SMAD2 / SMAD3 Antibody

(This product has been discontinued.)
Please click the link below to find suitable replacements for this discontinued product.

>> See 9 other antibodies for SMAD2

SMAD2 / SMAD3 Polyclonal Antibody for Western blot

>> See 10 other antibodies for SMAD2 (MADH3, HSPC193)
Synonyms:
MADH3, HSPC193
Entrez Gene ID:
UniProt ID:
Details
Host / Isotype: Rabbit / IgG
Class: Polyclonal
Type: Antibody
Tested Species Reactivity: Human (Hu)
Immunogen: SMAD 2 and 3
Ordering Information
Pierce SMAD2 / SMAD3 Antibody
Product #
82403
Size
120 ul
Price
$259.00
Purchase
(DISCONTINUED)
Tested Applications Dilution *
Western Blot (WB) 1:1000
* Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Form Information
Form information is not available for this product.
Product Specific Information
82403 targets SMAD2/SMAD3 in WB applications and shows reactivity with Human samples.

The 82403 immunogen is sMAD 2 and 3.

82403 detects SMAD2/SMAD3 which has a predicted molecular weight of approximately 50 kDa.
General Information
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants encoding the same protein have been observed.
Product Images
  • SMAD2 / SMAD3 Antibody (82403) in WB
    82403.jpg

    Western Blot with anti-SMAD2  /  SMAD3 Polyclonal Antibody (82403) (82403.jpg)

    Western Blot with anti-SMAD2 / SMAD3 Polyclonal Antibody (82403)

    A549 cells were lysed 72 hours after transfection. Cells were transfected with Transfection Reagent alone (Lane 1), 100nM ON-TARGETplus siCONTROL Non-Targeting Pool (Lane 2), or 100nM ONTARGETplus SMAD3 siRNA (Lane 3). Western blot data for GAPDH is included as a control for equal protein loading.
(This product is for In Vitro experimental use only. Not for resale without express authorization.)
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