PA5-17378 targets Smad3 in ICC, IP, and WB applications and shows reactivity with Human, mouse, and Rat samples.
The PA5-17378 immunogen is synthetic peptide corresponding to a central region unique to Smad3.
PA5-17378 detects Smad3 which has a predicted molecular weight of approximately 52 kDa.
Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody may have to be performed for your high content assay.
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.
Smad3 Antibody (PA5-17378) in WB PA5-17378_WesternBlot.jpg
Western Blot with anti-Smad3 Polyclonal Antibody (PA5-17378)