Smad3 Antibody

Smad3 Polyclonal Antibody for Western blot, IF, ICC, IP

>> See 12 other antibodies for SMAD3 (MAD homolog 3; MAD, mothers against decapentaplegic homolog 3; SMA- and MAD-related protein 3; SMAD, mothers against DPP homolog 3; hMAD-3; hSMAD3; mad homolog JV15-2; mad protein homolog; mad3; mothers against DPP homolog 3; mothers against decapentaplegic homolog 3)
Synonyms:
MAD homolog 3; MAD, mothers against decapentaplegic homolog 3; SMA- and MAD-related protein 3; SMAD, mothers against DPP homolog 3; hMAD-3; hSMAD3; mad homolog JV15-2; mad protein homolog; mad3; mothers against DPP homolog 3; mothers against decapentaplegic homolog 3
Entrez Gene ID:
Details
Host / Isotype: Rabbit
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu), Mouse (Ms), Rat (Rt)
Immunogen: Synthetic peptide corresponding to a central region unique to Smad3.
Ordering Information
Pierce Smad3 Antibody
Product #
PA5-17378
Size
100 ul
Price
$250.00
Purchase
Add Smad3 Antibody to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:1000
Immunofluorescence (IF) 1:50
Immunocytochemistry (ICC) 1:50
Immunoprecipitation (IP) 1:25
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Purification: Affinity chromatography
Storage Buffer: 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol
Preservative: no preservative
Storage Conditions: -20°C
Product Specific Information
PA5-17378 targets Smad3 in ICC, IP, and WB applications and shows reactivity with Human, mouse, and Rat samples.

The PA5-17378 immunogen is synthetic peptide corresponding to a central region unique to Smad3.

PA5-17378 detects Smad3 which has a predicted molecular weight of approximately 52 kDa.

Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody may have to be performed for your high content assay.
General Information
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.
Product Images
  • Smad3 Antibody (PA5-17378) in WB
    PA5-17378_WesternBlot.jpg

    Western Blot with anti-Smad3 Polyclonal Antibody (PA5-17378) (PA5-17378_WesternBlot.jpg)

    Western Blot with anti-Smad3 Polyclonal Antibody (PA5-17378)

    Western blot analysis of Smad3 in extracts from HeLa cells transfected with Smad3 using Smad3 polyclonal antibody (Product # PA5-17378).
  • Smad3 Antibody (PA5-17378) in WB
    PA5-17378_WesternBlot_2.jpg

    Western Blot with anti-Smad3 Polyclonal Antibody (PA5-17378) (PA5-17378_WesternBlot_2.jpg)

    Western Blot with anti-Smad3 Polyclonal Antibody (PA5-17378)

    Western blot analysis of Smad3 in extracts from HeLa and B35 cell lines using Smad3 polyclonal antibody (Product # PA5-17378).
  • Smad3 Antibody (PA5-17378) in IF
    PA5-17378_Immunofluorescence.jpg

    Immunofluorescence with anti-Smad3 Polyclonal Antibody (PA5-17378) (PA5-17378_Immunofluorescence.jpg)

    Immunofluorescence with anti-Smad3 Polyclonal Antibody (PA5-17378)

(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific