SQSTM1 Antibody

SQSTM1 Polyclonal Antibody for Western blot, IF, IHC

>> See 9 other antibodies for SQSTM1 (Sequestosome-1, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa, Ubiquitin-binding protein p62, EBI3-associated protein of 60 kDa, p60, EBIAP, SQSTM1, ORCA, OSIL)
Synonyms:
Sequestosome-1, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa, Ubiquitin-binding protein p62, EBI3-associated protein of 60 kDa, p60, EBIAP, SQSTM1, ORCA, OSIL
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Rabbit
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu)
Immunogen: KLH conjugated synthetic peptide between 317-346 amino acids from the C-terminal region of human SQSTM1 (p62)
Ordering Information
Pierce SQSTM1 Antibody
Product #
PA5-12092
Size
400 ul
Price
$316.00
Purchase
Add SQSTM1 Antibody to your cart.
Tested Applications Dilution *
Western Blot (WB) 1:1000
Immunofluorescence (IF) 1:50-1:100
Immunohistochemistry (IHC) 1:50-1:100
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: Lot-specific
Purification: Protein G
Storage Buffer: PBS
Preservative: 0.09% sodium azide
Storage Conditions: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Product Specific Information
This antibody is predicted to react with mouse and rat based on sequence homology.
General Information
SQSTM1/p62 is an adapter protein which binds ubiquitin and may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. This protein may play a role in titin/TTN downstream signaling in muscle cells, and may also regulate signaling cascades through ubiquitination. This protein is involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels. SQSTM1/p62 also appears to play a role in macroautophagic removal of intracellular protein aggregates. Cellular depletion studies of SQSTM1/p62 have indicated a role for association with LC3 and aggregate proteins in order to facilitate normal formation of the autophagosome.
Product Images
  • SQSTM1 Antibody (PA5-12092) in WB
    SQSTM1_Antibody_PA5-12092_WB_2_112013.jpg

    Western Blot with anti-SQSTM1 Polyclonal Antibody (PA5-12092) (SQSTM1_Antibody_PA5-12092_WB_2_112013.jpg)

    Western Blot with anti-SQSTM1 Polyclonal Antibody (PA5-12092)

    Western blot analysis of SQSTM1 (arrow) using a SQSTM1 polyclonal antibody (Product # PA5-12092) in 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SQSTM1 gene (Lane 2).
  • SQSTM1 Antibody (PA5-12092) in WB
    SQSTM1_Antibody_PA5-12092_WB_1_112013.jpg

    Western Blot with anti-SQSTM1 Polyclonal Antibody (PA5-12092) (SQSTM1_Antibody_PA5-12092_WB_1_112013.jpg)

    Western Blot with anti-SQSTM1 Polyclonal Antibody (PA5-12092)

    Western blot analysis of pre-incubated without (lane 1) and with (lane 2) blocking peptide in MCF-7 cell lysate using a SQSTM1 polyclonal antibody (Product # PA5-12092).
  • SQSTM1 Antibody (PA5-12092) in IF
    SQSTM1_Antibody_PA5-12092_IF_1_112013.jpg

    Immunofluorescence with anti-SQSTM1 Polyclonal Antibody (PA5-12092) (SQSTM1_Antibody_PA5-12092_IF_1_112013.jpg)

    Immunofluorescence with anti-SQSTM1 Polyclonal Antibody (PA5-12092)

    Immunofluorescent analysis using a SQSTM1 polyclonal antibody (Product # PA5-12092)in Methanol-fixed and PFA fixed HeLa cells.
  • SQSTM1 Antibody (PA5-12092) in IF
    SQSTM1_Antibody_PA5-12092_IF_2_112013.jpg

    Immunofluorescence with anti-SQSTM1 Polyclonal Antibody (PA5-12092) (SQSTM1_Antibody_PA5-12092_IF_2_112013.jpg)

    Immunofluorescence with anti-SQSTM1 Polyclonal Antibody (PA5-12092)

    Immunofluorescent analysis of U251 cells using a SQSTM1 polyclonal antibody (Product # PA5-12092). U251 cells were treated with Chloroquine (50 uM, 16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), cells were then incubated with a SQSTM1 polyclonal antibody (Product # PA5-12092) (1:200, 2 hr at room temperature). Primary antibody detected by fluor-conjugated donkey anti-rabbit secondary antibody (green) was used (1:1000, 1hr). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min).
  • SQSTM1 Antibody (PA5-12092) in IHC
    SQSTM1_Antibody_PA5-12092_IHC_1_112013.jpg

    Immunohistochemistry with anti-SQSTM1 Polyclonal Antibody (PA5-12092) (SQSTM1_Antibody_PA5-12092_IHC_1_112013.jpg)

    Immunohistochemistry with anti-SQSTM1 Polyclonal Antibody (PA5-12092)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded human cancer tissue using a SQSTM1 polyclonal antibody (Product # PA5-12092), followed by HRP-conjugated secondary antibody and DAB staining.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
Part of Thermo Fisher Scientific