SQSTM1 Antibody

SQSTM1 Polyclonal Antibody for Western blot, IF, IHC

>> See 6 other antibodies for SQSTM1 (Sequestosome-1, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa, Ubiquitin-binding protein p62, EBI3-associated protein of 60 kDa, p60, EBIAP, SQSTM1, ORCA, OSIL)
Synonyms:
Sequestosome-1, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa, Ubiquitin-binding protein p62, EBI3-associated protein of 60 kDa, p60, EBIAP, SQSTM1, ORCA, OSIL
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Rabbit
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu )
Immunogen: Synthetic peptide from the C-terminal region of SQSTM1 conjugated to KLH.
Ordering Information
Pierce SQSTM1 Antibody
Product #
PA5-12092
Size
400 ul
Price
$316.00
Purchase
Add SQSTM1 Antibody to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:1000
Immunofluorescence (IF) 1:50-1:100
Immunohistochemistry (IHC) 1:50-1:100
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: Lot-specific
Purification: Protein G
Storage Buffer: PBS
Preservative: 0.09% sodium azide
Storage Conditions: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Product Specific Information
PA5-12092 targets SQSTM1 in WB, IHC and IF applications and shows reactivity with Human samples. The PA5-12092 immunogen is a synthetic peptide from the C-terminal region of SQSTM1 conjugated to KLH. PA5-12092 detects SQSTM1 which has a predicted molecular weight of approximately 48 kDa.
General Information
This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone.
Product Images
  • SQSTM1 Antibody (PA5-12092) in WB
    SQSTM1_Antibody_PA5-12092_WB_2_112013.jpg

    Western Blot with anti-SQSTM1 Polyclonal Antibody (PA5-12092) (SQSTM1_Antibody_PA5-12092_WB_2_112013.jpg)

    Western Blot with anti-SQSTM1 Polyclonal Antibody (PA5-12092)

    Western blot analysis of SQSTM1 (arrow) using anti-SQSTM1 Polyclonal Antibody (PA5-12092). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SQSTM1 gene (Lane 2).
  • SQSTM1 Antibody (PA5-12092) in WB
    SQSTM1_Antibody_PA5-12092_WB_1_112013.jpg

    Western Blot with anti-SQSTM1 Polyclonal Antibody (PA5-12092) (SQSTM1_Antibody_PA5-12092_WB_1_112013.jpg)

    Western Blot with anti-SQSTM1 Polyclonal Antibody (PA5-12092)

    Western blot analysis of pre-incubated without(lane 1) and with(lane 2) blocking peptide in MCF-7 cell line lysate using anti-SQSTM1 Polyclonal Antibody (PA5-12092).
  • SQSTM1 Antibody (PA5-12092) in IF
    SQSTM1_Antibody_PA5-12092_IF_1_112013.jpg

    Immunofluorescence with anti-SQSTM1 Polyclonal Antibody (PA5-12092) (SQSTM1_Antibody_PA5-12092_IF_1_112013.jpg)

    Immunofluorescence with anti-SQSTM1 Polyclonal Antibody (PA5-12092)

    Immunofluorescence staining using anti-SQSTM1 Polyclonal Antibody (PA5-12092)in Methanol-fixed and PFA fixed HeLa cells.
  • SQSTM1 Antibody (PA5-12092) in IF
    SQSTM1_Antibody_PA5-12092_IF_2_112013.jpg

    Immunofluorescence with anti-SQSTM1 Polyclonal Antibody (PA5-12092) (SQSTM1_Antibody_PA5-12092_IF_2_112013.jpg)

    Immunofluorescence with anti-SQSTM1 Polyclonal Antibody (PA5-12092)

    Immunofluorescent analysis of U251 cells stained with anti-SQSTM1 Polyclonal Antibody (PA5-12092). U251 cells were treated with Chloroquine (50 ?M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), cells were then incubated with anti-SQSTM1 Polyclonal Antibody (PA5-12092) (1:200, 2 h at room temperature). Primary antibody detected by Alexa Fluor® 488 conjugated donkey anti-rabbit secondary antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ?g/ml, 5 min).
  • SQSTM1 Antibody (PA5-12092) in IHC
    SQSTM1_Antibody_PA5-12092_IHC_1_112013.jpg

    Immunohistochemistry with anti-SQSTM1 Polyclonal Antibody (PA5-12092) (SQSTM1_Antibody_PA5-12092_IHC_1_112013.jpg)

    Immunohistochemistry with anti-SQSTM1 Polyclonal Antibody (PA5-12092)

    Immunohistochemical staining of Formalin-fixed and paraffin-embedded human cancer tissue reacted with anti-SQSTM1 Polyclonal Antibody (PA5-12092), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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