoforms in lens."
Author(s): Tao QF, Hollenberg NK, Graves SW
Number of Citations: 0
(See PubMed article )
  Rt / Not Cited
MA3-915 was used in immunohistochemistry to investigate the distribution of sodium/potent ATPase isoforms in the rat cerebellum and granule cell cultures

J Neurosci. 1997 May 15;17(10):3488-502.
"Isoforms of Na,K-ATPase alpha and beta subunits in the rat cerebellum and in granule cell cultures."
Author(s): Peng L, Martin-Vasallo P, Sweadner KJ
Number of Citations: 5
(See PubMed article )
  Rt / Not Cited
MA3-915 was used in immunohistochemistry and western blot to confirm whether increased levels of the alpha (3) subunit of the Na(+)/K(+)-ATPase correlate with the increased resting membrane potential of developing rat cerebellar neurons

Brain Res Dev Brain Res. 2000 Oct 28;123(2):165-72.
"Quantification of the alpha(3) subunit of the Na(+)/K(+)-ATPase in developing rat cerebellum."
Author(s): Biser PS, Thayne KA, Kong JQ, Fleming WW, Taylor DA
Number of Citations: 2
(See PubMed article )
  Rt / 1:100
MA3-915 was used in immunohistochemistry to examine the distribution of sodium/potassium ATPase isoforms in the mouse and rat ocular ciliary epithelium

Invest Ophthalmol Vis Sci. 2001 Mar;42(3):763-9.
"Immunocytochemical localization of NaK-ATPase isoforms in the rat and mouse ocular ciliary epithelium."
Author(s): Wetzel RK, Sweadner KJ
Number of Citations: 3
(See PubMed article )
  Rt / 1:15
MA3-915 was used in immunohistochemistry to investigate the expression of the alpha3 isoform of the Na+,K+ -ATPase in rat peripheral nervous system

Neuroscience. 2003;116(4):1069-80.
"Stretch receptor-associated expression of alpha 3 isoform of the Na+, K+-ATPase in rat peripheral nervous system."
Author(s): Dobretsov M, Hastings SL, Sims TJ, Stimers JR, Romanovsky D
Number of Citations: 8
(See PubMed article )
  Rt / 1:500
MA3-915 was used in immunohistochemistry to study the neuronal distribution of Na,K-ATPase alpha subunit in the spiral ganglion and organ of Corti

J Assoc Res Otolaryngol. 2009 Mar;10(1):37-49.
"Distribution of the Na,K-ATPase alpha subunit in the rat spiral ganglion and organ of corti."
Author(s): McLean WJ, Smith KA, Glowatzki E, Pyott SJ
Number of Citations: 1
(See PubMed article )
  Rt / 1:600
MA3-915 was used in immunohistochemistry to investigate the role of BK channel in the regulation of cochlear hair cell function

PLoS One. 2010 Nov 4;5(11):e13836.
"BK channels mediate cholinergic inhibition of high frequency cochlear hair cells."
Author(s): Wersinger E, McLean WJ, Fuchs PA, Pyott SJ
Number of Citations: 1
(See PubMed article )
Immunoprecipitation
  Species / Dilution Summary
  Po / 0
MA3-915 was used in immunocytochemistry, immunoprecipitation, and western blot to characterize sodium/potassium ATPases in ventricular myocytes

J Physiol Sci. 2006 Feb;56(1):113-21.
"Subunit composition and role of Na+,K+-ATPases in ventricular myocytes."
Author(s): Harada K, Lin H, Endo Y, Fujishiro N, Sakamoto Y, Inoue M
Number of Citations: 1
(See PubMed article )
  Rt / 0
MA3-915 was used in immunoprecipitation and western blot to study the role of glutamate transporter coupling to Na,K-ATPase in glutamatergic neurotransmission

J Neurosci. 2009 Jun 24;29(25):8143-55.
"Glutamate transporter coupling to Na,K-ATPase."
Author(s): Rose EM, Koo JC, Antflick JE, Ahmed SM, Angers S, Hampson DR
Number of Citations: 15
(See PubMed article )
Blocking Assay
  Species / Dilution Summary
  GP / 1:10
MA3-915 was used in blocking/activating experiment and immunohistochemistry to investigate opioid tolerance in the locus ceruleus neurons of the guinea pig

J Pharmacol Exp Ther. 2001 Sep;298(3):909-16.
"Cellular depolarization of neurons in the locus ceruleus region of the guinea pig associated with the development of tolerance to opioids."
Author(s): Kong JQ, Meng J, Biser PS, Fleming WW, Taylor DA
Number of Citations: 0
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific