SMAD4 Antibody (SMD46 (DCS-46))

SMAD4 Monoclonal Antibody for Western blot, IF, IP

>> See 7 other antibodies for SMAD4 (MAD homolog 4, MAD, mothers against decapentaplegic homolog 4,SMAD 4, SMAD, mothers against DPP homolog 4, deleted in pancreatic carcinoma locus 4, deletion target in pancreatic carcinoma 4, hSMAD4, mothers against DPP homolog 4, mothers against decapentaplegic homolog 4, mothers against decapentaplegic, Drosophila, homolog of, 4)
Synonyms:
MAD homolog 4, MAD, mothers against decapentaplegic homolog 4,SMAD 4, SMAD, mothers against DPP homolog 4, deleted in pancreatic carcinoma locus 4, deletion target in pancreatic carcinoma 4, hSMAD4, mothers against DPP homolog 4, mothers against decapentaplegic homolog 4, mothers against decapentaplegic, Drosophila, homolog of, 4
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Mouse / IgG1
Class: Monoclonal
Type: Antibody
Clone: SMD46 (DCS-46)
Tested Species Reactivity: Human (Hu)
Published Species Reactivity: Xenopus laevis (Xl)
Immunogen: Purified recombinant DPC4 protein
Ordering Information
Pierce SMAD4 Antibody (SMD46 (DCS-46))
Product #
MA5-14300
Size
500 ul
Price
$316.00
Purchase
Add SMAD4 Antibody (SMD46 (DCS-46)) to your cart.
Tested Applications Dilution *
Western Blot (WB) 1-2 µg/ml
Immunofluorescence (IF) Assay Dependent
Immunoprecipitation (IP) 2 µg/ml
Published Applications Dilution
Western Blot (WB) See publications below
Immunoprecipitation (IP) See publications below
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form: Liquid
Concentration: 0.2mg/ml
Purification: Protein G
Storage Buffer: PBS, pH 7.4, with 0.2% BSA
Preservative: 0.09% sodium azide
Storage Conditions: 4° C, do not freeze
Product Specific Information
MA5-14300 targets SMAD4 in IF, IP, and WB applications and shows reactivity with Human samples.

The MA5-14300 immunogen is purified recombinant DPC4 protein.
General Information
DPC4 (also named SMAD4; small mothers against decapentaplegic deleted in pancreatic carcinoma). The family of SMAD molecules comprises essential mediators of transforming growth factor beta (TGF-beta) signaling. To date, seven members of this family have been identified, each of which plays a specific and separate role in mediating TGF-beta superfamily gene transcription. At least two different members, Smad2 and Smad4/DPC4, have been implicated in human cancer and appear to have tumor-suppressor functions whose inactivation may play an important role in the histogenesis of pancreatic and colorectal malignancy and possibly other human cancers as well.
Product Images
  • SMAD4 Antibody (MA5-14300) in WB
    82404.jpg

    Western Blot with anti-SMAD4 Monoclonal Antibody [SMD46 (DCS-46)] (MA5-14300) (82404.jpg)

    Western Blot with anti-SMAD4 Monoclonal Antibody [SMD46 (DCS-46)] (MA5-14300)

    HeLa cells were lysed 72 hours after transfection. Cells were transfected with Transfection Reagent alone (Lane 1), 100nM ON-TARGETplus siCONTROL Non-Targeting Pool (Lane 2), or 100nM ONTARGETplus SMAD4 siRNA (Lane 3). Western blot data for Actin is included as a control for equal protein loading.
  • SMAD4 Antibody (MA5-14300) in WB
    MA5-14300_WesternBlot.jpg

    Western Blot with anti-SMAD4 Monoclonal Antibody [SMD46 (DCS-46)] (MA5-14300) (MA5-14300_WesternBlot.jpg)

    Western Blot with anti-SMAD4 Monoclonal Antibody [SMD46 (DCS-46)] (MA5-14300)

    Western blot of SMAD4 using SMAD4 Monoclonal Antibody (MA5-14300) on LS174T Cells.
  • SMAD4 Antibody (MA5-14300) in IP
    MA5-14300_Immunoprecipitation.jpg

    Immunoprecipitation with anti-SMAD4 Monoclonal Antibody [SMD46 (DCS-46)] (MA5-14300) (MA5-14300_Immunoprecipitation.jpg)

    Immunoprecipitation with anti-SMAD4 Monoclonal Antibody [SMD46 (DCS-46)] (MA5-14300)

    Immunoprecipitation of SMAD4 using SMAD4 Monoclonal Antibody (MA5-14300) on Native Human LS174T Cells.
Publications:
Western Blot
  Species / Dilution Summary
  Xl / Not Cited
MA5-14300 was used in immunoprecipitation and western blot to investigate the regulation of the onset of loss of mesodermal competence by Notch signaling during Xenopus development

Mech Dev. 2005 May;122(5):671-80.
"Notch signaling modulates the nuclear localization of carboxy-terminal-phosphorylated smad2 and controls the competence of ectodermal cells for activin A."
Author(s): Abe T, Furue M, Kondow A, Matsuzaki K, Asashima M
Number of Citations: 1
(See PubMed article )
Immunoprecipitation
  Species / Dilution Summary
  Xl / Not Cited
MA5-14300 was used in immunoprecipitation and western blot to investigate the regulation of the onset of loss of mesodermal competence by Notch signaling during Xenopus development

Mech Dev. 2005 May;122(5):671-80.
"Notch signaling modulates the nuclear localization of carboxy-terminal-phosphorylated smad2 and controls the competence of ectodermal cells for activin A."
Author(s): Abe T, Furue M, Kondow A, Matsuzaki K, Asashima M
Number of Citations: 1
(See PubMed article )
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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